乙二胺四乙酸(EDTA)生物处理研究进展
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摘要
乙二胺四乙酸(EDTA)作为一种重要螯合剂,可与绝大多数金属离子配位形成1∶1的具有超强稳定结构的金属螯合物,被广泛应用于造纸、医药、食品、纺织印染等行业.大量EDTA的应用导致其在环境中的逐渐积累,这将对生态环境造成潜在危害.自1998年EDTA被首次报道可生物降解以来,国内外已针对EDTA的生物处理开展了广泛研究.本文总结归纳了目前已报道的EDTA降解菌株及其降解性能,指出EDTA的降解效果与微生物类型、EDTA金属螯合物的稳定性密切相关;概述了涉及EDTA降解的微生物菌群、降解基因与降解途径;针对EDTA高度稳定、可生化性差的问题,重点阐述了国内外关于EDTA降解优化调控的方法策略,主要包括外源物质添加、生物强化、工艺调控等;未来可从EDTA降解菌株的筛选分离、EDTA降解菌群的快速增殖以及EDTA废水处理系统中的微生物种群结构及其相互作用机制等方面开展相关研究工作.
Ehylenediaminetetraacetic acid( EDTA),as an important chelating agent,can coordinate with most metal ions to form 1: 1 EDTA-metal chelates with super stable structure. It is widely used in papermaking,medicine,food,textile printing and dyeing industries. The extensive application of EDTA leads to the gradual accumulation of EDTA in different environments,which will cause potential harm to the ecological environment. Since EDTA was first reported biodegradable in 1998,extensive research has been carried out on the biological treatment of EDTA worldwide. In this paper,the EDTA degradation strains and their degradation performance are summarized.The degradation performance of EDTA is found to be closely related to microbe species and the stability of EDTA-metal chelates. Then,the microbial communities,genes and pathways involved in EDTA degradation are overviewed. To overcome the problem of high stability and poor biodegradability for EDTA removal,the regulation strategies for EDTA biodegradation,including exogenous substances addition,bioaugmentation,process control,etc.were mainly discussed. In future,relevant research work can be carried out on the screening and isolation of EDTA-degrading bacteria,the rapid proliferation of EDTA-degrading bacteria and the microbial community structure and their interaction mechanisms in EDTA wastewater treatment systems.
Ehylenediaminetetraacetic acid( EDTA),as an important chelating agent,can coordinate with most metal ions to form 1: 1 EDTA-metal chelates with super stable structure. It is widely used in papermaking,medicine,food,textile printing and dyeing industries. The extensive application of EDTA leads to the gradual accumulation of EDTA in different environments,which will cause potential harm to the ecological environment. Since EDTA was first reported biodegradable in 1998,extensive research has been carried out on the biological treatment of EDTA worldwide. In this paper,the EDTA degradation strains and their degradation performance are summarized.The degradation performance of EDTA is found to be closely related to microbe species and the stability of EDTA-metal chelates. Then,the microbial communities,genes and pathways involved in EDTA degradation are overviewed. To overcome the problem of high stability and poor biodegradability for EDTA removal,the regulation strategies for EDTA biodegradation,including exogenous substances addition,bioaugmentation,process control,etc.were mainly discussed. In future,relevant research work can be carried out on the screening and isolation of EDTA-degrading bacteria,the rapid proliferation of EDTA-degrading bacteria and the microbial community structure and their interaction mechanisms in EDTA wastewater treatment systems.
引文
[1]林琳,袁志文.生物降解乙二胺四乙酸(EDTA)研究进展[J].净水技术,2008,27(5):10-13.
[2]Takahashi R,Suzuki M,Endo T,et al.Biodegradabilities of ethylenediamine-N,N'-disuccinic acid(EDDS)and other chelating agents[J].Bioscience Biotechnology&Biochemistry,1997,61(11):1957-1959.
[3]程艳,高静,徐红纳,等.螯合剂EDTA简介[J].化学教育,2009,30(5):4-6.
[4]Zhang S H,Zhao S M,Zhao Z M,et al.Genotyping of urinary samples stored with EDTA for forensic applications[J].Genetics&Molecular Research Gmr,2012,11(3):3007-3012.
[5]Hur D H,Lee E H,Choi M S,et al.High temperature application of EDTA solvents for iron oxide removal[J].Journal of Nuclear Materials,2001,299(3):271-273.
