以对氨基苯酚盐酸盐为紫外吸收试剂的反相色谱-间接紫外检测法分析烷基磺酸盐
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摘要
本文以对氨基苯酚盐酸盐为背景紫外吸收试剂,采用间接紫外检测的反相色谱法分离检测辛烷磺酸盐、癸烷磺酸盐和十二烷基磺酸盐三种无紫外吸收的烷基磺酸盐。采用反相C18色谱柱分离,流动相为对氨基苯酚盐酸盐溶液-乙腈,检测波长为210nm,实现了三种目标烷基磺酸盐的分离和检测。考察了紫外吸收试剂种类、有机溶剂种类和浓度、对氨基苯酚盐酸盐浓度及其它色谱分析条件等对分离分析的影响,并探讨了影响规律。对氨基苯酚盐酸盐既是背景紫外吸收试剂,又是有效的洗脱剂组分。在选择的色谱条件下,三种目标烷基磺酸盐可在15min内完全分离和检测。辛烷磺酸盐、癸烷磺酸盐、十二烷基磺酸盐的检出限分别为0.13、0.12和0.97mg/L。将方法应用于84-消毒液的测定,加标回收率大于91%,满足日常定量分析要求。
An approach for the determination of three kinds of alkyl sulfonates(octane-1-sulfonate,decane-1-sulfonate and dodecyl-1-sulfonate)without ultraviolet absorption by reversed-phase chromatography using 4-aminophenol hydrochloride as the background ultraviolet reagent of indirect ultraviolet detection was developed in the paper.The separation and detection of three alkyl sulfonates were achieved by using C18 reversed phase column and 4-aminophenol hydrochloride/acetonitrile as mobile phases.The effects of ultraviolet absorption reagents,organic solvents,4-aminophenol hydrochloride concentration and the other chromatographic conditions on the separation and detection of alkyl sulfonates were investigated.The retention rules of alkyl sulfonates were discussed and the chromatographic conditions were optimized.4-Aminophenol hydrochloride acted as ultraviolet absorption reagent and eluent.Under the selected chromatographic conditions,the three alkyl sulfonates can be completely separated and detected in 15 min.The detection limits of octane-1-sulfonate,decane-1-sulfonate and dodecyl-1-sulfonate were0.13,0.12,0.97 mg/L,respectively.The method was applied to the determination of 84-disinfectants,and the recovery after spiking was more than 91%.The method was accurate and reliable,and could meet the requirement of quantitative analysis.
An approach for the determination of three kinds of alkyl sulfonates(octane-1-sulfonate,decane-1-sulfonate and dodecyl-1-sulfonate)without ultraviolet absorption by reversed-phase chromatography using 4-aminophenol hydrochloride as the background ultraviolet reagent of indirect ultraviolet detection was developed in the paper.The separation and detection of three alkyl sulfonates were achieved by using C18 reversed phase column and 4-aminophenol hydrochloride/acetonitrile as mobile phases.The effects of ultraviolet absorption reagents,organic solvents,4-aminophenol hydrochloride concentration and the other chromatographic conditions on the separation and detection of alkyl sulfonates were investigated.The retention rules of alkyl sulfonates were discussed and the chromatographic conditions were optimized.4-Aminophenol hydrochloride acted as ultraviolet absorption reagent and eluent.Under the selected chromatographic conditions,the three alkyl sulfonates can be completely separated and detected in 15 min.The detection limits of octane-1-sulfonate,decane-1-sulfonate and dodecyl-1-sulfonate were0.13,0.12,0.97 mg/L,respectively.The method was applied to the determination of 84-disinfectants,and the recovery after spiking was more than 91%.The method was accurate and reliable,and could meet the requirement of quantitative analysis.
引文
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[3]CAI Y Z.Fujian Analysis&Testing(蔡佑振.福建分析测试),2004,13(2):1939.
[4]FENG Y,ZHANG R,ZHANG L,YOU W,PENG J,GAO Z N.Journal of Analytical Science(冯芸,张瑞,张龙,犹卫,彭娟,高作宁.分析科学学报),2015,31(1):23.
[5]Zhi Y W,Zhan W,Arowo M,Zou H K,Sun B C,Chu G W,Zhao H,Chen J F.Chemical Engineering and Processing:Process Intensification,2016,110:123.
[6]Alzaga R,Pena A,Ortiz L,Bayona J M.Journal of Chromatography A,2003,999:51.
[7]LI Z B,TAN H,XIE F,et al.Journal of Instrumental Analysis(李占彬,谭红,谢锋,等.分析测试学报),2012,31(4):379.
[8]HAO L P,MIN W,LI H L.Chinese Journal of Health Laboratory Technology(郝莉鹏,闵巍,李宏亮.中国卫生检验杂志),2012,22(8):1776.
[9]QIN Z H,TAN R,YANG C.Chinese Journal of Analysis Laboratory(秦宗会,谭蓉,杨超.分析试验室),2006,25(8):91.
[10]WANG Y J,GUAN S,HAO D,et al.Chinese Journal of Analytical Chemistry(王玉杰,关胜,郝电,等.分析化学),2002,30(12):1455.
[11]TU H Y,WANG J S,CHEN L H,et al.Chinese Journal of Analysis Laboratory(涂红雨,王俊苏,陈丽华,等.分析试验室),2015,34(3):284.
[12]Lu J Y,Ye F G,Huang X X,Wei L Q,Yao D G,Li S Y,Ouyang M,Lai H F.Journal of Separation Science,2017,40(5):1133.
[13]ZHENG X R,MA Y J,YU H.Journal of Instrumental Analysis(郑秀荣,马亚杰,于泓.分析测试学报),2014,33(9):1026.
[14]SHI X L,ZENG X P,LIN S P.China Food Additives(石相莉,曾雪平,林尚鹏.中国食品添加剂),2009,(5):183.
[15]WANG H J,WANG P W,GUAN J C.Physical Testing and Chemical Analysis-Chemical Analysis(王惠敬,王佩维,关景才.理化检验-化学分册),2004,44(10):917.
[16]GUO H T,QIAO W H,FU X M.Chinese Journal of Analytical Chemistry(郭海涛,乔卫红,付新梅.分析化学),2003,31(8):985.