芽孢杆菌污染导致绝缘油油质劣化的试验研究
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摘要
为解决杂菌污染导致绝缘油油质劣化的问题,对分离杂菌进行鉴定,考察培养条件及其对介质损耗、pH、微水含量和表面张力的影响,从而提出解决方法。采用唯一碳源培养基分离杂菌并借助生化试验、16sDNA技术鉴定种属。通过吸光度的变化探讨杂菌适宜生存温度并在此条件下对上述4种指标进行70 d的检测。结果表明:成功分离1种芽孢杆菌,在绝缘油中最适生长温度为37℃。该菌能够导致绝缘油介质损耗变大且导致油质环境酸化。绝缘材料的使用能够维持介质损耗在合格范围内,并一定程度抑制杂菌生长。绝缘油的表面张力变化与杂菌和绝缘材料不存在明显关系。芽孢杆菌可在绝缘油中存活且最适生长温度为37℃,并导致油质劣化。
To solve the problem of insulating oil quality deterioration caused by bacterial contamination, we indentified the infectious microbes existing in the inferior insulating oil and confirmed the optimum growth temperature. Essentially, based on the effects of bacteria on dielectric loss, pH, moisture content and surface tension, we put forward some solutions to this problem. Solo carbon source was performed to screen out the infectious microbes and its species were indentified by biochemical experiments combining with 16 sDNA technology. The optimum growth temperature was confirmed based on the results of absorbance value. Meanwhile, a 70-day electrical test was designed to check the function of bacteria on four indexes of insulating oil. Bacillus was successfully indentified and the optimum growth temperature was 37 ℃ in insulating oil. Both the increase of dielectric loss and acidification of oil environment were determined in insulating oil infected by bacillus. However, the insulating media were employed to keep the dielectric loss in a standard range and partly inhibit the growth of bacillus. Besides, surface tension and bacillus or insulating media did not show obvious relations in the experiment. Insulating oil can be infected by bacillus and the optimum temperature of growth is 37 ℃, leading to the deterioration of oil.
To solve the problem of insulating oil quality deterioration caused by bacterial contamination, we indentified the infectious microbes existing in the inferior insulating oil and confirmed the optimum growth temperature. Essentially, based on the effects of bacteria on dielectric loss, pH, moisture content and surface tension, we put forward some solutions to this problem. Solo carbon source was performed to screen out the infectious microbes and its species were indentified by biochemical experiments combining with 16 sDNA technology. The optimum growth temperature was confirmed based on the results of absorbance value. Meanwhile, a 70-day electrical test was designed to check the function of bacteria on four indexes of insulating oil. Bacillus was successfully indentified and the optimum growth temperature was 37 ℃ in insulating oil. Both the increase of dielectric loss and acidification of oil environment were determined in insulating oil infected by bacillus. However, the insulating media were employed to keep the dielectric loss in a standard range and partly inhibit the growth of bacillus. Besides, surface tension and bacillus or insulating media did not show obvious relations in the experiment. Insulating oil can be infected by bacillus and the optimum temperature of growth is 37 ℃, leading to the deterioration of oil.
引文
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[5] Toledo F L, Calvo C, Rodelas B, et al. Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities[J]. Systematic and Applied Microbiology,2006, 29 (3):244-252.
[6] 李兴.绝缘油的净化处理技术[J].清洗世界,2016,11(32):36-38.
[7] 韦强,肖福明,胡秉海,等.变压器油中微生物的浅析[J].变压器,2001,38(3):21-24.
[8] 阎雪梅,韦晨,赵婕,等.绝缘油介质中水分含量对其电气性能的影响[J].绝缘材料,2016,49(7):79-82.
[9] 马卫平,李绍英,程芳晓,等.微生物对大型变压器油介损的影响[J].吉林电力,2001(3):4-6.
[10] 邬家义.绝缘油存储期间的微生物污染问题[J].变压器,1995(4):18-20.
[11] 姬晓川,李庆玲,梁志钰.变压器油受微生物污染研究[J].变压器,2007,44(2):43-46.
[2] 刘贤忠,姚丽丽,朴真三,等. 大型变压器介质油细菌的研究[J].吉林大学自然科学学报, 1999(4): 5-8.
[3] 姚丽丽,梅子青,刘贤忠,等. 变压器介质油中枝动菌和罗丝氏菌的生化性质及培养条件[J]. 吉林大学自然科学学报, 2000(2):93-96.
[4] 布坎南RE,吉布斯NE.伯杰氏细菌鉴定手册 (第8版)[M].北京:科学出版社, 1984: 741-747.
[5] Toledo F L, Calvo C, Rodelas B, et al. Selection and identification of bacteria isolated from waste crude oil with polycyclic aromatic hydrocarbons removal capacities[J]. Systematic and Applied Microbiology,2006, 29 (3):244-252.
[6] 李兴.绝缘油的净化处理技术[J].清洗世界,2016,11(32):36-38.
[7] 韦强,肖福明,胡秉海,等.变压器油中微生物的浅析[J].变压器,2001,38(3):21-24.
[8] 阎雪梅,韦晨,赵婕,等.绝缘油介质中水分含量对其电气性能的影响[J].绝缘材料,2016,49(7):79-82.
[9] 马卫平,李绍英,程芳晓,等.微生物对大型变压器油介损的影响[J].吉林电力,2001(3):4-6.
[10] 邬家义.绝缘油存储期间的微生物污染问题[J].变压器,1995(4):18-20.
[11] 姬晓川,李庆玲,梁志钰.变压器油受微生物污染研究[J].变压器,2007,44(2):43-46.