蛋白质组学在IgA肾病的中西医研究进展
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摘要
目的探讨蛋白质组学在IgA肾病的中西医研究最新进展。方法通过对近年来国内外文献搜集、整理蛋白质组学在IgA肾病的研究概况。结果发现a1-抗胰蛋白酶、血浆铜蓝蛋白、粘连蛋白LG3、自由K轻链和a-辅肌动蛋白4等蛋白可能是诊断IgA肾病及预后评估的潜在标志物。M/Z为2798. 06以及M/Z为8713. 48的蛋白峰可能是IgA肾病瘀血证的标志物; M/Z为4987. 92蛋白峰可能是IgA肾病湿热证的标志物、M/Z为1972. 32蛋白峰可能是IgA肾病气阴两虚证的标志物。结论蛋白组学为IgA肾病的诊断、预后及证候分析提供了证据,该项技术值得进一步研究。
Objective To discuss the latest progress of proteomics technology in traditional chinese medicine and western medicine of IgA nephrology(IgAN). Methods Study the general application of proteomics technology in nephrology in recent years through domestic and foreign literature collection,sorting and induction methods. Results Using proteomics technology,several potential biomarkers have been identified for the diagnostic and prognostic evaluation of IgAN,including alpha-1-antitrypsin,ceruloplasmin,laminin G-like 3,free kappa light chains,α-actinin-4,etc. The protein peaks with mass-to-charge ratio of2798. 06、8713. 48 could be used for diagnosing IgA nephropathy with blood stasis syndrome. The protein peaks with mass-to-charge ratio of 4987. 92 could be used for diagnosing IgA nephropathy with damp-heat syndrome. The protein peaks with mass-to-charge ratio of 1972. 32 could be used for diagnosing IgA nephropathy with Qi and Yin deficiency syndrome. Conclusion Proteomics technology provides evidence for different diagnosis of IgA nephropathy. We should pay more attention to the research of proteomics technology.
Objective To discuss the latest progress of proteomics technology in traditional chinese medicine and western medicine of IgA nephrology(IgAN). Methods Study the general application of proteomics technology in nephrology in recent years through domestic and foreign literature collection,sorting and induction methods. Results Using proteomics technology,several potential biomarkers have been identified for the diagnostic and prognostic evaluation of IgAN,including alpha-1-antitrypsin,ceruloplasmin,laminin G-like 3,free kappa light chains,α-actinin-4,etc. The protein peaks with mass-to-charge ratio of2798. 06、8713. 48 could be used for diagnosing IgA nephropathy with blood stasis syndrome. The protein peaks with mass-to-charge ratio of 4987. 92 could be used for diagnosing IgA nephropathy with damp-heat syndrome. The protein peaks with mass-to-charge ratio of 1972. 32 could be used for diagnosing IgA nephropathy with Qi and Yin deficiency syndrome. Conclusion Proteomics technology provides evidence for different diagnosis of IgA nephropathy. We should pay more attention to the research of proteomics technology.
引文
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[23]张和韡.基于药物反证的Ig A肾病血瘀证血蛋白组学研究[D].福建中医药大学,2013.
[24]黄荣清,孙晓东,李艳玲,等.水蛭的研究进展[J].中西医结合学报,2004,2(5):387.
[25]贵艳丽,董德贤,李荣秀.日本医蛭中一种新抗凝血蛋白质的仿生亲和纯化及鉴定[J].中国生化药物杂志,2008,(03):145.
[26]王新荣,万廷信,赵著华,王文革,李银霞,杨百泉,李建忠,路新强.Ig A肾病中医湿热证微观辨证病理指标研究[J].中国中医药信息杂志,2015,22(08):31.
[27]王丽萍,杜建,庄永泽,等. Ig A肾病患者中医证候调查[J].福建中医药,2010,41(3):1.
[28]陈香美,陈以平,李平,等. 1016例Ig A肾病患者中医证候的多中心流行病学调查及相关因素分析[J].中国中西医结合杂志,2006,3(26):197.
[29]刘垠浩,王丽萍. Ig A肾病湿热证的血清蛋白质组学研究[J].中医临床研究,2014,6(14):7.
[30]王冬,于婷婷.浅谈糖皮质激素与相火学说的关系[J].中医药信息,2012,29(4):6.
[31]刘芬芬,羊维,黄琳,等.中医学对糖皮质激素主治功效的药性认识[J].中华中医药杂志,2015,30(4):1 268.
[32]程小燕,李顺民.比较分析气阴两虚型Ig A肾病患者与正常人肾系膜细胞的蛋白质组.新中医,2010,42(8):41.
[33]王晴. Ig A肾病气阴两虚证的血清蛋白组学研究[D].福建中医药大学,2014.
[2]柴可夫,马超,王宏献.早期糖尿病肾病不同证候的尿蛋白组学研究[J].中华中医药学刊,2015,33(10):2311.
[3]陈红谨,刘小溪,连捷,杨宇峰,石岩.中药益糖康治疗糖尿病大鼠的蛋白组学研究[J].中华中医药学刊,2017,35(02):477.
[4] Skates S,Iliopoulos O,Molecular markers for early detection of renalc arinoma:investigative approach[J]. Clin Caneer Res,2004,10(18Pt2):6296.
[5]李飞.筛选膀胱癌尿液新标记物Gc-globulin的荧光差异蛋白质组学研究[D].南方医科大学,2013.
