山桐子油对力竭运动大鼠心肌线粒体的保护作用
|
摘要
为了探讨山桐子油对力竭运动大鼠心肌线粒体的保护作用,将50只SD大鼠随机分成对照组,力竭运动组,低、中、高剂量山桐子油组,其中对照组不进行任何运动,力竭运动组进行跑步运动训练,低、中、高剂量山桐子油组进行跑步运动的同时在运动之后2 h分别灌胃山桐子油,实验持续6周,每周6日,周日休息。研究结果表明:力竭运动组大鼠心肌线粒体内的ATP合成能力、SOD活性、GSH-Px活性、CAT活性与对照组相比均明显降低(P <0. 05,P <0. 01),Ca~(2+)含量、MDA含量与对照组相比明显增加(P <0. 05,P <0. 01);低、中、高剂量山桐子油组大鼠的ATP合成能力、SOD活性、GSH-Px活性、CAT活性呈上升趋势,Ca~(2+)含量、MDA含量呈下降趋势;高剂量山桐子油组大鼠的ATP合成能力、SOD活性、GSH-Px活性、CAT活性、Ca~(2+)含量、MDA含量与力竭运动组相比具有显著性差异(P <0. 05,P <0. 01)。说明山桐子油对力竭运动大鼠心肌线粒体有很好的保护作用。
In order to explore the protective effect of Idesia policarpa Maxim oil on myocardial mitochondria in rats after exhaustive exercise,fifty SD rats were randomly divided into control group,exhaustive exercise group,low,middle and high dose of Idesia policarpa Maxim oil groups. The control group did no exercise,and the exhaustive exercise group did run training. The low,middle and high dose groups were fed with Idesia policarpa Maxim oil 2 h after running. The training was performed for 6 weeks,6 d a week and rested on Sunday. The results showed that ATP synthesis ability,SOD activity,GSH-Px activity and CAT activity in myocardial mitochondria of exhaustive exercise group were significantly lower than those of control group (P < 0. 05,P < 0. 01),Ca~(2+) content and MDA content were significantly higher than those of control group (P < 0. 05,P < 0. 01). The ATP synthesis ability,SOD activity,GSH-Px activity and CAT activity of low,middle and high dose of Idesia policarpa Maxim oil groups increased,while Ca~(2+) content and MDA content decreased. The ATP synthesis ability,SOD activity,GSH-Px activity,CAT activity,Ca~(2+) content and MDA content of high dose of Idesia policarpa Maxim oil group had significant differences with the exhaustive exercise group (P < 0. 05,P < 0. 01). The results indicated that the Idesia policarpa Maxim oil had a good protective effect on myocardial mitochondria in rats after exhaustive exercise.
In order to explore the protective effect of Idesia policarpa Maxim oil on myocardial mitochondria in rats after exhaustive exercise,fifty SD rats were randomly divided into control group,exhaustive exercise group,low,middle and high dose of Idesia policarpa Maxim oil groups. The control group did no exercise,and the exhaustive exercise group did run training. The low,middle and high dose groups were fed with Idesia policarpa Maxim oil 2 h after running. The training was performed for 6 weeks,6 d a week and rested on Sunday. The results showed that ATP synthesis ability,SOD activity,GSH-Px activity and CAT activity in myocardial mitochondria of exhaustive exercise group were significantly lower than those of control group (P < 0. 05,P < 0. 01),Ca~(2+) content and MDA content were significantly higher than those of control group (P < 0. 05,P < 0. 01). The ATP synthesis ability,SOD activity,GSH-Px activity and CAT activity of low,middle and high dose of Idesia policarpa Maxim oil groups increased,while Ca~(2+) content and MDA content decreased. The ATP synthesis ability,SOD activity,GSH-Px activity,CAT activity,Ca~(2+) content and MDA content of high dose of Idesia policarpa Maxim oil group had significant differences with the exhaustive exercise group (P < 0. 05,P < 0. 01). The results indicated that the Idesia policarpa Maxim oil had a good protective effect on myocardial mitochondria in rats after exhaustive exercise.
引文
[1]包杰,陈凤香.不同产地山桐子果含油率和脂肪酸组成分析[J].粮食与油脂,2016,29(5):35-36.
