吉富罗非鱼HSP70基因的多态性及其与耐寒性状的相关性研究
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摘要
利用PCR-SSCP技术对吉富罗非鱼HSP70基因多态性进行检测,并采用最小二乘法分析多态性与罗非鱼Oreochromis niloticus耐寒性状的相关性。结果发现,依据HSP70基因序列设计的引物HP70-1、HP70-4、HP70-6不同耐寒能力样本中的电泳图具有带型多态性,且该基因多态性与其耐寒性状显著相关。据此推断HSP70基因是潜在的罗非鱼耐寒相关基因。
The polymorphisms of HSP70 in the GIFT tilapia samples were detected by using PCR-SSCP technique and the correlation with cold tolerance capability of GIFT tilapia was analyzed by least square method in this study.The results showed that the electrophoresis diagram of the primer HP70-1,HP70-4,HP70-6 designed according to the HSP70 has the polymorphism of the belt type in different cold tolerance samples.The polymorphism of the gene was significantly correlated with its cold tolerance.Therefore,it is inferred that HSP70 genes are potential cold-resistant genes of tilapia.
The polymorphisms of HSP70 in the GIFT tilapia samples were detected by using PCR-SSCP technique and the correlation with cold tolerance capability of GIFT tilapia was analyzed by least square method in this study.The results showed that the electrophoresis diagram of the primer HP70-1,HP70-4,HP70-6 designed according to the HSP70 has the polymorphism of the belt type in different cold tolerance samples.The polymorphism of the gene was significantly correlated with its cold tolerance.Therefore,it is inferred that HSP70 genes are potential cold-resistant genes of tilapia.
引文
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[15]朱华平,卢迈新,黄樟翰,等.低温对罗非鱼基因组DNA甲基化的影响[J].水产学报,2013,37(10):1460-1467.
[2]马旦梅,程光平,喻海燕.吉富罗非鱼对低温持续胁迫的死亡反应[J].广西农业科学,2010,41(7):726-728.
[3]孔杰,王金乐,关梅,等.分子标记在鱼类中的应用研究进展[J].贵州农业科学,2012,40(6):158-162.
[4]顾真庆,冯志新,王占伟,等.PCR-SSCP技术在微生物检测中的应用[J].生物技术通报,2011(2):70-74.
[5] NOUMI T,MOSHER M E,NATORI S,et al.A phenylalanine for serine substitution in theβsubunit of Escherichia coli F1-ATPase affects dependence of its activity on divalent cations[J].The Journal of Biological Chemistry,1984,259(16):10071-10075.
[6] ZHOU H,CHENG L,GONG H,et al.Variation in the Toll-like Receptor 4(TLR4)gene affects milk traits in dairy cows[J].Journal of Dairy Research,2017,84(4):426-429.
[7] ZHANG X,WANG J,LIU H,et al.The clinicopathologic relevance of RECK gene polymorphisms in ameloblastoma[J].Archives of Oral Biology,2017,79:77-86.DOI:10.1016/j.archoralbio.2017.03.008.
[8] GUO X F,HU W P,LANG X Z,et al.Two single nucleotide polymorphisms sites inα1-ATgene and their association with somatic cell score in Chinese Holstein cows[J].Journal of Biological Research,2017,24(1):8.
[9] TAKLE H,BAEVERFJORD G,LUNDE M,et al.The effect of heat and cold exposure on HSP70expression and development of deformities during embryogenesis of Atlantic salmon(Salmo salar)[J].Aquaculture,2005,249(1):515-524.
[10]刘波,王美垚,谢骏,等.低温应激对吉富罗非鱼血清生化指标及肝脏HSP70基因表达的影响[J].生态学报,2011,31(17):4866-4873.
[11]李莉萍,陈明,唐章生,等.5个品系罗非鱼热休克蛋白Hsp70基因序列比对分析[J].西南农业学报,2009(3):828-833.
[12]王瑞,黄艳华,陈明,等.罗非鱼感染链球菌后hsp70基因表达研究[J].广西农业科学,2010,41(4):371-374.
[13] CNAANI A,HALLERMAN E M,RON M,et al.Detection of a chromosomal region with two quantitative trait loci,affecting cold tolerance and fish size,in an F2tilapia hybrid[J].Aquaculture,2003,223(1/2/3/4):117-128.
[14] MOEN T,AGRESTI J J,CNAANI A,et al.A genome scan of a four-way tilapia cross supports the existence of a quantitative trait locus for cold tolerance on linkage group 23[J].Aquaculture Research,2004,35(9):893-904.
[15]朱华平,卢迈新,黄樟翰,等.低温对罗非鱼基因组DNA甲基化的影响[J].水产学报,2013,37(10):1460-1467.