运动对2型糖尿病小鼠内皮祖细胞功能的影响
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摘要
目的探讨不同运动方式对2型糖尿病小鼠内皮祖细胞(EPCs)功能的影响,明确2型糖尿病小鼠预防心血管并发症的最佳运动方式。方法 8周龄雄性2型糖尿病小鼠随机分为4组:对照组、有氧运动组、抗阻运动组、联合运动组。对照组不作运动干预;有氧运动组进行无负重跑步运动;抗阻运动组进行尾部负重爬梯运动;联合运动组隔日交替进行有氧运动和抗阻运动。运动干预1、2、4周时处死小鼠,体外分离培养骨髓源性EPCs并检测其增殖、粘附、迁移及血管生成能力。结果组间比较:运动干预4周时,各运动组EPCs粘附、迁移及血管生成能力均强于对照组,以联合运动效果最优。组内比较:各运动组EPCs粘附、迁移和血管生成能力在运动干预第2周时强于第1周时,增殖能力在第4周时强于第1周时(均P<0.05)。结论与有氧运动和抗阻运动相比,联合运动在预防2型糖尿病小鼠心血管并发症方面效果更佳,运动效果随着时间的延长而增强。
Objective To explore the effects of different exercise modes on functions of endothelial progenitor cells(EPCs)and to find out the best way to prevent cardiovascular complications in mice with type 2 diabetes.Methods Male mice with type 2 diabetes aged 8 weeks were randomly divided into control group,aerobic training(AT)group,resistant training(RT)group and AT+RT group.Mice in the control group were kept sedentary.Mice in AT group performed treadmill running.Burdened with a certain weight,mice in the RT group climbed the man-made ladder.Mice in AT+RT group performed aerobic training and resistant training in turn.Mice were killed at the 1 st,2 nd,4 th week of intervention.Bone marrow-derived EPCs were isolated and its functions of proliferation,adhesion,migration and angiogenesis were identified.Results Compared with the control group,the EPCs in AT,RT and AT+RT showed better abilities to adhere,migrate and generate new vessel at the end of 4 th week,among which AT+RT showed the best ability.Improvement in the adhesion,migration and angiogenesis of the EPCs were observed at the end of 2 rd than that at 1 st week(P<0.05)and improvement in proliferative ability was observed at the end of the 4 th week then that at 1 st week(P<0.05).Conclusion Compared to aerobic training or resistant training,combined aerobic and resistant training is more effective for preventing cardiovascular complications in mice with type 2 diabetes and the positive effects enhanced with the extension of exercise time.
Objective To explore the effects of different exercise modes on functions of endothelial progenitor cells(EPCs)and to find out the best way to prevent cardiovascular complications in mice with type 2 diabetes.Methods Male mice with type 2 diabetes aged 8 weeks were randomly divided into control group,aerobic training(AT)group,resistant training(RT)group and AT+RT group.Mice in the control group were kept sedentary.Mice in AT group performed treadmill running.Burdened with a certain weight,mice in the RT group climbed the man-made ladder.Mice in AT+RT group performed aerobic training and resistant training in turn.Mice were killed at the 1 st,2 nd,4 th week of intervention.Bone marrow-derived EPCs were isolated and its functions of proliferation,adhesion,migration and angiogenesis were identified.Results Compared with the control group,the EPCs in AT,RT and AT+RT showed better abilities to adhere,migrate and generate new vessel at the end of 4 th week,among which AT+RT showed the best ability.Improvement in the adhesion,migration and angiogenesis of the EPCs were observed at the end of 2 rd than that at 1 st week(P<0.05)and improvement in proliferative ability was observed at the end of the 4 th week then that at 1 st week(P<0.05).Conclusion Compared to aerobic training or resistant training,combined aerobic and resistant training is more effective for preventing cardiovascular complications in mice with type 2 diabetes and the positive effects enhanced with the extension of exercise time.
