艾灸对应激性胃溃疡大鼠血清及脑中促肾上腺皮质激素释放激素及促肾上腺皮质激素含量的影响
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摘要
目的:观察艾灸对应激性胃溃疡(SGU)大鼠血清及脑中促肾上腺皮质激素释放激素(CRH)、促肾上腺皮质激素(ACTH)含量的影响,探讨艾灸治疗SGU的机制。方法:将大鼠随机分为空白对照组、模型对照组、艾灸治疗组和西药治疗组,每组7只。采用束缚-浸水应激法制备SGU模型。艾灸治疗组灸"中脘"穴、双侧"足三里"穴,每次20min,1次/d,共5d;西药治疗组选用奥美拉唑肠溶片,按0.2mg·kg-1·d-1灌胃给药,共5d。用Guth法测定胃黏膜损伤程度,HE染色观察胃黏膜的病理学改变,ELISA法检测血清、下丘脑中CRH,血清、垂体中ACTH的含量。结果:造模后大鼠胃黏膜损伤指数高于空白对照组(P<0.01),HE染色示大鼠胃黏膜病理改变明显,血清及下丘脑CRH、血清及垂体ACTH含量升高(P<0.01)。与模型对照组比较,艾灸治疗组、西药治疗组大鼠胃黏膜损伤指数均降低(P<0.01,P<0.05),光镜下胃黏膜病变程度减轻,血清及下丘脑CRH、血清及垂体ACTH含量降低(P<0.01,P<0.05)。结论:艾灸可有效逆转SGU引发的胃黏膜损伤,其机制可能与下调血清及脑组织中CRH、ACTH的含量,抑制下丘脑-垂体-肾上腺轴有关。
Objective To observe the effect of moxibustion of"Zhongwan"(CV12)and "Zusanli"(ST36)on histopathological changes of the gastric mucosa and contents of corticotropin-releasing hormone(CRH)and adrenocorticotrophic hormone(ACTH)in the serum,hypothalamus and pituitary tissues in rats with stress-induced gastric ulcer(SGU),so as to reveal its mechanisms underlying improvement of SGU.Methods A total of 28 male SD rats were randomly divided into blank control,model,moxibustion,and medication groups,with 7 rats in each group.The SGU model was established by water immersion restraint stress for 3 h.Moxibustion was applied to"Zhongwan"(CV12)and bilateral"Zusanli"(ST36)for 20 min,once a day for 5 days,and rats of the medication group were treated by gavage of Omeprazole enteric-coated tablets(0.2 mg/kg)once a day for5 days.The gastric mucosal damage index(ulcer index,UI)was measured to assess the injury severity according to Guths methods.Histopathological changes of the gastric mucosa were determined by H.E.staining.The contents of CRH in serum and hypothalamus and ACTH in serum and pituitary gland tissue were assayed by using ELISA.Results Outcomes of H.E.staining showed gastric mucosal epithelia defect,disordered arrangement of glands,obvious mucosal hyperemia and edema,exudation of a large number of red blood cells,swelling of mucosal cells with necrosis of nuclei in the model group.These situations were relatively milder in the moxibustion and medication groups.After modeling,the UI,and the contents of CRH in the serum and hypothalamus,and ACTH in the serum and pituitary tissue were significantly increased in comparison with the blank control group(P<0.01).Following the intervention,the UI,and contents of CRH in the serum and hypothalamus,and ACTH in the serum and pituitary were all down-regulated in both medication and moxibustion groups relevant to the model group(P<0.05,P<0.01).The therapeutic effect of moxibustion was notably superior to that of medication in down-regulating serum CRH and ACTH(P<0.05).No significant differences were found between the medication and moxibustin interventions in lowering the UI,hypothalamic CRH and pituitary ACTH levels(P>0.05).Conclusion Moxibustion can relieve gastric mucosal injury induced by stress in water immersion restraint stress rats,which may be associated with its effects in down-regulating the levels of CRH and ACTH in se-rum,hypothalamus and pituitary tissues(inhibition of activities of hypothalamic-pituitary-adrenocortical axis).
