外源氮、磷添加对琼北荔枝园土壤CH_4氧化能力的影响
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摘要
为明确氮(N)和磷(P)的输入对土壤氧化吸收大气甲烷(CH_4)的影响,通过室内培养实验,研究了外源N、P添加对琼北3种荔枝园土壤CH_4氧化速率的短期影响。结果表明:不同浓度处理的外源N[(NH4)2SO4]添加对3种荔枝园土壤(暗红湿润铁铝土,用T2表示;简育湿润铁铝土,用T3表示;湿润玻璃火山灰土,用H3表示)CH_4氧化速率均产生抑制作用(P<0.05),较对照组平均抑制率分别达到29.93%、36.28%和24.80%。不同浓度外源P(KH2PO4)添加对3种荔枝园土壤CH_4氧化具有促进作用,其中对T2、T3土壤CH_4氧化具有明显的促进作用,H3呈现先促进后抑制的变化趋势。外源N、P同时添加对3种土壤CH_4氧化均起到了促进作用,与单独添加外源N相比,N、P同时添加缓解了外源N对3种荔枝园土壤CH_4氧化能力的抑制作用,其中T2土壤的缓解作用最高,这可能与其土壤的速效磷背景值较低有关。因此,在荔枝园土壤的施肥管理中应根据土壤类型合理增施N、P肥,以促使土壤对大气CH_4的氧化能力维持在较高水平上。
Nitrogen(N)and phosphorus(P)inputs are important factors affecting atmospheric methane(CH_4)consumption by soils. Using laboratory incubation techniques, the short-term effects of N and P addition on soil CH_4 oxidation in different types of litchi orchard soils in northern Hainan Island were determined in July 2016. Further, the soil samples collected from three litchi orchards in northern Hainan island were incubated at 26 ℃ with gravimetric water content of 15% and an initial CH_4 concentration of approximately 15 μL·L-1 to determine the effects of exogenous N and P on methane oxidation in different types of soils. The results showed that CH_4 oxidation in all litchi orchard soils[Rhodi-Udic Ferralosols(T2), Hapli-Udic Ferralosols(T3), and Udi-Vitric Andosols(H3)] decreased significantly after the exogenous addition of N[(NH4)2 SO4]. Compared with that of the control treatment, CH_4 oxidation rate in different types of soils decreased by29.93%, 36.28%, and 24.80%, respectively. Methane oxidation in litchi garden soils(T2 and T3)significantly increased after the addition of exogenous P(KH2 PO4); CH_4 oxidation rates initially increased with incubation time but then decreased in H3 soils. The addition of N and P can stimulate CH_4 oxidation in three litchi soils. N and P were added to mitigate the inhibitory effect of exogenous N on the CH_4 oxidation capacity of three litchi orchard soils, and the relieving effect in T2 soil was higher, which might be attributed to the background value of available P in soil. Therefore, the selection of appropriate N and P fertilizers and the improvement of organic matter status of soils are recommended to maintain high CH_4 oxidation rates in upland soils.
Nitrogen(N)and phosphorus(P)inputs are important factors affecting atmospheric methane(CH_4)consumption by soils. Using laboratory incubation techniques, the short-term effects of N and P addition on soil CH_4 oxidation in different types of litchi orchard soils in northern Hainan Island were determined in July 2016. Further, the soil samples collected from three litchi orchards in northern Hainan island were incubated at 26 ℃ with gravimetric water content of 15% and an initial CH_4 concentration of approximately 15 μL·L-1 to determine the effects of exogenous N and P on methane oxidation in different types of soils. The results showed that CH_4 oxidation in all litchi orchard soils[Rhodi-Udic Ferralosols(T2), Hapli-Udic Ferralosols(T3), and Udi-Vitric Andosols(H3)] decreased significantly after the exogenous addition of N[(NH4)2 SO4]. Compared with that of the control treatment, CH_4 oxidation rate in different types of soils decreased by29.93%, 36.28%, and 24.80%, respectively. Methane oxidation in litchi garden soils(T2 and T3)significantly increased after the addition of exogenous P(KH2 PO4); CH_4 oxidation rates initially increased with incubation time but then decreased in H3 soils. The addition of N and P can stimulate CH_4 oxidation in three litchi soils. N and P were added to mitigate the inhibitory effect of exogenous N on the CH_4 oxidation capacity of three litchi orchard soils, and the relieving effect in T2 soil was higher, which might be attributed to the background value of available P in soil. Therefore, the selection of appropriate N and P fertilizers and the improvement of organic matter status of soils are recommended to maintain high CH_4 oxidation rates in upland soils.
