气相色谱-离子迁移谱应用于橄榄油的掺假鉴别
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摘要
建立气相离子迁移谱(gas chromatography-ion mobility spectrometry,GC-IMS)测定橄榄油等油类中可挥发组分的检测方法,样品在60℃条件孵化10 min通过顶空直接进GC-IMS进行分析,通过分析软件实验室分析浏览器(library analysis viewer,LAV)内置的主成分分析(principal component analysis,PCA)分析程序对数据进行处理。结果表明:建立的气相离子迁移谱检测方法可以对橄榄油等植物油中挥发组分进行检测分析;通过PCA主成分分析可以将特级初榨橄榄油与其他油类(果渣油和其他植物油)进行分离,并可以对果渣油、玉米胚芽油、葵花籽油、精炼棕榈油、花生油和大豆油的掺假进行判别分析,掺假鉴别率可以低至5%。
A gas chromatography-ion mobility spectrometry(GC-IMS) method was established for the determination of volatile components in olive oil. The procedure involves direct introduction of the olive oil samples into vial,headspace generation(incubation at 60 ℃ for 10 min),and automatic injection of the volatiles into a gas chromatography-ion mobility spectrometer. The data obtained after the analysis of olive oil and other vegetable oil were preprocessed and submitted to the built-in principal component analysis(PCA)program of the software library analysis viewer(LAV). A headspace device coupled to multi-capillary columnion mobility spectrometry had good performance in detecting volatiles in olive oils. The results from PCA showed extra virgin olive oil could be separated to other oils(pomace oil and other vegetable oils)and,adulteration of extra olive oil with pomace oil or corn oil or sunflower oil or refined palm oil or peanut oil or soybean oil was distinguished using PCA statistical analysis. The model was able to classify 100 % adulteration samples,and detected as low as 5 % of adulteration of extra olive oil with pomace oil,corn oil,sunflower oil,refined palm oil,peanut oil,soybean oil.
A gas chromatography-ion mobility spectrometry(GC-IMS) method was established for the determination of volatile components in olive oil. The procedure involves direct introduction of the olive oil samples into vial,headspace generation(incubation at 60 ℃ for 10 min),and automatic injection of the volatiles into a gas chromatography-ion mobility spectrometer. The data obtained after the analysis of olive oil and other vegetable oil were preprocessed and submitted to the built-in principal component analysis(PCA)program of the software library analysis viewer(LAV). A headspace device coupled to multi-capillary columnion mobility spectrometry had good performance in detecting volatiles in olive oils. The results from PCA showed extra virgin olive oil could be separated to other oils(pomace oil and other vegetable oils)and,adulteration of extra olive oil with pomace oil or corn oil or sunflower oil or refined palm oil or peanut oil or soybean oil was distinguished using PCA statistical analysis. The model was able to classify 100 % adulteration samples,and detected as low as 5 % of adulteration of extra olive oil with pomace oil,corn oil,sunflower oil,refined palm oil,peanut oil,soybean oil.
引文
[1]FLORES G,CASTILLO M L R,BLANCH G P et al.Detection of the adulteration of olive oils by solid phase microextraction and multidimentional gas chromatography[J].Food Chem,2006,97(2):336-342
[2]DIONISI F,PRODOLLIET J,TAGLIAFERRI E,et al.Assessment of olive oil adulteration by reversed-phase high-performance liquid chromatography/amperometric detection of tocopherols and tocotrienols[J].J Am Oil Chem Soc,1995,75(12):1505-1511
[3]赵延胜,董英,王子君,等.近红外光谱和气相色谱-质谱联用技术对橄榄油掺杂剂品质分析研究[J].现代科学仪器,2012(5):105-108
[4]JAFARI M,KADIVAR M,KERAMAT J,et al.Detection of adulteration in iranian olive oils using instrumental(GC.NMR,DSC)method[J].J Am Oil Chem Soc,2009,86(2):103-110
[5]LAI H,GUERRA P,JOSHI M,et al.Analysis of volatile components of drugs and explosives by solid phase microextraction-ion mobility spectrometry[J].J Sep Sci,2008,31(2):402-412