Objective To observe the effect of moxibustion of"Zhongwan"(CV12)and "Zusanli"(ST36)on histopathological changes of the gastric mucosa and contents of corticotropin-releasing hormone(CRH)and adrenocorticotrophic hormone(ACTH)in the serum,hypothalamus and pituitary tissues in rats with stress-induced gastric ulcer(SGU),so as to reveal its mechanisms underlying improvement of SGU.Methods A total of 28 male SD rats were randomly divided into blank control,model,moxibustion,and medication groups,with 7 rats in each group.The SGU model was established by water immersion restraint stress for 3 h.Moxibustion was applied to"Zhongwan"(CV12)and bilateral"Zusanli"(ST36)for 20 min,once a day for 5 days,and rats of the medication group were treated by gavage of Omeprazole enteric-coated tablets(0.2 mg/kg)once a day for5 days.The gastric mucosal damage index(ulcer index,UI)was measured to assess the injury severity according to Guths methods.Histopathological changes of the gastric mucosa were determined by H.E.staining.The contents of CRH in serum and hypothalamus and ACTH in serum and pituitary gland tissue were assayed by using ELISA.Results Outcomes of H.E.staining showed gastric mucosal epithelia defect,disordered arrangement of glands,obvious mucosal hyperemia and edema,exudation of a large number of red blood cells,swelling of mucosal cells with necrosis of nuclei in the model group.These situations were relatively milder in the moxibustion and medication groups.After modeling,the UI,and the contents of CRH in the serum and hypothalamus,and ACTH in the serum and pituitary tissue were significantly increased in comparison with the blank control group(P<0.01).Following the intervention,the UI,and contents of CRH in the serum and hypothalamus,and ACTH in the serum and pituitary were all down-regulated in both medication and moxibustion groups relevant to the model group(P<0.05,P<0.01).The therapeutic effect of moxibustion was notably superior to that of medication in down-regulating serum CRH and ACTH(P<0.05).No significant differences were found between the medication and moxibustin interventions in lowering the UI,hypothalamic CRH and pituitary ACTH levels(P>0.05).Conclusion Moxibustion can relieve gastric mucosal injury induced by stress in water immersion restraint stress rats,which may be associated with its effects in down-regulating the levels of CRH and ACTH in se-rum,hypothalamus and pituitary tissues(inhibition of activities of hypothalamic-pituitary-adrenocortical axis).
引文
[1]徐琦,梁媛,张文将,等.左金丸对应激性胃溃疡大鼠作用机制的研究[J].湖南中医杂志,2013,29(11):124-126.
[2] HOLSBOER F.Stress,hypercortisolism and corticosteroid receptors in depression:implications for therapy[J].J Affect Disord,2011,62(1-2):77-91.
[3]赵丽,江钢辉.艾灸对慢性疲劳综合征模型大鼠的行为及下丘脑-垂体-肾上腺轴激素水平的影响[J].山东中医杂志,2014,33(4):301-303.
[4]马文彬,刘旭光,周海燕,等.择时艾灸对类风湿性关节炎大鼠下丘脑-垂体-肾上腺轴昼夜节律的影响[J].针刺研究,2016,41(2):100-107.
[5]章海凤,谢芳深,龚红斌,等.热敏灸对肠易激综合征模型大鼠HPA轴调节机制的研究[J].中国针灸,2017,37(12):1315-1321.
[6]闫亚南.预针刺对应激性胃溃疡模型大鼠睡眠时间及CRH含量的影响[J].针灸临床杂志,2016,32(3):67-70.
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[8]石剑峰.应激性溃疡防治的研究进展[J].现代预防医学,2013,40(1):166-167.
[9]路翠艳,潘芳.应激反应中HPA轴的中枢调控和免疫调节[J].中国行为医学科学,2003,12(3):353-355.
[10]杨权.下丘脑-垂体-肾上腺皮质轴应激反应的中枢控制[J].生理科学进展,2000,31(3):222-226.
[11]黄雅梅,周仁来,孙智颖,等.人类应激内分泌轴功能状态的测量[J].心理科学进展,2014,22(4):606-617.
[12]贾宝辉,李志刚,时宇静,等.电针对慢性应激模型大鼠行为学及HPA轴相关激素的影响[J].针刺研究,2004,29(4):252-256.
[13]富文俊,敖海清,孙琪,等.逍遥散对慢性应激损伤大鼠促肾上腺皮质激素释放激素表达的影响[J].中国实验方剂学杂志,2011,17(19):216-218.
[14]张莉华,方步武.脑肠轴及其在胃肠疾病发病机制中的作用[J].中国中西医结合外科杂志,2007,13(2):199-201.
[15]冀来喜,闫丽萍,王海军,等.电针保护大鼠急性胃黏膜损伤基本腧穴配伍“胃病方”的筛选[J].针刺研究,2008,33(5):296-300.
[16]杨波,严兴科.胃溃疡大鼠在合募配穴针刺后胃组织的纳升级二维液相色谱[J].世界华人消化杂志,2010,18(22):2355-2358.
[17]董莉莉,刘安国,王军燕,等.合募配穴对应激性胃溃疡大鼠下丘脑促性腺激素释放激素和P物质mRNA表达的影响[J].针刺研究,2013,38(4):291-296.
[18]王朝辉,张娇娇,王富春.不同腧穴配伍防治应激性胃溃疡的效应规律[J].中国针灸,2014,34(2):149-151.
[19]曾纯,严灿,徐志伟,等.促肾上腺皮质激素释放激素受体在应激反应中的作用研究[J].中国药理学通报,2006,22(5):517-520.
