短波紫外线处理对紫背天葵采后贮藏品质的影响
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摘要
为了减少紫背天葵的采后损失,提高其贮藏品质,采用不同辐照剂量的短波紫外线(UV-C)对采后紫背天葵进行处理,研究短波紫外线对紫背天葵采后品质及活性氧代谢相关酶的影响。结果表明,UV-C处理可以有效降低紫背天葵的失重率和菌落总数,延缓维生素C、叶绿素以及L*值的下降,减少总酚和黄酮的损失,抑制丙二醛(MDA)的积累,能较好地保证紫背天葵的感官品质和营养价值,同时显著提高超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等活性氧代谢相关酶的活性,其中5 k J·m~(-2)UV-C处理效果优于1、3 k J·m~(-2)UV-C处理组。本研究中UV-C处理能有效地提高紫背天葵的采后品质,其在采后紫背天葵贮藏保鲜中具有潜在的应用价值。
In order to reduce the post-harvest losses of Gynura bicolor and improve its post-harvest quality,the effect of different levels of short wave ultraviolet( UV-C) irradiation on the post-harvest quality and reactive oxygen species( ROS) metabolism-related enzyme activities of Gynura bicolor were analyzed in this study. The results showed that: UVC treatment can effectively reduce the weight loss and the total bacterial counts,delay the reduction of visual quality( L*value),vitamin C,chlorophyll,total phenolic and flavonoid contents,and inhibit the accumulation of MDA content. The sensory quality and nutritional quality of G. bicolor were effectively improved by UV-C irradiation treatment,and the activities of ROS metabolism-related enzymes( SOD,POD,CAT) were significantly increased. The effect of 5 k J·m~(-2) UV-C irradiation treatment was better than that of 1 or 3 k J·m~(-2) treatment groups. Results indicated that the UV-C treatment can effectively improve the post-harvest quality of G. bicolor,and had potential applications for G. bicolor preservation.
In order to reduce the post-harvest losses of Gynura bicolor and improve its post-harvest quality,the effect of different levels of short wave ultraviolet( UV-C) irradiation on the post-harvest quality and reactive oxygen species( ROS) metabolism-related enzyme activities of Gynura bicolor were analyzed in this study. The results showed that: UVC treatment can effectively reduce the weight loss and the total bacterial counts,delay the reduction of visual quality( L*value),vitamin C,chlorophyll,total phenolic and flavonoid contents,and inhibit the accumulation of MDA content. The sensory quality and nutritional quality of G. bicolor were effectively improved by UV-C irradiation treatment,and the activities of ROS metabolism-related enzymes( SOD,POD,CAT) were significantly increased. The effect of 5 k J·m~(-2) UV-C irradiation treatment was better than that of 1 or 3 k J·m~(-2) treatment groups. Results indicated that the UV-C treatment can effectively improve the post-harvest quality of G. bicolor,and had potential applications for G. bicolor preservation.
