利用MAS技术培育水稻多抗、优质强恢复系桂恢663
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摘要
【目的】培育新的兼抗稻瘟病、白叶枯病和细菌性条斑病,且米质较优的水稻强恢复系,为选育抗病、高产、优质的杂交稻组合提供良好的亲本材料。【方法】以抗稻瘟病品种合丰占为母本,与抗白叶枯病品系粤泰占杂交,通过分子标记辅助选择(MAS),获得多个抗病基因聚合的优良单株,再经过多代自交选育,并进行田间抗性及米质鉴定、农艺性状考察及恢复力测试。【结果】培育出聚合了抗稻瘟病基因Pi-ta、Pib、Pi54和抗白叶枯病兼抗细条病基因xa5的多抗、优质强恢复系桂恢663。桂恢663对白叶枯Ⅳ型菌和强毒Ⅴ型菌表现为抗,对广西细条病优势菌株JZ-8表现为抗,对14个稻瘟病菌株,有12个达到1级抗性。桂恢663除直链淀粉含量偏低外,其余6个主要米质指标均达到国标2级标准,其与丰田1A所配抗病、高产、优质组合丰田优663通过广西品种审定。桂恢663和丰田优663谷粒具有香味。【结论】桂恢663兼抗稻瘟病、白叶枯病和细条病,且恢复力强、米质较优具有香味,是组配抗病、高产、优质杂交组合的优良亲本,同时为水稻提供重要遗传基础材料。
【Objective】To provide outstanding parent for hybrid rice of disease-resistance, high-quality and high-yield, a strong rice restorer line with resistant to rice blast, bacterial blight, and rice bacterial leaf streak was breed.【Method】Material Hefengzhan with resistant to rice blast was receptor parent, and material Yuetaizhan with resistant to bacterial blight was donor parent. And excellent individuals were obtained with multiple disease-resistance genes by Molecular marker assisted selection technique. The individuals were selected for self intersection till they were stable. The resistance and rice quality were also detected. Agronomic traits and restoring ability were analyzed.【Result】A strong rice restorer line with resistant to rice blast genes Pi-ta,Pib and Pi54, bacterial blight and rice bacterial leaf streak genes xa5 was bred. It was named Guihui 663. Inoculations Guihui 663 with the predominant pathogenic types Ⅳ and types Ⅴof Xanthomonas oryzae pv. oryzae and representative types JZ-8 of rice bacterial leaf streak in Guangxi. The results showed resistance. Inoculations Guihui 663 with 14 rice blast, 12 of them identified resistance grade 1. The rice quality was identified as national quality grade 2 except amylose content. Guihui 663 crossed with sterile line Fengtian1 A and obtained hybrid rice combination Fengtianyou 663 with high-yield, high-quality and disease-resistant. It has passed variety accreditation of Guangxi. The grains of Guihui 663 and Fengtianyou 663 showed fragrance.【Conclusion】Guihui 663 is a new rice restorer line with resistant to rice blast, bacterial blight, and rice bacterial leaf streak. Moreover, It has strong restoring ability and optimize rice quality. Guihui 663 is obtained to provide excellent parent materials for quality hybrid combination which are disease-resistance, high-quality and high-yield. At the same time, it also provides important genetic base materials for rice.
