矢车菊素与矢车菊素-3-O-葡萄糖苷对H2O2诱导HepG-2细胞氧化应激保护作用的比较
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摘要
以人肝癌细胞HepG-2为模型,以细胞抗氧化水平为指标,比较研究一种花色苷元矢车菊素(Cy)和其对应的花色苷矢车菊素-3-O-葡萄糖苷(C3G)对H_2O_2诱导的细胞氧化应激的保护作用。采用噻唑蓝(MTT)法检测细胞的存活率,采用总抗氧化能力(T-AOC)和细胞中的超氧化物歧化酶(SOD)活力、谷胱甘肽(GSH)及丙二醛(MDA)的含量评价样品的抗氧化保护作用。结果表明:两种花青素在0~0.5 mmol/L范围内对HepG-2细胞无明显的毒性;与H_2O_2氧化应激模型组相比,在高(400μmol/L)、中(200μmol/L)、低(50μmol/L)3种浓度下两种结构的花青素处理均可显著提高细胞总抗氧化能力、超氧化物歧化酶活力和还原型谷胱甘肽含量,降低细胞内丙二醛含量,抗氧化能力与花青素浓度呈正相关性;而Cy的细胞抗氧化作用显著高于C3G。该研究结果为阐明花青素的构效关系提供了体外研究的新证据。
Protection effects of cyanidin( Cy) and its derivate cyanidin-3-O-glucoside( C3G) on cellular oxidative stress induced by H_2O_2 on HepG-2 cell line were compared.The cell viabilities were detected by thiazolyl blue tetrazolium bromide( MTT) assay and the antioxidant protection were evaluated by total antioxidant capacity( T-AOC),the activity of superoxide dismutase( SOD),concentration of glutathione(GSH) and malondialdehyde(MDA) in cell.Results showed that two anthocyanins had no significant toxic effects on cultured HepG-2 cells under the concentration range of 0-0. 5 mmol/L. Compared with H_2O_2 control group,both anthocyanins could increase the levels of T-AOC and SOD,concentration ofGSH,and decrease the concentration of MDA in HepG-2 cells at different concentration of 50,200 and 400 μmol/L. The antioxidant capacity positively correlated with the concentration of anthocyanins. The antioxidant effect of Cy was significantly higher than that of C3G. The study could provide a theoretical basis for further understanding the structure-activity relationship of anthocyanin in vivo.
Protection effects of cyanidin( Cy) and its derivate cyanidin-3-O-glucoside( C3G) on cellular oxidative stress induced by H_2O_2 on HepG-2 cell line were compared.The cell viabilities were detected by thiazolyl blue tetrazolium bromide( MTT) assay and the antioxidant protection were evaluated by total antioxidant capacity( T-AOC),the activity of superoxide dismutase( SOD),concentration of glutathione(GSH) and malondialdehyde(MDA) in cell.Results showed that two anthocyanins had no significant toxic effects on cultured HepG-2 cells under the concentration range of 0-0. 5 mmol/L. Compared with H_2O_2 control group,both anthocyanins could increase the levels of T-AOC and SOD,concentration ofGSH,and decrease the concentration of MDA in HepG-2 cells at different concentration of 50,200 and 400 μmol/L. The antioxidant capacity positively correlated with the concentration of anthocyanins. The antioxidant effect of Cy was significantly higher than that of C3G. The study could provide a theoretical basis for further understanding the structure-activity relationship of anthocyanin in vivo.
引文
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[2]NORDBERG J,ARNER E S.Reactive oxygen species,antioxidants,and the mammalian thioredoxin system[J].Free radical biology&medicine,2001,31(11):1287.
[3]FRANDSEN J R,NARAYANASAMY P.Neuroprotection through flavonoid:enhancement of the glyoxalase pathway[J].Redox biology,2017,14(C):465.