[6]ECKERS C,LAURES A M,Giles K,et al.Evaluating the utility of ion mobility separation in combination with high-pressure liquid chromatography/mass spectrometry to facilitate detection of trace impurities in formulated drug products[J].Rapid Comm Mass Spectrom,2007,21(7):1255-1263
[7]JUNGER M,BODEKER B,BAUMBACH J I.Peak assignment in multi-capillary column-ion mobility spectrometry using comparative studies with gas chromatography-mass spectrometry for VOC analysis[J].Anal Bioanal Chem,2010,396(1):471-482
[8]BAUMBACH J I.Process analysis using ion mobility spectrometry[J].Anal Bioanal Chem,2006,384(5):1059-1070
[9]GARRIDODELGADO R,DOBAOPRIETO M M,ARCE L,et al.Determination of volatile compounds by GC-IMS to assign the quality of virgin olive oil[J].Food Chem,2015,187:572-579
[10]GARRIDO-DELGADO R,MERCADER-TREJO F,SIELEMANN S,et al.Direct classification of olive oils by using two types of ion mobility spectrometers[J].Anal Chim Acta,2011,696(1/2):108-115
[11]GARRIDO-DELGADO R,ARCE L,VALC RCEL M.Multi-capillary column-ion mobility spectrometry:a potential screening system to differentiate virgin olive oils[J].Anal Bioanal Chem,2012,402(1):489-498
[12]GARRIDO-DELGADO M,DOBAO-PRIETO M,ARCE L,et al.Ion Mobility Spectrometry versus Classical Physico-chemical Analysis for Assessing the Shelf Life of Extra Virgin Olive Oil According to Container Type and Storage Conditions and Miguel Valca觃rce[J].Agricultural and food chemistry,2015,63(8):2179-2188
[2]DIONISI F,PRODOLLIET J,TAGLIAFERRI E,et al.Assessment of olive oil adulteration by reversed-phase high-performance liquid chromatography/amperometric detection of tocopherols and tocotrienols[J].J Am Oil Chem Soc,1995,75(12):1505-1511
[3]赵延胜,董英,王子君,等.近红外光谱和气相色谱-质谱联用技术对橄榄油掺杂剂品质分析研究[J].现代科学仪器,2012(5):105-108
[4]JAFARI M,KADIVAR M,KERAMAT J,et al.Detection of adulteration in iranian olive oils using instrumental(GC.NMR,DSC)method[J].J Am Oil Chem Soc,2009,86(2):103-110
[5]LAI H,GUERRA P,JOSHI M,et al.Analysis of volatile components of drugs and explosives by solid phase microextraction-ion mobility spectrometry[J].J Sep Sci,2008,31(2):402-412
[6]ECKERS C,LAURES A M,Giles K,et al.Evaluating the utility of ion mobility separation in combination with high-pressure liquid chromatography/mass spectrometry to facilitate detection of trace impurities in formulated drug products[J].Rapid Comm Mass Spectrom,2007,21(7):1255-1263
[7]JUNGER M,BODEKER B,BAUMBACH J I.Peak assignment in multi-capillary column-ion mobility spectrometry using comparative studies with gas chromatography-mass spectrometry for VOC analysis[J].Anal Bioanal Chem,2010,396(1):471-482
[8]BAUMBACH J I.Process analysis using ion mobility spectrometry[J].Anal Bioanal Chem,2006,384(5):1059-1070
[9]GARRIDODELGADO R,DOBAOPRIETO M M,ARCE L,et al.Determination of volatile compounds by GC-IMS to assign the quality of virgin olive oil[J].Food Chem,2015,187:572-579
[10]GARRIDO-DELGADO R,MERCADER-TREJO F,SIELEMANN S,et al.Direct classification of olive oils by using two types of ion mobility spectrometers[J].Anal Chim Acta,2011,696(1/2):108-115
[11]GARRIDO-DELGADO R,ARCE L,VALC RCEL M.Multi-capillary column-ion mobility spectrometry:a potential screening system to differentiate virgin olive oils[J].Anal Bioanal Chem,2012,402(1):489-498
[12]GARRIDO-DELGADO M,DOBAO-PRIETO M,ARCE L,et al.Ion Mobility Spectrometry versus Classical Physico-chemical Analysis for Assessing the Shelf Life of Extra Virgin Olive Oil According to Container Type and Storage Conditions and Miguel Valca觃rce[J].Agricultural and food chemistry,2015,63(8):2179-2188