河南粳稻新品系的遗传多样性分析
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摘要
为探究河南粳稻遗传多样性,利用水稻农艺性状和SSR分子标记对22份粳稻新品系进行遗传多样性分析。结果表明,在13对引物中共检测出40个等位基因,每个位点平均3.1个等位基因,引物多态性指数为0.208,平均遗传相似系数为0.701。8个农艺性状主成分分析发现,生育期是反映品系间差异的最主要成分。以遗传相似性系数和生育期为指标聚类分析,在欧氏距离为9处将22份粳稻新品系分为4大类。豫南粳稻的多态性位点、多态位点百分率(PPB)、Nei's基因多样性指数(H值)、Shannon's多态性信息指数(I值)高于沿黄粳稻,生育期比沿黄粳稻短。
To explore the genetic diversity of Henan japonica rice, 22 new varieties of japonica rice were used to analyze genetic diversity with agronomic traits and SSR molecular markers. The results showed that 40 polymorphic bands were detected from the 13 SSR primers, with the average of 3.1 bands per pair of primers. The index of polymorphism of primers was 0.208, and the average genetic similarity coefficient was 0.701. In terms of the principal component analysis with the 8 agronomic traits, the growth period was the main principal component to reflect differences among varieties. For the cluster analysis with genetic similarity coefficient and growth period,22 new varieties of japonica rice were divided into 4 clusters at the Euclidean distance of 9. The polymorphic locus, the values of percentage of polymorphic bands(PPB), the Nei's gene diversity index(H) and Shannon's polymorphism information index(I) of japonica in southern Henan were higher than that in regions alongside Huanghe river of Henan, but the growth period of japonica in Yunan were shorter than that in Yanhuang.
To explore the genetic diversity of Henan japonica rice, 22 new varieties of japonica rice were used to analyze genetic diversity with agronomic traits and SSR molecular markers. The results showed that 40 polymorphic bands were detected from the 13 SSR primers, with the average of 3.1 bands per pair of primers. The index of polymorphism of primers was 0.208, and the average genetic similarity coefficient was 0.701. In terms of the principal component analysis with the 8 agronomic traits, the growth period was the main principal component to reflect differences among varieties. For the cluster analysis with genetic similarity coefficient and growth period,22 new varieties of japonica rice were divided into 4 clusters at the Euclidean distance of 9. The polymorphic locus, the values of percentage of polymorphic bands(PPB), the Nei's gene diversity index(H) and Shannon's polymorphism information index(I) of japonica in southern Henan were higher than that in regions alongside Huanghe river of Henan, but the growth period of japonica in Yunan were shorter than that in Yanhuang.
引文
Chen X.L.,Ma L.F.,Wang P.,Wang X.L.,Li Y.T.,Li P.,Ma X.Y.,and Li P.F.,2013,Genetic diversity of 60 japonica rice germplasms in Ningxia province,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),14(2):226-231(陈小龙,马利奋,王鹏,王学龙,李亚婷,李鹏,马晓燕,李培富,2013,宁夏60份粳稻种质资源遗传多样性分析,植物遗传资源学报,14(2):226-231)
Guo G.Z.,Zhang K.L.,Yang X.Y.,and Qiu Z.G.,2011,On rice variety improvement and parental matching in Jilin province,Shandong Nongye Kexue(Shandong Agricultural Science),(11):20-22,43(郭桂珍,张奎林,杨秀云,邱志刚,2011,论吉林省水稻品种改良及亲本选配,山东农业科学,(11):20-22,43)
