穿龙薯蓣皂苷调节再生障碍性贫血免疫共刺激分子的研究
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摘要
目的:通过研究T细胞表达的共同刺激分子(CM)CD28、CD152、CD80、CD86在再生障碍性贫血(再障)小鼠的表达情况,探讨穿龙薯蓣皂苷治疗再障的作用机制。方法:用免疫介导法建立再障小鼠模型,随机分成模型组,高、中、低剂量组,雷公藤多苷组,环孢素组(Cs A组),并设正常组作为对照。造模第8天开始给药,正常组及模型组予等量0.9%氯化钠溶液。连续给药7d,眶丛静脉取血,对比外周血象,流式细胞术检测CD28、CD152、CD80、CD86的表达。结果:与正常组比较,模型组白细胞(WBC)、血红蛋白(HGB)及血小板(PLT)计数均显著减少(P<0.01),CD28、CD80、CD86的表达显著升高(P<0.05),CD152显著降低(P<0.05);与模型组比较,穿龙薯蓣皂苷各剂量组WBC、HGB及PLT计数均显著升高(P<0.05);中、高剂量组CD28、CD80、CD86的表达均显著减低,CD152的表达显著升高(P<0.05)。结论:穿龙薯蓣皂苷可能通过调节CM的表达从而发挥对再障的免疫调节作用。
Objective: To study the expression of co-stimulatory molecule(CM) CD28, CD152, CD80, CD86 expressed in T-lymphocytes in mice aplastic anemia(AA) model, and to explore the mechanism of dioscorea saponin to treat the disease. Methods: Evaluating the models of AA mice were established by immune-mediated method. Randomly dividing mice into 7 groups: model group, high, middle groups, and low dose group, Tripterygium Wilfordii Polyglycoside group, Cs A group and normal group. From the eighth day of the experiment, the mice in the administration groups and normal group were fed with medicines and normal saline from the eighth day of the experiment. After continuous administration of 7d, blood samples from retro-orbital plexus were collected and detected, CD28, CD152, CD80 and CD86 were tested with flow cytometry. Results: Compared with normal group, WBC, HGB, PLT counts and CD152 in model group were significantly decreased(P<0.01, P<0.05). CD28, CD80, CD86 in middle, high dose groups were significantly increased(P<0.05). Compared with the model group, WBC, HGB, PLT counts in high, middle, low dose groups were significantly increased(P<0.05), CD28, CD80, CD86 in middle, high dose groups were significanty decreased(P<0.05), CD152 in middle, high dose groups were significantly increased(P<0.05). Conclusion: It is possible that the expression of the CM maybe the immunology mechanism of the Dioscorea Saponin to treat AA.
Objective: To study the expression of co-stimulatory molecule(CM) CD28, CD152, CD80, CD86 expressed in T-lymphocytes in mice aplastic anemia(AA) model, and to explore the mechanism of dioscorea saponin to treat the disease. Methods: Evaluating the models of AA mice were established by immune-mediated method. Randomly dividing mice into 7 groups: model group, high, middle groups, and low dose group, Tripterygium Wilfordii Polyglycoside group, Cs A group and normal group. From the eighth day of the experiment, the mice in the administration groups and normal group were fed with medicines and normal saline from the eighth day of the experiment. After continuous administration of 7d, blood samples from retro-orbital plexus were collected and detected, CD28, CD152, CD80 and CD86 were tested with flow cytometry. Results: Compared with normal group, WBC, HGB, PLT counts and CD152 in model group were significantly decreased(P<0.01, P<0.05). CD28, CD80, CD86 in middle, high dose groups were significantly increased(P<0.05). Compared with the model group, WBC, HGB, PLT counts in high, middle, low dose groups were significantly increased(P<0.05), CD28, CD80, CD86 in middle, high dose groups were significanty decreased(P<0.05), CD152 in middle, high dose groups were significantly increased(P<0.05). Conclusion: It is possible that the expression of the CM maybe the immunology mechanism of the Dioscorea Saponin to treat AA.
引文
[1]麻柔.免疫介导的造血抑制与骨髓衰竭.中国中西医结合杂志,2010,30(3):334-336
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[7]张新雪,张丰丰,赵宗江,等.补肾益髓生血法对60Co-γ联合环磷酰胺诱导再生障碍性贫血大鼠骨髓造血及免疫功能的影响.中华中医药杂志,2014,29(8):2583-2586,2714
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[9]Krummel Matthew F.Allison James P.CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation.J Exp Med,1995,182(2):459
[10]张囡,康廷国,尹海波.中药穿龙薯蓣化学成分与药理作用的研究进展.现代中药研究与实践,2010,24(6):87-90
[11]王宝庆,李学彬,田志发,等.穿山龙现代研究概况.中国林副特产,2010,108(5):96-97
[2]黄国清,刘秋爽,程永红,等.调节性T细胞及其分泌的细胞因子在再生障碍性贫血中的意义.中国免疫学杂志,2012,28(7):645-647
[3]盛芳园,何忠梅,陈凯,等.薯蓣皂苷元的提取分离、检测方法及药理作用研究进展.时珍国医国药,2013,24(4):914-917
[4]姚军,李树浓.淋巴细胞与再障关系的实验研究.中华血液学杂志,1991,12(5):229-23l
[5]刘振芳,孙汉英,刘文励,等.再障小鼠骨髓淋巴细胞Th1/Th2细胞亚群漂移与转录因子T-bet和GATA-3的调控作用.广西医科大学学报,2010,27(5):687-689
[6]张春青,徐从高,张锑,等.再生障碍性贫血患者共刺激信号系统的变化及意义.山东大学学报(医学版),2004,42(5):564-566
[7]张新雪,张丰丰,赵宗江,等.补肾益髓生血法对60Co-γ联合环磷酰胺诱导再生障碍性贫血大鼠骨髓造血及免疫功能的影响.中华中医药杂志,2014,29(8):2583-2586,2714
[8]肖仁普,周晓扬.T细胞共刺激信号系统研究进展.重庆文理学院学报(自然科学版),2007,26(6):83-88
[9]Krummel Matthew F.Allison James P.CD28 and CTLA-4 have opposing effects on the response of T cells to stimulation.J Exp Med,1995,182(2):459
[10]张囡,康廷国,尹海波.中药穿龙薯蓣化学成分与药理作用的研究进展.现代中药研究与实践,2010,24(6):87-90
[11]王宝庆,李学彬,田志发,等.穿山龙现代研究概况.中国林副特产,2010,108(5):96-97