双酶法提取红松籽油及其抗氧化分析
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摘要
红松籽油中含有丰富的营养成分并具有多种生物功能,但其生物利用度差、附加值低,成为其发展瓶颈。以红松籽仁为原料并以提油率和皮诺敛酸的量为指标,经超声波处理,蜗牛酶水解单因素试验,并通过响应面试验对酶解条件进行优化。结果表明,红松籽油提取的优化工艺条件为:碱性蛋白酶加酶量2 364.00 U/g、温度51.00℃、时间3.00 h、pH 8.40;蜗牛酶加酶量为39.00 mg/(10 g松子乳)、温度44.00℃、时间1.00 h、pH 7.00、料水比1∶5(g/m L),在此条件下,红松籽油得率可以达到93.87%,皮诺敛酸的量为855.77 mg/(10 g松子乳),明显高于传统方法的研究结果。同时,在最优条件下,红松籽油自由基清除的IC50值为8.52 mg/mL,而且红松籽油的抗氧化活性高于紫苏籽油等其他植物油。
Korean pine seed oil has much nutrients and many biological functions,poor bioavailability and low added value has become its the bottleneck of development. The Korean pine seeds were used as raw materials,and the oil extraction rate and the amount of pinolenic acid(PLA)was used as indicators. Disposed on ultrasonic conditions,single-factor hydrolysis of snail enzymes and its hydrolysis conditions were optimized by response surface methodology. The results showed that optimal extraction conditions were obtained as follows:under3.00 h of hydrolysis using 2 364.00 U/g of alcalase at 51.00 ℃and pH 8.40 and then by 1.00 h of hydrolysis using 39.00 mg from 10 g pine milk of snail enzyme at 44.00 ℃ and pH 7.00 with a substrate to water ratio of1 ∶ 5(g/mL). Under the conditions,the yield of pine nut oil and the yield of PLA were reached 93.87 % and855.77 mg from 10 g pine milk,respectively,which were higher than traditional method results significantly.Meanwhile,under the optimal conditions,the IC50 value for free radical scavenging of Korean pine seed oil was8.52 mg/mL,which was higher than perilla seed oil and other vegetable oils.
Korean pine seed oil has much nutrients and many biological functions,poor bioavailability and low added value has become its the bottleneck of development. The Korean pine seeds were used as raw materials,and the oil extraction rate and the amount of pinolenic acid(PLA)was used as indicators. Disposed on ultrasonic conditions,single-factor hydrolysis of snail enzymes and its hydrolysis conditions were optimized by response surface methodology. The results showed that optimal extraction conditions were obtained as follows:under3.00 h of hydrolysis using 2 364.00 U/g of alcalase at 51.00 ℃and pH 8.40 and then by 1.00 h of hydrolysis using 39.00 mg from 10 g pine milk of snail enzyme at 44.00 ℃ and pH 7.00 with a substrate to water ratio of1 ∶ 5(g/mL). Under the conditions,the yield of pine nut oil and the yield of PLA were reached 93.87 % and855.77 mg from 10 g pine milk,respectively,which were higher than traditional method results significantly.Meanwhile,under the optimal conditions,the IC50 value for free radical scavenging of Korean pine seed oil was8.52 mg/mL,which was higher than perilla seed oil and other vegetable oils.