【Objective】To provide outstanding parent for hybrid rice of disease-resistance, high-quality and high-yield, a strong rice restorer line with resistant to rice blast, bacterial blight, and rice bacterial leaf streak was breed.【Method】Material Hefengzhan with resistant to rice blast was receptor parent, and material Yuetaizhan with resistant to bacterial blight was donor parent. And excellent individuals were obtained with multiple disease-resistance genes by Molecular marker assisted selection technique. The individuals were selected for self intersection till they were stable. The resistance and rice quality were also detected. Agronomic traits and restoring ability were analyzed.【Result】A strong rice restorer line with resistant to rice blast genes Pi-ta,Pib and Pi54, bacterial blight and rice bacterial leaf streak genes xa5 was bred. It was named Guihui 663. Inoculations Guihui 663 with the predominant pathogenic types Ⅳ and types Ⅴof Xanthomonas oryzae pv. oryzae and representative types JZ-8 of rice bacterial leaf streak in Guangxi. The results showed resistance. Inoculations Guihui 663 with 14 rice blast, 12 of them identified resistance grade 1. The rice quality was identified as national quality grade 2 except amylose content. Guihui 663 crossed with sterile line Fengtian1 A and obtained hybrid rice combination Fengtianyou 663 with high-yield, high-quality and disease-resistant. It has passed variety accreditation of Guangxi. The grains of Guihui 663 and Fengtianyou 663 showed fragrance.【Conclusion】Guihui 663 is a new rice restorer line with resistant to rice blast, bacterial blight, and rice bacterial leaf streak. Moreover, It has strong restoring ability and optimize rice quality. Guihui 663 is obtained to provide excellent parent materials for quality hybrid combination which are disease-resistance, high-quality and high-yield. At the same time, it also provides important genetic base materials for rice.
引文
[1]阮辉辉,严成其,安德荣,等. 水稻抗白叶枯病基因的鉴定、定位和克隆进展[J]. 生物技术通讯,2008,19(3):463-467.
[2]秦钢,阎勇,马增凤,等. 水稻白叶枯病和细条病稻兼抗资源筛选及兼抗基因分析[J]. 西南农业学报,2017,30(2):354-358.
[3]董继新,董海涛,李德葆. 水稻抗瘟性研究进展[J]. 农业生物技术学报,2000,8(1):99-102.
[4]Singh G P,Srivastava M K,Singh R V. Variation and qualitative losses caused by bacterial blight in difference rice varieties[J]. Indian Phytopathology,1997,30:180-185.
[5]翟文学,朱立煌. 水稻白叶枯病抗性基因的研究与分子育种[J].生物工程进展,1999,19(6):9-15.
[6]Niňo-Liu D O,Ronald P C,Bogdanove A J. Xanthomonas oryzae,pathovars:model pathogens of a model crop[J]. Molecular Plant Pathology,2006,7(5):303-324.
[7]潘晓飚,陈凯,张强,等. 分子标记辅助选育水稻抗白叶枯病和稻瘟病多基因聚合恢复系[J]. 作物学报,2013,39(9):1582-1593.
[8]朱小源,杨健源,陈玉托,等. 引致天优998抗性丧失的稻瘟病菌小种鉴定及其致病性测定[J]. 广东农业科学,2008(12):84-86.
[9]张启军,吕川根,虞德容,等. 水稻抗病基因聚合育种研究进展[J]. 中国农学通报,2008,24(8):57-62.
[10]庄杰云,朱玉君,屠国庆,等. 多基因聚合育成优质高产杂交稻新组合中优161[J]. 杂交水稻,2010,25(5):12-14.
[11]罗世友,陈红萍,吴小燕,等. 应用分子标记辅助选育抗褐飞虱水稻恢复[J]. 分子植物育种,2015,13(11):2404-2415.
[12]张建福,曾大力,朱永生,等. 分子标记辅助选择创制抗白背飞虱水稻恢复系[J]. 中国水稻科学,2013,27(3):329-334.
[13]李进波,夏明元,戚华雄. 水稻抗稻瘟病基因Pi1和Pi2聚合系的获得及其抗性评价[J]. 安徽农业科学,2015,43(5):12-14,31.
[14]Xie X F,Chen Z W,Cao J L,et al. Toward the positional cloning of qBlsr5a,a QTL underlying resistance to bacterial leaf streak,using overlapping Sub-CSSLs in rice[J]. PLos One,2014,9(4):95751.
[15]Murray M G,Thompson W K. Rapid isolation of high molecular weight plant DNA[J]. Nucleic Acids Research,1980,8(19):4321-4325.
[16]Jia Y,Wang Z,Singh P. Development of dominant rice blast Pi-ta resistance gene markers[J]. Crop Science,2002,42(6):2145-2149.
