EMS诱变刺荞的形态突变体鉴定与分析
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摘要
为构建苦荞EMS突变体库和为苦荞功能基因组学研究准备基础材料,利用化学诱变剂甲基磺酸乙酯(ethyl methane sulfonate,EMS)诱变处理刺荞种子,对获得的M2代材料进行生物学性状与农艺性状鉴定,并对部分M2代材料播种家系进行验证。对M2代全生育期田间表型进行观察鉴定,共获得480份突变体材料,包括子粒、叶片、茎秆及生育期等生物学特性与主要农艺性状突变体,变异类型丰富,尤其是首次发现了子粒多棱苦荞新材料,并发现了自然突变中少见的变异类型,如子粒开裂等变异类型。所构建的刺荞EMS突变群体较理想,可望有效用于苦荞功能基因组研究和苦荞遗传改良。
The mutant library provide the basic material of functional genomics.In this study,seeds of tartary buckwheat Ciqiao was treated by chemical mutagen Ethyl methane sulfonate(EMS).Mutation plants were screened in M2 generation in terms of morphology.Some of mutants identified from M2 plants were validated through M3 generation.The 480 mutant individuals were obtained including grain,leaf,and stem mutants,mature period were obtained with the comparison to the wild type.Some of them were related to important agronomic traits and biological characters,specifically,the mutant of polygonal grain was the first discovered and some mutants were rarely observed in natural variation,such as grain cracking.The mutant population of Ciqiao induced with EMS were ideal mutant population and they would be efficiently used in functional genome research and genetic improvement of tartary buckwheat.
The mutant library provide the basic material of functional genomics.In this study,seeds of tartary buckwheat Ciqiao was treated by chemical mutagen Ethyl methane sulfonate(EMS).Mutation plants were screened in M2 generation in terms of morphology.Some of mutants identified from M2 plants were validated through M3 generation.The 480 mutant individuals were obtained including grain,leaf,and stem mutants,mature period were obtained with the comparison to the wild type.Some of them were related to important agronomic traits and biological characters,specifically,the mutant of polygonal grain was the first discovered and some mutants were rarely observed in natural variation,such as grain cracking.The mutant population of Ciqiao induced with EMS were ideal mutant population and they would be efficiently used in functional genome research and genetic improvement of tartary buckwheat.
引文
[1]Chen Q F.Discussion on the origin of cultivated buckwheat in genus Fagopyrum.Advances in Buckwheat Research,2001:206-213.
[2]林汝法.苦荞举要.北京:中国农业科学技术出版社,2013:3-12.
[3]Chen Q F.A study of resource of Fagopyrum(Polygonaceae)native to China.Bitanical Jourrnal of the Linnean Society,1999,130(1):53-64.
[4]张丽君,马名川,崔林,等.山西省苦荞品种资源的研究.河北农业科学,2015,19(1):69-74.
[5]姜涛,孔令聪,王光宇,等.安徽省苦荞麦种质资源引种观察及鉴定.农学学报,2013,3(7):8-10.
[6]侯雅君,张宗文,吴斌,等.苦荞种质资源AFLP标记遗传多样性分析.中国农业科学,2009,42(12):4166-4174.
[7]马名川,刘龙龙,崔林,等.荞麦育种研究进展.山西农业科学,2015,43(2):240-243.
[8]张晓勤,薛大伟,周伟辉,等.用甲基磺酸乙酯(EMS)诱变的大麦浙农大3号突变体的筛选和鉴定.浙江大学学报(农业与生命科学版),2011,37(2):169-174.
[9]赵天祥,孔秀英,贾继增,等.EMS诱变六倍体小麦偃展4110的形态突变体鉴定与分析.中国农业科学,2009,42(3):755-764.
[10]Wu C Y,Li X J,Yuan W Y.Development of enhancer trap lines for functional analysis of two rice genome.The Plant Journal,2003,35(3):418-427.
[11]张娜,任长忠,胡银岗,等.白燕2号EMS突变体的形态鉴定与遗传变异分析.麦类作物学报,2011,31(3):421-426.
