设计左旋糖酐——果糖的合成实验探索与综合改进
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摘要
开展物理化学综合性设计型实验教学改革,研究蔗糖转化实验,探索设计左旋糖酐(果糖)的合成实验研究.利用旋光仪测旋光度的方法监测溶液中蔗糖的浓度变化情况,从而测得其转化率及动力学规律.根据果糖和葡萄糖的钙离子络合物的性质差异实现纯化和有效分离、洗涤、干燥,获得要求的产品,并引入薄层色谱实现快速检测.建立起一项完整的实验教学项目,有效促进探究型实验教学发展,同时完善综合性设计型实验,进一步有效落实教学改革.
To carry out the comprehensive and disignating reform of the education of the physical chemistry experiments,and to explore the experimental conditions of the transformation reaction of sucrose into glucose and fructose,and to explore changes and variation of the synthesis experiment of the design of levulose-fructose,optical rotatory activity was used to monitor the change of sucrose and glucose and fructose concentration in the solution,then the conversion rate and kinetic law were measured out.According to the difference of the properties of the calcium ion complex of fructose and glucose,the purified and effective separation,washing and drying were achieved,and the required products were obtained,and the rapid detection was realized by the thin layer chromatography of silica gel.A complete experimental teaching project should be set up to promote the development of exploratively experimental teaching,so as to improve the comprehensive design experiment and carry out the teaching reform effectively.
To carry out the comprehensive and disignating reform of the education of the physical chemistry experiments,and to explore the experimental conditions of the transformation reaction of sucrose into glucose and fructose,and to explore changes and variation of the synthesis experiment of the design of levulose-fructose,optical rotatory activity was used to monitor the change of sucrose and glucose and fructose concentration in the solution,then the conversion rate and kinetic law were measured out.According to the difference of the properties of the calcium ion complex of fructose and glucose,the purified and effective separation,washing and drying were achieved,and the required products were obtained,and the rapid detection was realized by the thin layer chromatography of silica gel.A complete experimental teaching project should be set up to promote the development of exploratively experimental teaching,so as to improve the comprehensive design experiment and carry out the teaching reform effectively.
引文
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[14] 潘娅.探究型实验教学在药剂学中的实践与成效[J].广州化工,2015,43(14):213-214.
[15] 赵华绒,秦敏锐,蔡黄菊,等.如何发挥实验技术人员在探究型实验教学中的作用[J].实验室研究与探索,2017,36(2):153-156.
[2] 李田,徐金荣,杨玲,等.实验技术人员如何在实验教学中发挥作用[J].实验技术与管理.2015,32(6):143-145.
[3] 北京大学化学系物理化学教研室.物理化学实验[M].4版.北京:北京大学出版社,2018,80-84.
[4] 复旦大学等,庄继华等修订.物理化学实验[M].3版.北京:高等教育出版社,2005,94-98.
[5] 殷悦,洪琴,孙凯铖,等.蔗糖水解反应速率常数测定实验数据处理方法的改进[J].当代化工,2018,47(2):256-259,263.
[6] 李洪仁,胡天慧.物理化学实验数据的计算机处理——一级反应蔗糖转化数据处理[J].沈阳大学学报:自然科学版,2012,24(3):35-37.
[7] 许丽梅,徐旭耀,马琳,蔗糖水解反应速率常数测定实验的设计及改进[J].实验室科学,2016,19(1):23-25.
[8] 祁元明,生物化学实验原理与技术(可溶性糖的硅胶板薄层层析)[M].北京:化学工业出版社,2011.
[9] 杨凤丽,梁国斌,顾宇阳.H2SO4改性凹凸棒土在葡萄糖脱水制备5-羟甲基糠醛过程中的催化性能研究[J].轻工学报,2019,34(1):64-70.
[10] 石宁,刘琪英,王铁军,等.葡萄糖催化脱水制取 5-羟甲基糠醛研究进展[J].化工进展,2012,31(4):792-800.
[11] 周智华,李国斌,唐安平,等.化工与材料类专业创新型实验教学体系的构建[J].当代教育理论与实践,2016,8(8):59-61.
[12] 陆靖,徐华龙.建设有利于化学创新人才培养的教学体制[J].大学化学,2003,18(4):11-12.
[13] 刘李,缪明星,丁启龙,等.药物代谢动力学综合性设计型实验教学改革与探讨[J].中南药学,2014 ,12(4):382-384.
[14] 潘娅.探究型实验教学在药剂学中的实践与成效[J].广州化工,2015,43(14):213-214.
[15] 赵华绒,秦敏锐,蔡黄菊,等.如何发挥实验技术人员在探究型实验教学中的作用[J].实验室研究与探索,2017,36(2):153-156.