[17]Jia Y,Wang Z,Fjellstrom R G,et al. Rice Pi-ta gene confers resistance to the major pathotypes of the rice blast fungus in the United States[J]. Phytopathology,2004,94(3):296-301.
[18]Fjellstrom R,Conawaybormans C A,Mcclung A M,et al. Development of dna markers suitable for marker assisted selection of three pi genes conferring resistance to multiple pyricularia grisea pathotypes[J]. Cropence,2004,44(5):1790-1798.
[19]Ramkumar G,Srinivasarao K,Mohan K,et al. Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54(Pikh)[J]. Molecular Breeding,2011,27 (1):129-135.
[20]陈深,钟杰,朱小源,等. 新品种绿珍8072和白香占的抗白叶枯病遗传分析及其基因检测[J]. 分子植物育种,2012,10(3):357-362.
[21]颜群,潘英华,秦学毅,等. 普通野生稻稻瘟病广谱抗性基因Pi-gx(t)的遗传分析和定位[J]. 南方农业学报,2012,43(10):1433-1437.
[22]Bonman J M,Vergael de Dios T I,Khin M M. Physiological specialization of Pyricularia oryzae in the Philippines[J]. Plant Disease,1986,70:767-769.
[23]Kauffman H E,Reddy A P K,Hsieh S P Y,et al. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae[J]. Plant Disease Reporter,1973,57(6):537-541.
[24]岑贞陆,黄思良,李容柏,等. 稻种材料抗细菌性条斑病性鉴定[J]. 安徽农业科学,2007,35(22):6850-6851.
[25]曾宇,夏秀忠,农保选,等. 广西特色香稻地方品种香味及其香味基因型的鉴定[J]. 南方农业学报,2017,48(9):1548-1553.
[26]Berner D K,Hoff B J. Inheritance of scent in American long grain rice[J]. Crop Science,1986,26(5):876-878.
[27]陈小林,颜群,李瑞芳,等. 广西稻瘟病菌生理小种鉴定及遗传多样性分析[J]. 西南农业学报,2017,30(4):767-772.
[28]阎勇,马增凤,秦钢,等. 华南常用籼稻亲本稻瘟病抗性评价及抗性基因鉴定[J]. 南方农业学报,2017,48(4):587-593.
[29]Li Z K,Sanchez A,Angeles E,Singh S,et al. Are the dominant and recessive plant disease resistance genes similar A case study of rice R genes and Xanthomonas oryzae pv. Oryzae races[J]. Genetics,2001,159:757-765.
[30]李存龙,杨芬,罗龙,等.香型软米恢复系文恢206选育及应用研究[J]. 西南农业学报,2007,20(4):581-585.
[2]秦钢,阎勇,马增凤,等. 水稻白叶枯病和细条病稻兼抗资源筛选及兼抗基因分析[J]. 西南农业学报,2017,30(2):354-358.
[3]董继新,董海涛,李德葆. 水稻抗瘟性研究进展[J]. 农业生物技术学报,2000,8(1):99-102.
[4]Singh G P,Srivastava M K,Singh R V. Variation and qualitative losses caused by bacterial blight in difference rice varieties[J]. Indian Phytopathology,1997,30:180-185.
[5]翟文学,朱立煌. 水稻白叶枯病抗性基因的研究与分子育种[J].生物工程进展,1999,19(6):9-15.
[6]Niňo-Liu D O,Ronald P C,Bogdanove A J. Xanthomonas oryzae,pathovars:model pathogens of a model crop[J]. Molecular Plant Pathology,2006,7(5):303-324.
[7]潘晓飚,陈凯,张强,等. 分子标记辅助选育水稻抗白叶枯病和稻瘟病多基因聚合恢复系[J]. 作物学报,2013,39(9):1582-1593.
[8]朱小源,杨健源,陈玉托,等. 引致天优998抗性丧失的稻瘟病菌小种鉴定及其致病性测定[J]. 广东农业科学,2008(12):84-86.
[9]张启军,吕川根,虞德容,等. 水稻抗病基因聚合育种研究进展[J]. 中国农学通报,2008,24(8):57-62.
