新疆南疆某规模牛场轮状病毒基因分型鉴定与分析

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英文篇名：Identification and analysis of the genetype of bovine rotavirus in a large-scale cattle farm in southern Xinjiang 作者：张迎春 ; 崔鑫 ; 王廷龙 ; 朱广艺 ; 黄腾 ; 宋健颖 ; 胡建军 英文作者：ZHANG Yingchun;CUI Xin;WANG Tinglong;ZHU Guangyi;HUANG Teng;SONG Jianying;HU Jianjun;The Veterinary Station of the First Division, Xinjiang Production and Construction Group;College of Animal Science, Tarim University; 关键词：牛轮状病毒 ; RT-PCR ; 基因分型 英文关键词：bovine rotavirus;;RT-PCR;;genotype identification 中文刊名：XMYS 英文刊名：Animal Husbandry & Veterinary Medicine 机构：新疆生产建设兵团第一师畜牧兽医工作站;塔里木大学动物科学学院; 出版日期：2019-04-10 出版单位：畜牧与兽医 年：2019 期：v.51;No.405 基金：新疆生产建设兵团第一师科技局资助项目(2016XS03);新疆生产建设兵团科技局现代农业科技攻关与成果转化资助项目(2016AC012);; 国家自然科学基金(31660725) 语种：中文; 页：XMYS201904017 页数：5 CN：04 ISSN：32-1192/S 分类号：85-89

摘要

新疆南疆某规模奶牛场部分犊牛出生后1周左右出现严重腹泻,严重地影响了犊牛健康。为查明该牛场犊牛腹泻是否存在牛轮状病毒感染以及病毒基因类型,以及更好地防治犊牛腹泻发生。试验采集了部分腹泻犊牛的粪便样本,提取粪样病毒核酸、设计病毒特异性扩增引物,采用RT-PCR法、基因测序、核苷酸同源性分析及构建遗传进化树。结果显示,待检样本通过VP7特异性引物(VP7-1/VP7/2)和基因分型鉴定引物(VP7-1/G10-2)的RT-PCR扩增得到大小1 062 bp、715 bp目的片段,经测序及核苷酸同源性比对分析,确定该样本为轮状病毒A群G10型,结合临床诊断、病理变化确定该牛场疑似患牛存在牛轮状病毒感染,为该病防控提供了重要的理论依据。
        In a large-scale cattle farm in southern Xinjiang, some calves developed severe diarrhea, producing serious effect on their health. In order to determine whether the diarrhea was caused by bovine rotavirus and to determine the genotype of the virus, RNA samples were extracted and tested by RT-PCR with special primers, VP7-1/VP7/2 and VP7-1/G10-2. The result showed that the lengths of the VP7 gene and the G10 gene were 1 062 bp and 715 bp respectively. The samples were identified as genotype G10 of Group A of bovine rotavirus according to the genetic sequencing and gene sequence analysis in the study. The clinical diagnosis and the information of pathological changes proved that the diarrhea in the calves was caused by bovine rotavirus. The present study provided important theoretical reference for prevention and control of bovine rotavirus.

引文

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