引文
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[22]Bao L J,Li H E.Optimization on aqueous enzymatic extraction conditions of Decaisnea fargesii seed oil by response surface methodology[J].Cereals&Oils,2017,111(13):43-51
[23]Pinelo M,Arnous A,Meyer A S.Upgrading of grape skins:Significance of plant cell-wall structural components and extraction techniques for phenol release[J].Trends in Food Science&Technology,2006,17(11):579-590
[24]Li H,Zhang Z,He D,et al.Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties,fatty acid composition and antioxidant activity[J].Food Sci Technol,2017,37(15):113-115
[25]郭兴凤,陈定刚,孙金全,等.水酶法提油技术概述[J].粮油加工,2007(5):70-72
[2]Asset G,Leroy A,Bauge E,et al.Effects of dietary maritime pine(Pinus pinaster)-seed oil on high-density lipoprotein levels and in vitro cholesterol efflux in mice expressing human apolipoprotein A-I.[J].British Journal of Nutrition,2000,84(3):353-382
[3]Ferramosca A,Savy V,Einerhand A W C,et al.Pinus koraiensis seed oil(Pinno ThinTM)supplementation reduces body weight gain and lipid concentration in liver and plasma of mice[J].Journal of Animal&Feed Sciences,2008,17(4):621-630
[4]No d S,Zhao T T,Kim Y,et al.Preparation of highly purified pinolenic acid from pine nut oil using a combination of enzymatic esterification and urea complexation[J].Food Chemistry,2015,17(6):386-393
[5]王延群.营养干果话松子[J].山西老年,2016(4):58-58
[6]Cesarettin A,Ebru P.Fat-soluble bioactives in nuts[J].European Journal of Lipid Science&Technology,2011,113(8):943-949
[7]刘宏伟,王国义.红松果林营建与发展的几点建议[J].林业科技,2016,41(4):52-54
[8]杨建远,邓泽元.水酶法提取植物油脂技术研究进展[J].食品安全质量检测学报,2016,7(1):225-230
[9]程雪,张秀玲,孙瑞瑞,等.热处理辅助水酶法提取紫苏籽油的工艺优化[J].食品工业科技,2016,37(2):223-227
[10]李倩,赵丽,马媛,等.水酶法提取红花籽油工艺的研究[J].食品与发酵科技,2016,52(1):52-59
[11]朱婉华,宋东.蜗牛酶的研制及应用[J].中国生化药物杂志,1993(2):32-34
[12]Li Y,Jiang L,Wang.Optimization on Aqueous Enzymatic Extraction Conditions of Pine Seed Oil by Response Surface Method[J].Journal Chinese Cereals&Oils Association,2012,27(3):60-65
[13]包怡红,郭阳.响应面试验优化超声波辅助水酶法提取松籽油工艺及其氧化稳定性[J].食品科学,2016,37(22):60-68
[14]Yang Z,Zhai W.Identification and antioxidant activity of anthocyanins extracted from the seed and cob of purple corn(Zea mays L.)[J].Innovative Food Science&Emerging Technologies,2010,11(1):169-176
[15]Xiao L I,Chunyang L I,Zeng X,et al.Optimization of Aqueous Enzymatic Extraction of Safflower Oil by Response Surface Methodology[J].Journal of Anhui Agricultural Sciences,2017,34(2):137-143
[16]荣辉,吴兵兵,杨贤庆,等.响应面优化水酶法提取裂壶藻油的工艺[J].中国油脂,2018,43(2):112-114
[17]江连洲,王海晴,陈思,等.响应面法优化超声波辅助水酶法提取牡丹籽油工艺研究[J].食品工业科技,2016,37(8):247-251
[18]吴洪军,付婷婷,李靖彤,等.水代法提取红松籽油加工工艺的研究[J].中国林副特产,2014(4):31-33
[19]李鹏飞.水酶法提取花生油及蛋白质[D].无锡:江南大学,2017:17-35
[20]Yu-Jian L I,Chen F S,Hao L H,et al.Research advance in aqueous enzymatic extraction of peanut oil[J].Cereals&Oils,2017,12(4):54-61
[21]Yang Y C,Ji L,Zu Y G,et al.Optimisation of microwave-assisted enzymatic extraction of corilagin and geraniin from Geranium sibiricum Linne and evaluation of antioxidant activity[J].Food Chemistry,2010,122(1):373-380
[22]Bao L J,Li H E.Optimization on aqueous enzymatic extraction conditions of Decaisnea fargesii seed oil by response surface methodology[J].Cereals&Oils,2017,111(13):43-51
[23]Pinelo M,Arnous A,Meyer A S.Upgrading of grape skins:Significance of plant cell-wall structural components and extraction techniques for phenol release[J].Trends in Food Science&Technology,2006,17(11):579-590
[24]Li H,Zhang Z,He D,et al.Ultrasound-assisted aqueous enzymatic extraction of oil from perilla seeds and determination of its physicochemical properties,fatty acid composition and antioxidant activity[J].Food Sci Technol,2017,37(15):113-115
[25]郭兴凤,陈定刚,孙金全,等.水酶法提油技术概述[J].粮油加工,2007(5):70-72