急性pH和碳酸盐碱度对缢蛏存活率、Na~+/K~+-ATPase活性及血淋巴吞噬能力的影响
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摘要
采用静态毒理学实验方法,分析了2种规格缢蛏(小规格SSc和大规格LSc)在pH和碳酸盐碱度(C_A)急性胁迫条件下的存活率,Na~+/K~+-ATPase(NKA)活性以及血淋巴的吞噬能力。结果显示,当C_A浓度为2.5 mmol/L、pH值为7.5～9.5时,2种规格缢蛏的存活率均接近100%;当pH值大于9.5时,2种规格缢蛏的存活率均显著下降。当C_A浓度为0～44.58mmol/L、pH值为9.0～10.0时,随着C_A浓度的上升,缢蛏的存活率明显下降;在pH值为9.5条件下,LSc的鳃组织NKA活性,随着C_A浓度的上升而升高,LSc血淋巴的吞噬能力随着C_A浓度的上升而下降。由此可见,缢蛏在高pH或高C_A下表现出较强的耐受性,但高pH和高C_A协同胁迫下对缢蛏的存活率具有较大的影响,研究结果为进一步探索缢蛏在盐碱地的养殖提供了一定的理论参考。
In this study, a static toxicology experiment was conducted to analyze the survival rate, Na~+/K~+-ATPase(NKA) activity and phagocytic ability of the hemolymph of Sinonovacula constricta of two different sizes(smallsize SSc and large-size LSc) under the acute stress of high pH and carbonate alkalinity(C_A). The results showed that when the C_A concentration was 2.5 mmol/L and the pH was 7.5-9.5, the survival rates of the two sizes were close to 100%.When the pH value was greater than 9.5, the survival rates of the two sizes were significantly decreased.When the concentration of C_A was 0-44.58 mmol/L and the pH value was 9.0-10.0, the survival rate of clams decreased with the increase of C_A concentration. Under the condition of pH 9.5, the NKA activity of LSc gill tissue increased with the increase of C_A concentration, and the phagocytic capacity of LSc haemolymph decreased with the increase of C_A concentration. It can be seen that S. constricta showed strong tolerance to high pH or C_A,but high pH and C_A had a great effect on the survival rate of S. constricta. The research results provide a theoretical reference for further exploration of breeding of S. constricta in saline-alkali soil.
In this study, a static toxicology experiment was conducted to analyze the survival rate, Na~+/K~+-ATPase(NKA) activity and phagocytic ability of the hemolymph of Sinonovacula constricta of two different sizes(smallsize SSc and large-size LSc) under the acute stress of high pH and carbonate alkalinity(C_A). The results showed that when the C_A concentration was 2.5 mmol/L and the pH was 7.5-9.5, the survival rates of the two sizes were close to 100%.When the pH value was greater than 9.5, the survival rates of the two sizes were significantly decreased.When the concentration of C_A was 0-44.58 mmol/L and the pH value was 9.0-10.0, the survival rate of clams decreased with the increase of C_A concentration. Under the condition of pH 9.5, the NKA activity of LSc gill tissue increased with the increase of C_A concentration, and the phagocytic capacity of LSc haemolymph decreased with the increase of C_A concentration. It can be seen that S. constricta showed strong tolerance to high pH or C_A,but high pH and C_A had a great effect on the survival rate of S. constricta. The research results provide a theoretical reference for further exploration of breeding of S. constricta in saline-alkali soil.
引文
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[10]贾恢先.中国西北内陆盐渍化防治与可持续农业的研究[J].西北植物学报,2003,23(6):1063-1068.Jia H X.Soil salinization control and sustainable agriculture in north-west endoland region of China[J].Acta Botanica Boreali-Occidentalia Sinica,2003,23(6):1063-1068(in Chinese).
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[12]王慧,耿隆坤,房文红,等.中国对虾往西北内陆咸水水域移植的生产性试养研究[J].海洋渔业,1997(1):9-12.Wang H,Geng L K,Fang W H,et al.Studies on the commercially experimental culture of penaeid shrimp,Peneaus chinensis,transplanted to the northwest inland salt waters[J].Marine Fishery,1997(1):9-12(in Chinese).
[13]王佳丽,黄贤金,钟太洋,等.盐碱地可持续利用研究综述[J].地理学报,2011,66(5):673-684.Wang J L,Huang X J,Zhong T Y,et al.Review on sustainable utilization of salt-affected land[J].Acta Geographica Sinica,2011,66(5):673-684(in Chinese).
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[15]Rijstenbil J W,Gerringa L J A.Interactions of algal ligands,metal complexation and availability,and cell responses of the diatom Ditylum brightwellii with a gradual increase in copper[J].Aquatic Toxicology,2002,56(2):115-131.
[16]Partridge G J.Inland saline aquaculture:overcoming biological and technical constraints towards the development of an industry[D].Murdoch,WA:Murdoch University,2008.
[17]Van der Hiele T,Rijstenbil J W,Creemers J,et al.Composition,treatment and use of saline groundwater for aquaculture in the Netherlands saline groundwater composition and chemistry[J].World Aquaculture,2014:23-27.
[18]杨富亿,孙丽敏,杨欣乔.碳酸盐碱度对南美白对虾幼虾的毒性作用[J].水产科学,2004,23(9):3-6.Yang F Y,Sun L M,Yang X Q.Toxicity of carbonate alkalinity to Penaeus vannamei juveniles[J].Fisheries Science,2004,23(9):3-6(in Chinese).
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