红曲菌主要代谢产物检测方法研究进展
|
摘要
红曲菌能产生多种代谢产物,这些代谢产物大多具有一定的生理活性,而桔霉素是一种真菌毒素。文章对红曲菌主要代谢产物Monascolins K、红曲色素、麦角甾醇、γ-氨基丁酸和桔霉素的检测方法进行归纳总结,以期对红曲菌代谢产物的研究开发提供参考。
Monascus can produce many compounds,most of which have obvious biological activity.While citrinin is a mycotoxin.In this paper,the detection methods of major metabolites Monascolins K,Monascus pigment,ergosterol,γ-aminobutyric acid(GABA),citrinin are summarized.It can be used as a reference for related research and development of secondary metabolites from Monascus.
Monascus can produce many compounds,most of which have obvious biological activity.While citrinin is a mycotoxin.In this paper,the detection methods of major metabolites Monascolins K,Monascus pigment,ergosterol,γ-aminobutyric acid(GABA),citrinin are summarized.It can be used as a reference for related research and development of secondary metabolites from Monascus.
引文
[1]李钟庆,晓暾,郭芳.综观红曲与红曲菌[M].北京:中国轻工业出版社,2009.
[2]吴芳彤.高产洛伐他汀红曲菌的分离鉴定及其红曲发酵条件的优化[D].福州:福建农林大学,2014.
[3]马美荣,王正祥.红曲有效生理活性物质的研究现状与进展[J].酿酒科技,1999(5):26-27.
[4]魏巍.红曲霉发酵合成洛伐他汀的研究[D].武汉:华中科技大学,2013.
[5]曹纪亮,孔维军,杨美华,等.真菌毒素快速检测方法研究进展[J].药物分析杂志,2013(1):159-164.
[6]杨丽,张水华,王启军.TLC薄层色谱法同步检测功能红曲中酸型和内酯型莫那呵啉K(Monacolin K)[J].食品研究与开发,2006,27(2):113-114.
[7]汤卫华,乔长晟,许春英.紫外分光光度法检测Monacolin K的含量[J].食品工业科技,2004,25(4):129-130.
[8]文镜,顾晓玲,常平等.双波长紫外分光光度法测定红曲中洛伐他汀的含量[J].中国食品添加剂,2000(4):11-17.
[9]张庆庆,危勤涛,汤斌,等.毛细管电泳法测定红曲胶囊中洛伐他汀含量[J].中国实验方剂学杂志,2008(11):4-6.
[10]Nigovic B,Sertic M,Mornar A.Simultaneous determination of lovastatin and citrinin in red yeast rice supplements by micellar electrokinetic capillary chromatography[J].Food Chem,2013,138(1):531-538.
[11]Friedrich J,uek M,Bencˇina M,et al.High-performance liquid chromatographic analysis of mevinolin as mevinolinic acid in fermentation broths[J].Journal of Chromatography A,1995,704(2):363-367.
[12]Su Y C,Wang J J,Lin T T,et al.Production of the secondary metabolites gamma-aminobutyric acid and monacolin K by Monascus[J].Journal of Industrial Microbiology&Biotechnology,2003(30):41-46.
[13]Lee C L,Wang J J,Pan T M.Synchronous analysis method for detection of citrinin and the lactone and acid forms of monacolin K in red mold rice[J].Journal of Aoac International,2006,89(3):669-677.
[14]Ajdari Z,Ebrahimpour A,Abdul M M,et al.Assessment of monacolin in the fermented products using Monascus purpureus FTC5391[J].Biomed Research International,2011(1):426168-426168.
[15]Song F,El-demerdash A,Lee S J S H,et al.Fast screening of lovastatin in red yeast rice products by flow injection tandem mass spectrometry[J].Journal of Pharmaceutical&Biomedical Analysis,2011,57(2):76-81.
[16]陈奇,王伟平,张华山,等.红曲米中桔霉素的快速检测方法研究[J].食品科技,2012(11):297-299.
