NK细胞对激素敏感型肾病综合征患儿预后判断的价值
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摘要
为探讨NK细胞在肾病综合征患儿中的临床价值,选取激素敏感型原发性肾病综合征(steroid-sensitive nephrotic syndrome, SSNS)患儿共63例,对照组30例,采用流式细胞术检测患儿治疗前后淋巴细胞亚群(CD4~+、CD8~+、CD4~+/CD8~+、CD19~+)和NK细胞,并在患儿出院后对其进行随访。结果显示治疗后患儿NK细胞水平显著升高(P<0.05),NK细胞水平与患儿预后显著相关。提示激素治疗能够显著影响SSNS患儿体内NK细胞的水平, NK细胞可以作为一个评估SSNS患儿预后的指标。
To evaluate the clinical significance of NK cells in children with steroid-sensitive nephrotic syndrome(SSNS), and evaluate the clinical significance of NK cells, a total of 63 children with SSNS and 30 controls were collected. Lymphocyte subsets and NK cells were measured by flow cytometry. The results showed that the percentage of NK cells increased significantly after treatment(P < 0.05), and NK cell levels were closely related to prognosis of the patients. The percentage of NK cells in children with SSNS was affected by glucocorticoid, and it could be an indicator for the prognosis of children with SSNS.
To evaluate the clinical significance of NK cells in children with steroid-sensitive nephrotic syndrome(SSNS), and evaluate the clinical significance of NK cells, a total of 63 children with SSNS and 30 controls were collected. Lymphocyte subsets and NK cells were measured by flow cytometry. The results showed that the percentage of NK cells increased significantly after treatment(P < 0.05), and NK cell levels were closely related to prognosis of the patients. The percentage of NK cells in children with SSNS was affected by glucocorticoid, and it could be an indicator for the prognosis of children with SSNS.
引文
[1] Uwaezuoke SN. The role of novel biomarkers in childhood idiopathic nephrotic syndrome: A narrative review of published evidence[J]. Int J Nephrol Renovasc Dis, 2017, 10: 123-128.
[2] 中华医学会儿科学分会肾脏病学组. 小儿肾小球疾病的临床分类、诊断及治疗[J]. 中华儿科杂志, 2001, 39(12): 746.
[3] Colucci M, Corpetti G, Emma F, et al. Immunology of idiopathic nephrotic syndrome[J]. Pediatr Nephrol, 2018, 33(4): 573-584.
[4] 张静, 李喆, 党西强. 咪唑立宾治疗儿童频复发型原发肾病综合征45例疗效观察[J]. 中国实用儿科杂志, 2017, 32(2): 149-152.
[5] Couderc A, Bérard E, Guigonis V, et al. Treatments of steroid-dependent nephrotic syndrome in children[J]. Arch Pediatr, 2017, 24(12): 1312-1320.
[6] Malmberg KJ, Carlsten M, Bj?rklund A, et al. Natural killer cell-mediated immunosurveillance of human cancer[J]. Semin Immunol, 2017, 31: 20-29.
[7] Tu MM, Mahmoud AB, Makrigiannis AP. Licensed and unlicensed NK cells:Differential roles in cancer and viral control[J]. Front Immunol, 2016, 7: 166.
[8] 刘艾芹, 于磊, 李晖云, 等. 原发性肾病综合征患者外周血淋巴细胞亚群变化及临床意义[J]. 河北医学, 2014, 20(3): 458-460.
[9] 刘峻峰. 学龄前原发性肾病综合征患儿淋巴细胞亚群测定[J]. 郑州大学学报(医学版), 2013, 33(4): 547-550.
[10] Makita S, Tobinai K. Antibody therapy targeting CD19 for B-cell non-Hodgkin lymphoma[J]. Ann Oncol, 2018, 29(5): 1086-1089.
[11] 柳梅, 任维鑫, 任红艳. 甲泼尼龙琥珀酸钠早期治疗手足口病疗效观察[J]. 中国医药导刊, 2014, 16(4): 669-670.