[6]Bloem E,Haneklaus S,Haensch R,et al.EDTA application on agricultural soils affects microelement uptake of plants[J].Science of the Total Environment,2017,577(15):166-173.
[7]Sampanpanish P,Pinpa K.Cadmium Removal from Contaminated Sediment Using EDTA and DTPA with Water Hyacinth[J].International Journal of Environmental Research,2018,12(2):1-9.
[8]Gervasio A L,Christine G,Robin B,et al.Effect of disodium EDTA chelation regimen on cardiovascular events in patients with previous myocardial infarction:the TACT randomized trial[J].JAMA:the Journal of the American Medical Association,2013,309(12):1241-1250.
[9]Shuo L I,Li L I,Cao J.Determination of ethylenediaminetetraacetic acid disodium in flour by high performance liquid chromatography[J].J Food Saf Food Qual,2017,8(2):639-642.
[10]Kaparullina E,Doronina N,Trotsenko Y.Aerobic degradation of ethylenediaminetetraacetate(review)[J].Applied Biochemistry&Microbiology,2011,47(5):460-473.
[11]Kaparullina E N,Doronina N V,Ezhov V A,et al.EDTA degradation by cells of Chelativorans oligotrophicus immobilized on a biofilter[J].Applied Biochemistry&Microbiology,2012,48(4):396-400.
[12]Oviedo C,Rodrígues J.EDTA:The chelating agent under environmental scrutiny[J].Quim Nova,2003,26(6):901-905.
[13]Marchyulenene D P,Moteyunene E B,Shuliyene R Y,et al.Effect of the EDTA complexing agent on algae[Microcystis sp.,Dunaliella viridis,Phaeodactylum tricomatum][J].Hydrobiol J,1982,18(5):73-80.
[14]Klüner T,Hempel D C,N9rtemann B.Metabolism of EDTA and its metal chelates by whole cells and cell-free extracts of strain BNC1[J].Appl Microbiol Biot,1998,49(2):194-201.
[15]Zhu X H,Chen M X,He X,et al.Bioaugmentation treatment of PV wafer manufacturing wastewater by microbial culture[J].Water Science&Technology,2015,72(5):754-761.
[16]兰书焕,陈杨武,贺玉龙,等.光伏产业清洗剂废水低温生物处理[J].应用与环境生物学报,2016,22(3):388-392.
[17]刘剑,凤依,谭雄文,等.酸改性活性炭对EDTA废水的吸附[J].材料导报,2015,29(20):81-86.
[18]王罗春,周俊,朱玲燕.微波辅助-活性炭法处理电厂EDTA锅炉清洗废水可行性研究[J].环境工程学报,2011,5(2):347-351.
[19]Pirkanniemi K,Metsrinne S,SillanpM.Degradation of EDTA and novel complexing agents in pulp and paper mill process and waste waters by Fenton's reagent[J].Journal of Hazardous Materials,2007,147(1):556-561.
[20]Liu P,Li C L,Kong X H,et al.Photocatalytic degradation of EDTA with UV/Cu(II)/H2O2process[J].Desalin Water Treat,2013,51(40):7555-7561.
[21]邵天元,李新宝,张宝锋,等.电芬顿法处理重金属络合物Ni-EDTA的研究[J].环境科学学报,2015,35(3):745-749.
[22]潘汉平,林亲铁,邱川展,等.微波-Fenton法处理EDTA-Cu-Ni废水的工况与效能[J].环境工程学报,2014,8(7):2906-2912.
[23]Liu X,Fan J H,Hao Y,et al.The degradation of EDTA by the bimetallic Fe-Cu/O2system[J].Chemical Engineering Journal,2014,250(9):354-365.
[24]杨桂蓉,魏连雨,李静,等.Co-Bi VO4薄膜电极光电处理Pb/Cu-EDTA研究[J].环境科学学报,2014,34(4):914-919.
[25]鞠峰,胡勇有,程建华,等.铁屑内电解法处理EDTA溶液中络合铜离子[J].环境科学学报,2011,31(5):897-904.
[26]Alexander M.Nonbiodegradable and other racalcitrant molecules[J].Biotechnology and Bioengineering,1973,15(4):611-647.
[27]Kaluza U,Klingelh9fer P,Taeger K.Microbial degradation of EDTA in an industrial wastewater treatment plant[J].Water Research,1998,32(9):2843-2845.