引文
[1]Selvin E,Lazo M,Chen Y,et al.Diabetes mellitus,prediabetes,and incidence of subclinical myocardial damage[J].Circulation,2014,130(16):1374-1382.
[2]Hill JM,Zalos G,Halcox JP,et al.Circulating endothelial progenitor cells,vascular function,and cardiovascular risk[J].The New England Journal of Medicine,2003,348(7):593-600.
[3]Rigato M,Bittante C,Albiero M,et al.Circulating progenitor cell count predicts microvascular outcomes in type 2 diabetic patients[J].Journal of Clinical Endocrinology&Metabolism,2015,100(7):2666-2672.
[4]曾会群,韩淑英.运动疗法对社区糖尿病患者血液生化指标的影响[J].现代预防医学,2011,38(4):643-644.
[5]刘君雯,何奇芳.有氧运动对大鼠高脂血症及动脉粥样硬化相关生化指标的影响[J].现代预防医学,2014,41(23):4354-4356.
[6]Tsai HH,Lin CP,Lin YH,et al.High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cells and depressed shedding of vascular endothelial cells undergoing hypoxia[J].European Journal of Applied Physiology,2016,116(11/12):2375-2388.
[7]Sandri M,Viehmann M,Adams V,et al.Chronic heart failure and aging-effects of exercise training on endothelial function and mechanisms of endothelial regeneration:Results from the Leipzig Exercise Intervention in Chronic heart failure and Aging(LEICA)study[J].European Journal of Preventive Cardiology,2016,23(4):349-358.
[8]龚晓明,郑澜,瞿树林.有氧运动对中老年大鼠外周血内皮祖细胞增殖及黏附能力的影响[J].现代预防医学,2014,41(3):527-530,537.
[9]Lin YY,Lee SD,Su CT,et al.Long-term treadmill training ameliorates endothelium-dependent vasorelaxation mediated by insulin and insulin-like growth factor-1 in hypertension[J].Journal of Applied Physiology,2015,119(6):663-669.
[10]Lee S,Farrar RP.Resistance training induces muscle-specific changes in muscle mass and function in rat[J].Journal of Exercise Physiology Online,2003,6(2):80-87.
[11]Mann S,Beedie C,Balducci S,et al.Changes in insulin sensitivity in response to different modalities of exercise:a review of the evidence[J].Diabetes-Metabolism:Research and Reviews,2014,30(4):257-268.
[12]Ulrich L,Nikos W,Andreas L,et al.Physical training increases endothelial progenitor cells,inhibits neointima formation,and enhances angiogenesis[J].Circulation,2004,109(2):220-226.
[13]Park JH,Miyashita M,Kwon YC,et al.A 12-week after-school physical activity programme improves endothelial cell function in overweight and obese children:a randomised controlled study[J].BMC Pediatrics,2012,12(1):111-111.
[14]Ross MD,Wekesa AL,Phelan JP,et al.Resistance exercise increases endothelial progenitor cells and angiogenic factors[J].Medicine and Science in Sports and Exercise,2014,46(1):16-23.
[15]Ribeiro F,Ribeiro IP,Goncalves AC,et al.Effects of resistance exercise on endothelial progenitor cell mobilization in women[J].Scientific Reports,2017,7(1):17880.
[16]Huang PH,Chen YH,Wang CH,et al.Matrix metalloproteinase-9is essential for Ischemia-Induced neovascularization by modulating bone Marrow-Derived endothelial progenitor cells[J].Arteriosclerosis Thrombosis and Vascular Biology,2009,29(8):1U58-1179.
[17]Chang E,Paterno J,Duscher D,et al.Exercise induces stromal Cell-Derived factor-1 alpha-Mediated release of endothelial progenitor cells with increased vasculogenic function[J].Plastic and Reconstructive Surgery,2015,135(2):340E-350E.
[18]Lutz AH,Blumenthal JB,Landers-Ramos RQ.Exercise-induced endothelial progenitor cell mobilization is attenuated in impaired glucose tolerance and type 2 diabetes[J].Journal of Applied Physiology,2016,121(1):36-41.