[10]庄杰云,朱玉君,屠国庆,等. 多基因聚合育成优质高产杂交稻新组合中优161[J]. 杂交水稻,2010,25(5):12-14.
[11]罗世友,陈红萍,吴小燕,等. 应用分子标记辅助选育抗褐飞虱水稻恢复[J]. 分子植物育种,2015,13(11):2404-2415.
[12]张建福,曾大力,朱永生,等. 分子标记辅助选择创制抗白背飞虱水稻恢复系[J]. 中国水稻科学,2013,27(3):329-334.
[13]李进波,夏明元,戚华雄. 水稻抗稻瘟病基因Pi1和Pi2聚合系的获得及其抗性评价[J]. 安徽农业科学,2015,43(5):12-14,31.
[14]Xie X F,Chen Z W,Cao J L,et al. Toward the positional cloning of qBlsr5a,a QTL underlying resistance to bacterial leaf streak,using overlapping Sub-CSSLs in rice[J]. PLos One,2014,9(4):95751.
[15]Murray M G,Thompson W K. Rapid isolation of high molecular weight plant DNA[J]. Nucleic Acids Research,1980,8(19):4321-4325.
[16]Jia Y,Wang Z,Singh P. Development of dominant rice blast Pi-ta resistance gene markers[J]. Crop Science,2002,42(6):2145-2149.
[17]Jia Y,Wang Z,Fjellstrom R G,et al. Rice Pi-ta gene confers resistance to the major pathotypes of the rice blast fungus in the United States[J]. Phytopathology,2004,94(3):296-301.
[18]Fjellstrom R,Conawaybormans C A,Mcclung A M,et al. Development of dna markers suitable for marker assisted selection of three pi genes conferring resistance to multiple pyricularia grisea pathotypes[J]. Cropence,2004,44(5):1790-1798.
[19]Ramkumar G,Srinivasarao K,Mohan K,et al. Development and validation of functional marker targeting an InDel in the major rice blast disease resistance gene Pi54(Pikh)[J]. Molecular Breeding,2011,27 (1):129-135.
[20]陈深,钟杰,朱小源,等. 新品种绿珍8072和白香占的抗白叶枯病遗传分析及其基因检测[J]. 分子植物育种,2012,10(3):357-362.
[21]颜群,潘英华,秦学毅,等. 普通野生稻稻瘟病广谱抗性基因Pi-gx(t)的遗传分析和定位[J]. 南方农业学报,2012,43(10):1433-1437.
[22]Bonman J M,Vergael de Dios T I,Khin M M. Physiological specialization of Pyricularia oryzae in the Philippines[J]. Plant Disease,1986,70:767-769.
[23]Kauffman H E,Reddy A P K,Hsieh S P Y,et al. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae[J]. Plant Disease Reporter,1973,57(6):537-541.
[24]岑贞陆,黄思良,李容柏,等. 稻种材料抗细菌性条斑病性鉴定[J]. 安徽农业科学,2007,35(22):6850-6851.
[25]曾宇,夏秀忠,农保选,等. 广西特色香稻地方品种香味及其香味基因型的鉴定[J]. 南方农业学报,2017,48(9):1548-1553.
[26]Berner D K,Hoff B J. Inheritance of scent in American long grain rice[J]. Crop Science,1986,26(5):876-878.
[27]陈小林,颜群,李瑞芳,等. 广西稻瘟病菌生理小种鉴定及遗传多样性分析[J]. 西南农业学报,2017,30(4):767-772.
[28]阎勇,马增凤,秦钢,等. 华南常用籼稻亲本稻瘟病抗性评价及抗性基因鉴定[J]. 南方农业学报,2017,48(4):587-593.
[29]Li Z K,Sanchez A,Angeles E,Singh S,et al. Are the dominant and recessive plant disease resistance genes similar A case study of rice R genes and Xanthomonas oryzae pv. Oryzae races[J]. Genetics,2001,159:757-765.
[30]李存龙,杨芬,罗龙,等.香型软米恢复系文恢206选育及应用研究[J]. 西南农业学报,2007,20(4):581-585.