[12]黄冬福,付文婷,何建文,等.农作物EMS诱变研究进展.北方园艺,2015(24):188-194.
[13]卢银,刘梦洋,申书新,等.EMS处理对大白菜种子和幼苗活力的影响及M2表型变异分析.植物遗传资源学报,2015,16(2):349-358.
[14]Chiu W H,Chandler J,Cnops G,et al.Mutations in the TORNADO2gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana.Plant Molecular Biology,2007,63(6):731-744.
[15]Ray S K,Rajeshwari R,Sonti R V.Mutants of Xanthomonas oryzae pv.oryzae deficient in general secretory pathway are virulence deficient and unable to secrete xylanase.Molecular Plant-Microbe Interactions,2000,13(4):394-401.
[16]陈洋,高兰英,邵艳军,等.EMS诱导小麦易位系YW642突变体的鉴定与分子标记分析.核农学报,2011,25(4):617-621.
[17]姜昱,李毅丹,刘相国,等.EMS诱变技术在我国玉米育种中的研究与应用.吉林农业科学,2012,37(6):21-24.
[18]韩锁义,张恒友,杨玛丽,等.大豆“南农86-4”突变体筛选及突变体库的构建.作物学报,2007,33(12):2059-2062.
[19]Caldwell D G,McCallum N,Shaw P,et al.A structured mutant population for forward and reverse genetics in barley.The Plant Journal,2004,40(1):143-150.
[20]张宏军,肖钢,谭太龙,等.EMS处理甘蓝型油菜(Brassica napus)获得高油酸材料.中国农业科学,2008,41(12):4016-4022.
[21]李国柱,董艳辉,申慧芳,等.苦荞辐照突变体黄酮含量与PAL活性和农艺性状研究.激光生物学报,2010,19(4):546-551.
[22]Yao Y N,Xuan Z Y,He Y M,et al.Principal component analysis of intraspecific responses of tartary buckwheat to UV-B radiation under field conditions.Environmental and Experimental Botany,2007,61(3):237-245.
[23]郭耀东,温日宇,武小平,等.不同浓度EMS对苦荞种子发芽的影响.安徽农业科学,2014,42(26):8938-8939.
[24]邓琳琼,张以忠.苦荞EMS诱变群体的创建及农艺性状分析.湖北农业科学,2015,54(14):3343-3347.
[25]Chen Q F.Wide hybridization among Fagopyrum(Polygonaceae)native to China.Botanical Journal of the Linnean Society,1999,131:177-185.
[26]张以忠,邓琳琼.EMS诱变苦荞突变体与其亲本的比较分析.热带作物学报,2017,38(9):1601-1606.
[27]王安虎,戴红燕,邓建平.60Co-γ射线辐射苦荞麦籽粒M2至M5代变异性状不稳定性研究.杂粮作物,2005,25(6):364-366.
[28]徐艳花,陈锋,董中东,等.EMS诱变的普通小麦豫农201突变体库的构建与初步分析.麦类作物学报,2010,30(4):625-629.
[29]Zhang L J,Cui L,Qiao Z J,et al.The tartary buckwheat genome provides insights into rutin biosynthesis and abiotic stress tolerance.Molecular Plant,2017,10(9):1224-1237.
[2]林汝法.苦荞举要.北京:中国农业科学技术出版社,2013:3-12.
[3]Chen Q F.A study of resource of Fagopyrum(Polygonaceae)native to China.Bitanical Jourrnal of the Linnean Society,1999,130(1):53-64.
[4]张丽君,马名川,崔林,等.山西省苦荞品种资源的研究.河北农业科学,2015,19(1):69-74.
[5]姜涛,孔令聪,王光宇,等.安徽省苦荞麦种质资源引种观察及鉴定.农学学报,2013,3(7):8-10.
[6]侯雅君,张宗文,吴斌,等.苦荞种质资源AFLP标记遗传多样性分析.中国农业科学,2009,42(12):4166-4174.
[7]马名川,刘龙龙,崔林,等.荞麦育种研究进展.山西农业科学,2015,43(2):240-243.
