重庆玉米DH工程化育种的进展分析
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摘要
玉米双单倍体(doubled haploid,DH)技术是生物技术与常规育种方法结合产生的一条新的育种途径,能显著加快育种进程。在重庆地区,土壤瘠薄、阴雨寡照、高温干旱等一系列特殊的生态气候因素严重制约重庆玉米DH工程化育种的开展。本综述就重庆玉米DH工程化技术的关键环节进行了探索,包括对诱导系的选择、基础材料的组建、单倍体的诱导、加倍、DH鉴定及应用,总结出一套适宜重庆本地的玉米DH工程化育种体系,提出"目标具体,规模加倍,分圃鉴定,测评应用"的单倍体育种策略,为玉米新型商业化育种体系建立提供参考依据。
The double haploid(DH) technology in maize(Zea mays L.) is a new means for accelerating the breeding process with biotechnology and routine breeding. Study on bio-haploid maize breeding in Chongqing facing some special constraints such as barren soil, high temperature and drought, cloudy and drizzly ecoclimatic conditions limit.This paper analyses the key step of selection of inducer, establishment of source germplasm, induction, doubling,DH identification and application of haploids, summarizes that there is an optimum bio-haploid breeding schemes for local maize, and put forward strategies on haploid breeding that"nailing down direction, focusing on doubling,identifying by fraction, using with evaluation"and is willing to establish a new commercial maize breeding system for reference.
The double haploid(DH) technology in maize(Zea mays L.) is a new means for accelerating the breeding process with biotechnology and routine breeding. Study on bio-haploid maize breeding in Chongqing facing some special constraints such as barren soil, high temperature and drought, cloudy and drizzly ecoclimatic conditions limit.This paper analyses the key step of selection of inducer, establishment of source germplasm, induction, doubling,DH identification and application of haploids, summarizes that there is an optimum bio-haploid breeding schemes for local maize, and put forward strategies on haploid breeding that"nailing down direction, focusing on doubling,identifying by fraction, using with evaluation"and is willing to establish a new commercial maize breeding system for reference.
引文
Cai Z.,Xu G.L.,Liu X.H.,D ong Y.L.,and Li S.H.,2007,The breeding of JAAS3-haploid inducer with high frequency parthenogenes is in maize,Yumi Kexue(Journal of Maize Sciences),15(1):1-4(才卓,徐国良,刘向辉,李淑华,2007,玉米高频率单倍生殖诱导系吉高诱系3号的选育,玉米科学,15(1):1-4)
Cai Z.,Xu G.L.,Ren J.,Dai Y.X.,Yu M.Y.,Li S.H.,Liu X.D.,Guo Q.,Wang L.N.,and Zhang M.T.,2016,Selection experiment of high spontaneous male fertility restorer frequency maize population,Yumi Kexue(Journal of Maize Sciences),24(4):1-6(才卓,徐国良,任军,代玉仙,于明艳,李淑华,刘小丹,郭琦,王丽娜,张銘堂,2016,玉米单倍体雄穗自然加倍性轮选遗传修复与高加倍率材料的创制,玉米科学,24(4):1-6)
Chen S.J.,and Song T.M.,2003,Identification haploid with high oil xenia effect in maize,Zuowu Xuebao(Acta Agronom Ica Sinica),29(4):587-590(陈绍江,宋同明,2003,利用高油分的花粉直感效应鉴别玉米单倍体,作物学报,29(4):587-590)
Coe E.H.,1959,A line of maize with high haploid frequency,Am.Nat.,93(873):381-382
Geiger H.H.,and Gordillo G.A.,2009,Doubled haploids in hybrid maize breeding,Maydica,54(4):485
Kermicle J.L.,1969,Androgenesis conditioned by a mutation in maize,Science,166(3911):1422-1424