[2]卢翔,李效文,郑坚,等.木本油料树种山桐子研究进展[J].农业科技通讯,2010(5):23-27.
[3]吴志文,谢双喜,刘青,等.山桐子的研究进展及应用前景[J].贵州农业科学,2010,38(1):161-164.
[4]莫开林,张正香,罗小龙,等.山桐子油的开发利用[J].粮油食品科技,2009,17(6):23-25.
[5]左蕾蕾.毛叶山桐子用于保健食品的可行性研究初探[J].轻工科技,2014(3):10-12.
[6]黄心敏,仇兆倩,王俊杰,等.山桐子油的研究进展[J].粮食与油脂,2017,30(4):11-13.
[7]吴发旺,刘春雷.响应面优化冷榨法制取山桐子油工艺的研究[J].食品工业科技,2012,33(11):278-282.
[8]井明星.维生素E油茶籽油不饱和脂肪酸酯合成及工艺研究[D].南宁:广西大学,2008.
[9]王文成,刘克敏.力竭运动对小鼠心肌线粒体自由基代谢和线粒体功能的影响[J].广州体育学院学报,2008,28(1):106-107.
[10]公雪,李晓燕.力竭运动对心肌组织结构及功能影响的研究进展[J].心血管病学进展,2016,37(4):435-440.
[11]华婉,叶扬,王战国,等.山桐子油的提取分离及理化性质研究[J].四川大学学报(自然科学版),2016,53(1):181-186.
[12]陈福北,陈少东.毛叶山桐子油中脂肪酸组成分析[J].化工技术与开发,2013,42(1):33.
[13]代娟,张小春,田艳萍,等.毛叶山桐子油抗氧化活性和成分分析[J].中国油脂,2016,41(8):70-73.
[14]张尧天,吕国枫,宫德正,等.急性运动对小鼠心肌线粒体功能的影响[J].中国康复,2006,21(6):366-367.
[15]王振富,钟灵,肖本见.富硒板党对力竭运动大鼠心肌线粒体抗氧化作用的影响[J].中国应用生理学杂志,2013,29(2):177-178.
[2]卢翔,李效文,郑坚,等.木本油料树种山桐子研究进展[J].农业科技通讯,2010(5):23-27.
[3]吴志文,谢双喜,刘青,等.山桐子的研究进展及应用前景[J].贵州农业科学,2010,38(1):161-164.
[4]莫开林,张正香,罗小龙,等.山桐子油的开发利用[J].粮油食品科技,2009,17(6):23-25.
[5]左蕾蕾.毛叶山桐子用于保健食品的可行性研究初探[J].轻工科技,2014(3):10-12.
[6]黄心敏,仇兆倩,王俊杰,等.山桐子油的研究进展[J].粮食与油脂,2017,30(4):11-13.
[7]吴发旺,刘春雷.响应面优化冷榨法制取山桐子油工艺的研究[J].食品工业科技,2012,33(11):278-282.
[8]井明星.维生素E油茶籽油不饱和脂肪酸酯合成及工艺研究[D].南宁:广西大学,2008.
[9]王文成,刘克敏.力竭运动对小鼠心肌线粒体自由基代谢和线粒体功能的影响[J].广州体育学院学报,2008,28(1):106-107.
[10]公雪,李晓燕.力竭运动对心肌组织结构及功能影响的研究进展[J].心血管病学进展,2016,37(4):435-440.
[11]华婉,叶扬,王战国,等.山桐子油的提取分离及理化性质研究[J].四川大学学报(自然科学版),2016,53(1):181-186.
[12]陈福北,陈少东.毛叶山桐子油中脂肪酸组成分析[J].化工技术与开发,2013,42(1):33.
[13]代娟,张小春,田艳萍,等.毛叶山桐子油抗氧化活性和成分分析[J].中国油脂,2016,41(8):70-73.
[14]张尧天,吕国枫,宫德正,等.急性运动对小鼠心肌线粒体功能的影响[J].中国康复,2006,21(6):366-367.
[15]王振富,钟灵,肖本见.富硒板党对力竭运动大鼠心肌线粒体抗氧化作用的影响[J].中国应用生理学杂志,2013,29(2):177-178.