[20] CHEN C Y,MILLION M,ADELSON D W,et.al Intracisternal urocortin inhibits vagally stimulated gastric motility in rats:role of CRF(2)[J].Br J Pharmacol,2002,136(2):237-247.
[21]杨萍.预针刺对应激性溃疡大鼠胃粘膜形态与CRH含量影响的实验研究[D].北京:北京中医药大学,2014.
[22] THIAGARAJAH A S,EADES L E,THOMAS P R,et al.GILZ:Glitzing up our understanding of the glucocorticoid receptor in psychopathology[J].Brain Res,2014,1574:60-69.
[23]孙世林,孙忠人,刘敏,等.温针灸减低阿司匹林抗风湿致胃肠道副作用的研究[J].针刺研究,2011,36(1):28-31.
[2] HOLSBOER F.Stress,hypercortisolism and corticosteroid receptors in depression:implications for therapy[J].J Affect Disord,2011,62(1-2):77-91.
[3]赵丽,江钢辉.艾灸对慢性疲劳综合征模型大鼠的行为及下丘脑-垂体-肾上腺轴激素水平的影响[J].山东中医杂志,2014,33(4):301-303.
[4]马文彬,刘旭光,周海燕,等.择时艾灸对类风湿性关节炎大鼠下丘脑-垂体-肾上腺轴昼夜节律的影响[J].针刺研究,2016,41(2):100-107.
[5]章海凤,谢芳深,龚红斌,等.热敏灸对肠易激综合征模型大鼠HPA轴调节机制的研究[J].中国针灸,2017,37(12):1315-1321.
[6]闫亚南.预针刺对应激性胃溃疡模型大鼠睡眠时间及CRH含量的影响[J].针灸临床杂志,2016,32(3):67-70.
[7] GUTH P H,AURES D,PAULSEN G.Topical aspirin plus HCl gastric lesions in the rat.cytoprotective effect of prostaglandin,cimetidine,and probanthine[J].Gastroenterology,1979,76(1):88-93.
[8]石剑峰.应激性溃疡防治的研究进展[J].现代预防医学,2013,40(1):166-167.
[9]路翠艳,潘芳.应激反应中HPA轴的中枢调控和免疫调节[J].中国行为医学科学,2003,12(3):353-355.
[10]杨权.下丘脑-垂体-肾上腺皮质轴应激反应的中枢控制[J].生理科学进展,2000,31(3):222-226.
[11]黄雅梅,周仁来,孙智颖,等.人类应激内分泌轴功能状态的测量[J].心理科学进展,2014,22(4):606-617.
[12]贾宝辉,李志刚,时宇静,等.电针对慢性应激模型大鼠行为学及HPA轴相关激素的影响[J].针刺研究,2004,29(4):252-256.
[13]富文俊,敖海清,孙琪,等.逍遥散对慢性应激损伤大鼠促肾上腺皮质激素释放激素表达的影响[J].中国实验方剂学杂志,2011,17(19):216-218.
[14]张莉华,方步武.脑肠轴及其在胃肠疾病发病机制中的作用[J].中国中西医结合外科杂志,2007,13(2):199-201.
[15]冀来喜,闫丽萍,王海军,等.电针保护大鼠急性胃黏膜损伤基本腧穴配伍“胃病方”的筛选[J].针刺研究,2008,33(5):296-300.
[16]杨波,严兴科.胃溃疡大鼠在合募配穴针刺后胃组织的纳升级二维液相色谱[J].世界华人消化杂志,2010,18(22):2355-2358.
[17]董莉莉,刘安国,王军燕,等.合募配穴对应激性胃溃疡大鼠下丘脑促性腺激素释放激素和P物质mRNA表达的影响[J].针刺研究,2013,38(4):291-296.
[18]王朝辉,张娇娇,王富春.不同腧穴配伍防治应激性胃溃疡的效应规律[J].中国针灸,2014,34(2):149-151.
[19]曾纯,严灿,徐志伟,等.促肾上腺皮质激素释放激素受体在应激反应中的作用研究[J].中国药理学通报,2006,22(5):517-520.
[20] CHEN C Y,MILLION M,ADELSON D W,et.al Intracisternal urocortin inhibits vagally stimulated gastric motility in rats:role of CRF(2)[J].Br J Pharmacol,2002,136(2):237-247.
[21]杨萍.预针刺对应激性溃疡大鼠胃粘膜形态与CRH含量影响的实验研究[D].北京:北京中医药大学,2014.
[22] THIAGARAJAH A S,EADES L E,THOMAS P R,et al.GILZ:Glitzing up our understanding of the glucocorticoid receptor in psychopathology[J].Brain Res,2014,1574:60-69.
[23]孙世林,孙忠人,刘敏,等.温针灸减低阿司匹林抗风湿致胃肠道副作用的研究[J].针刺研究,2011,36(1):28-31.