焦炉工GSTM1和GSTT1基因多态性与尿1-羟基芘浓度的关系研究
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摘要
目的探讨焦炉工GSTM1和GSTT1基因多态性与多环芳烃类(PAHs)代谢为1-羟基芘(1-OHP)过程之间的关系。方法以某焦化厂焦炉车间生产工人150人和其他车间无职业性PAHs接触者39人为调查对象。用高效液相色谱法测定尿中1-OHP浓度;聚合酶链反应一限制性片段长度多态性(PCRRFLP)法分别检测GSTM1和GSTT1基因多态性。采用方差分析和多因素回归分析等统计学方法研究影响尿中1-OHP浓度的因素。结果尿中1-OHP浓度与焦炉工作业的位置呈正相关(P<0.01)。低暴露组GSTT1(阴性)个体尿中1-OHP浓度较GSTT1(阳性)个体高;GSTM1(阴性)/GSTT1(阴性)全缺失型个体尿中1-OHP浓度明显高于GSTM1(阳性)/GSTT1(阳性)全野生型个体(P<0.05)。结论在PAHs低暴露水平下,GSTT1可以影响PAHs代谢。GSTM1和GSTT1基因的交互作用可以影响焦炉工PAHs代谢为1-OHP的过程。
Objective To explore the relationship between the GSTM1、GSTT1 gene polymorphism and the process of the PAHs metabolize into 1-OHP. Methods 150 workers working in the coke-oven plants and 39 workers working in the other plants without PAHs exposure were recruited. High performed liquid chromatography(HPLC)methods were used to measure 1-OHP concentration of the urine. PCR-RFLP method were used to detect the GSTM1、 GSTT1 gene polymorphism. ANOVA and multiple factor regression analysis were used to explore the factors affecting the urinary concentration of 1-OHP. Results Urinary concentration of 1-OHP were positive related with the working position in the coke-oven. 1-OHP concentration of urine of GSTT1(negative)individuals were higher than GSTT1(positive)individuals in the low exposure group. 1-OHP concentration of urine of GSTT1(negative)/GSTM1(negative)individuals were higher than GSTT1(positive)/GSTM1(positive) individuals. Conclusions Under low exposure of the PAHs, GSTT1 polymorphism can affect the PAHs metabolism. GSTM1、 GSTT1 gene interaction can affect the process of PAHs metabolic into1-OHP.
Objective To explore the relationship between the GSTM1、GSTT1 gene polymorphism and the process of the PAHs metabolize into 1-OHP. Methods 150 workers working in the coke-oven plants and 39 workers working in the other plants without PAHs exposure were recruited. High performed liquid chromatography(HPLC)methods were used to measure 1-OHP concentration of the urine. PCR-RFLP method were used to detect the GSTM1、 GSTT1 gene polymorphism. ANOVA and multiple factor regression analysis were used to explore the factors affecting the urinary concentration of 1-OHP. Results Urinary concentration of 1-OHP were positive related with the working position in the coke-oven. 1-OHP concentration of urine of GSTT1(negative)individuals were higher than GSTT1(positive)individuals in the low exposure group. 1-OHP concentration of urine of GSTT1(negative)/GSTM1(negative)individuals were higher than GSTT1(positive)/GSTM1(positive) individuals. Conclusions Under low exposure of the PAHs, GSTT1 polymorphism can affect the PAHs metabolism. GSTM1、 GSTT1 gene interaction can affect the process of PAHs metabolic into1-OHP.
引文
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[12]Yang MH,Jang JY,Kim SY,et al.Genetic effects on urinary 1-hydroxypyrene levels in a Korean population[J]Carcinogenesis,2003,24(6):1085-1089.
[2]Albrecht S,Anne S,Kurt Straif,et al.New biomarkers of occupational exposure to polycyclic aromatic hydrocarbons[J].J Toxicol Environ Health Part A,2008,71:734-745.
[3]胡正兴,成志,高华北.焦炉厂工人癌症流行病学调查[J].中国热带医学,2007,7(12):2346-2347.
[4]Ping B,Shuguang L,Juan C,et al.Association of aryl hydrocarbon receptor gene polymorphisms and urinary 1-hydroxypyrene in polycyclic aromatic hydrocarbon-exposed workers[J].Cancer Epidemiol Biomarkers Prev,2008,17(7):1702-1707.
[5]冷曙光,郑玉新,李晓华,等.焦炉工外暴露等级与尿中1-羟基芘水平的关系[J].工业卫生与职业病,2003,29(5):288-291.
[6]李晓华,冷曙光,郭君,等.改良的高效液相色谱法测定尿1-羟基芘[J].卫生研究,2003,32(6):616-617.
[7]中华人民共和国卫生部.WS/T97-1996尿中肌酐分光光度测定方法[S].北京:中国标准出版社,1997.
[8]常君瑞,徐东群,张文杰,等.环境外暴露及GSTT1、GSTM1基因型对1-羟基芘作为多环芳烃暴露标志物的影响[J].卫生研究,2009,38(3):261-264.
[9]Lee KH,Cho SH,Hong YC.el al.Urinary PAH metabolites influenced by genetic polymorphisms of GST Ml in male hospital incinerator workers[J].J Occup Health,2003,45(3):168-171.
[10]Leng SG,Zheng YX,Huang CF,et al.Effect of genetic polymorphisms of microsomal epoxide hydrolase on urinary 1-hydroxypyrene levels in coke oven workers[J].Zhonghua Laodongweisheng Zhiyebing Zazhi,2004,22(4):245-249.
[11]Apostoli P,Neri G.Influence of genetic polymorphisms of CYP1A1 and GSTM1 on the urinary levels of 1-hydroxypyrene.[J]Toxicol Lett,2003,144(1):2734.
[12]Yang MH,Jang JY,Kim SY,et al.Genetic effects on urinary 1-hydroxypyrene levels in a Korean population[J]Carcinogenesis,2003,24(6):1085-1089.