[4]NAEIMI A F,ALIZADEH M.Antioxidant properties of the flavonoid fisetin:an updated review of in vivo and in vitro studies[J].Trends in food science&technology,2017,70:34.
[5]柯增光,许伟,邵荣,等.蓖麻碱的超声波辅助提取及其抗氧化性能[J].南京工业大学学报(自然科学版),2015,37(1):14.
[6]PUSPITA S,CHRISTOFORA H W,DONDIN S,et al.Colour properties,stability,and free radical scavenging activity of jambolan(Syzygium cumini)fruit anthocyanins in a beverage model system:natural and copigmentedanthocyanins[J].Food chemistry,2012,132(4):1908.
[7]CHANG L Z,ZHONG J C,XUE S B,et al.Structure-activity relationships of anthocyanidin glycosylation[J].Molecular diversity,2014,18(3):687.
[8]HUANG W Y,LIU Y M,WANG J,et al.Anti-inflammatory effect of the blueberry anthocyanins malvidin-3-glucoside and malvidin-3-galactoside in endothelial cells[J].Molecules,2014,19(8):12827.
[9]PILACZYNSKA-SZCZESNIAK L,SKARPANSKA-STEINBORN A,DESKUR E,et al.The influence of chokeberry juice supplementation on the reduction of oxidative stress resulting from an incremental rowing ergometer exercise[J].International journal of sport nutrition&exercise metabolism,2005,15(1):48.
[10]程琤,刘超,贺炜,等.紫甘薯花青素的稳定性及抗氧化性研究[J].营养学报,2011,33(3):291.
[11]YAN F,CHEN Y,AZAT R,et al.Mulberry anthocyanin extract ameliorates oxidative damage in Hep G2 cells and prolongs the lifespan of caenorhabditis elegans through MAPK and Nrf2pathways[J].Oxidative medicine and cellular longevity,2017(1):1.
[12]CHEN Z,WANG C,PAN Y,et al.Hypoglycemic and hypolipidemic effects of anthocyanins extract from black soybean seed coat in high fat diet and streptozotocin-induced diabetic mice[J].Food&function,2017,doi:10.1039/c7fo00983f.
[13]SHAN Q,ZHENG Y,LU J,et al.Purple sweet potato color ameliorates kidney damage via inhibiting oxidative stress mediated NLRP3 inflammasome activation in high fat diet mice[J].Food&chemical toxicology,2014,69:339.
[14]CASEDAS G,LES F,GOMEZ-SERRANILLOS M P,et al.Anthocyanin profile,antioxidant activity and enzyme inhibiting properties of blueberry and cranberry juices:a comparative study[J].Food&function,2017,8(11):4187.
[15]MANACH C,WILLIAMSON G,MORAND C,et al.Bioavailability and bioefficacy of polyphenols in humans:review of 97bioavailability studies[J].The American journal of clinical nutrition,2005,81(1):230S.
[16]HIDALGO M,MARTIN-SANTAMARIA S,RECIO I,et al.Potential anti-inflammatory,anti-adhesive,anti/estrogenic,and angiotensin-converting enzyme inhibitory activities of anthocyanins and their gut metabolites[J].Genes&nutrition,2012,7(2):295.
[17]KALT W,MCDONALD J E,LIU Y,et al.Flavonoid metabolites in human urine during blueberry anthocyanin intake[J].Journal of agricultural&food chemistry,2017,65(8):1582.
[18]KALT W,LIU Y,MCDONALD J E,et al.Anthocyanin metabolites are abundant and persistent in human urine[J].Journal of agricultural&food chemistry,2014,62:3926.
[19]冯所兰,周淅赟,牛文慧,等.黑米麸皮花青素脂质体的稳定性研究[J].食品工业科技,2015,36(22):115.
[20]KANETO H,KAWAMORI D,NAKATANI Y,et al.Oxidative stress and the JNK pathway as a potential therapeutic target for diabetes[J].Drug news&perspectives,2004,17(7):447.