多蕊商陆脂溶性成分的GC-MS分析
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摘要
目的分析多蕊商陆脂溶性化学成分。方法多蕊商陆全草的甲醇提取物加水后,用乙酸乙酯萃取,乙酸乙酯部分进行硅胶柱层析,石油醚洗脱部分得其脂溶性成分,通过气相色谱-质谱(GC-MS)联用技术,对其中的化学成分进行分析鉴定。结果通过计算机检索,鉴定了其中的45个化合物,占总量的97.73%,其中含量较大的组分依次是亚麻油酸、4-羟基-4-甲基-2-戊酮、1,3,5-三甲苯、苯乙烯、反式-9-十八碳烯酸、丙烯酸异辛酯等。结论多蕊商陆脂溶性化学成分主要为脂肪酸、烯烃类及芳香类化合物,其中的多种成分具有药理活性,为多蕊商陆的开发利用提供了依据。
Objective To analyze the liposoluble constituents of Phytolacca polyandra Batalin. Methods The plant material was extracted with Me OH. The extract was concentrated,added with water and then partitioned with Et OAc. The Et OAc part was chromatographed directly on a silica gel column and eluted with petroleum ether.The petroleum ether elution was concentrated and then analyzed by gas chromatography-mass spectrometry. Results45 components were identified by computer retrieval, accounting for 97.73% of the total. The major components were linolic acid, 4-hydroxy-4-methyl-2-pentanone, 1, 3, 5-trimethylbenzene, phenylethylene,trans-9-octadecenoic acid, 2-ethylhexyl acrylate. Conclusion The major compounds from the liposoluble fraction of Phytolacca polyandra Batalin were fatty acid, olefin and aromatic compounds, and many of them have pharmacological activities, which provided a basis for the development and utilization of P. polyandra Batalin.
Objective To analyze the liposoluble constituents of Phytolacca polyandra Batalin. Methods The plant material was extracted with Me OH. The extract was concentrated,added with water and then partitioned with Et OAc. The Et OAc part was chromatographed directly on a silica gel column and eluted with petroleum ether.The petroleum ether elution was concentrated and then analyzed by gas chromatography-mass spectrometry. Results45 components were identified by computer retrieval, accounting for 97.73% of the total. The major components were linolic acid, 4-hydroxy-4-methyl-2-pentanone, 1, 3, 5-trimethylbenzene, phenylethylene,trans-9-octadecenoic acid, 2-ethylhexyl acrylate. Conclusion The major compounds from the liposoluble fraction of Phytolacca polyandra Batalin were fatty acid, olefin and aromatic compounds, and many of them have pharmacological activities, which provided a basis for the development and utilization of P. polyandra Batalin.
引文
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[12]张卫江,曲红梅,孙兴华,等. 1,3,5-三甲苯联产1,2,4,5-四甲苯生产工艺的研究[J].天津大学学报,2002, 35(1):108-112.
[13]陈莉,张乐涛,蔡国星,等.丙烯酸异辛酯与1-辛烯的共聚研究[J].精细石油化工,2014, 31(3):80-83.
[2]刘媛琪,毛龙毅,闫荣玲,等.垂序商陆叶片总皂苷的提取及杀虫活性研究[J].植物资源与环境学报,2018, 27(2):33-38.
[3]周洁尘,周国英,刘君昂,等.垂序商陆及胜红蓟提取物对降香黄檀炭疽病菌的抑菌机理研究[J].植物保护,2017, 43(4):46-50.
[4]周倩,姚广涛,金若敏,等.中药商陆利尿作用的实验研究[J].山西医科大学学报,2014, 45(8):725-728.
[5]王鹏程,王秋红,赵珊,等.商陆化学成分及药理作用和临床应用研究进展[J].中草药,2014, 45(18):2722-2731.
[6]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,1996:17.
[7] YI Y H, ZHANG J M, ZHANG H C, et al. New Saponins from the Roots of Phytolacca polyandra[J]. Journal of Natural Products, 1995, 58(12):1880-1882.
[8]熊江,周俊,戴好富,等.多蕊商陆的化学成分[J].云南植物研究,2002, 24(3):401-405.
[9]王鹏程,赵珊,王秋红,等.商陆脂溶性成分的GC-MS分析及其利尿、致泻作用研究[J].辽宁中医药大学学报,2016, 18(2):15-17.
[10]李一飞,姚广涛.商陆药理作用及毒性研究进展[J].中国实验方剂学杂志,2011, 17(13):248-251.
[11]徐静,陆建平.气相色谱法测定茶叶中的脂肪酸组成[J].化工技术与开发,2009, 38(4):38-41.
[12]张卫江,曲红梅,孙兴华,等. 1,3,5-三甲苯联产1,2,4,5-四甲苯生产工艺的研究[J].天津大学学报,2002, 35(1):108-112.
[13]陈莉,张乐涛,蔡国星,等.丙烯酸异辛酯与1-辛烯的共聚研究[J].精细石油化工,2014, 31(3):80-83.