引文
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[11]吴回军,欧阳学军.磷添加对南亚热带森林土壤有机碳氮矿化影响的培养试验研究[J].广东林业科技, 2008, 24(6):6-14.WU Hui-jun, OUYANG Xue-jun. Effects of P addition on organic C and N mineralization from subtropical forests soil during incubation,southern China[J]. Guangdong Forestry Science and Technology,2008, 24(6):6-14.
[12] Liu L, Gundersen P, Zhang T, et al. Effects of phosphorus addition on soil microbial biomass and community composition in three forest types in tropical China[J]. Soil Biology&Biochemistry, 2012, 44(1):31-38.
[13] Cleveland C C, Townsend A R, Schmidt S K. Phosphorus limitation of microbial processes in moist tropical forests:Evidence from shortterm laboratory incubations and field studies[J]. Ecosystems, 2002, 5(7):0680-0691.
[14] Ilstedt U, Singh S. Nitrogen and phosphorus limitations of microbial respiration in a tropical phosphorus-fixing acrisol(ultisol)compared with organic compost[J]. Soil Biology&Biochemistry, 2005, 37(7):1407-1410.
[15] Rowlings D W, Grace P R, Scheer C, et al. Influence of nitrogen fertilizer application and timing on greenhouse gas emissions from a lychee(Litchi chinensis)orchard in humid subtropical Australia[J]. Agriculture, Ecosystems and Environment, 2013, 179(4):168–178.
[16]孙娟,李松刚,魏志远,等.海南中西部妃子笑荔枝营养诊断初探[J].热带作物学报, 2014, 35(10):1932–1936.SUN Juan, LI SONG-gang, WEI Zhi-yuan, et al. The nutrient diagnosis of Feizhixiao litchi in Hainan[J]. Chinese Journal of Tropical Crops, 2014, 35(10):1932–1936.
[17]齐莎,赵小蓉,郑海霞,等.内蒙古典型草原连续5年施用氮磷肥土壤生物多样性的变化[J].生态学报, 2010, 30(20):5518-5526.QI Sha, ZHAO Xiao-rong, ZHENG Hai-xia, et al. Changes of soil biodiversity in Inner Mongolia steppe after 5 years of N and P fertilizer applications[J]. Acta Ecologica Sinica, 2010, 30(20):5518-5526.
[18]龚子同,张甘霖,漆智平.海南岛土系概论[M].北京:科学出版社,2004.GONG Zi-tong, ZHANG Gan-lin, QI Zhi-ping. Hainan island soil series introduction[M]. Beijing:Science Press, 2004.
[19]魏晋.不同区域森林土壤甲烷氧化及其影响因子的初步研究[D].南京:南京农业大学, 2007.WEI Jin. A pimary study on methane consumption by forest soils from different zones and its controlling factors[D]. Nanjing:Nanjing Agriculture University, 2007.
[20] Lima A B, Muniz A W, Dumont M G. Activity and abundance of methane-oxidizing bacteria in secondary forest and manioc plantations of Amazonian Dark Earth and their adjacent soils[J]. Frontiers in Microbiology, 2014, 5:550.
[21] Jang I, Lee S, Zoh K-D, et al. Methane concentrations and methanotrophic community structure influence the response of soil methane oxidation to nitrogen content in a temperate forest[J]. Soil Biology&Biochemistry, 2011, 43(3):620-627.
[22] Li X Y, Cheng S L, Fang H J, et al. The contrasting effects of deposited NH4+and NO-3on soil CO2, CH4 and N2O fluxes in a subtropical plantation, southern China[J]. Ecological Engineering, 2015, 85:317-327.
[23]王智平,胡春胜,杨居荣.无机氮对土壤甲烷氧化作用的影响[J].应用生态学报, 2003, 14(2):305-309.WANG Zhi-ping, HU Chun-sheng, YANG Ju-rong. Effect of inorganic nitrogen on the oxidation of methane in soil[J]. Chinese Journal of Applied Ecology, 2003, 14(2):305-309.
[24] Schnell S, King G M. Mechanistic analysis of ammonium inhibition of atmospheric methane consumption in forest soils[J]. Appl Environ Microbiol, 1994, 60(10):3514-3521.
[25]胡敏杰,仝川,邹芳芳.氮输入对土壤甲烷产生、氧化和传输过程的影响及其机制[J].草业学报, 2015, 24(6):204-212.HU Min-jie, TONG Chuan, ZOU Fang-fang. Effects of nitrogen input on CH4 production, oxidation and transport in soils, and mechanisms:A review[J]. Acta Prataculturae Sinica, 2015, 24(6):204-212.