[8]张晓勤,薛大伟,周伟辉,等.用甲基磺酸乙酯(EMS)诱变的大麦浙农大3号突变体的筛选和鉴定.浙江大学学报(农业与生命科学版),2011,37(2):169-174.
[9]赵天祥,孔秀英,贾继增,等.EMS诱变六倍体小麦偃展4110的形态突变体鉴定与分析.中国农业科学,2009,42(3):755-764.
[10]Wu C Y,Li X J,Yuan W Y.Development of enhancer trap lines for functional analysis of two rice genome.The Plant Journal,2003,35(3):418-427.
[11]张娜,任长忠,胡银岗,等.白燕2号EMS突变体的形态鉴定与遗传变异分析.麦类作物学报,2011,31(3):421-426.
[12]黄冬福,付文婷,何建文,等.农作物EMS诱变研究进展.北方园艺,2015(24):188-194.
[13]卢银,刘梦洋,申书新,等.EMS处理对大白菜种子和幼苗活力的影响及M2表型变异分析.植物遗传资源学报,2015,16(2):349-358.
[14]Chiu W H,Chandler J,Cnops G,et al.Mutations in the TORNADO2gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana.Plant Molecular Biology,2007,63(6):731-744.
[15]Ray S K,Rajeshwari R,Sonti R V.Mutants of Xanthomonas oryzae pv.oryzae deficient in general secretory pathway are virulence deficient and unable to secrete xylanase.Molecular Plant-Microbe Interactions,2000,13(4):394-401.
[16]陈洋,高兰英,邵艳军,等.EMS诱导小麦易位系YW642突变体的鉴定与分子标记分析.核农学报,2011,25(4):617-621.
[17]姜昱,李毅丹,刘相国,等.EMS诱变技术在我国玉米育种中的研究与应用.吉林农业科学,2012,37(6):21-24.
[18]韩锁义,张恒友,杨玛丽,等.大豆“南农86-4”突变体筛选及突变体库的构建.作物学报,2007,33(12):2059-2062.
[19]Caldwell D G,McCallum N,Shaw P,et al.A structured mutant population for forward and reverse genetics in barley.The Plant Journal,2004,40(1):143-150.
[20]张宏军,肖钢,谭太龙,等.EMS处理甘蓝型油菜(Brassica napus)获得高油酸材料.中国农业科学,2008,41(12):4016-4022.
[21]李国柱,董艳辉,申慧芳,等.苦荞辐照突变体黄酮含量与PAL活性和农艺性状研究.激光生物学报,2010,19(4):546-551.
[22]Yao Y N,Xuan Z Y,He Y M,et al.Principal component analysis of intraspecific responses of tartary buckwheat to UV-B radiation under field conditions.Environmental and Experimental Botany,2007,61(3):237-245.
[23]郭耀东,温日宇,武小平,等.不同浓度EMS对苦荞种子发芽的影响.安徽农业科学,2014,42(26):8938-8939.
[24]邓琳琼,张以忠.苦荞EMS诱变群体的创建及农艺性状分析.湖北农业科学,2015,54(14):3343-3347.
[25]Chen Q F.Wide hybridization among Fagopyrum(Polygonaceae)native to China.Botanical Journal of the Linnean Society,1999,131:177-185.
[26]张以忠,邓琳琼.EMS诱变苦荞突变体与其亲本的比较分析.热带作物学报,2017,38(9):1601-1606.
[27]王安虎,戴红燕,邓建平.60Co-γ射线辐射苦荞麦籽粒M2至M5代变异性状不稳定性研究.杂粮作物,2005,25(6):364-366.
[28]徐艳花,陈锋,董中东,等.EMS诱变的普通小麦豫农201突变体库的构建与初步分析.麦类作物学报,2010,30(4):625-629.
[29]Zhang L J,Cui L,Qiao Z J,et al.The tartary buckwheat genome provides insights into rutin biosynthesis and abiotic stress tolerance.Molecular Plant,2017,10(9):1224-1237.