[28]Paraneeiswaran A,Shukla S K,Prashanth K,et al.Microbial reduction of[Co(III)-EDTA]-by Bacillus licheniformis SPB-2 strain isolated from a solar salt pan[J].Journal of Hazardous Materials,2015,283:582-590.
[29]Paraneeiswaran A,Shukla S K,Rao T S,et al.Removal of toxic Co-EDTA complex by a halophilic solar-salt-pan isolate Pseudomonas aeruginosa SPB-1[J].Chemosphere,2014,95(1):503-510.
[30]刘君.以EDTA为碳源条件下铜绿假单胞菌对络合状态下EDTA的降解及Cr(Ⅵ)去除效果研究[D].长沙:湖南大学;2011.
[31]Suzuki Y,Koyama N.Uptake and degradation of EDTA by Escherichia coli[J].Biodegradation,2009,20(1):39-44.
[32]Imada C,Harada Y,Kobayashi T,et al.Degradation of Ferric Chelate of Ethylenediaminetetraacetic Acid by Bacterium Isolated from Deep-Sea Stalked Barnacle[J].Marine Biotechnology,2005,7(1):21-25.
[33]Chistyakova T I,Dedyukhina E G,Satroutdinov A D,et al.EDTA-dependent bacterial strain[J].Process Biochemistry,2005,40(2):601-605.
[34]Fang H Y,Chen S C,Chen S L.Degradation of ferric EDTA by Burkhol cepacia[J].Applied Biochemistry&Biotechnology,2003,111(2):81-91.
[35]Chistyakova T I,Belikova V L,Satroutdinov A D,et al.A novel EDTA-degrading Pseudomonas sp[J].World Journal of Microbiology&Biotechnology,2003,19(9):977-980.
[36]Aidar D S,Emiliya G D,Tat’yana I C.Degradation of metal-EDTA complexes by resting cells of the bacterial strain DSM9103[J].Environ Sci Technol,2000,34(9):1715-1720.
[37]Satrutdinov A D,Dediukhina E G,Chistiakova T I,et al.[Bacterial degradation of EDTA][J].Mikrobiologiia,2003,72(1):14-18.
[38]N9rtemann B.Total degradation of EDTA by mixed cultures and a bacterial isolate[J].Appl Environ Microbiol,1992,58(2):671-676.
[39]Lauff J J,Steele D B,Coogan L A,et al.Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp[J].Applied&Environmental Microbiology,1990,56(11):3346-3353.
[40]Witschel M,Nagel S,Egli T.Identification and characterization of the two-enzyme system catalyzing oxidation of EDTA in the EDTA-degrading bacterial strain DSM 9103[J].Journal of Bacteriology,1997,179(22):6937-6943.
[41]Bohuslavek J,Payne J W,Liu Y,et al.Cloning,sequencing,and characterization of a gene cluster involved in EDTA degradation from the bacterium BNC1[J].Applied&Environmental Microbiology,2001,67(2):688-695.
[42]Yuan Z W,Vanbriesen J M.Bacterial growth yields on EDTA,NTA,and their biodegradation intermediates[J].Biodegradation,2008,19(1):41-52.
[43]Liu Y,Louie T M,Payne J,et al.Identification,purification,and characterization of iminodiacetate oxidase from the EDTA-degrading bacterium BNC1[J].Applied&Environmental Microbiology,2001,67(2):696-701.
[44]Thomas R A,Lawlor K,Bailey M,et al.Biodegradation of metal-EDTA complexes by an enriched microbial population[J].Applied&Environmental Microbiology,1998,64(4):1319-1322.
[45]Kharusi S A,Abed R M M,Dobretsov S.EDTA addition enhances bacterial respiration activities and hydrocarbon degradation in bioaugmented and non-bioaugmented oil-contaminated desert soils[J].Chemosphere,2016,147:279-286.
[46]Gao W,Gentry T J,Mehlhorn T L,et al.Characterization of Co(III)EDTA-reducing bacteria in metal-and radionuclidecontaminated groundwater[J].Geomicrobiology Journal,2010,27(1):93-100.
[47]Vanginkel C G,Stroo C A,Vandenbroucke K L.Biological removal of EDTA in conventional activated-sludge plants operated under alkaline conditions[J].Bioresouce Technol,1997,59(3):151-155.