Kelliher T.,Starr D.,Richbourg L.,Chintamanani S.,Delzer B.,Nuccio,M.L.,Green J.,Chen Z.Y.,Mc Cuiston J.,Wang W.L.,Liebler T.,Bullock P.,and Martin B.,2017,MATRI-LINEAL,a sperm-specific phospholipase,triggers maize haploid induction,Nature,542(7639):105-109
Lashermes P.,and Beckert M.,1988,Genetic control of maternal haploidy in maize(Zea mays L.)and selection of haploid inducing lines,Theor.Appl.Genet.,76(3):405-410
Liu C.X,Li W.,Zhong Y.,Dong X.,Hu H.X.,Tian X.L.,Chen B.J.,and Chen C.,2015,Fine mapping of qhir8 affecting in vivo haploid induction in maize,Theor.Appl.Genet.,128(12):2507-2515
Liu C.X.,Li X.,Meng D.X,Zhong Y.,Chen C.,Dong,X.,Xu X.W.,Chen B.J.,Li W.,Li L.,Tian X.L.,Zhao H.M.,Song W.B.,Luo H.S.,Zhang Q.H.,Lai J.S.,and Chen S.J.,2017,A4 bp insertion at Zm PLA1 encoding a putative phospholipase a generates haploid induction in maize,Mol.Plant,10(3):520-522
Ma J.,Jiang M.,Liu X.F.,and Wang H.,2011,Discussions on haploid breeding in maize,Dongbei Nongye Daxue Xuebao(Journal of Northeast Agricultural University),42(10):135-139(马骏,姜敏,刘欣芳,王贺,2011,玉米单倍体育种研究技术的探讨,东北农业大学学报,42(10):135-139)
Ma H.L.,Li J.R.,Lu B.H.,Guo S.,and Zhang L.,2014,The main points of haploid technology and its application in maize breeding,Nongxue Xuebao(Journal of Agriculture),4(9):25-29(马海林,李继荣,卢保红,郭盛,张璐,2014,玉米单倍体技术要点及其在育种中的应用展望,农学学报,4(9):25-29)
Melchinger A.E.,Schipprack W.,Würschum T.,Chen S.,and Technow F.,2013,Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize,Sci.Rep.,3(3):21-29
Prasanna B.M.,Chaikam V.,and Mahuku G.,2012,Doubled haploid(DH)technology in maize breeding:an overview,Maydica,54:1-8
Qi Z.Y.,Yang H.,Qiu Z.G.,Zhang Y.Q.,Yuan L.,Cai Z.R.,Wang N.,Jin C.,and Li S.J.,2012,Study on induction effect of haploid in different maize genotypes,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),25(4):1152-1158(祁志云,杨华,邱正高,张亚勤,袁亮,蔡治荣,王楠,金川,李淑君,2012,不同基因型玉米单倍体诱导效果研究,西南农业学报,25(4):1152-1158)
Qi Z.Y.,Yang H.,Qiu Z.G.,Zhang Y.Q.,Li S.J.,Yuan L.,and Jin C.,2013,A study of the influence of different processing methods on maize haploid doubling effificiency,Nanfang Nongye(South China Agriculture),7(8):61-63(祁志云,杨华,邱正高,张亚勤,李淑君,袁亮,金川,2013,不同处理方法对玉米单倍体加倍效率的影响研究,南方农业,7(8):61-63)
Zhang X.Q.,Wang S.J.,and Sun M.,2015,Dynamic responses of active oxygen metabolism of Barbula fallax in rock desertification of Karst areas to drought stress,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(1):160-164(张显强,王世杰,孙敏,2015,喀斯特石生反叶扭口藓活性氧代谢对干旱胁迫的动态响应,基因组学与应用生物学,34(1):160-164)
Cai Z.,Xu G.L.,Ren J.,Dai Y.X.,Yu M.Y.,Li S.H.,Liu X.D.,Guo Q.,Wang L.N.,and Zhang M.T.,2016,Selection experiment of high spontaneous male fertility restorer frequency maize population,Yumi Kexue(Journal of Maize Sciences),24(4):1-6(才卓,徐国良,任军,代玉仙,于明艳,李淑华,刘小丹,郭琦,王丽娜,张銘堂,2016,玉米单倍体雄穗自然加倍性轮选遗传修复与高加倍率材料的创制,玉米科学,24(4):1-6)