Han Y.C.,Li J.Z.,Guo M.J.,and Zhao Q.Z.,2012,Establishment of SSR fingerprint map and analysis of genetic similarity among the japonica rice(Oryza sativa L.)of Henan,Nongye Shengwu Jishu Xuebao(Journal of Agricultural Biotechnology),20(12):1378-1389(韩迎春,李俊周,郭敏杰,赵全志,2012,河南粳稻品种SSR指纹图谱的构建及遗传差异分析,农业生物技术学报,20(12):1378-1389)
Jin W.D.,and Hong D.L.,2006,Genetic diversity in japonica rice landraces(Oryza sativa L.)from the Taihu Lake region,Shengwu Duoyangxing(Biodiversity Science),14(6):479-487(金伟栋,洪德林,2006,太湖流域粳稻地方品种遗传多样性研究,生物多样性,14(6):479-487)
Li G.G.,Zhang H.,Zheng Y.S.,and Li R.H.,2018,The genetic diversity analysis of Chinese flowering cabbage resources based on SSR marker,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),37(3):1257-1264(李光光,张华,郑岩松,李荣华,2018,利用SSR标记研究菜心资源的遗传多样性分析,基因组学与应用生物学,37(3):1257-1264)
Li X.X.,Wang Q.,Luo S.X.,He Y.X.,Zhu L.H.,Zhou Y.L.,and Li Z.K.,2012,Analyzing avirulence genes of Magnaporthe oryzae from Heilongjiang province and screening rice germplasm with resistance to Blast Fungus,Zuowu Xuebao(The Crop Journal),38(12):2192-2197(李祥晓,王倩,罗生香,何云霞,朱苓华,周永力,黎志康,2012,黑龙江省稻瘟病菌无毒基因分析及抗病种质资源筛选,作物学报,38(12):2192-2197)
Luo R.,Wu W.L.,Zhang Y.,and Li Y.H.,2010,Application of SSR molecular markers in crop genetics and breeding,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),29(1):137-143(罗冉,吴委林,张旸,李玉花,2010,SSR分子标记在作物遗传育种中的应用,基因组学与应用生物学,29(1):137-143)
Ma Z.B.,Wang C.H.,Wang H.,and Fu L.,2014,Analysis on genetic diversity of rice varieties from different countries,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),15(3):540-545(马作斌,王昌华,王辉,付亮,2014,不同国家水稻品种的遗传多样性分析,植物遗传资源学报,15(3):540-545)
Murray M.G.,and Thompson W.F.,1980,Rapid isolation of high molecular weight plant DNA,Nucleic Acids Res.,8(19):4321-4325
Tian Z.X.,Qian Q.,Liu Q.Q.,Yan M.X.,Liu X.F.,Yan C.J.,Liu G.F.,Gao Z.Y.,Tang S.Z.,Zeng D.L.,Wang Y.H.,Yu J.M.,Gu M.H.,and Li J.Y.,2009,Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities,Proc.Natl.Acad.Sci.USA,106(51):21760-21765
Wang Q.,Zhou Y.L.,Wang S.,and Li Z.K.,2012,Research advances on the rice blast in the northeast China,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),13(1):143-147(王倩,周永力,王疏,黎志康,2012,我国东北稻区稻瘟病的研究进展,植物遗传资源学报,13(1):143-147)
Xu D.Y.,Zhong H.,Zhou F.,Lu B.G.,Fan J.W.,Fang Z.W.,Qin D.R.,and Wan J.M.,2010,Genetic diversity of mid-ripen Japonica varietiesⅡ.Comparison of genetic diversity of Japonica varieties bred recently in Huang-huai region by SSR markers,Jiangsu Nongye Xuebao(Jiangsu Journal of Agricultural Science),26(1):15-21(徐大勇,钟环,周峰,卢百关,樊继伟,方兆伟,秦德荣,万建民,2010,中粳水稻品种资源的遗传多样性Ⅱ.黄淮稻区近期育成品种的SSR多样性比较,江苏农业学报,26(1):15-21)
You S.M.,Cao Y.J.,Zheng J.K.,Jiang K.F.,Zhang T.,Yang L.,Yang Q.H.,Wan X.Q.,Luo J.,Li Z.X.,and Gao L.,2015,Principal component and cluster analysis for agronomic traits of 73 rice restorers lines in Asia,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(2):250-256(游书梅,曹应江,郑家奎,蒋开锋,张涛,杨莉,杨乾华,万先齐,罗婧,李昭祥,高磊,2015,73份亚洲水稻恢复系农艺性状的主成分与聚类分析,植物遗传资源学报,16(2):250-256)
Zhang K.,Wei H.F.,Zhuo D.L.,Zhang X.J.,Zhang F.,Zhou Y.L.,and Li Z.K.,2016,Genetic diversity snalysis of registered rice(Oryza sativa L.)varieties in Heilongjiang province based on SSR markers,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),17(3):447-454(张科,魏海锋,卓大龙,张晓敬,张帆,周永力,黎志康,2016,黑龙江省近年审定水稻品种基于SSR标记的遗传多样性分析,植物遗传资源学报,17(3):447-454)
