外周血循环肿瘤细胞在49例鼻咽癌中表达
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摘要
[目的]分析外周血循环肿瘤细胞在鼻咽癌中的表达及其临床意义。[方法]采用CanPatrol~(TM)循环肿瘤细胞分型检测技术检测循环肿瘤细胞在49例初治鼻咽癌和20例正常人中的表达情况,分析循环肿瘤细胞的表达与分期和EB病毒的关系。[结果]鼻咽癌患者中循环肿瘤细胞阳性率为57.1%,而正常人阳性率为0,差异有统计学意义(χ~2=16.991,P<0.05)。M_0期和M_1期患者中,循环肿瘤细胞阳性率分别为48.8%和87.5%,差异有统计学意义(χ~2=6.853,P=0.008);EBV-DNA阴性和阳性患者中,阳性率分别为39.4%和88.2%,差异有统计学意义(χ~2=6.983,P=0.013)。[结论]循环肿瘤细胞与鼻咽癌有无全身转移和EBV-DNA密切相关,可用于鼻咽癌病情的评估。
[Objective] To analyze the expression of circulating tumor cells(CTCs) in patients with nasopharyngeal carcinoma(NPC) and its clinical significance. [Methods] The expression of CTCs in 49 non-treated NPC patients and 20 healthy persons was detected by CanPatrolTM CTC detection technology. The relationship of CTCs expression with stage and EB virus were analyzed. [Results] The positive rate of CTCs in NPC patients and healthy person was 57.1% and 0 respectively(χ~2=16.991,P<0.05).The positive rate in M_0 and M_1 NPC patients was 48.8% and 87.5% respectively(χ~2=6.853,P =0.008). The positive rate in NPC patients with negative EBV-DNA and positive EBV-DNA was 39.4% and 88.2%(χ~2=6.983,P=0.013).[Conclusion] CTCs are closely related to systemic metastasis and EBV-DNA of nasopharyngeal carcinoma,it can be used to evaluate the condition of nasopharyngeal carcinoma.
[Objective] To analyze the expression of circulating tumor cells(CTCs) in patients with nasopharyngeal carcinoma(NPC) and its clinical significance. [Methods] The expression of CTCs in 49 non-treated NPC patients and 20 healthy persons was detected by CanPatrolTM CTC detection technology. The relationship of CTCs expression with stage and EB virus were analyzed. [Results] The positive rate of CTCs in NPC patients and healthy person was 57.1% and 0 respectively(χ~2=16.991,P<0.05).The positive rate in M_0 and M_1 NPC patients was 48.8% and 87.5% respectively(χ~2=6.853,P =0.008). The positive rate in NPC patients with negative EBV-DNA and positive EBV-DNA was 39.4% and 88.2%(χ~2=6.983,P=0.013).[Conclusion] CTCs are closely related to systemic metastasis and EBV-DNA of nasopharyngeal carcinoma,it can be used to evaluate the condition of nasopharyngeal carcinoma.
引文
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[19] Wu JX,He JY,Du KX,et al. Detection of circulating tumor cells in peripheral blood of patients with nasopharyngeal carcinoma and its correlation with prognosis[J]. Modern Oncology Medicine,2017,25(7):1035-1039.[吴君心,贺俊彦,杜开新,等.鼻咽癌患者外周血循环肿瘤细胞的检测及其与预后的相关性[J].现代肿瘤医学,2017,25(7):1035-1039.]
[2] Ng WT,Lee MC,Hung WM,et al. Clinical outcomes and patterns offailure after intensity-modulated radiotherapy for nasopharyngeal carcinoma[J]. Int J Radiat Oncol Biol Phy,2011,79(2):420-428.
[3] Attard G,De Bono JS. Utilizing circulating tumor cells:challenges and pitfalls[J]. Curr Opin Genet Dev,2011,21(1):50-58.