[48]王瑞敏,黄少斌.生物法降解EDTA废水及其脱氮特性的研究[J].工业用水与废水,2009,40(6):31-34.
[49]杨晓奕,蒋展鹏,师绍琪,等.乙二胺四乙酸(EDTA)生物降解特性[J].环境科学,2001,22(2):41-45.
[50]Chen S C,Li K H,Fang H Y.Growth kinetics of EDTA biodegradation by Burkholderia cepacia[J].World Journal of Microbiology&Biotechnology,2005,21(1):11-16.
[51]Henneken L,N9rtemann B,Hempel D C.Influence of physiological conditions on EDTA degradation[J].Applied Microbiology&Biotechnology,1995,44(1-2):190-197.
[52]Kuvichkina T N,Doronina N V,Trotsenko Y A,et al.Degradation of the EDTA and EDTA complexes with metals by immobilized cells of Chelativorans oligotrophicus LPM-4 bacteria[J].Prikl Biokhim Mikrobiol,2012,48(6):564-568.
[53]Ambily P S,Jisha M S.Metabolic profile of sodium dodecyl sulphate(SDS)biodegradation by Pseudomonas aeruginosa(MTCC10311)[J].Journal of Environmental Biology,2014,35(5):827-831.
[54]Chen Y,Lan S,Wang L,et al.A review:Driving factors and regulation strategies of microbial community structure and dynamics in wastewater treatment systems[J].Chemosphere,2017,174:173-182.
[55]陈杨武.不同胁迫下清洗剂降解菌群代谢活性与群落结构响应及调控[D].北京:中国科学院大学,2018.
[56]Henneken L,N9rtemann B,Hempel D C.Biological degradation of EDTA:Reaction kinetics and technical approach[J].Journal of Chemical Technology&Biotechnology Biotechnology,2010,73(2):144-152.
[2]Takahashi R,Suzuki M,Endo T,et al.Biodegradabilities of ethylenediamine-N,N'-disuccinic acid(EDDS)and other chelating agents[J].Bioscience Biotechnology&Biochemistry,1997,61(11):1957-1959.
[3]程艳,高静,徐红纳,等.螯合剂EDTA简介[J].化学教育,2009,30(5):4-6.
[4]Zhang S H,Zhao S M,Zhao Z M,et al.Genotyping of urinary samples stored with EDTA for forensic applications[J].Genetics&Molecular Research Gmr,2012,11(3):3007-3012.
[5]Hur D H,Lee E H,Choi M S,et al.High temperature application of EDTA solvents for iron oxide removal[J].Journal of Nuclear Materials,2001,299(3):271-273.
[6]Bloem E,Haneklaus S,Haensch R,et al.EDTA application on agricultural soils affects microelement uptake of plants[J].Science of the Total Environment,2017,577(15):166-173.
[7]Sampanpanish P,Pinpa K.Cadmium Removal from Contaminated Sediment Using EDTA and DTPA with Water Hyacinth[J].International Journal of Environmental Research,2018,12(2):1-9.
[8]Gervasio A L,Christine G,Robin B,et al.Effect of disodium EDTA chelation regimen on cardiovascular events in patients with previous myocardial infarction:the TACT randomized trial[J].JAMA:the Journal of the American Medical Association,2013,309(12):1241-1250.
[9]Shuo L I,Li L I,Cao J.Determination of ethylenediaminetetraacetic acid disodium in flour by high performance liquid chromatography[J].J Food Saf Food Qual,2017,8(2):639-642.
[10]Kaparullina E,Doronina N,Trotsenko Y.Aerobic degradation of ethylenediaminetetraacetate(review)[J].Applied Biochemistry&Microbiology,2011,47(5):460-473.
[11]Kaparullina E N,Doronina N V,Ezhov V A,et al.EDTA degradation by cells of Chelativorans oligotrophicus immobilized on a biofilter[J].Applied Biochemistry&Microbiology,2012,48(4):396-400.
[12]Oviedo C,Rodrígues J.EDTA:The chelating agent under environmental scrutiny[J].Quim Nova,2003,26(6):901-905.
[13]Marchyulenene D P,Moteyunene E B,Shuliyene R Y,et al.Effect of the EDTA complexing agent on algae[Microcystis sp.,Dunaliella viridis,Phaeodactylum tricomatum][J].Hydrobiol J,1982,18(5):73-80.