Zhang L.N.,Cao G.L.,and Han L.Z.,2012,Analysis of genetic diversity of japonica rice landrace in China with microsatellite marker,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),45(3):405-413(张立娜,曹桂兰,韩龙植,2012,利用SSR标记揭示中国粳稻地方品种遗传多样性,中国农业科学,45(3):405-413)
Zhao G.Z.,Shi R.,Liu J.X.,Zou Q.,Liu W.H.,Zhu Z.H.,Kou S.Y.,Chen Y.M.,Su Z.X.,and Yuan P.R.,2015,Analysis of agronomic characters for inbred japonica rice in Yunnan Plateau,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),28(5):1857-1862(赵国珍,世荣,刘吉新,邹茜,刘慰华,朱振华,寇姝燕,陈于敏,苏振喜,袁平荣,2015,云南高原常规粳稻主栽品种农艺性状分析,西南农业学报,28(5):1857-1862)
Zhou Z.L.,Wang B.X.,Fan W.J.,LüB.G.,Zhao Z.G.,Jiang L.,Qin D.R.,Wan J.M.,and Xu D.Y.,2012,Indica-japonica difference degree and genetic diversity of japonica cultivars belonging to different ecotypes from the Yangtze-Huaihe region,Zhongguo Shuidao Kexue(Chinese Journal Rice Science),26(4):431-437(周振玲,王宝祥,樊继伟,卢百关,赵志刚,江玲,秦德荣,万建民,徐大勇,2012,江淮稻区不同生态型粳稻品种的籼粳分化度和遗传多样性,中国水稻科学,26(4):431-437)
Zhu Y.Y.,Chen H.R.,Fan J.H.,Wang Y.Y.,Li Y.,Chen J.B.,Fan J.X.,Yang S.S.,Hu L.P.,Hei L.,Tom W.M.,Paul S.T.,Zong H.W.,and Christopher C.M.,2000,Genetic diversity and disease control in rice,Nature,406:718-722
Zhuang J.Y.,Qian H.R.,and Zheng K.L.,1998,Screening of highly-polymorphic RFLP probes in Oryza sativa L.,Journal Genetics and Breeding,52(1):39-48
Guo G.Z.,Zhang K.L.,Yang X.Y.,and Qiu Z.G.,2011,On rice variety improvement and parental matching in Jilin province,Shandong Nongye Kexue(Shandong Agricultural Science),(11):20-22,43(郭桂珍,张奎林,杨秀云,邱志刚,2011,论吉林省水稻品种改良及亲本选配,山东农业科学,(11):20-22,43)
Han Y.C.,Li J.Z.,Guo M.J.,and Zhao Q.Z.,2012,Establishment of SSR fingerprint map and analysis of genetic similarity among the japonica rice(Oryza sativa L.)of Henan,Nongye Shengwu Jishu Xuebao(Journal of Agricultural Biotechnology),20(12):1378-1389(韩迎春,李俊周,郭敏杰,赵全志,2012,河南粳稻品种SSR指纹图谱的构建及遗传差异分析,农业生物技术学报,20(12):1378-1389)
Jin W.D.,and Hong D.L.,2006,Genetic diversity in japonica rice landraces(Oryza sativa L.)from the Taihu Lake region,Shengwu Duoyangxing(Biodiversity Science),14(6):479-487(金伟栋,洪德林,2006,太湖流域粳稻地方品种遗传多样性研究,生物多样性,14(6):479-487)
Li G.G.,Zhang H.,Zheng Y.S.,and Li R.H.,2018,The genetic diversity analysis of Chinese flowering cabbage resources based on SSR marker,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),37(3):1257-1264(李光光,张华,郑岩松,李荣华,2018,利用SSR标记研究菜心资源的遗传多样性分析,基因组学与应用生物学,37(3):1257-1264)
Li X.X.,Wang Q.,Luo S.X.,He Y.X.,Zhu L.H.,Zhou Y.L.,and Li Z.K.,2012,Analyzing avirulence genes of Magnaporthe oryzae from Heilongjiang province and screening rice germplasm with resistance to Blast Fungus,Zuowu Xuebao(The Crop Journal),38(12):2192-2197(李祥晓,王倩,罗生香,何云霞,朱苓华,周永力,黎志康,2012,黑龙江省稻瘟病菌无毒基因分析及抗病种质资源筛选,作物学报,38(12):2192-2197)
Luo R.,Wu W.L.,Zhang Y.,and Li Y.H.,2010,Application of SSR molecular markers in crop genetics and breeding,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),29(1):137-143(罗冉,吴委林,张旸,李玉花,2010,SSR分子标记在作物遗传育种中的应用,基因组学与应用生物学,29(1):137-143)
Ma Z.B.,Wang C.H.,Wang H.,and Fu L.,2014,Analysis on genetic diversity of rice varieties from different countries,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),15(3):540-545(马作斌,王昌华,王辉,付亮,2014,不同国家水稻品种的遗传多样性分析,植物遗传资源学报,15(3):540-545)
