F_(2∶3)家系法鉴定大白菜TuMV分离群体单株抗性
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摘要
为筛选与目的基因连锁更加准确的分子标记以及明确分子标记鉴定技术的准确率,同时为后期进行基因精细定位研究提供技术手段,本试验采用F_(2∶3)家系进行F_2单株的TuMV抗性鉴定。结果表明,122个大白菜抗TuMV材料8407和感病材料冠291的F_(2∶3)家系中,抗病的有98个,感病的有24个,经卡方测验,符合3∶1的分离比例,与以往对该群体F_2代TuMV抗性分离比例的研究一致,表明这种鉴定方法可行。本结果可为今后分离群体单株TuMV抗性的准确鉴定提供参考。
In order to screen out molecular markers with more accurate linkage distance and make clear of the identification accuracy of molecular markers, as well as for fine gene mapping in the later period, the F_(2∶3) families were used to identify the TuMV resistance of F_2 single plant. The results showed that in 122 Chinese cabbage F_(2∶3) families with their resistant parent ‘8407' and susceptible parent ‘Guan 291', 98 were resistant families and 24 were susceptible families,which was in accordance with the 3∶1 separation ratio by Chi-square test. This was consistent with previous studies in segregation ratio of TuMV resistance in F_2 generation, which indicated that this method was feasible. This study provided a methodological guide for accurate identification of individual isolate on TuMV resistance in the future.
In order to screen out molecular markers with more accurate linkage distance and make clear of the identification accuracy of molecular markers, as well as for fine gene mapping in the later period, the F_(2∶3) families were used to identify the TuMV resistance of F_2 single plant. The results showed that in 122 Chinese cabbage F_(2∶3) families with their resistant parent ‘8407' and susceptible parent ‘Guan 291', 98 were resistant families and 24 were susceptible families,which was in accordance with the 3∶1 separation ratio by Chi-square test. This was consistent with previous studies in segregation ratio of TuMV resistance in F_2 generation, which indicated that this method was feasible. This study provided a methodological guide for accurate identification of individual isolate on TuMV resistance in the future.
引文
[1] 魏蒙关.玉米两个相关F2∶3群体秸秆产量和品质性状QTL分析及遗传相关研究[D].郑州:河南农业大学,2009.
[2] 余学杰,石海春,柯永培,等. 一个玉米F2∶3群体主要性状的变异及相关分析[J]. 玉米科学,2008 ,16(3): 22-25.
[3] 李清超,马浪浪,文琼,等. 基于玉米F2∶3群体株型性状的灰色关联度分析[J]. 广东农业科学,2015,42(21):6-10.
[4] 周坤华,雷刚,方荣,等. 利用辣椒种间 F2 和F2∶3两个群体进行其主要农艺性状 QTL 分析[J]. 园艺学报,2015,42(5):879-889.
[5] 魏良明,王延召,宋迎辉,等. 玉米F2∶3群体淀粉含量的表现及相关性分析[J]. 种业导刊,2016(12):15-17.
[6] 蒋思霞,陈坚剑,徐志英,等. 玉米自交系和F2∶3家系对黄曲霉菌抗性的鉴定[J]. 扬州大学学报(农业与生命科学版),2012,33(2):45-50.
[7] 钱伟.大白菜TuMV抗性基因retr02的克隆与抗性机制研究[D].北京:中国农业科学院,2013.
[8] 李巧云,张志刚,赵智中,等. 大白菜抗TuMV分子标记辅助选择技术研究[J].山东农业科学,2017,49(2): 10-14.
[9] 李巧云,张志刚,成文华,等.利用ELISA方法鉴定大白菜TuMV抗性[J].科技导报,2009,27(1):42-45.
[10] 高会超,曾强,张志刚,等. 与大白菜TuMV抗病基因TuRBCS01紧密连锁的分子标记开发[J].农业生物技术学报,2016, 24(2): 196-205.
[11] 张晓亮.大白菜抗芜菁花叶病毒(TuMV)基因分子标记筛选与定位[D].青岛:青岛农业大学,2012.
[12] 国家质量监督检验检疫总局,中国国家标准化管理委员会. 植物新品种特异性、一致性和稳定性测试指南大白菜:GB/T 19557.5—2004 [S].北京:中国标准出版社,2004.
[2] 余学杰,石海春,柯永培,等. 一个玉米F2∶3群体主要性状的变异及相关分析[J]. 玉米科学,2008 ,16(3): 22-25.
[3] 李清超,马浪浪,文琼,等. 基于玉米F2∶3群体株型性状的灰色关联度分析[J]. 广东农业科学,2015,42(21):6-10.
[4] 周坤华,雷刚,方荣,等. 利用辣椒种间 F2 和F2∶3两个群体进行其主要农艺性状 QTL 分析[J]. 园艺学报,2015,42(5):879-889.
[5] 魏良明,王延召,宋迎辉,等. 玉米F2∶3群体淀粉含量的表现及相关性分析[J]. 种业导刊,2016(12):15-17.
[6] 蒋思霞,陈坚剑,徐志英,等. 玉米自交系和F2∶3家系对黄曲霉菌抗性的鉴定[J]. 扬州大学学报(农业与生命科学版),2012,33(2):45-50.
[7] 钱伟.大白菜TuMV抗性基因retr02的克隆与抗性机制研究[D].北京:中国农业科学院,2013.
[8] 李巧云,张志刚,赵智中,等. 大白菜抗TuMV分子标记辅助选择技术研究[J].山东农业科学,2017,49(2): 10-14.
[9] 李巧云,张志刚,成文华,等.利用ELISA方法鉴定大白菜TuMV抗性[J].科技导报,2009,27(1):42-45.
[10] 高会超,曾强,张志刚,等. 与大白菜TuMV抗病基因TuRBCS01紧密连锁的分子标记开发[J].农业生物技术学报,2016, 24(2): 196-205.
[11] 张晓亮.大白菜抗芜菁花叶病毒(TuMV)基因分子标记筛选与定位[D].青岛:青岛农业大学,2012.
[12] 国家质量监督检验检疫总局,中国国家标准化管理委员会. 植物新品种特异性、一致性和稳定性测试指南大白菜:GB/T 19557.5—2004 [S].北京:中国标准出版社,2004.