柔肝通络汤联合依达拉奉对脑梗死模型大鼠脑可塑性物质及结构影响作用
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摘要
目的:分析柔肝通络汤联合依达拉奉对脑梗死模型大鼠脑可塑性物质及结构影响。方法:选取SPF级Wistar雄性大鼠60只,随机数字表法将大鼠分成三组,正常组、模型组与观察组,每组各20只。除正常组其他各组大鼠制备脑梗死模型,给药后7天随机选取8只大鼠,给药后21天选取剩余大鼠,断颈处死后选取脑组织内缺血中心位置冠状脑片,行HE染色,免疫检测大鼠冠状脑片内突触素(SY38)及神经微丝蛋白(NFP-200)表达状况。结果:给药7天、21天时模型组大鼠SY38、NFP-200表达量比正常组下降,观察组比模型组上升,差异均有统计学意义(P<0.05)。结论:依达拉奉结合柔肝通络汤可使脑梗死大鼠脑组织内NFP-200、SY38表达量升高,降低神经细胞和毛细血管缺血性受损,加速突出超微结构功能变强。
Objective:To analyze the effect of Rougong Tongluo Decoction combined with Edaravone on brain plasticity and structure for rats with cerebral infarction.Methods:Sixty Wistar male rats of SPF grade were randomly divided into three groups:normal group,model group and observation group,with 20 rats in each group.In addition to the rats in the normal group,the cerebral infarction model was prepared.Eight rats were randomly selected 7 days after the administration.The remaining rats were selected 21 days after the administration.After the neck was sacrificed,the ischemic center position of the brain was selected.HE staining was performed to detect the expression of synaptophysin(SY38) and neurofilament protein(NFP-200) in rat coronary slices.Results:The expression levels of SY38 and NFP-200 in the model group were lower than those in the normal group at 7 days and 21 days after administration.The observation group was higher than the model group,and the difference was statistically significant(P<0.05).Conclusion:Edaravone combined with Rougan Tongluo Decoction can increase the expression of NFP-200 and SY38 in brain tissue of rats with cerebral infarction,reduce the ischemic damage of nerve cells and capillaries,and accelerate the prominent ultrastructural function to become strong.
Objective:To analyze the effect of Rougong Tongluo Decoction combined with Edaravone on brain plasticity and structure for rats with cerebral infarction.Methods:Sixty Wistar male rats of SPF grade were randomly divided into three groups:normal group,model group and observation group,with 20 rats in each group.In addition to the rats in the normal group,the cerebral infarction model was prepared.Eight rats were randomly selected 7 days after the administration.The remaining rats were selected 21 days after the administration.After the neck was sacrificed,the ischemic center position of the brain was selected.HE staining was performed to detect the expression of synaptophysin(SY38) and neurofilament protein(NFP-200) in rat coronary slices.Results:The expression levels of SY38 and NFP-200 in the model group were lower than those in the normal group at 7 days and 21 days after administration.The observation group was higher than the model group,and the difference was statistically significant(P<0.05).Conclusion:Edaravone combined with Rougan Tongluo Decoction can increase the expression of NFP-200 and SY38 in brain tissue of rats with cerebral infarction,reduce the ischemic damage of nerve cells and capillaries,and accelerate the prominent ultrastructural function to become strong.
引文
[1] 宋长明,黄佳,林冰冰,等.电针百会、神庭穴对脑缺血再灌注大鼠学习记忆能力及海马CA1区突触超微结构的影响[J].中国康复理论与实践,2017,23(7):750～755
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[11] 高珊,孔立红,余超超,等.不同频率电针通过调控GSK-3β蛋白及其磷酸化水平改善阿尔茨海默病大鼠突触可塑性损伤[J].广州中医药大学学报,2017,34(5):696～702
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[15] 龙飞,李学智,龚标,等.电针对局灶性脑梗死大鼠Slit2及SrGAP1表达的影响[J].第二军医大学学报,2016,37(1):46～51
[16] 苗培芳,程敬亮,王彩鸿,等.基于体素的形态学分析研究皮层下脑梗死患者脑结构的改变[J].实用放射学杂志,2016,32(6):841～844
[2] Seule M,Sikorski C,Sakowitz O,et al.Evaluation of a New Brain Tissue Probe for Intracranial Pressure,Temperature,and Cerebral Blood Flow Monitoring in Patients with Aneurysmal Subarachnoid Hemorrhage.[J].Neurocritical Care,2016,25(2):1～8
[3] 马丙祥,王怡珍,党伟利,等.丹参注射液对缺氧缺血脑损伤新生大鼠突触重塑的影响[J].中华实用儿科临床杂志,2016,31(14):1086～1090
[4] 刘斐雯,俞坤强,彭洪卫,等.电针百会、神庭穴对局灶性脑缺血再灌注大鼠学习记忆能力及海马区GAP-43蛋白表达的影响[J].中国中医急症,2016,25(2):189～192
[5] 王路娜,陈一冰,张均,等.可卡因-苯丙胺调节转录肽对缺血-再灌注模型小鼠皮质突触可塑性的影响[J].中国脑血管病杂志,2017,14(3):127～132
[6] Westermaier T,Stetter C,Kunze E,et al.Controlled Hypercapnia Enhances Cerebral Blood Flow and Brain Tissue Oxygenation After Aneurysmal Subarachnoid Hemorrhage:Results of a Phase 1Study[J].Neurocritical Care,2016,25(2):1～10
[7] 谷宁,季亚清,张睿,等.神经调节素对脑缺血再灌注损伤后神经组织形态结构的影响[J].中华全科医学,2016,14(9):1453～1456
[8] 宋江华,李刚,元小冬,等.小鼠大脑中动脉缺血再灌注后缺血区及周边区星形胶质细胞的变化[J].中国神经免疫学和神经病学杂志,2017,24(3):201～205
[9] 江飞飞,林海燕,丁美萍.急性脑梗死患者血小板超微结构及相关参数变化的研究[J].浙江医学,2016,38(2):126～127
[10] Gu N,Ge K,Cui H,et al.Neuregulin1β Effects on Brain Tissue via ERK5-Dependent MAPK Pathway in a Rat Model of Cerebral Ischemia–Reperfusion Injury[J].Journal of Molecular Neuroscience,2017,61(4):607～616
[11] 高珊,孔立红,余超超,等.不同频率电针通过调控GSK-3β蛋白及其磷酸化水平改善阿尔茨海默病大鼠突触可塑性损伤[J].广州中医药大学学报,2017,34(5):696～702
[12] Zhang Z Q,Song J Y,Jia Y Q,et al.Buyang Huanwu Decoction promotes angiogenesis after cerebral ischemia/reperfusion injury:mechanisms of brain tissue repair[J].Neural Regeneration Research,2016,11(3):435～440
[13] 张婷婷,张树峰,滕尧,等.芪蛭胶囊对缺血性中风大鼠脑组织超微结构的影响[J].中医药信息,2016,33(1):22～24
[14] Harada S,Tokuyama S.Involvement of communication system between brain and peripheral tissues on the development of post-ischemic glucose intolerance induced by cerebral neuronal damage[J].Nihon Yakurigaku Zasshi,2016,142(1):34～38
[15] 龙飞,李学智,龚标,等.电针对局灶性脑梗死大鼠Slit2及SrGAP1表达的影响[J].第二军医大学学报,2016,37(1):46～51
[16] 苗培芳,程敬亮,王彩鸿,等.基于体素的形态学分析研究皮层下脑梗死患者脑结构的改变[J].实用放射学杂志,2016,32(6):841～844
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