[26]胡敏杰,邹芳芳,任鹏,等.闽江河口湿地土壤CH4产生与氧化速率对外源氮、硫添加的响应[J].生态学报, 2017, 37(1):167-176.HU Min-jie, ZOU Fang-fang, REN Peng, et al. Effects of nitrogen and sulfate additions on methane production and oxidation in the Min River estuarine marsh[J]. Acta Ecologica Sinica, 2017, 37(1):167-176.
[27]林先贵,胡君利.土壤微生物多样性的科学内涵及其生态服务功能[J].土壤学报, 2008, 45(5):892-900.LIN Xian-gui, HU Jun-li. Scientific connotation and ecological service function of soil microbial diversity[J]. Acta Pedologica Sinica,2008, 45(5):892-900.
[28] Liu L, Gundersen P, Zhang T, et al. Effects of phosphorus addition on soil microbial biomass and community composition in three forest types in tropical China[J]. Soil Biology&Biochemistry, 2012, 44(1):31-38.
[29] Jugnia L B, Mottiar Y, Djuikom E, et al. Effect of compost, nitrogen salts, and N P K fertilizers on methane oxidation potential at different temperatures[J]. Applied Microbiology&Biotechnology, 2012, 93(6):2633-2643.
[30]李霞,田光明,朱军,等.不同磷肥用量对水稻土有机碳矿化和细菌群落多样性的影响[J].土壤学报, 2014, 51(2):360-372.LI Xia, TIAN Guang-ming, ZHU Jun, et al. Effects of rate of phosphorus fertilizer on organic carbon mineralization and bacterial community diversity in paddy soil[J]. Acta Pedologica Sinica, 2014, 51(2):360-372.
[31] Tong C, Wang C, Huang J F, et al. Ecosystem respiration does not differ before and after tidal inundation in brackish marshes of the Min River estuary, southeast China[J]. Wetlands, 2014, 34(2):225-233.
[32]王维奇,曾从盛,仝川.闽江口芦苇湿地土壤甲烷产生与氧化能力研究[J].湿地科学, 2008, 6(1):60-68.WANG Wei-qi, ZENG Cong-sheng, TONG Chuan. Methane production and oxidation capacities of soil from the reed marsh of the Minjiang River estuary[J]. Wetland Science, 2008, 6(1):60-68.
[33] Tan H, Barret M, Mooij M J, et al. Long-term phosphorus fertilisation increased the diversity of the total bacterial community and the phod phosphorus mineraliser group in pasture soils[J]. Biology and Fertility of Soils, 2012, 49(6):1-12.
[34]刘剑秋,曾从盛,陈宁.闽江河口湿地研究[M].北京:科学出版社, 2006.LIU Jian-qiu, ZENG Cong-sheng, CHEN Ning. Study on wetland in Minjiang estuary[M]. Beijing:Science Press, 2006.
[35]齐润杰,陈金霞,但建国.外源氮对琼北不同类型土壤甲烷氧化能力的影响[J].热带作物学报, 2016, 37(8):1534-1539.QI Run-jie, CHEN Jin-xia, DAN Jian-guo. Effects of exogenous nitrogen on methane oxidation in upland soils of different types in northern Hainan island[J]. Chinese Journal of Tropical Crops, 2016, 37(8):1534-1539.
[2] Salawitch R J, Bennett B F, Hope A P, et al. Earth′s climate system[M]//Salawitch R J, Canty T P, Hope A P, et al. Paris climate agreement:Beacon of hope. Cham:Springer International Publishing AG,2017:1–50.
[3] World Meteorological Organization. The state of greenhouse gases in the atmosphere based on global observations through 2016[J]. WMO Greenhouse Gas Bulletin, 2017, 13:1–8.
[4] Agren G I. Stoichiometry and nutrition of plant growth in natural communities[J]. Annual Review of Ecology, Evolution,&Systematics, 2008,39:153-170.
[5] Maljanen M, Jokinen H, Saari A, et al. Methane and nitrous oxide fluxes, and carbon dioxide production in boreal forest soil fertilized with wood ash and nitrogen[J]. Soil Use&Management, 2006, 22(2):151–157.
[6]丁维新,蔡祖聪.土壤甲烷氧化菌及水分状况对其活性的影响[J].中国生态农业学报, 2003, 11(1):94-97.DING Wei-xin, CAI Zu-cong. Methanism of methane oxidation by methanotrophs and effect of soil moisture content on their activity[J].Chinese Academy of Science, 2003, 11(1):94-97.