Chen S.J.,and Song T.M.,2003,Identification haploid with high oil xenia effect in maize,Zuowu Xuebao(Acta Agronom Ica Sinica),29(4):587-590(陈绍江,宋同明,2003,利用高油分的花粉直感效应鉴别玉米单倍体,作物学报,29(4):587-590)
Coe E.H.,1959,A line of maize with high haploid frequency,Am.Nat.,93(873):381-382
Geiger H.H.,and Gordillo G.A.,2009,Doubled haploids in hybrid maize breeding,Maydica,54(4):485
Kermicle J.L.,1969,Androgenesis conditioned by a mutation in maize,Science,166(3911):1422-1424
Kelliher T.,Starr D.,Richbourg L.,Chintamanani S.,Delzer B.,Nuccio,M.L.,Green J.,Chen Z.Y.,Mc Cuiston J.,Wang W.L.,Liebler T.,Bullock P.,and Martin B.,2017,MATRI-LINEAL,a sperm-specific phospholipase,triggers maize haploid induction,Nature,542(7639):105-109
Lashermes P.,and Beckert M.,1988,Genetic control of maternal haploidy in maize(Zea mays L.)and selection of haploid inducing lines,Theor.Appl.Genet.,76(3):405-410
Liu C.X,Li W.,Zhong Y.,Dong X.,Hu H.X.,Tian X.L.,Chen B.J.,and Chen C.,2015,Fine mapping of qhir8 affecting in vivo haploid induction in maize,Theor.Appl.Genet.,128(12):2507-2515
Liu C.X.,Li X.,Meng D.X,Zhong Y.,Chen C.,Dong,X.,Xu X.W.,Chen B.J.,Li W.,Li L.,Tian X.L.,Zhao H.M.,Song W.B.,Luo H.S.,Zhang Q.H.,Lai J.S.,and Chen S.J.,2017,A4 bp insertion at Zm PLA1 encoding a putative phospholipase a generates haploid induction in maize,Mol.Plant,10(3):520-522
Ma J.,Jiang M.,Liu X.F.,and Wang H.,2011,Discussions on haploid breeding in maize,Dongbei Nongye Daxue Xuebao(Journal of Northeast Agricultural University),42(10):135-139(马骏,姜敏,刘欣芳,王贺,2011,玉米单倍体育种研究技术的探讨,东北农业大学学报,42(10):135-139)
Ma H.L.,Li J.R.,Lu B.H.,Guo S.,and Zhang L.,2014,The main points of haploid technology and its application in maize breeding,Nongxue Xuebao(Journal of Agriculture),4(9):25-29(马海林,李继荣,卢保红,郭盛,张璐,2014,玉米单倍体技术要点及其在育种中的应用展望,农学学报,4(9):25-29)
Melchinger A.E.,Schipprack W.,Würschum T.,Chen S.,and Technow F.,2013,Rapid and accurate identification of in vivo-induced haploid seeds based on oil content in maize,Sci.Rep.,3(3):21-29
Prasanna B.M.,Chaikam V.,and Mahuku G.,2012,Doubled haploid(DH)technology in maize breeding:an overview,Maydica,54:1-8
Qi Z.Y.,Yang H.,Qiu Z.G.,Zhang Y.Q.,Yuan L.,Cai Z.R.,Wang N.,Jin C.,and Li S.J.,2012,Study on induction effect of haploid in different maize genotypes,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),25(4):1152-1158(祁志云,杨华,邱正高,张亚勤,袁亮,蔡治荣,王楠,金川,李淑君,2012,不同基因型玉米单倍体诱导效果研究,西南农业学报,25(4):1152-1158)
Qi Z.Y.,Yang H.,Qiu Z.G.,Zhang Y.Q.,Li S.J.,Yuan L.,and Jin C.,2013,A study of the influence of different processing methods on maize haploid doubling effificiency,Nanfang Nongye(South China Agriculture),7(8):61-63(祁志云,杨华,邱正高,张亚勤,李淑君,袁亮,金川,2013,不同处理方法对玉米单倍体加倍效率的影响研究,南方农业,7(8):61-63)
Zhang X.Q.,Wang S.J.,and Sun M.,2015,Dynamic responses of active oxygen metabolism of Barbula fallax in rock desertification of Karst areas to drought stress,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),34(1):160-164(张显强,王世杰,孙敏,2015,喀斯特石生反叶扭口藓活性氧代谢对干旱胁迫的动态响应,基因组学与应用生物学,34(1):160-164)