2011—2015年环洞庭湖区H3亚型禽流感病毒的分离鉴定与遗传演化
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摘要
为了解环洞庭湖地区家禽H3亚型禽流感病毒的感染情况和进化规律,笔者对2011—2015年湖南省环洞庭湖地区的多个活禽市场与散养鸭场分离的31株H3亚型禽流感病毒进行基因测序和进化分析。结果表明:所有病毒的HA裂解位点不含连续的碱性氨基酸,为低致病性禽流感病毒的分子特征;各基因片段进化树显示部分病毒的PA与PB2基因与H5在相同分支,部分病毒的NP、PA、PB1与H7处于相同分支,表明H3可能与H5、H7亚型的禽流感病毒发生了复杂的基因交换;散养家禽分离的部分H3亚型禽流感病毒内部基因与越南、韩国等周边国家野鸟源的H3、H6、H7、H11等亚型同源性较高,可能有相同的进化来源。进一步将分离的病毒进行全基因比对,可划分出21种不同的病毒基因型。环洞庭湖地区H3亚型禽流感病毒基因来源复杂,表现出明显的遗传多样性。
To better understand the situation and evolution of poultry infected with H3 subtype avian influenza viruses around Dongting Lake,active monitoring at live-poultry markets and Freerange duck farms at this region were implemented in 2011-2015,and a total of 31 strains of H3 subtype AIV were isolated.Gene sequencing and evolutionary analysis of these strains were conducted in our study.Sequencing results showed that all the viruses were located in the Eurasian lineage except for two H3 N8 sin North America lineage,all isolates were low pathogenic avian influenza virus.Phylogenetic tree showed that PAand PB2 genes of some viruses were clustered with H5,while NP,PAand PB1 were clustered with H7,suggesting that complicated gene exchange were occurred.Internal genes of some viruses isolated from free-range poultry show very high homology with wild birds origin H3,H7,H11 from neighboring countries such as Vietnam and South Korea,may share the same evolutionary origin.Further genome sequence alignment can divided all viruses into 21 different genotypes.The source of H3 subtype avian influenza virus in the Dongting Lake area is complicated,showing a clear genetic diversity.
To better understand the situation and evolution of poultry infected with H3 subtype avian influenza viruses around Dongting Lake,active monitoring at live-poultry markets and Freerange duck farms at this region were implemented in 2011-2015,and a total of 31 strains of H3 subtype AIV were isolated.Gene sequencing and evolutionary analysis of these strains were conducted in our study.Sequencing results showed that all the viruses were located in the Eurasian lineage except for two H3 N8 sin North America lineage,all isolates were low pathogenic avian influenza virus.Phylogenetic tree showed that PAand PB2 genes of some viruses were clustered with H5,while NP,PAand PB1 were clustered with H7,suggesting that complicated gene exchange were occurred.Internal genes of some viruses isolated from free-range poultry show very high homology with wild birds origin H3,H7,H11 from neighboring countries such as Vietnam and South Korea,may share the same evolutionary origin.Further genome sequence alignment can divided all viruses into 21 different genotypes.The source of H3 subtype avian influenza virus in the Dongting Lake area is complicated,showing a clear genetic diversity.
引文
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[18]关立峥.中国H3N2(2009-2014年)和H5(2015-2016年)亚型禽流感病毒的生物学特性研究[D].兰州:甘肃农业大学,2017.GUAN L Z.Biological characterization of H3N2(from 2009to 2014)and H5(from 2015to 2016)avian influenza viruses in China[D].Lanzhou:Gansu Agricultural University,2017.(in Chinese)
[2]NEUMANN G,NODA T,KAWAOKA Y.Emergence and pandemic potential of swine-origin H1N1influenza virus[J].Nature,2009,459(7249):931-939.
[3]SCHOLTISSEK C,ROHDE W,VON HOYNIN-GEN V,et al.On the origin of the human influenza virus subtypes H2N2 and H3N2[J].Virology,1978,87(1):13-20.