[7] Shrestha R K, Strahm B D, Sucre E B. Greenhouse gas emissions in response to nitrogen fertilization in managed forest ecosystems[J]. New Forests, 2015, 46(2):167-193.
[8]方华军,程淑兰,于贵瑞,等.大气氮沉降对森林土壤甲烷吸收和氧化亚氮排放的影响及其微生物学机制[J].生态学报, 2014, 34(17):4799-4806.FANG Hua-jun, CHENG Shu-lan, YU Gui-rui, et al. Microbial mechanism responsible for the effects of atmospheric nitrogen deposition on methane uptake and nitrous oxide emission in forest soils:A review[J]Acta Ecologica Sinica, 2014, 34(17):4799-4806.
[9] Zhang T, Zhu W, Mo J, et al. Increased phosphorus availability mitigates the inhibition of nitrogen deposition on CH4 uptake in an oldgrowth tropical forest, southern China[J]. Biogeosciences, 2011, 8(9):2805-2813.
[10] Gray N D, Mccann C M, Christgen B, et al. Soil geochemistry confines microbial abundances across an arctic landscape;implications for net carbon exchange with the atmosphere[J]. Biogeochemistry, 2014, 120(1/2/3):307-317.
[11]吴回军,欧阳学军.磷添加对南亚热带森林土壤有机碳氮矿化影响的培养试验研究[J].广东林业科技, 2008, 24(6):6-14.WU Hui-jun, OUYANG Xue-jun. Effects of P addition on organic C and N mineralization from subtropical forests soil during incubation,southern China[J]. Guangdong Forestry Science and Technology,2008, 24(6):6-14.
[12] Liu L, Gundersen P, Zhang T, et al. Effects of phosphorus addition on soil microbial biomass and community composition in three forest types in tropical China[J]. Soil Biology&Biochemistry, 2012, 44(1):31-38.
[13] Cleveland C C, Townsend A R, Schmidt S K. Phosphorus limitation of microbial processes in moist tropical forests:Evidence from shortterm laboratory incubations and field studies[J]. Ecosystems, 2002, 5(7):0680-0691.
[14] Ilstedt U, Singh S. Nitrogen and phosphorus limitations of microbial respiration in a tropical phosphorus-fixing acrisol(ultisol)compared with organic compost[J]. Soil Biology&Biochemistry, 2005, 37(7):1407-1410.
[15] Rowlings D W, Grace P R, Scheer C, et al. Influence of nitrogen fertilizer application and timing on greenhouse gas emissions from a lychee(Litchi chinensis)orchard in humid subtropical Australia[J]. Agriculture, Ecosystems and Environment, 2013, 179(4):168–178.
[16]孙娟,李松刚,魏志远,等.海南中西部妃子笑荔枝营养诊断初探[J].热带作物学报, 2014, 35(10):1932–1936.SUN Juan, LI SONG-gang, WEI Zhi-yuan, et al. The nutrient diagnosis of Feizhixiao litchi in Hainan[J]. Chinese Journal of Tropical Crops, 2014, 35(10):1932–1936.
[17]齐莎,赵小蓉,郑海霞,等.内蒙古典型草原连续5年施用氮磷肥土壤生物多样性的变化[J].生态学报, 2010, 30(20):5518-5526.QI Sha, ZHAO Xiao-rong, ZHENG Hai-xia, et al. Changes of soil biodiversity in Inner Mongolia steppe after 5 years of N and P fertilizer applications[J]. Acta Ecologica Sinica, 2010, 30(20):5518-5526.
[18]龚子同,张甘霖,漆智平.海南岛土系概论[M].北京:科学出版社,2004.GONG Zi-tong, ZHANG Gan-lin, QI Zhi-ping. Hainan island soil series introduction[M]. Beijing:Science Press, 2004.
[19]魏晋.不同区域森林土壤甲烷氧化及其影响因子的初步研究[D].南京:南京农业大学, 2007.WEI Jin. A pimary study on methane consumption by forest soils from different zones and its controlling factors[D]. Nanjing:Nanjing Agriculture University, 2007.
[20] Lima A B, Muniz A W, Dumont M G. Activity and abundance of methane-oxidizing bacteria in secondary forest and manioc plantations of Amazonian Dark Earth and their adjacent soils[J]. Frontiers in Microbiology, 2014, 5:550.
[21] Jang I, Lee S, Zoh K-D, et al. Methane concentrations and methanotrophic community structure influence the response of soil methane oxidation to nitrogen content in a temperate forest[J]. Soil Biology&Biochemistry, 2011, 43(3):620-627.
