健胃合剂质量控制方法研究
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摘要
目的建立健胃合剂质量控制的方法。方法采用薄层色谱法对甘草、黄连和麸炒枳壳进行定性鉴别;采用HPLC测定柚皮苷含量。色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm),流动相为甲醇-0.2%磷酸(40∶60),检测波长为283 nm,流速为1.0 mL·min~(-1),柱温为30℃,进样量为5μL。结果建立的薄层色谱法样品斑点清晰,分离度好。含量测定结果显示,柚皮苷在0.005 33～0.085 23 mg·mL~(-1)内线性关系良好,平均回收率为99.42%,RSD为0.76%。结论该方法准确可靠,专属性好,可以用于健胃合剂的质量控制。
OBJECTIVE To establish the quality control method of Jianwei mixture. METHODS Thin layer chromatography(TLC) was used to identify Glycyrrhizae Radix et Rhizoma, Coptidis Rhizoma, stir-baked Aurantii Fructus in bran. Determination of naringin content by HPLC. The mobile phase for determining naringin was methanol-0.2% phosphoric acid(40∶60), the UV detection wavelength was 283 nm, Diamonsil C_(18)(250 mm×4.6 mm, 5#μm) column was used for the determination, and the flow rate was 1.0 mL·min~(-1), the column temperature was 30 ℃ and the injection volume was 5 μL.RESULTS TLC showed clear spots and good resolution. The linearity of naringin was in the range of 0.005 33-0.085 23 mg·mL~(-1),the average recovery of naringin was 99.42%, RSD was 0.76%. CONCLUSION The method is accurate, reliable and exclusive,it can be used for the quality control of Jianwei mixture.
OBJECTIVE To establish the quality control method of Jianwei mixture. METHODS Thin layer chromatography(TLC) was used to identify Glycyrrhizae Radix et Rhizoma, Coptidis Rhizoma, stir-baked Aurantii Fructus in bran. Determination of naringin content by HPLC. The mobile phase for determining naringin was methanol-0.2% phosphoric acid(40∶60), the UV detection wavelength was 283 nm, Diamonsil C_(18)(250 mm×4.6 mm, 5#μm) column was used for the determination, and the flow rate was 1.0 mL·min~(-1), the column temperature was 30 ℃ and the injection volume was 5 μL.RESULTS TLC showed clear spots and good resolution. The linearity of naringin was in the range of 0.005 33-0.085 23 mg·mL~(-1),the average recovery of naringin was 99.42%, RSD was 0.76%. CONCLUSION The method is accurate, reliable and exclusive,it can be used for the quality control of Jianwei mixture.
引文
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[8]王维庭,王雪梅,徐恒雁,等.清肠疏风凉血胶囊的质量标准研究[J].中国现代药物应用,2016,10(7):282-284.
[2]洛英霞.健心颗粒中丹参、黄芪、甘草、玄参的薄层色谱鉴别[J].实用中医内科杂志,2006,20(1):91.
[3]段国玲,王丽,赵春香.清咽利喉颗粒主成分的薄层色谱鉴别[J].中国药业,2011,20(24):45.
[4]王光函,赵玥,邹桂欣.化胃舒颗粒的定性鉴别研究[J].时珍国医国药,2012,23(8):1978-1979.
[5]GUO S J,DENG X M,SHEN Z Q.TLC identification of coptidis rhizama and Forsythia in qingwen baidu keli[J].Acta Ecol Animalis Domastici(家畜生态学报),2012,33(5):68-70.
[6]刘红,刘华.薄层色谱法分离黄连素、咖啡因时展开剂的选择[J].贵阳医学院学报,2005,30(4):378-381.
[7]LIU J L.Study on quality standards for tiaogu tablet[J].Anhui Med Pharm J(安徽医药),2008,12(2):125-126.
[8]王维庭,王雪梅,徐恒雁,等.清肠疏风凉血胶囊的质量标准研究[J].中国现代药物应用,2016,10(7):282-284.