N-CTS/MWNTs/GCE电化学传感器对多巴胺和槲皮素的检测
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摘要
通过N-酰化壳聚糖(N-CTS)与多壁碳纳米管(MWNTs)复合修饰玻碳电极得到N-CTS/MWNTs/GCE电化学传感器。采用循环伏安法研究了多巴胺(DA)和槲皮素(QU)在修饰电极上电化学行为。结果表明:NCTS/MWNTs/GCE电极能显著提高DA、QU的氧化峰电流,降低其氧化峰电位。在p H分别为7.38、6.80磷酸盐缓冲溶液中,DA、QU的氧化峰电流与浓度存在线性关系,线性方程分别为:Ip(DA)=0.0397+4715.8673 c、Ip(QU)=-0.2645+256.8935 c,相关系数均大于0.997,多巴胺、槲皮素检测限分别达1.0×10-8mol·L~(-1)和1.0×10-6mol·L~(-1)。N-CTS/MWNTs/GCE电极具有较好的重现性、稳定性,相对标准偏差为1.51%。该修饰电极可用于含DA、QU成分药物的直接测定。
A molecularly electrochemical sensor( N-CTS/MWNTs/GCE) was prepared using N-acyl chitosan( N-CTS) and multi walled carbon nanotubes( MWNTs) modified glassy carbon electrode. Cyclic voltammetry was applied to study the electrochemical behaviors of dopamine( DA) and quercetin( QU) on the modified N-CTS/MWNTs/GCE electrode Results showed that N-CTS/MWNTs/GCE could greatly improve the oxidation peak currents of both DA and QU.Linear response relationship between the peak current and the concentration of analytes could be established for dopamine and quercetin,as respectively measured in pH = 7. 38,6. 80 phosphate buffer solution.Linear equations can be described as followed: Ip( DA)= 0. 0397+ 4715. 8673 c and Ip( QU)=-0. 2645+256. 8935 c.The correlation coefficients for these two equations are all larger than 0. 997. The detection limits of dopamine and quercetin are 1. 0× 10~(-8)mol·L~(-1)and 1. 0× 10~(-6)mol·L~(-1),respectively.This method hold many merits: good reproducibility,high stability,and peak current of RSD = 1. 51%. We believe that the developed method can be applied for the detection of dopamine and quercetin in medicines.
A molecularly electrochemical sensor( N-CTS/MWNTs/GCE) was prepared using N-acyl chitosan( N-CTS) and multi walled carbon nanotubes( MWNTs) modified glassy carbon electrode. Cyclic voltammetry was applied to study the electrochemical behaviors of dopamine( DA) and quercetin( QU) on the modified N-CTS/MWNTs/GCE electrode Results showed that N-CTS/MWNTs/GCE could greatly improve the oxidation peak currents of both DA and QU.Linear response relationship between the peak current and the concentration of analytes could be established for dopamine and quercetin,as respectively measured in pH = 7. 38,6. 80 phosphate buffer solution.Linear equations can be described as followed: Ip( DA)= 0. 0397+ 4715. 8673 c and Ip( QU)=-0. 2645+256. 8935 c.The correlation coefficients for these two equations are all larger than 0. 997. The detection limits of dopamine and quercetin are 1. 0× 10~(-8)mol·L~(-1)and 1. 0× 10~(-6)mol·L~(-1),respectively.This method hold many merits: good reproducibility,high stability,and peak current of RSD = 1. 51%. We believe that the developed method can be applied for the detection of dopamine and quercetin in medicines.
引文
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[16]罗菊香,吴萍,俞小云,等.N-琥珀酰壳聚糖的制备及其溶解性研究[J].食品科技,2014,39(1):255-258.
[17]孙小龙,韩策,赵雯.6-羟基烟酸修饰电极的制备及应用研究[J].化学研究与应用,2016,28(8):1080-1085.
[2]杨银,张娜,汪丽,等.槲皮素在聚L-络氨酸修饰电极上的电化学行为及测定[J].中国无机分析化学,2016,6(1):77-82.
[3]张旭红,周清,翟海云,等.槲皮素在分子印迹聚合物修饰碳糊电极上的电化学行为及测定[J].中国测试,2015,41(11):50-54.
[4]顾玲,闫璟,柯苗.聚茜素红膜修饰碳糊电极对槲皮素的电催化氧化研究[J].化学研究与应用,2016,28(8):1086-1091.
[5]李素娟,邓德华,王贵芳,等.纳米金/石墨烯修饰电极的制备及其对多巴胺的测定[J].分析实验室,2012,31(3):14-17.
[6]郭新美,王宗花,夏建飞,等.多巴胺在聚亚甲基蓝/石墨烯修饰电极上的电化学行为研究[J].分析测试学报,2004,31(4):464-469.
[7]牛凌梅,连靠奇,马莉,等.双层纳米金修饰电极对多巴胺的测定及应用研究[J].化学研究与应用,2015,27(10):1514-1517.
[8].陈苏靖,李芳芳,钟云秀,等.基于聚溴酚蓝-石墨烯复合膜修饰电极的多巴胺电化学传感器[J].分析测试学报,2015,34(9):1066-1071.
[9]尹辉.槲皮素在氧化锆I石墨烯修饰玻碳电极上的电化学行为及检测[J].分析试验室,2015,34(9):1083-1085.
[10]李燕红,陈宗保,董洪霞,等.唑类有机化合物修饰电极同时测定尿液中的多巴胺和黄嘌呤[J],化学研究与应用,2015,27(8):1123-1127.
[11]王志贤,王赤贞胤,胡效亚.化学修饰电极的制备及其药物分析应用的研究进展[J].化学传感器,2007,27(4):8-12.
[12]方渊,江奇,温琦,等.碳纳米管/聚苯胺复合材料修饰电极制备及应用研究进展[J].应用化工,2015,44(6):1117-1120.
[13]马曾燕,李将渊,向伟.聚吡咯/多壁碳纳米管修饰电极对多巴胺的测定[J].化学研究与应用,2008,20(12):1570-1575.
[14]Cosio M S,PellicanòA,Brunettia B,et al.A simple hydroxylated multi-walled carbon nanotubes modified glassy carbon electrode for rapid amperometric detection of bisphenol A.[J].Sens Actuators B:Chem,2017,246(7):673-679.
[15]Deng D L,Deng F,Tang B B,et al.Electrocatalytic reduction of low-concentration thiamphenicol and florfenicol in wastewater with multi-walled carbon nanotubes modified electrode[J].J Hazard Mate,2017,322(6):168-175.
[16]罗菊香,吴萍,俞小云,等.N-琥珀酰壳聚糖的制备及其溶解性研究[J].食品科技,2014,39(1):255-258.
[17]孙小龙,韩策,赵雯.6-羟基烟酸修饰电极的制备及应用研究[J].化学研究与应用,2016,28(8):1080-1085.