[17]Zhou Y,Chen J,Dong L,et al.A study of fluorescence properties of citrinin inβ-cyclodextrin aqueous solution and different solvents[J].Journal of Luminescence,2012,132(6):1437-1445.
[18]宫慧梅,赵树欣,邹海晏.用双向薄层层析检测红曲中的桔霉素[J].酿酒科技,2002(2):83-84.
[19]胡晓清,陈福生,邢淑婕,等.红曲中桔霉素的薄层层析分析[J].食品科学,2003,24(5):126-129.
[20]Abramson D,Usleber E,Mrtlbauer E.An indirect enzyme immunoassay for the mycotoxin citrinin[J].Applied&Environmental Microbiology,1995,61(5):2007-2009.
[21]陈福生,邢淑婕.红曲产品中桔霉素含量的ELISA测定[J].食品科学,2004,25(8):169-172.
[22]汪媛媛,李泳宁,郭养浩.双交联法制备桔霉素-蛋白质偶联抗原及抗体[J].生物化学与生物物理进展,2010(3):337-341.
[23]许赣荣,卢晨,穆晓青,等.部分红曲菌菌株产桔霉素的研究[J].无锡轻工大学学报,2000,19(1):58-61.
[24]文镜,贾继春.高效液相色谱法测定红曲醋中桔霉素含量[C].全国第三届功能性生物制品生产与应用技术交流会,2008.
[25]陈蕴,许赣荣,虞慧玲.红曲桔霉素高效液相色谱测定条件的优化[J].食品与发酵工业,2004,30(1):118-123.
[26]李志强,袁永俊,张晓龙.高效液相色谱法测定桔霉素[J].中国调味品,2011,36(4):72-75.
[27]Bing-hui L,Ting-shuan W,Mao-chang S,et al.Evaluation of citrinin occurrence and cytotoxicity in Monascus fermentation product[J].Journal of Agricultural&Food Chemistry,2005,53(1):170-175.
[28]陈军,李章万,祁伟,等.高效毛细管电泳分析法检测中药红曲的桔霉素含量[J].中国中药杂志,2007,32(14):1412-1415.
[29]甘纯玑,蔡春燕.薄层色谱法分离红曲色素[J].精细化工,1989(5):51-53.
[30]王伟民,张毓华.红曲色素的测定[J].上海调味品,1986(1):20-21.
[31]傅亮,周卫兵,高孔荣.红曲色素高产菌株发酵特性的研究[J].食品科学,1996(3):6-9.
[32]毛瑞丰.红曲米中红曲菌的分纯及红曲色素测定[J].轻工科技,2003(1):29-30.
[33]孙艳君,胡中泽,高冰.红曲菌的分离鉴定及红曲色素的测定[J].中国酿造,2011(1):52-54.
[34]钟立人,蒋家新,毕丽君.红曲色素组分分离的研究[J].食品与机械,2000(1):20-21.
[35]张慧娟,陶冠军,陈蕴,等.红曲色素的制备及HPLC和LC/MS检测方法[J].食品研究与开发,2006,27(4):112-115.
[36]崔莉,胡晓丹,张德权.HPLC法同时测定红曲色素中的红曲素和安卡红曲黄素[J].食品科学,2009,30(8):163-166.
[37]胡丽芳,罗林广,李瑞丽,等.肉制品中5种红曲色素的高效液相色谱测定[J].江西农业学报,2012,24(10):104-106.
[38]储怡士,卢普滨,周瑶敏,等.一种检测肉制品中红曲色素含量的方法[P].中国专利:102565227A,2012.
[39]Campoy S,Rumbero A,Martín J F,et al.Characterization of an hyperpigmenting mutant of Monascus purpureus IB1:identification of two novel pigment chemical structures[J].Applied Microbiology&Biotechnology,2006,70(4):488-496.
[40]Teng S S,Feldheim W.Analysis of anka pigments by liquid chromatography with diode array detection and tandem mass spectrometry[J].Chromatographia,1998,47(9):529-536.
