2015-2017年北京市顺义区食源性疾病监测腹泻病例霍乱弧菌感染及病原学特征分析
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摘要
目的研究食源性疾病监测腹泻患者霍乱弧菌感染特点及病原学特征,为科学防控和临床治疗提供依据。方法从2015-2017年北京市顺义区腹泻监测病例的粪便标本中分离培养霍乱弧菌,针对分离菌株进行毒力基因聚合酶链式反应(PCR)检测、脉冲场凝胶电泳(PFGE)、基质辅助激光解析电离飞行时间质谱(MALDI-TOF MS)图谱分析以及抗生素敏感性分析。结果 2015-2017年,共收集1 105例腹泻患者,霍乱弧菌的检出率为0.54%(6/1 105),霍乱毒素基因ctxAB均为阴性,血清学分型为非O1/O139型,其中5株霍乱弧菌携带Ⅲ型分泌系统毒力基因。菌株PFGE聚类分析结果和MALDI-TOF MS图谱聚类结果具有较好的一致性。结论霍乱弧菌流行强度不高,但其感染和病原学监测工作应在食源性疾病监测体系中得到重视。
Objective To understand the infection status and etiological characteristics of Vibrio cholerae in patients with food-borne diseases in Shunyi district of Beijing, and provide evidence for effective prevention, control and treatment of V. cholera infection. Methods V. cholerae isolation and culture were conducted by using stool samples collected from diarrhea cases in surveillance from 2015 to 2017. PCR test of virulence genes, pulsed-field gel electrophoresis(PFGE), matrixassisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS) and antibiotic susceptibility analysis were carried out for the isolates of V. cholerae. Results A total of 1 105 diarrhea patients were surveyed in three years. The detection rate of V. cholerae was 0.54%(6/1 105). The detection results of cholera toxin genes ctxAB were negative.Serological typing indicated that all the strains were non-O1/O139. PCR indicated that 5 of the 6 V. cholerae strains were type III secretion system(T3 SS) gene positive. The clustering results of PFGE were consistent with the clustering results of MALDI-TOF MS. Conclusion It is necessary to attach importance to the infection and etiological surveillance of V. cholerae in food-borne disease surveillance system
Objective To understand the infection status and etiological characteristics of Vibrio cholerae in patients with food-borne diseases in Shunyi district of Beijing, and provide evidence for effective prevention, control and treatment of V. cholera infection. Methods V. cholerae isolation and culture were conducted by using stool samples collected from diarrhea cases in surveillance from 2015 to 2017. PCR test of virulence genes, pulsed-field gel electrophoresis(PFGE), matrixassisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS) and antibiotic susceptibility analysis were carried out for the isolates of V. cholerae. Results A total of 1 105 diarrhea patients were surveyed in three years. The detection rate of V. cholerae was 0.54%(6/1 105). The detection results of cholera toxin genes ctxAB were negative.Serological typing indicated that all the strains were non-O1/O139. PCR indicated that 5 of the 6 V. cholerae strains were type III secretion system(T3 SS) gene positive. The clustering results of PFGE were consistent with the clustering results of MALDI-TOF MS. Conclusion It is necessary to attach importance to the infection and etiological surveillance of V. cholerae in food-borne disease surveillance system
引文
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[15]周妍妍,杨伟明,李世荣,等.临床分离非O1/O139群霍乱弧菌毒力及耐药特征分析[J].疾病检测,2016,31(6):517-521.DOI:10.3784/j.issn.1003-9961.2016.06.017.Zhou YY,Yang WM,Li SR,et al.Characteristics of virulence genes distribution and antibiotic resistance of non-O1/O139 Vibrio cholera[J].Dis Surveill,2016,31(6):517-521.DOI:10.3784/j.issn.1003-9961.2016.06.017.
[16]徐秋琼,李柏生,余泳红,等.广州市一起非O1/O139群霍乱弧菌食物中毒分离株的病原特征分析[J].疾病监测,2017,32(2):149-152.DOI:10.3784/j.issn.1003-9961.2017.02.016.Xu QQ,Li BS,Yu YH,et al.Etiological characteristics of Vibrio cholerae non-O1/O139 strains isolated from a food poisoning in Guangdong[J].Dis Surveill,2017,32(2):149-152.DOI:10.3784/j.issn.1003-9961.2017.02.016.
[17]Liao F,Mo ZS,Chen ML,et al.Comparison and evaluation of the molecular typing methods for toxigenic Vibrio cholerae in southwest China[J].Front Microbiol,2018,9:905.DOI:10.3389/fmicb.2018.00905.
[18]Ottaviani D,Medici L,Talevi G,et al.Molecular characterization and drug susceptibility of non-O1/O139 V.cholerae strains of seafood,environmental and clinical origin,Italy[J].Food Microbiol,2018,72:82-88.DOI:10.1016/j.fm.2017.11.011.
[2]Hao YY,Wang YL,Bi ZW,et al.A case of non-O1/non-O139Vibrio cholerae septicemia and meningitis in a neonate[J].Int JInfect Dis,2015,35(1):117-119.DOI:10.1016/j.ijid.2015.05.004.
[3]Jiang F,Bi RR,Deng LH,et al.Virulence-associated genes and molecular characteristics of non-O1/non-O139 Vibrio cholerae isolated from hepatitis B cirrhosis patients in China[J].Int J Infect Dis,2018,74(1):117-122.DOI:10.1016/j.ijid.2018.06.021.