[22] Li X Y, Cheng S L, Fang H J, et al. The contrasting effects of deposited NH4+and NO-3on soil CO2, CH4 and N2O fluxes in a subtropical plantation, southern China[J]. Ecological Engineering, 2015, 85:317-327.
[23]王智平,胡春胜,杨居荣.无机氮对土壤甲烷氧化作用的影响[J].应用生态学报, 2003, 14(2):305-309.WANG Zhi-ping, HU Chun-sheng, YANG Ju-rong. Effect of inorganic nitrogen on the oxidation of methane in soil[J]. Chinese Journal of Applied Ecology, 2003, 14(2):305-309.
[24] Schnell S, King G M. Mechanistic analysis of ammonium inhibition of atmospheric methane consumption in forest soils[J]. Appl Environ Microbiol, 1994, 60(10):3514-3521.
[25]胡敏杰,仝川,邹芳芳.氮输入对土壤甲烷产生、氧化和传输过程的影响及其机制[J].草业学报, 2015, 24(6):204-212.HU Min-jie, TONG Chuan, ZOU Fang-fang. Effects of nitrogen input on CH4 production, oxidation and transport in soils, and mechanisms:A review[J]. Acta Prataculturae Sinica, 2015, 24(6):204-212.
[26]胡敏杰,邹芳芳,任鹏,等.闽江河口湿地土壤CH4产生与氧化速率对外源氮、硫添加的响应[J].生态学报, 2017, 37(1):167-176.HU Min-jie, ZOU Fang-fang, REN Peng, et al. Effects of nitrogen and sulfate additions on methane production and oxidation in the Min River estuarine marsh[J]. Acta Ecologica Sinica, 2017, 37(1):167-176.
[27]林先贵,胡君利.土壤微生物多样性的科学内涵及其生态服务功能[J].土壤学报, 2008, 45(5):892-900.LIN Xian-gui, HU Jun-li. Scientific connotation and ecological service function of soil microbial diversity[J]. Acta Pedologica Sinica,2008, 45(5):892-900.
[28] Liu L, Gundersen P, Zhang T, et al. Effects of phosphorus addition on soil microbial biomass and community composition in three forest types in tropical China[J]. Soil Biology&Biochemistry, 2012, 44(1):31-38.
[29] Jugnia L B, Mottiar Y, Djuikom E, et al. Effect of compost, nitrogen salts, and N P K fertilizers on methane oxidation potential at different temperatures[J]. Applied Microbiology&Biotechnology, 2012, 93(6):2633-2643.
[30]李霞,田光明,朱军,等.不同磷肥用量对水稻土有机碳矿化和细菌群落多样性的影响[J].土壤学报, 2014, 51(2):360-372.LI Xia, TIAN Guang-ming, ZHU Jun, et al. Effects of rate of phosphorus fertilizer on organic carbon mineralization and bacterial community diversity in paddy soil[J]. Acta Pedologica Sinica, 2014, 51(2):360-372.
[31] Tong C, Wang C, Huang J F, et al. Ecosystem respiration does not differ before and after tidal inundation in brackish marshes of the Min River estuary, southeast China[J]. Wetlands, 2014, 34(2):225-233.
[32]王维奇,曾从盛,仝川.闽江口芦苇湿地土壤甲烷产生与氧化能力研究[J].湿地科学, 2008, 6(1):60-68.WANG Wei-qi, ZENG Cong-sheng, TONG Chuan. Methane production and oxidation capacities of soil from the reed marsh of the Minjiang River estuary[J]. Wetland Science, 2008, 6(1):60-68.
[33] Tan H, Barret M, Mooij M J, et al. Long-term phosphorus fertilisation increased the diversity of the total bacterial community and the phod phosphorus mineraliser group in pasture soils[J]. Biology and Fertility of Soils, 2012, 49(6):1-12.
[34]刘剑秋,曾从盛,陈宁.闽江河口湿地研究[M].北京:科学出版社, 2006.LIU Jian-qiu, ZENG Cong-sheng, CHEN Ning. Study on wetland in Minjiang estuary[M]. Beijing:Science Press, 2006.
[35]齐润杰,陈金霞,但建国.外源氮对琼北不同类型土壤甲烷氧化能力的影响[J].热带作物学报, 2016, 37(8):1534-1539.QI Run-jie, CHEN Jin-xia, DAN Jian-guo. Effects of exogenous nitrogen on methane oxidation in upland soils of different types in northern Hainan island[J]. Chinese Journal of Tropical Crops, 2016, 37(8):1534-1539.