[41]马书民,张勋,芦春梅,等.液相色谱串联质谱法测定食品中红曲色素[J].食品与发酵工业,2013,39(6):191-194.
[42]张晓伟,王加华,王昌禄,等.基于近红外光谱技术检测红曲米中的红曲色素[J].现代食品科技,2014(5):273-279.
[43]陈松生,毛宁.红曲菌的麦角甾醇研究[J].食品与发酵工业,1995(6):18-23.
[44]陈松生,毛宁,陈哲超,等.红曲菌产生的生理活性物质的研究Ⅱ.红曲菌菌株产生麦角甾醇的研究[J].福建师范大学学报:自然科学版,1995(1):79-84.
[45]朱效刚,陶冠军,陈蕴,等.高效液相色谱法测定功能性红曲中的麦角甾醇[J].食品与发酵工业,2004,30(9):104-107.
[46]谷学新,赵红帅,邹洪,等.高效液相色谱法测定功能性红曲米粉中的麦角甾醇[J].首都师范大学学报:自然科学版,2006,27(1):78-79.
[47]林杰,黄宏南,林国斌,等.功能性红曲功效成分的含量测定[J].中国卫生检验杂志,2007(6):972-973.
[48]赵颖.红曲菌产GABA相关基因的初步研究[D].武汉:华中农业大学,2007.
[49]李利,陈莎,赵颖,等.快速测定红曲中γ-氨基丁酸含量的薄层色谱-比色法研究[J].长江大学学报自然科学版:农学卷,2012(12):23-26.
[50]秦江辉,周礼红,胡开成,等.高产γ-氨基丁酸的红曲菌菌株选育[J].江苏农业科学,2012,40(3):320-322.
[51]张庆庆,刘辉,汤斌,等.柱前衍生化毛细管电泳法测定红曲酒中γ-氨基丁酸含量[J].食品与发酵工业,2009(12):119-122.
[52]刘辉.红曲菌发酵提高γ-氨基丁酸含量的研究[D].芜湖:安徽工程大学,2010.
[53]马莉锋,周立平,孙佰申,等.HPLC-ELSD法定量分析红曲菌发酵样品中的γ-氨基丁酸[J].酿酒科技,2004(6):88-89.
[54]丁献荣,提高兰,蒋涛,等.高效液相色谱法测定红曲酒糟发酵物中γ-氨基丁酸的含量[J].时代经贸,2010(6):268-269.
[55]张圆林,王昌禄,陈勉华,等.高产γ-氨基丁酸的红曲菌菌株筛选及发酵条件优化[J].食品科学技术学报,2014,32(5):35-40.
[2]吴芳彤.高产洛伐他汀红曲菌的分离鉴定及其红曲发酵条件的优化[D].福州:福建农林大学,2014.
[3]马美荣,王正祥.红曲有效生理活性物质的研究现状与进展[J].酿酒科技,1999(5):26-27.
[4]魏巍.红曲霉发酵合成洛伐他汀的研究[D].武汉:华中科技大学,2013.
[5]曹纪亮,孔维军,杨美华,等.真菌毒素快速检测方法研究进展[J].药物分析杂志,2013(1):159-164.
[6]杨丽,张水华,王启军.TLC薄层色谱法同步检测功能红曲中酸型和内酯型莫那呵啉K(Monacolin K)[J].食品研究与开发,2006,27(2):113-114.
[7]汤卫华,乔长晟,许春英.紫外分光光度法检测Monacolin K的含量[J].食品工业科技,2004,25(4):129-130.
[8]文镜,顾晓玲,常平等.双波长紫外分光光度法测定红曲中洛伐他汀的含量[J].中国食品添加剂,2000(4):11-17.
[9]张庆庆,危勤涛,汤斌,等.毛细管电泳法测定红曲胶囊中洛伐他汀含量[J].中国实验方剂学杂志,2008(11):4-6.
[10]Nigovic B,Sertic M,Mornar A.Simultaneous determination of lovastatin and citrinin in red yeast rice supplements by micellar electrokinetic capillary chromatography[J].Food Chem,2013,138(1):531-538.