[14]Klüner T,Hempel D C,N9rtemann B.Metabolism of EDTA and its metal chelates by whole cells and cell-free extracts of strain BNC1[J].Appl Microbiol Biot,1998,49(2):194-201.
[15]Zhu X H,Chen M X,He X,et al.Bioaugmentation treatment of PV wafer manufacturing wastewater by microbial culture[J].Water Science&Technology,2015,72(5):754-761.
[16]兰书焕,陈杨武,贺玉龙,等.光伏产业清洗剂废水低温生物处理[J].应用与环境生物学报,2016,22(3):388-392.
[17]刘剑,凤依,谭雄文,等.酸改性活性炭对EDTA废水的吸附[J].材料导报,2015,29(20):81-86.
[18]王罗春,周俊,朱玲燕.微波辅助-活性炭法处理电厂EDTA锅炉清洗废水可行性研究[J].环境工程学报,2011,5(2):347-351.
[19]Pirkanniemi K,Metsrinne S,SillanpM.Degradation of EDTA and novel complexing agents in pulp and paper mill process and waste waters by Fenton's reagent[J].Journal of Hazardous Materials,2007,147(1):556-561.
[20]Liu P,Li C L,Kong X H,et al.Photocatalytic degradation of EDTA with UV/Cu(II)/H2O2process[J].Desalin Water Treat,2013,51(40):7555-7561.
[21]邵天元,李新宝,张宝锋,等.电芬顿法处理重金属络合物Ni-EDTA的研究[J].环境科学学报,2015,35(3):745-749.
[22]潘汉平,林亲铁,邱川展,等.微波-Fenton法处理EDTA-Cu-Ni废水的工况与效能[J].环境工程学报,2014,8(7):2906-2912.
[23]Liu X,Fan J H,Hao Y,et al.The degradation of EDTA by the bimetallic Fe-Cu/O2system[J].Chemical Engineering Journal,2014,250(9):354-365.
[24]杨桂蓉,魏连雨,李静,等.Co-Bi VO4薄膜电极光电处理Pb/Cu-EDTA研究[J].环境科学学报,2014,34(4):914-919.
[25]鞠峰,胡勇有,程建华,等.铁屑内电解法处理EDTA溶液中络合铜离子[J].环境科学学报,2011,31(5):897-904.
[26]Alexander M.Nonbiodegradable and other racalcitrant molecules[J].Biotechnology and Bioengineering,1973,15(4):611-647.
[27]Kaluza U,Klingelh9fer P,Taeger K.Microbial degradation of EDTA in an industrial wastewater treatment plant[J].Water Research,1998,32(9):2843-2845.
[28]Paraneeiswaran A,Shukla S K,Prashanth K,et al.Microbial reduction of[Co(III)-EDTA]-by Bacillus licheniformis SPB-2 strain isolated from a solar salt pan[J].Journal of Hazardous Materials,2015,283:582-590.
[29]Paraneeiswaran A,Shukla S K,Rao T S,et al.Removal of toxic Co-EDTA complex by a halophilic solar-salt-pan isolate Pseudomonas aeruginosa SPB-1[J].Chemosphere,2014,95(1):503-510.
[30]刘君.以EDTA为碳源条件下铜绿假单胞菌对络合状态下EDTA的降解及Cr(Ⅵ)去除效果研究[D].长沙:湖南大学;2011.
[31]Suzuki Y,Koyama N.Uptake and degradation of EDTA by Escherichia coli[J].Biodegradation,2009,20(1):39-44.
[32]Imada C,Harada Y,Kobayashi T,et al.Degradation of Ferric Chelate of Ethylenediaminetetraacetic Acid by Bacterium Isolated from Deep-Sea Stalked Barnacle[J].Marine Biotechnology,2005,7(1):21-25.
[33]Chistyakova T I,Dedyukhina E G,Satroutdinov A D,et al.EDTA-dependent bacterial strain[J].Process Biochemistry,2005,40(2):601-605.
[34]Fang H Y,Chen S C,Chen S L.Degradation of ferric EDTA by Burkhol cepacia[J].Applied Biochemistry&Biotechnology,2003,111(2):81-91.
[35]Chistyakova T I,Belikova V L,Satroutdinov A D,et al.A novel EDTA-degrading Pseudomonas sp[J].World Journal of Microbiology&Biotechnology,2003,19(9):977-980.