[4]PAPPAS C,VISWANATHAN K,CHANDRASEKA-RAN A,et al.Receptor specificity and transmission of H2N2subtype viruses isolated from the pandemic of1957[J].PLoS One,2010,5(6):e11158.
[5]SONG M S,OH T K,MOON H J,et al.Ecology of H3avian influenza viruses in Korea and assessment of their pathogenic potentials[J].J Gen Virol,2008,89:949-957.
[6]ZHOU H B,ZHANG A D,CHEN H C,et al.Emergence of novel reassortant H3N2influenza viruses among ducks in China[J].Arch Virol,2011,156(6):1045-1048.
[7]SONG D,KANG B,LEE C,et al.Transmission of avian influenza virus(H3N2)to dogs[J].Emerg Infect Dis,2008,14(5):741-746.
[8]ANTHONY S J,ST LEGER J A,PUGLIARES K,et al.Emergence of fatal avian influenza in New England harbor seals[J].mBio,2012,3(4):e00166-12.
[9]LI C,YU M,LIU L T,et al.Characterization of a novel H3N2influenza virus isolated from domestic ducks in China[J].Virus Genes,2016,52(4):568-572.
[10]YANG D Q,LIU J,JU H B,et al.Genetic analysis of H3N2avian influenza viruses isolated from live poultry markets and poultry slaughterhouses in Shanghai,China in 2013[J].Virus Genes,2015,51(1):25-32.
[11]崔鹏飞,彭志,张芳,等.四株鸭源H3N8亚型禽流感病毒对SPF鸡的感染性分析[J].畜牧兽医学报,2016,47(6):1209-1214.CUI P F,PENG Z,ZHANG F,et al.Infectivity of four H3N8avian influenza viruses of duck origin in SPF chickens[J].Acta Veterinaria et Zootechnica Sinica,2016,47(6):1209-1214.(in Chinese)
[12]钟福生,王焰新,邓学建,等.洞庭湖湿地珍稀濒危鸟类群落组成及多样性[J].生态环境,2007,16(5):1485-1491.ZHONG F S,WANG Y X,DENG X J,et al.Species diversity of rare,endangered and national key protected waterfowls in Dongting Lake wetlands[J].Ecology and Environment,2007,16(5):1485-1491.(in Chinese)
[13]MUNSTER V J,BAAS C,LEXMOND P,et al.Spatial,temporal,and species variation in prevalence of influenza A viruses in wild migratory birds[J].PLoS Pathog,2007,3(5):e61.
[14]HOFFMANN E,STECH J,GUAN Y,et al.Universal primer set for the full-length amplification of all influenza A viruses[J].Arch Virol,2001,146(12):2275-2289.
[15]KANG H M,LEE E K,SONG B M,et al.Novel reassortant influenza A(H5N8)viruses among inoculated domestic and wild ducks,South Korea,2014[J].Emerg Infect Dis,2015,21(2):298-304.
[16]POHLMANN A,STARICK E,GRUND C,et al.Swarm incursions of reassortants of highly pathogenic avian influenza virus strains H5N8and H5N5,clade2.3.4.4b,Germany,winter 2016/17[J].Sci Rep,2018,8(1):15.
[17]彭志.2014~2015年环洞庭湖与鄱阳湖散养鸭群禽流感病毒感染情况调查[D].长沙:湖南农业大学,2016.PENG Z.Investigation of infection of Avian Influenza in domestic duck in Dongting Lake and Poyang Lake from 2014to 2015[D].Changsha:Hunan Agricultural University,2016.(in Chinese)
[18]关立峥.中国H3N2(2009-2014年)和H5(2015-2016年)亚型禽流感病毒的生物学特性研究[D].兰州:甘肃农业大学,2017.GUAN L Z.Biological characterization of H3N2(from 2009to 2014)and H5(from 2015to 2016)avian influenza viruses in China[D].Lanzhou:Gansu Agricultural University,2017.(in Chinese)
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