[11]Friedrich J,uek M,Bencˇina M,et al.High-performance liquid chromatographic analysis of mevinolin as mevinolinic acid in fermentation broths[J].Journal of Chromatography A,1995,704(2):363-367.
[12]Su Y C,Wang J J,Lin T T,et al.Production of the secondary metabolites gamma-aminobutyric acid and monacolin K by Monascus[J].Journal of Industrial Microbiology&Biotechnology,2003(30):41-46.
[13]Lee C L,Wang J J,Pan T M.Synchronous analysis method for detection of citrinin and the lactone and acid forms of monacolin K in red mold rice[J].Journal of Aoac International,2006,89(3):669-677.
[14]Ajdari Z,Ebrahimpour A,Abdul M M,et al.Assessment of monacolin in the fermented products using Monascus purpureus FTC5391[J].Biomed Research International,2011(1):426168-426168.
[15]Song F,El-demerdash A,Lee S J S H,et al.Fast screening of lovastatin in red yeast rice products by flow injection tandem mass spectrometry[J].Journal of Pharmaceutical&Biomedical Analysis,2011,57(2):76-81.
[16]陈奇,王伟平,张华山,等.红曲米中桔霉素的快速检测方法研究[J].食品科技,2012(11):297-299.
[17]Zhou Y,Chen J,Dong L,et al.A study of fluorescence properties of citrinin inβ-cyclodextrin aqueous solution and different solvents[J].Journal of Luminescence,2012,132(6):1437-1445.
[18]宫慧梅,赵树欣,邹海晏.用双向薄层层析检测红曲中的桔霉素[J].酿酒科技,2002(2):83-84.
[19]胡晓清,陈福生,邢淑婕,等.红曲中桔霉素的薄层层析分析[J].食品科学,2003,24(5):126-129.
[20]Abramson D,Usleber E,Mrtlbauer E.An indirect enzyme immunoassay for the mycotoxin citrinin[J].Applied&Environmental Microbiology,1995,61(5):2007-2009.
[21]陈福生,邢淑婕.红曲产品中桔霉素含量的ELISA测定[J].食品科学,2004,25(8):169-172.
[22]汪媛媛,李泳宁,郭养浩.双交联法制备桔霉素-蛋白质偶联抗原及抗体[J].生物化学与生物物理进展,2010(3):337-341.
[23]许赣荣,卢晨,穆晓青,等.部分红曲菌菌株产桔霉素的研究[J].无锡轻工大学学报,2000,19(1):58-61.
[24]文镜,贾继春.高效液相色谱法测定红曲醋中桔霉素含量[C].全国第三届功能性生物制品生产与应用技术交流会,2008.
[25]陈蕴,许赣荣,虞慧玲.红曲桔霉素高效液相色谱测定条件的优化[J].食品与发酵工业,2004,30(1):118-123.
[26]李志强,袁永俊,张晓龙.高效液相色谱法测定桔霉素[J].中国调味品,2011,36(4):72-75.
[27]Bing-hui L,Ting-shuan W,Mao-chang S,et al.Evaluation of citrinin occurrence and cytotoxicity in Monascus fermentation product[J].Journal of Agricultural&Food Chemistry,2005,53(1):170-175.
[28]陈军,李章万,祁伟,等.高效毛细管电泳分析法检测中药红曲的桔霉素含量[J].中国中药杂志,2007,32(14):1412-1415.
[29]甘纯玑,蔡春燕.薄层色谱法分离红曲色素[J].精细化工,1989(5):51-53.
[30]王伟民,张毓华.红曲色素的测定[J].上海调味品,1986(1):20-21.
[31]傅亮,周卫兵,高孔荣.红曲色素高产菌株发酵特性的研究[J].食品科学,1996(3):6-9.
[32]毛瑞丰.红曲米中红曲菌的分纯及红曲色素测定[J].轻工科技,2003(1):29-30.
