电针大鼠背俞穴降糖作用的实验研究
|
摘要
目的:观察电针不同穴位对2型糖尿病模型大鼠的空腹血糖、口服葡萄糖耐受的影响,验证电针的降糖作用。方法:以100只雄性SD大鼠为研究对象,随机抽取13只为空白组,其余以高糖高脂饲料喂养结合小剂量链脲佐菌素(streptozotocin,STZ)腹腔注射制备2型糖尿病大鼠模型。成模大鼠60只随机分为模型组、胃脘下俞组、心俞组、肾俞组、后三里组,每组12只。胃脘下俞组、心俞组、肾俞组、后三里组均以改善饮食习性结合频率2 Hz、强度2mA的电针干预,分别刺激"胃脘下俞""心俞""肾俞""后三里",6次/周,共干预4周。空白组和模型组不予干预。以干预第7、14、21、28d空腹血糖,干预第21d电针即刻降糖效应、干预结束后口服葡萄糖耐量试验(oral glucose tolerance test,OGTT)曲线下面积为指标,观察各组血糖调控情况。结果:以高糖高脂饮食结合小剂量STZ腹腔注射的造模方法能很好地模拟2型糖尿病高血糖状况。与干预前相比,胃脘下俞组、心俞组、肾俞组血糖在第1周或第2周末显著降低(P<0.05,P<0.01);第3周末电针干预后,胃脘下俞组及心俞组即刻空腹血糖显著低于模型组(均P<0.01)及后三里组(均P<0.01);胃脘下俞组OGTT曲线下面积显著低于模型组(P<0.05),其余各干预组与模型组比较差异无统计学意义(均P>0.05)。结论:以低频电针刺激"胃脘下俞""心俞""肾俞"均具有降糖作用,其起效时间、效果强度、效果持续时间有所不同,以"胃脘下俞"的降血糖作用效果更显著。
Objective To observe the effects of electroacupuncture(EA)at different acupoints for fasting blood glucose(FBG)and oral glucose tolerance in type 2diabetes rats so as to verify the glucose-lowering effects of EA.Methods Total 100 SD male rats were seen as experimental objects,among which 13 were randomly assigned into a blank group.Other rats were fed with high fat and high sugar diet combined with intraperitoneal injection of small dose streptozotocin(STZ)to establish type 2diabetes model.Sixty diabetic rats were randomly assigned into a model group,a Weiwanxiashu group,a Xinshu group,a Shenshu group and a Housanli group,12 cases in each one.Combined with the improved diet habits and routines,EA(2 Hz and 2 mA)was used at"Weiwanxiashu"(EX-B 3),"Xinshu"(BL 15),"Shenshu"(BL 23)and"Housanli"(ST 36)in the corresponding groups,6times a week for 4weeks.There was no treatment in the blank group and in the model group.The observation indexes were the FBG on the 7th,14 th,21st,and 28 th day of intervention,the instant glucose-lowering effect on the 21 st day during treatment,and the area under the curve of oral glucose tolerance test(OGTT)after intervention.Also,the glucose regulation condition was observed.Results Type 2diabetes model could be established by high fat and high sugar diet combined with intraperitoneal injection of small dose STZ.Glucose decreased apparently at the end of the 1st week or the 2nd week compared with that before treatment in the Weiwanxiashu,Xinshu and Shenshu groups(P<0.05,P<0.01).The instant FBG of the Weiwanxiashu and Xinshu groups was obviously lower than that of the model and Housanli groups at the end of the 3rd week(all P<0.01).The area under the curve of OGTT of the Weiwanxiashu group was apparently smaller than that of the model group(P<0.05),and the results of the index in the other groups were not significantly different from that of the model group(all P>0.05).Conclusion Low frequency EA at"Weiwanxiashu"(EX-B 3),"Xinshu"(BL 15)and"Shenshu"(BL 23)can reduce glucose with different onset times,effects,and durations.And"Weiwanxiashu"(EX-B 3)is more effective.
