高效液相色谱法测定大黄滴眼液中黄芩苷含量
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摘要
目的建立测定大黄滴眼液中黄芩苷含量的高效液相色谱(HPLC)法。方法色谱柱为Waters Symmetry C18柱(250 mm×4. 6 mm,5μm),流动相为甲醇-水-冰醋酸(45∶55∶1),流速为1. 0 m L/min,检测波长为315 nm,进样量为10μL。结果黄芩苷质量浓度在21. 787 9～435. 758 4μg/m L范围内与峰面积线性关系良好(r2=0. 999 9),平均回收率为103. 65%,RSD为1. 89%(n=9)。结论该方法操作简便、灵敏度高、结果准确稳定,分离效果好,可用于大黄滴眼液中黄芩苷的含量测定。
Objective To establish an HPLC method for the content determination of baicalin in Dahuang Eye Drops. Methods Waters Symmetry C18 column( 250 mm × 4. 6 mm,5 μm) was adopted,the mobile phase was acetonitrile-water acetic acid( 45 ∶ 55 ∶ 1),the flow rate was 1. 0 m L/min,the detection wavelength was 315 nm,and the sample size was 10 μL. Results The baicalin showed a good linear relationship with the peak area in the range of 21. 787 9-435. 758 4 μg/m L( r2 = 0. 999 9),the average recovery rate was 103. 65%,RSD was 1. 89%( n = 9). Conclusion The method is simple,sensitive,accurate and stable,which can be used for the content determination of baicalin in Dahuang Eye Drops.
Objective To establish an HPLC method for the content determination of baicalin in Dahuang Eye Drops. Methods Waters Symmetry C18 column( 250 mm × 4. 6 mm,5 μm) was adopted,the mobile phase was acetonitrile-water acetic acid( 45 ∶ 55 ∶ 1),the flow rate was 1. 0 m L/min,the detection wavelength was 315 nm,and the sample size was 10 μL. Results The baicalin showed a good linear relationship with the peak area in the range of 21. 787 9-435. 758 4 μg/m L( r2 = 0. 999 9),the average recovery rate was 103. 65%,RSD was 1. 89%( n = 9). Conclusion The method is simple,sensitive,accurate and stable,which can be used for the content determination of baicalin in Dahuang Eye Drops.
引文
[1]郑勇凤,王佳婧,傅超美,等.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.
[2]雷芳.黄芩苷药理作用研究进展[J].中国药业,2010,19(15):87-90.
[3]汪映宇.大黄滴眼液质量标准研究[J].西北药学杂志,2015,30(3):241-244.
[4]国家药典委员会.中华人民共和国药典(四部)[M].北京:中国医药科技出版社,2015:8.
[5]邱天宝,郝哲. HPLC法测定麻黄鱼腥草散中黄芩苷的含量[J].中兽医医药杂志,2017,36(1):36-38.
[6]王敏,贺蕊,龚慕辛,等. HPLC法测定银黄片中黄芩苷和绿原酸的溶出度[J].世界中医药,2017,12(2):408-410.
[7]李玟蔚,罗思婧,江伟鹏.高效液相色谱法测定复方金银花颗粒中黄芩苷的含量[J].海峡药学,2017,29(1):54-56.
[8]金伟华,于波涛,陈华,等.医院制剂感冒颗粒质量标准研究[J].药学实践杂志,2016,34(6):534-536.
[9]赵磊,于丹,赵宾. HPLC法测定复方杜仲丸中盐酸水苏碱与黄芩苷的含量[J].中国药物评价,2017,34(2):86-88.
[10]郝福,李东,胡向青,等.变波长HPLC法同时测定金柴胶囊中绿原酸和黄芩苷含量[J].亚太传统医药,2017,13(8):55-57.
[11]王东超,魏颖,高佳琪,等. HPLC同时测定糖痹康颗粒中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的含量[J].吉林中医药,2017,37(5):513-517.
[12]鲁寅生,梁艳. RP-HPLC法测定小儿柴桂退热颗粒中芍药苷和黄芩苷的含量[J].西北药学杂志,2017,32(3):313-315.
[13]吴一凡.高效液相色谱法测定蒲地蓝口服液中黄芩苷的含量[J].湖北中医杂志,2017,39(5):61-62.
[14]鄢长余,姜范成,于远洋. HPLC法测定炎可宁片中黄芩苷的含量[J].世界最新医学信息文摘,2017,17(27):96-97.
[15]何选林,王群英. RP-HPLC法同时测定三黄片中7种成分[J].中成药,2017,39(7):1522-1524.
[2]雷芳.黄芩苷药理作用研究进展[J].中国药业,2010,19(15):87-90.
[3]汪映宇.大黄滴眼液质量标准研究[J].西北药学杂志,2015,30(3):241-244.
[4]国家药典委员会.中华人民共和国药典(四部)[M].北京:中国医药科技出版社,2015:8.
[5]邱天宝,郝哲. HPLC法测定麻黄鱼腥草散中黄芩苷的含量[J].中兽医医药杂志,2017,36(1):36-38.
[6]王敏,贺蕊,龚慕辛,等. HPLC法测定银黄片中黄芩苷和绿原酸的溶出度[J].世界中医药,2017,12(2):408-410.
[7]李玟蔚,罗思婧,江伟鹏.高效液相色谱法测定复方金银花颗粒中黄芩苷的含量[J].海峡药学,2017,29(1):54-56.
[8]金伟华,于波涛,陈华,等.医院制剂感冒颗粒质量标准研究[J].药学实践杂志,2016,34(6):534-536.
[9]赵磊,于丹,赵宾. HPLC法测定复方杜仲丸中盐酸水苏碱与黄芩苷的含量[J].中国药物评价,2017,34(2):86-88.
[10]郝福,李东,胡向青,等.变波长HPLC法同时测定金柴胶囊中绿原酸和黄芩苷含量[J].亚太传统医药,2017,13(8):55-57.
[11]王东超,魏颖,高佳琪,等. HPLC同时测定糖痹康颗粒中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的含量[J].吉林中医药,2017,37(5):513-517.
[12]鲁寅生,梁艳. RP-HPLC法测定小儿柴桂退热颗粒中芍药苷和黄芩苷的含量[J].西北药学杂志,2017,32(3):313-315.
[13]吴一凡.高效液相色谱法测定蒲地蓝口服液中黄芩苷的含量[J].湖北中医杂志,2017,39(5):61-62.
[14]鄢长余,姜范成,于远洋. HPLC法测定炎可宁片中黄芩苷的含量[J].世界最新医学信息文摘,2017,17(27):96-97.
[15]何选林,王群英. RP-HPLC法同时测定三黄片中7种成分[J].中成药,2017,39(7):1522-1524.