[33]孙艳君,胡中泽,高冰.红曲菌的分离鉴定及红曲色素的测定[J].中国酿造,2011(1):52-54.
[34]钟立人,蒋家新,毕丽君.红曲色素组分分离的研究[J].食品与机械,2000(1):20-21.
[35]张慧娟,陶冠军,陈蕴,等.红曲色素的制备及HPLC和LC/MS检测方法[J].食品研究与开发,2006,27(4):112-115.
[36]崔莉,胡晓丹,张德权.HPLC法同时测定红曲色素中的红曲素和安卡红曲黄素[J].食品科学,2009,30(8):163-166.
[37]胡丽芳,罗林广,李瑞丽,等.肉制品中5种红曲色素的高效液相色谱测定[J].江西农业学报,2012,24(10):104-106.
[38]储怡士,卢普滨,周瑶敏,等.一种检测肉制品中红曲色素含量的方法[P].中国专利:102565227A,2012.
[39]Campoy S,Rumbero A,Martín J F,et al.Characterization of an hyperpigmenting mutant of Monascus purpureus IB1:identification of two novel pigment chemical structures[J].Applied Microbiology&Biotechnology,2006,70(4):488-496.
[40]Teng S S,Feldheim W.Analysis of anka pigments by liquid chromatography with diode array detection and tandem mass spectrometry[J].Chromatographia,1998,47(9):529-536.
[41]马书民,张勋,芦春梅,等.液相色谱串联质谱法测定食品中红曲色素[J].食品与发酵工业,2013,39(6):191-194.
[42]张晓伟,王加华,王昌禄,等.基于近红外光谱技术检测红曲米中的红曲色素[J].现代食品科技,2014(5):273-279.
[43]陈松生,毛宁.红曲菌的麦角甾醇研究[J].食品与发酵工业,1995(6):18-23.
[44]陈松生,毛宁,陈哲超,等.红曲菌产生的生理活性物质的研究Ⅱ.红曲菌菌株产生麦角甾醇的研究[J].福建师范大学学报:自然科学版,1995(1):79-84.
[45]朱效刚,陶冠军,陈蕴,等.高效液相色谱法测定功能性红曲中的麦角甾醇[J].食品与发酵工业,2004,30(9):104-107.
[46]谷学新,赵红帅,邹洪,等.高效液相色谱法测定功能性红曲米粉中的麦角甾醇[J].首都师范大学学报:自然科学版,2006,27(1):78-79.
[47]林杰,黄宏南,林国斌,等.功能性红曲功效成分的含量测定[J].中国卫生检验杂志,2007(6):972-973.
[48]赵颖.红曲菌产GABA相关基因的初步研究[D].武汉:华中农业大学,2007.
[49]李利,陈莎,赵颖,等.快速测定红曲中γ-氨基丁酸含量的薄层色谱-比色法研究[J].长江大学学报自然科学版:农学卷,2012(12):23-26.
[50]秦江辉,周礼红,胡开成,等.高产γ-氨基丁酸的红曲菌菌株选育[J].江苏农业科学,2012,40(3):320-322.
[51]张庆庆,刘辉,汤斌,等.柱前衍生化毛细管电泳法测定红曲酒中γ-氨基丁酸含量[J].食品与发酵工业,2009(12):119-122.
[52]刘辉.红曲菌发酵提高γ-氨基丁酸含量的研究[D].芜湖:安徽工程大学,2010.
[53]马莉锋,周立平,孙佰申,等.HPLC-ELSD法定量分析红曲菌发酵样品中的γ-氨基丁酸[J].酿酒科技,2004(6):88-89.
[54]丁献荣,提高兰,蒋涛,等.高效液相色谱法测定红曲酒糟发酵物中γ-氨基丁酸的含量[J].时代经贸,2010(6):268-269.
[55]张圆林,王昌禄,陈勉华,等.高产γ-氨基丁酸的红曲菌菌株筛选及发酵条件优化[J].食品科学技术学报,2014,32(5):35-40.