[4]Laviad-Shitrit S,Sharaby Y,Izhaki I,et al.Antimicrobial susceptibility of environmental non-O1/non-O139 Vibrio cholerae Isolates[J].Front Microbiol,2018,9(1):1726.DOI:10.3389/fmicb.2018.01726.
[5]Miller KA,Chaand M,Gregoire S,et al.Characterization of V.cholerae T3SS-dependent cytotoxicity in cultured intestinal epithelial cells[J].Cell Microbiol,2016,18(12):1857-1870.DOI:10.1111/cmi.12629.
[6]中华人民共和国卫生部.WS 271-2007感染性腹泻诊断标准[S].北京:人民卫生出版社,2007.Ministry of Health of the People's Republic of China.WS271-2007 Diagnostic criteria for infectious diarrhea[S].Beijing:People's Medical Publishing House,2007.
[7]Luo Y,Ye JL,Jin DZ,et al.Molecular analysis of non-O1/nonO139 Vibrio cholerae isolated from hospitalised patients in China[J].BMC Microbiol,2013,13:52.DOI:10.1186/1471-2180-13-52.
[8]徐建国,阚飙,张建中,等.现场细菌学[M].北京:科学出版社,2011.Xu JG,Kan B,Zhang JZ,et al.Field bacteriology[M].Beijing:Science Press,2011.
[9]Li Y,Zhang S,He M,et al.Prevalence and molecular characterization of Campylobacter spp.isolated from patients with diarrhea in Shunyi,Beijing[J].Front Microbiol,2018,9(1):52.DOI:10.3389/fmicb.2018.00052.
[10]闻艳红,彭华,徐代庆,等.2012-2016年北京市昌平区感染性腹泻病原菌检测结果分析[J].现代预防医学,2017,44(13):2444-2447,2452.Wen YH,Peng H,Xu DQ,et al.Surveillance of infectious diarrhoea pathogens in Changping district,Beijing,2012-2016[J].Mod Prev Med,2017,44(13):2444-2447,2452.
[11]朱美娟,李颖,王彦波.2013-2015年北京市顺义区947份感染性腹泻病原菌监测结果分析[J].现代预防医学,2017,44(4):734-737,754.Zhu MJ,Li Y,Wang YB.Analysis on the 947 pathogens in infectious diarrhea in Shunyi district in Beijing from 2013 to2015[J].Mod Prev Med,2017,44(4):734-737,754.
[12]张爽,李颖,张彦春,等.2015-2017年北京市顺义区腹泻病例副溶血弧菌流行特征与分子分型特征分析[J].疾病监测,2018,33(5):381-386.DOI:10.3784/j.issn.1003-9961.2018.05.008.Zhang S,Li Y,Zhang YC,et al.Epidemiological and molecular characteristics of Vibrio parahaemolyticus in Shunyi district of Beijing,2015-2017[J].Dis Surveill,2018,33(5):381-386.DOI:10.3784/j.issn.1003-9961.2018.05.008.
[13]Zhang ZK,Lai SJ,Yu JX,et al.Etiology of acute diarrhea in the elderly in China:A six-year observational study[J].PLoS One,2017,12(3):e0173881.DOI:10.1371/journal.pone.0173881.
[14]秦帅,段然,景怀琦,等.我国2010-2014年不同城乡类型地区细菌性腹泻病原谱的流行特征[J].中华流行病学杂志,2018,39(5):651-655.DOI:10.3760/cma.j.issn.0254-6450.2018.05.021.Qin S,Duan R,Jing HQ,et al.Etiology of bacterial diarrhea in large cities,mid-sized/small cities and rural areas of China[J].Chin J Epidemiol,2018,39(5):651-655.DOI:10.3760/cma.j.issn.0254-6450.2018.05.021.
[15]周妍妍,杨伟明,李世荣,等.临床分离非O1/O139群霍乱弧菌毒力及耐药特征分析[J].疾病检测,2016,31(6):517-521.DOI:10.3784/j.issn.1003-9961.2016.06.017.Zhou YY,Yang WM,Li SR,et al.Characteristics of virulence genes distribution and antibiotic resistance of non-O1/O139 Vibrio cholera[J].Dis Surveill,2016,31(6):517-521.DOI:10.3784/j.issn.1003-9961.2016.06.017.
[16]徐秋琼,李柏生,余泳红,等.广州市一起非O1/O139群霍乱弧菌食物中毒分离株的病原特征分析[J].疾病监测,2017,32(2):149-152.DOI:10.3784/j.issn.1003-9961.2017.02.016.Xu QQ,Li BS,Yu YH,et al.Etiological characteristics of Vibrio cholerae non-O1/O139 strains isolated from a food poisoning in Guangdong[J].Dis Surveill,2017,32(2):149-152.DOI:10.3784/j.issn.1003-9961.2017.02.016.
[17]Liao F,Mo ZS,Chen ML,et al.Comparison and evaluation of the molecular typing methods for toxigenic Vibrio cholerae in southwest China[J].Front Microbiol,2018,9:905.DOI:10.3389/fmicb.2018.00905.
[18]Ottaviani D,Medici L,Talevi G,et al.Molecular characterization and drug susceptibility of non-O1/O139 V.cholerae strains of seafood,environmental and clinical origin,Italy[J].Food Microbiol,2018,72:82-88.DOI:10.1016/j.fm.2017.11.011.