引文
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[6]李雪梅,张利红,马莲菊,陈强,王兰兰.不同UV-C辐射时间对豌豆幼苗光合特性及抗氧化酶活性的影响[J].生态与农村环境学报,2006,22(1):34-37
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[14]曹建康,姜微波.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007
[15]方华英,冯小霓.用丙酮提取液直接分光光度法测定绿茶中叶绿素的含量[J].福建茶叶,1982(1):14-17
[16]李巨秀,王柏玉.福林-酚比色法测定桑椹中总多酚[J].食品科学,2009,30(18):292-295
[17]陈华珍,陈建伟.费菜不同器官中总黄酮的含量比较及其薄层鉴别[J].中华中医药学刊,2003,21(11):1867-1867
[18]中国疾病预防控制中心营养与食品安全所.GB 4789.2-2010食品微生物学检验:菌落总数测定[S].北京:中国标准出版社,2010
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[20]Costa L,Vicente A R,Civello P M.UV-C treatment delays postharvest senescence in broccoli florets[J].Postharvest Biology&Technology,2006,39(2):204-210
[21]Vicente A R,Pineda C,Lemoine L.UV-C treatments reduce decay,retain quality and alleviate chilling injury in pepper[J].Postharvest Biology&Technology,2005,35(1):69-78
[22]Francisco A,Vi Ctorhugo E,Pedroantonio R.Effect of UV-Cradiation on quality of minimally processed spinach leaves[J].Journal of the Science of Food&Agriculture,2010,89(3):414-421
[23]马素娟.短波紫外处理对口蘑采后贮藏品质的影响[J].北方园艺,2015(2):118-121
[24]梁敏华,雷建敏,邵佳蓉,苏新国,杨震峰,陈伟.UV-C处理对桃果实酚类物质代谢和贮藏品质的影响[J].核农学报,2015,29(6):1088-1093
[25]尹明安,李玉娟,任小林.低剂量短波紫外线照射提高采后苹果抗病性[J].农业工程学报,2015,31(2):324-332
[26]陈少裕.膜脂过氧化对植物细胞的伤害[J].植物生理学报,1991(2):84-90
[27]姜天甲,陆仙英,蒋振晖,裴明黎,应铁进.短波紫外线处理对香菇采后品质的影响[J].农业机械学报,2010,41(2):108-112
[28]Bowler C,And MⅤM,Inze D.Superoxide dismutase and stress tolerance[J].Annual Review of Plant Biology,2003,43(1):83-116
[29]罗淑芬,孙凤杰,胡花丽,周宏胜,张雷刚,李鹏霞.6-苄基腺嘌呤对鲜莲子活性氧代谢及能量水平的影响[J].核农学报,2018,32(5):916-923
[30]陈晟,施木田,吴宇芬,李永裕.硼胁迫对不同类型西瓜活性氧代谢与抗氧化系统的影响[J].中国农学通报,2018,34(4):60-64
[31]Pombo M A,Rosli H G,Martínez G A.UV-C treatment affects the expression and activity of defense genes in strawberry fruit(Fragaria×ananassa,Duch.)[J].Postharvest Biology and Technology,2011,59(1):94-102
[32]郑杨,曹敏,申琳,生吉萍.短波紫外线照射对韭菜采后贮藏品质及活性氧代谢相关酶的影响[J].食品科学,2011,32(20):307-311
[33]Barka E A.Protective enzymes against reactive oxygen species during ripening of tomato(Lycopersicon esculentum)fruits in response to low amounts of UV-C[J].Functional Plant Biology,2001,28(8):785-791
[2]张林和,屠春燕,于文涛,武红丽.紫背天葵中营养成分及总黄酮分析[J].氨基酸和生物资源,2004,26(3):3-5
[3]Allende A,Artes F.UV-C radiation as a novel technique for keeping quality of fresh processed‘Lollo Rosso’lettuce[J].Food Research International,2003,36(7):739-746
[4]Alexandre E M C,Brand2o T R S,Silva C L M.Emerging technologies to improve the safety and quality of fruits and vegetables[J].Novel Technologies in Food Science,2012,7:261-297
[5]胡丽娜,张春岭,刘慧,吕珍珍,陈大磊.短波紫外线处理对采后山楂果营养品质及其抗氧化活性的影响[J].食品工业科技,2016,37(1):342-346