[12] Gianchecchi E, Delfino DV, Fierabracci A. NK cells in autoimmune diseases: Linking innate and adaptive immune responses[J]. Autoimmun Rev, 2018, 17(2): 142-154.
[13] Ge X, Li CR, Yang J, et al. Aberrantly decreased levels of NKG2D expression in children with kawasaki disease[J]. Scand J Immunol, 2013, 77(55): 389-397.
[14] Souza-Fonseca-Guimaraes F, Cavaillon JM, Adib-Conquy M. Bench-to-bedside review: Natural killer cells in sepsis-guilty or not guilty?[J]. Crit Care, 2013, 17(4): 235.
[15] 安丽欣, 刘爱学. IL-23及其受体与类风湿关节炎疾病活动的相关性分析[J]. 现代免疫学, 2018, 38(4): 321-324.
[16] 郑荣浩, 朱光华, 王平, 等. 原发性肾病综合征患儿外周血Zeta链改变临床意义分析[J]. 中国实用儿科杂志, 2011, 26(6): 439-442.
[2] 中华医学会儿科学分会肾脏病学组. 小儿肾小球疾病的临床分类、诊断及治疗[J]. 中华儿科杂志, 2001, 39(12): 746.
[3] Colucci M, Corpetti G, Emma F, et al. Immunology of idiopathic nephrotic syndrome[J]. Pediatr Nephrol, 2018, 33(4): 573-584.
[4] 张静, 李喆, 党西强. 咪唑立宾治疗儿童频复发型原发肾病综合征45例疗效观察[J]. 中国实用儿科杂志, 2017, 32(2): 149-152.
[5] Couderc A, Bérard E, Guigonis V, et al. Treatments of steroid-dependent nephrotic syndrome in children[J]. Arch Pediatr, 2017, 24(12): 1312-1320.
[6] Malmberg KJ, Carlsten M, Bj?rklund A, et al. Natural killer cell-mediated immunosurveillance of human cancer[J]. Semin Immunol, 2017, 31: 20-29.
[7] Tu MM, Mahmoud AB, Makrigiannis AP. Licensed and unlicensed NK cells:Differential roles in cancer and viral control[J]. Front Immunol, 2016, 7: 166.
[8] 刘艾芹, 于磊, 李晖云, 等. 原发性肾病综合征患者外周血淋巴细胞亚群变化及临床意义[J]. 河北医学, 2014, 20(3): 458-460.
[9] 刘峻峰. 学龄前原发性肾病综合征患儿淋巴细胞亚群测定[J]. 郑州大学学报(医学版), 2013, 33(4): 547-550.
[10] Makita S, Tobinai K. Antibody therapy targeting CD19 for B-cell non-Hodgkin lymphoma[J]. Ann Oncol, 2018, 29(5): 1086-1089.
[11] 柳梅, 任维鑫, 任红艳. 甲泼尼龙琥珀酸钠早期治疗手足口病疗效观察[J]. 中国医药导刊, 2014, 16(4): 669-670.
[12] Gianchecchi E, Delfino DV, Fierabracci A. NK cells in autoimmune diseases: Linking innate and adaptive immune responses[J]. Autoimmun Rev, 2018, 17(2): 142-154.
[13] Ge X, Li CR, Yang J, et al. Aberrantly decreased levels of NKG2D expression in children with kawasaki disease[J]. Scand J Immunol, 2013, 77(55): 389-397.
[14] Souza-Fonseca-Guimaraes F, Cavaillon JM, Adib-Conquy M. Bench-to-bedside review: Natural killer cells in sepsis-guilty or not guilty?[J]. Crit Care, 2013, 17(4): 235.
[15] 安丽欣, 刘爱学. IL-23及其受体与类风湿关节炎疾病活动的相关性分析[J]. 现代免疫学, 2018, 38(4): 321-324.
[16] 郑荣浩, 朱光华, 王平, 等. 原发性肾病综合征患儿外周血Zeta链改变临床意义分析[J]. 中国实用儿科杂志, 2011, 26(6): 439-442.