[2]Hill JM,Zalos G,Halcox JP,et al.Circulating endothelial progenitor cells,vascular function,and cardiovascular risk[J].The New England Journal of Medicine,2003,348(7):593-600.
[3]Rigato M,Bittante C,Albiero M,et al.Circulating progenitor cell count predicts microvascular outcomes in type 2 diabetic patients[J].Journal of Clinical Endocrinology&Metabolism,2015,100(7):2666-2672.
[4]曾会群,韩淑英.运动疗法对社区糖尿病患者血液生化指标的影响[J].现代预防医学,2011,38(4):643-644.
[5]刘君雯,何奇芳.有氧运动对大鼠高脂血症及动脉粥样硬化相关生化指标的影响[J].现代预防医学,2014,41(23):4354-4356.
[6]Tsai HH,Lin CP,Lin YH,et al.High-intensity Interval training enhances mobilization/functionality of endothelial progenitor cells and depressed shedding of vascular endothelial cells undergoing hypoxia[J].European Journal of Applied Physiology,2016,116(11/12):2375-2388.
[7]Sandri M,Viehmann M,Adams V,et al.Chronic heart failure and aging-effects of exercise training on endothelial function and mechanisms of endothelial regeneration:Results from the Leipzig Exercise Intervention in Chronic heart failure and Aging(LEICA)study[J].European Journal of Preventive Cardiology,2016,23(4):349-358.
[8]龚晓明,郑澜,瞿树林.有氧运动对中老年大鼠外周血内皮祖细胞增殖及黏附能力的影响[J].现代预防医学,2014,41(3):527-530,537.
[9]Lin YY,Lee SD,Su CT,et al.Long-term treadmill training ameliorates endothelium-dependent vasorelaxation mediated by insulin and insulin-like growth factor-1 in hypertension[J].Journal of Applied Physiology,2015,119(6):663-669.
[10]Lee S,Farrar RP.Resistance training induces muscle-specific changes in muscle mass and function in rat[J].Journal of Exercise Physiology Online,2003,6(2):80-87.
[11]Mann S,Beedie C,Balducci S,et al.Changes in insulin sensitivity in response to different modalities of exercise:a review of the evidence[J].Diabetes-Metabolism:Research and Reviews,2014,30(4):257-268.
[12]Ulrich L,Nikos W,Andreas L,et al.Physical training increases endothelial progenitor cells,inhibits neointima formation,and enhances angiogenesis[J].Circulation,2004,109(2):220-226.
[13]Park JH,Miyashita M,Kwon YC,et al.A 12-week after-school physical activity programme improves endothelial cell function in overweight and obese children:a randomised controlled study[J].BMC Pediatrics,2012,12(1):111-111.
[14]Ross MD,Wekesa AL,Phelan JP,et al.Resistance exercise increases endothelial progenitor cells and angiogenic factors[J].Medicine and Science in Sports and Exercise,2014,46(1):16-23.
[15]Ribeiro F,Ribeiro IP,Goncalves AC,et al.Effects of resistance exercise on endothelial progenitor cell mobilization in women[J].Scientific Reports,2017,7(1):17880.
[16]Huang PH,Chen YH,Wang CH,et al.Matrix metalloproteinase-9is essential for Ischemia-Induced neovascularization by modulating bone Marrow-Derived endothelial progenitor cells[J].Arteriosclerosis Thrombosis and Vascular Biology,2009,29(8):1U58-1179.
[17]Chang E,Paterno J,Duscher D,et al.Exercise induces stromal Cell-Derived factor-1 alpha-Mediated release of endothelial progenitor cells with increased vasculogenic function[J].Plastic and Reconstructive Surgery,2015,135(2):340E-350E.
[18]Lutz AH,Blumenthal JB,Landers-Ramos RQ.Exercise-induced endothelial progenitor cell mobilization is attenuated in impaired glucose tolerance and type 2 diabetes[J].Journal of Applied Physiology,2016,121(1):36-41.