[6]李雪梅,张利红,马莲菊,陈强,王兰兰.不同UV-C辐射时间对豌豆幼苗光合特性及抗氧化酶活性的影响[J].生态与农村环境学报,2006,22(1):34-37
[7]陆仙英.短波紫外线诱导采后香梨品质与抗氧化体系变化及其生理机制[D].杭州:浙江大学,2013
[8]余斌,南运有,孔娜娜,党仁美,拜占玲,刘娟.采后UV-C辐照对马铃薯干腐病的抑制效应及相关酶活性的影响[J].核农学报,2017,31(9):1730-1736
[9]喻譞,姜璐璐,王焕宇,金鹏,郑永华.UV-C处理对杨梅采后品质及苯丙烷类代谢的影响[J].食品科学,2015,36(12):255-259
[10]杨震峰,王吉如,王珊珊,陈伟.短波紫外线处理对采后草莓果实腐烂和抗氧化能力的影响[J].核农学报,2012,26(5):775-780
[11]李玉娟,尹明安,任小林.采后短波紫外线处理对苹果耐贮性和品质的影响[J].食品科学,2015,36(14):244-249
[12]姜丽,蒋娟,张丽,傅淋然,郁志芳.采后植酸处理对紫背天葵贮藏期间生理生化反应的影响[J].食品科学,2014,35(2):316-321
[13]孟宪军,姜爱丽,胡文忠,田密霞,刘程惠.箱式气调贮藏对采后蓝莓生理生化变化的影响[J].食品工业科技,2011,32(9):379-383
[14]曹建康,姜微波.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007
[15]方华英,冯小霓.用丙酮提取液直接分光光度法测定绿茶中叶绿素的含量[J].福建茶叶,1982(1):14-17
[16]李巨秀,王柏玉.福林-酚比色法测定桑椹中总多酚[J].食品科学,2009,30(18):292-295
[17]陈华珍,陈建伟.费菜不同器官中总黄酮的含量比较及其薄层鉴别[J].中华中医药学刊,2003,21(11):1867-1867
[18]中国疾病预防控制中心营养与食品安全所.GB 4789.2-2010食品微生物学检验:菌落总数测定[S].北京:中国标准出版社,2010
[19]胡琼英,狄洌.生物化学实验[M].北京:北京大学出版社,2007
[20]Costa L,Vicente A R,Civello P M.UV-C treatment delays postharvest senescence in broccoli florets[J].Postharvest Biology&Technology,2006,39(2):204-210
[21]Vicente A R,Pineda C,Lemoine L.UV-C treatments reduce decay,retain quality and alleviate chilling injury in pepper[J].Postharvest Biology&Technology,2005,35(1):69-78
[22]Francisco A,Vi Ctorhugo E,Pedroantonio R.Effect of UV-Cradiation on quality of minimally processed spinach leaves[J].Journal of the Science of Food&Agriculture,2010,89(3):414-421
[23]马素娟.短波紫外处理对口蘑采后贮藏品质的影响[J].北方园艺,2015(2):118-121
[24]梁敏华,雷建敏,邵佳蓉,苏新国,杨震峰,陈伟.UV-C处理对桃果实酚类物质代谢和贮藏品质的影响[J].核农学报,2015,29(6):1088-1093
[25]尹明安,李玉娟,任小林.低剂量短波紫外线照射提高采后苹果抗病性[J].农业工程学报,2015,31(2):324-332
[26]陈少裕.膜脂过氧化对植物细胞的伤害[J].植物生理学报,1991(2):84-90
[27]姜天甲,陆仙英,蒋振晖,裴明黎,应铁进.短波紫外线处理对香菇采后品质的影响[J].农业机械学报,2010,41(2):108-112
[28]Bowler C,And MⅤM,Inze D.Superoxide dismutase and stress tolerance[J].Annual Review of Plant Biology,2003,43(1):83-116
[29]罗淑芬,孙凤杰,胡花丽,周宏胜,张雷刚,李鹏霞.6-苄基腺嘌呤对鲜莲子活性氧代谢及能量水平的影响[J].核农学报,2018,32(5):916-923
[30]陈晟,施木田,吴宇芬,李永裕.硼胁迫对不同类型西瓜活性氧代谢与抗氧化系统的影响[J].中国农学通报,2018,34(4):60-64
[31]Pombo M A,Rosli H G,Martínez G A.UV-C treatment affects the expression and activity of defense genes in strawberry fruit(Fragaria×ananassa,Duch.)[J].Postharvest Biology and Technology,2011,59(1):94-102
[32]郑杨,曹敏,申琳,生吉萍.短波紫外线照射对韭菜采后贮藏品质及活性氧代谢相关酶的影响[J].食品科学,2011,32(20):307-311
[33]Barka E A.Protective enzymes against reactive oxygen species during ripening of tomato(Lycopersicon esculentum)fruits in response to low amounts of UV-C[J].Functional Plant Biology,2001,28(8):785-791
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