[36]Aidar D S,Emiliya G D,Tat’yana I C.Degradation of metal-EDTA complexes by resting cells of the bacterial strain DSM9103[J].Environ Sci Technol,2000,34(9):1715-1720.
[37]Satrutdinov A D,Dediukhina E G,Chistiakova T I,et al.[Bacterial degradation of EDTA][J].Mikrobiologiia,2003,72(1):14-18.
[38]N9rtemann B.Total degradation of EDTA by mixed cultures and a bacterial isolate[J].Appl Environ Microbiol,1992,58(2):671-676.
[39]Lauff J J,Steele D B,Coogan L A,et al.Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp[J].Applied&Environmental Microbiology,1990,56(11):3346-3353.
[40]Witschel M,Nagel S,Egli T.Identification and characterization of the two-enzyme system catalyzing oxidation of EDTA in the EDTA-degrading bacterial strain DSM 9103[J].Journal of Bacteriology,1997,179(22):6937-6943.
[41]Bohuslavek J,Payne J W,Liu Y,et al.Cloning,sequencing,and characterization of a gene cluster involved in EDTA degradation from the bacterium BNC1[J].Applied&Environmental Microbiology,2001,67(2):688-695.
[42]Yuan Z W,Vanbriesen J M.Bacterial growth yields on EDTA,NTA,and their biodegradation intermediates[J].Biodegradation,2008,19(1):41-52.
[43]Liu Y,Louie T M,Payne J,et al.Identification,purification,and characterization of iminodiacetate oxidase from the EDTA-degrading bacterium BNC1[J].Applied&Environmental Microbiology,2001,67(2):696-701.
[44]Thomas R A,Lawlor K,Bailey M,et al.Biodegradation of metal-EDTA complexes by an enriched microbial population[J].Applied&Environmental Microbiology,1998,64(4):1319-1322.
[45]Kharusi S A,Abed R M M,Dobretsov S.EDTA addition enhances bacterial respiration activities and hydrocarbon degradation in bioaugmented and non-bioaugmented oil-contaminated desert soils[J].Chemosphere,2016,147:279-286.
[46]Gao W,Gentry T J,Mehlhorn T L,et al.Characterization of Co(III)EDTA-reducing bacteria in metal-and radionuclidecontaminated groundwater[J].Geomicrobiology Journal,2010,27(1):93-100.
[47]Vanginkel C G,Stroo C A,Vandenbroucke K L.Biological removal of EDTA in conventional activated-sludge plants operated under alkaline conditions[J].Bioresouce Technol,1997,59(3):151-155.
[48]王瑞敏,黄少斌.生物法降解EDTA废水及其脱氮特性的研究[J].工业用水与废水,2009,40(6):31-34.
[49]杨晓奕,蒋展鹏,师绍琪,等.乙二胺四乙酸(EDTA)生物降解特性[J].环境科学,2001,22(2):41-45.
[50]Chen S C,Li K H,Fang H Y.Growth kinetics of EDTA biodegradation by Burkholderia cepacia[J].World Journal of Microbiology&Biotechnology,2005,21(1):11-16.
[51]Henneken L,N9rtemann B,Hempel D C.Influence of physiological conditions on EDTA degradation[J].Applied Microbiology&Biotechnology,1995,44(1-2):190-197.
[52]Kuvichkina T N,Doronina N V,Trotsenko Y A,et al.Degradation of the EDTA and EDTA complexes with metals by immobilized cells of Chelativorans oligotrophicus LPM-4 bacteria[J].Prikl Biokhim Mikrobiol,2012,48(6):564-568.
[53]Ambily P S,Jisha M S.Metabolic profile of sodium dodecyl sulphate(SDS)biodegradation by Pseudomonas aeruginosa(MTCC10311)[J].Journal of Environmental Biology,2014,35(5):827-831.
[54]Chen Y,Lan S,Wang L,et al.A review:Driving factors and regulation strategies of microbial community structure and dynamics in wastewater treatment systems[J].Chemosphere,2017,174:173-182.
[55]陈杨武.不同胁迫下清洗剂降解菌群代谢活性与群落结构响应及调控[D].北京:中国科学院大学,2018.
[56]Henneken L,N9rtemann B,Hempel D C.Biological degradation of EDTA:Reaction kinetics and technical approach[J].Journal of Chemical Technology&Biotechnology Biotechnology,2010,73(2):144-152.
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