[6] Wasinger VC,Cordwell SJ,Cerpa-Poljak A,et al. Progresswith gene product mapping of the Moll cutes:Mycoplasma genitalium[J]. Electrophoresis,995,16(7):1090.
[7] Cooks RG,Ouyang Z,Takats Z,et al. Detection Technologies. Ambient mass spectrometry[J]. Science,2006,311(5767):1566.
[8] Ru QC,Zhu LA,Katenhusen RA,etal. Exploring human plasma proteome strategies:high efficiency insolution digestion protocolfor multidimensional protein identification technology[J]. J Chromatogr A,2006,1111(2):175.
[9] Carvalho PC,Carvalho Mda G,Degrave W,et al. Differential protein expression patterns obtained by mass spectrometry canaid in the diagnosis of Hodgkin's disease[J]. J Exp Theroncol,2007,6(2):137.
[10] Prikryl P,Vojtova L,Maixnerova D,et al. Proteomics approach for identification of Ig A nephropathy-related biomarkers in urine.[J].Physiol Res,2017.
[11] Moon PG,Lee JE,You S,et al. Proteomic analysis of urinary exosomes from patients of early Ig A nephropathy and thin basement membrane nephropathy[J]. Proteomics,2011,11(12):2459.
[12] Surin B,Sachon E,Rougier JP,et al. LG3 fragment of endorepellin is a possible biomarker of severity in Ig A nephropathy[J]. Proteomics2013; 13:142.
[13] Rocchetti MT,Papale M,d'Apollo AM,Suriano IV,Di Palma AM,Vocino G,et al. Association of urinary laminin G-like 3 and free K light chains with disease activity and histological injury in Ig A nephropathy[J]. Clin J Am Soc Nephrol,2013,8:1115.
[14] Graterol F,Navarro-Mu oz M,Ibernon M. etal. Poor histological lesions in Ig A nephropathy may be reflected in blood and urine peptide profiling[J]. BMC Nephrol,2013,14:82.
[15] Kaneshiro N,Xiang Y,Nagai K,et al. Comprehensive analysis of short peptides in sera from patients with Ig A nephropathy[J]. Rapid Commun. Mass Spectrom,2009,23(23):3720.
[16] Sui WG,Cui ZZ,Zhang RS,et al. Comparative proteomic analysis of renal tissue in Ig A nephropathy with i TRAQ quantitativeproteomics.[J]. Biome dical Reports,2014,Vol. 2(6),pp. 793.
[17] Kojima S,Koitabashi K,Iizuka N,et al. Proteomic analysis of whole glomeruli in patients with Ig A nephropathy using microsieving.[J].Am J Nephrol,2014,39(1):36.
[18]孟庆宏,王常松,傅晓晴,等.蛋白质组学技术引入中医证理论的可行性研究[J],中医学报,2011,26(2):170.
[19]申定珠,李家邦,蒋荣鑫,等.证候蛋白质组学与中医证候学相关性探讨.中国中西医结合杂志,2006,26(4):366.
[20] Karoui K,Hill GS,Karras A,Jacquot C,et a1. A clinicopathologic study of thrombotic microangiopathy in 1gA nephropathy[J]. J Am Soc Nephrol,2012,23(1):137.
[21]苏鋆玉. Ig A肾病血瘀证的血蛋白组学研究[D].福建中医药大学,2012.
[22]王丽萍,张和韡,苏鋆玉,张勇,赵猛,庄永泽,陈建. Ig A肾病血瘀证血清蛋白组学的初步研究[J].中国中西医结合肾病杂志,2013,14(12):1062.
[23]张和韡.基于药物反证的Ig A肾病血瘀证血蛋白组学研究[D].福建中医药大学,2013.
[24]黄荣清,孙晓东,李艳玲,等.水蛭的研究进展[J].中西医结合学报,2004,2(5):387.
[25]贵艳丽,董德贤,李荣秀.日本医蛭中一种新抗凝血蛋白质的仿生亲和纯化及鉴定[J].中国生化药物杂志,2008,(03):145.
[26]王新荣,万廷信,赵著华,王文革,李银霞,杨百泉,李建忠,路新强.Ig A肾病中医湿热证微观辨证病理指标研究[J].中国中医药信息杂志,2015,22(08):31.
[27]王丽萍,杜建,庄永泽,等. Ig A肾病患者中医证候调查[J].福建中医药,2010,41(3):1.
[28]陈香美,陈以平,李平,等. 1016例Ig A肾病患者中医证候的多中心流行病学调查及相关因素分析[J].中国中西医结合杂志,2006,3(26):197.
[29]刘垠浩,王丽萍. Ig A肾病湿热证的血清蛋白质组学研究[J].中医临床研究,2014,6(14):7.
[30]王冬,于婷婷.浅谈糖皮质激素与相火学说的关系[J].中医药信息,2012,29(4):6.
[31]刘芬芬,羊维,黄琳,等.中医学对糖皮质激素主治功效的药性认识[J].中华中医药杂志,2015,30(4):1 268.
[32]程小燕,李顺民.比较分析气阴两虚型Ig A肾病患者与正常人肾系膜细胞的蛋白质组.新中医,2010,42(8):41.
[33]王晴. Ig A肾病气阴两虚证的血清蛋白组学研究[D].福建中医药大学,2014.