Murray M.G.,and Thompson W.F.,1980,Rapid isolation of high molecular weight plant DNA,Nucleic Acids Res.,8(19):4321-4325
Tian Z.X.,Qian Q.,Liu Q.Q.,Yan M.X.,Liu X.F.,Yan C.J.,Liu G.F.,Gao Z.Y.,Tang S.Z.,Zeng D.L.,Wang Y.H.,Yu J.M.,Gu M.H.,and Li J.Y.,2009,Allelic diversities in rice starch biosynthesis lead to a diverse array of rice eating and cooking qualities,Proc.Natl.Acad.Sci.USA,106(51):21760-21765
Wang Q.,Zhou Y.L.,Wang S.,and Li Z.K.,2012,Research advances on the rice blast in the northeast China,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),13(1):143-147(王倩,周永力,王疏,黎志康,2012,我国东北稻区稻瘟病的研究进展,植物遗传资源学报,13(1):143-147)
Xu D.Y.,Zhong H.,Zhou F.,Lu B.G.,Fan J.W.,Fang Z.W.,Qin D.R.,and Wan J.M.,2010,Genetic diversity of mid-ripen Japonica varietiesⅡ.Comparison of genetic diversity of Japonica varieties bred recently in Huang-huai region by SSR markers,Jiangsu Nongye Xuebao(Jiangsu Journal of Agricultural Science),26(1):15-21(徐大勇,钟环,周峰,卢百关,樊继伟,方兆伟,秦德荣,万建民,2010,中粳水稻品种资源的遗传多样性Ⅱ.黄淮稻区近期育成品种的SSR多样性比较,江苏农业学报,26(1):15-21)
You S.M.,Cao Y.J.,Zheng J.K.,Jiang K.F.,Zhang T.,Yang L.,Yang Q.H.,Wan X.Q.,Luo J.,Li Z.X.,and Gao L.,2015,Principal component and cluster analysis for agronomic traits of 73 rice restorers lines in Asia,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(2):250-256(游书梅,曹应江,郑家奎,蒋开锋,张涛,杨莉,杨乾华,万先齐,罗婧,李昭祥,高磊,2015,73份亚洲水稻恢复系农艺性状的主成分与聚类分析,植物遗传资源学报,16(2):250-256)
Zhang K.,Wei H.F.,Zhuo D.L.,Zhang X.J.,Zhang F.,Zhou Y.L.,and Li Z.K.,2016,Genetic diversity snalysis of registered rice(Oryza sativa L.)varieties in Heilongjiang province based on SSR markers,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),17(3):447-454(张科,魏海锋,卓大龙,张晓敬,张帆,周永力,黎志康,2016,黑龙江省近年审定水稻品种基于SSR标记的遗传多样性分析,植物遗传资源学报,17(3):447-454)
Zhang L.N.,Cao G.L.,and Han L.Z.,2012,Analysis of genetic diversity of japonica rice landrace in China with microsatellite marker,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),45(3):405-413(张立娜,曹桂兰,韩龙植,2012,利用SSR标记揭示中国粳稻地方品种遗传多样性,中国农业科学,45(3):405-413)
Zhao G.Z.,Shi R.,Liu J.X.,Zou Q.,Liu W.H.,Zhu Z.H.,Kou S.Y.,Chen Y.M.,Su Z.X.,and Yuan P.R.,2015,Analysis of agronomic characters for inbred japonica rice in Yunnan Plateau,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),28(5):1857-1862(赵国珍,世荣,刘吉新,邹茜,刘慰华,朱振华,寇姝燕,陈于敏,苏振喜,袁平荣,2015,云南高原常规粳稻主栽品种农艺性状分析,西南农业学报,28(5):1857-1862)
Zhou Z.L.,Wang B.X.,Fan W.J.,LüB.G.,Zhao Z.G.,Jiang L.,Qin D.R.,Wan J.M.,and Xu D.Y.,2012,Indica-japonica difference degree and genetic diversity of japonica cultivars belonging to different ecotypes from the Yangtze-Huaihe region,Zhongguo Shuidao Kexue(Chinese Journal Rice Science),26(4):431-437(周振玲,王宝祥,樊继伟,卢百关,赵志刚,江玲,秦德荣,万建民,徐大勇,2012,江淮稻区不同生态型粳稻品种的籼粳分化度和遗传多样性,中国水稻科学,26(4):431-437)
Zhu Y.Y.,Chen H.R.,Fan J.H.,Wang Y.Y.,Li Y.,Chen J.B.,Fan J.X.,Yang S.S.,Hu L.P.,Hei L.,Tom W.M.,Paul S.T.,Zong H.W.,and Christopher C.M.,2000,Genetic diversity and disease control in rice,Nature,406:718-722
Zhuang J.Y.,Qian H.R.,and Zheng K.L.,1998,Screening of highly-polymorphic RFLP probes in Oryza sativa L.,Journal Genetics and Breeding,52(1):39-48
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