[4] Kim MY,Oskarsson T,Acharyya S,et al. Tumor self-seeding by circulating cancer cells[J]. Cell,2009,139(7):1315-1326.
[5] Ross JS,Slodkowska EA. Circulating and disseminated tumor cells in the management of breast cancer[J]. Am J Clin Pathol,2009,132(2):237-245.
[6] Dorsey JF,Kao GD,MacArthur KM,et al. Tracking viable circulating tumor cells(CTCs)in the peripheral blood of non-small cell lung cancer(NSCLC)patients undergoing definitive radiation therapy:pilot study results[J]. Cancer,2015,121(1):139-149.
[7] Mu Z,Wang C,Ye Z,et al. Prospective assessment of the prognostic value of circulating tumor cells and their clusters in patients with advanced-stage breast cancer[J].Breast Cancer Res Treat,2015,154(3):563-571.
[8] van Dalum G,Stam GJ,Scholten LF,et al. Importance of circulating tumor cells in newly diagnosed colorectal cancer[J]. Int J Oncol,2015,46(3):1361-1368.
[9] Huang X,Gao P,Sun J,et al. Clinicopathological and prognostic significance of circulating tumor cells in patients with gastric cancer:a meta-analysis[J]. Int J Cancer,2015,136(1):21-33.
[10] Zhang Y,Wang F,Ning N,et al. Patterns of circulating tumor cells identified by CEP8,CK and CD45 in pancreatic cancer[J]. Int J Cancer,2015,136(5):1228-1233.
[11] China Working Committee on Clinical Staging of Nasopharyngeal Carcinoma. Report on the revision of 92stages of nasopharyngeal carcinoma[J]. Chinese Journal of Radiation Oncology,2009,18(1):2-6.[中国鼻咽癌临床分期工作委员会.鼻咽癌92分期修订工作报告[J].中华放射肿瘤学杂志,2009,18(1):2-6.]
[12] Chen W,Zheng R,Baade PD,et al. Cancer statistics in China,2015[J]. CA:A Cancer J Clin,2016,66(2):115-132.
[13] Mehta S,Shelling A,Muthukaruppan A,et al. Predictive and prognostic molecular markers for cancer medicine[J].Ther Adv Med Oncol,2010,2(2):125-148.
[14] Ashworth T. A case of cancer in which cells similar to those in the tumours were seen in the blood after death[J].Austral Med J,1869,20(14):146-147.
[15] Wu S,Liu S,Liu Z,et al. Classification of circulating tumor cells by epithelial-mesenchymal transition markers[J]. PLoS One,2015,10(4):76-81.
[16] Alix-Panabieres C,Pantel K. Circulating tumor cells:liquid biopsy of cancer[J]. Clin Chem,2013,59(1):110-118.
[17] Cai QQ,Ding Y,Lin TY,et al. Detection of circulating tumor cells in peripheral blood of patients with nasopharyngeal carcinoma by modified IME-FICC and its clinical significance[J]. Chinese Journal of Pathophysiology,2011,27(10):1896-1901.[蔡清清,丁颖,林天歆,等.改良IME-FICC法检测鼻咽癌患者外周血循环肿瘤细胞及其临床意义分析[J].中国病理生理杂志,2011,27(10):1896-1901.]
[18] Federico A,Orditura M,Cotticelli G,et al. Safety and efficacy of sorafenib in patients with advanced hepatocellular carcinoma and Child-pugh A or B cirrhosis[J]. Oncol Lett,2015,9(4):1628-1632.
[19] Wu JX,He JY,Du KX,et al. Detection of circulating tumor cells in peripheral blood of patients with nasopharyngeal carcinoma and its correlation with prognosis[J]. Modern Oncology Medicine,2017,25(7):1035-1039.[吴君心,贺俊彦,杜开新,等.鼻咽癌患者外周血循环肿瘤细胞的检测及其与预后的相关性[J].现代肿瘤医学,2017,25(7):1035-1039.]