Objective To observe the effects of electroacupuncture(EA)at different acupoints for fasting blood glucose(FBG)and oral glucose tolerance in type 2diabetes rats so as to verify the glucose-lowering effects of EA.Methods Total 100 SD male rats were seen as experimental objects,among which 13 were randomly assigned into a blank group.Other rats were fed with high fat and high sugar diet combined with intraperitoneal injection of small dose streptozotocin(STZ)to establish type 2diabetes model.Sixty diabetic rats were randomly assigned into a model group,a Weiwanxiashu group,a Xinshu group,a Shenshu group and a Housanli group,12 cases in each one.Combined with the improved diet habits and routines,EA(2 Hz and 2 mA)was used at"Weiwanxiashu"(EX-B 3),"Xinshu"(BL 15),"Shenshu"(BL 23)and"Housanli"(ST 36)in the corresponding groups,6times a week for 4weeks.There was no treatment in the blank group and in the model group.The observation indexes were the FBG on the 7th,14 th,21st,and 28 th day of intervention,the instant glucose-lowering effect on the 21 st day during treatment,and the area under the curve of oral glucose tolerance test(OGTT)after intervention.Also,the glucose regulation condition was observed.Results Type 2diabetes model could be established by high fat and high sugar diet combined with intraperitoneal injection of small dose STZ.Glucose decreased apparently at the end of the 1st week or the 2nd week compared with that before treatment in the Weiwanxiashu,Xinshu and Shenshu groups(P<0.05,P<0.01).The instant FBG of the Weiwanxiashu and Xinshu groups was obviously lower than that of the model and Housanli groups at the end of the 3rd week(all P<0.01).The area under the curve of OGTT of the Weiwanxiashu group was apparently smaller than that of the model group(P<0.05),and the results of the index in the other groups were not significantly different from that of the model group(all P>0.05).Conclusion Low frequency EA at"Weiwanxiashu"(EX-B 3),"Xinshu"(BL 15)and"Shenshu"(BL 23)can reduce glucose with different onset times,effects,and durations.And"Weiwanxiashu"(EX-B 3)is more effective.
引文
[1]Danaei G,Finucane MM,LU Y,et al.National,regional,and global trends in fasting plasma glucose and diabetes prevalence since 1980:systamatic analysis of health examination surveys and epidemiological studies with 370 country-years and 2.7 million participants[J].Lancet,2011,378(9785):31-40.
[2]Guariguata L,Whiting DR,Hambleton I,et al.Global estimates of diabetes prevalence for 2013and projections for 2035for the IDF diabetes atlas[J].Diabetes Res Clin Pract,2014,103(2):137-149.
[3]World Health Organization.Acupuncture:review and analysis of reports on controlled clinical trials[M].Geneva:World Health Organization,2003.
[4]高珊,李瑞,田环环.胃脘下俞穴的古今研究[J].上海针灸杂志,2014,33(12):1163-1166.
[5]张露芬.实验针灸学[M].北京:化学工业出版社,2010:219-220.
[6]田环环,曹昺焱,高珊,等.大鼠肾俞定位之我见[J].针灸临床杂志,2014,30(1):51-52.
[7]刘万里,薛茜,曹明芹,等.用SPSS实现完全随机设计多组比较秩和检验的多重比较[J].地方病通报,2007,22(2):27-29.
[8]李忠仁.实验针灸学[M].北京:中国中医药出版社,2007.
[9]崔承斌,王京京,吴中朝.从背俞穴与夹脊穴的关系论背俞功能带[J].中国针灸,2005,25(7):483-486.
[10]赫增才.论十四经穴中的第362个穴位——胰俞[J].中国针灸,2007,27(6):474.
[11]沈雪勇.经络腧穴学[M].北京:中国中医药出版社,2003.
[12]王媛,刘志诚,徐斌.针刺治疗女性2型糖尿病疗效分析[J].中国针灸,2014,34(1):21-24.
[13]高珊,李瑞,田环环,等.电针“胰俞”穴对2型糖尿病大鼠HPA轴相关激素的影响[J].中国针灸,2014,34(11):1099-1105.
[2]Guariguata L,Whiting DR,Hambleton I,et al.Global estimates of diabetes prevalence for 2013and projections for 2035for the IDF diabetes atlas[J].Diabetes Res Clin Pract,2014,103(2):137-149.
[3]World Health Organization.Acupuncture:review and analysis of reports on controlled clinical trials[M].Geneva:World Health Organization,2003.
[4]高珊,李瑞,田环环.胃脘下俞穴的古今研究[J].上海针灸杂志,2014,33(12):1163-1166.
[5]张露芬.实验针灸学[M].北京:化学工业出版社,2010:219-220.
[6]田环环,曹昺焱,高珊,等.大鼠肾俞定位之我见[J].针灸临床杂志,2014,30(1):51-52.
[7]刘万里,薛茜,曹明芹,等.用SPSS实现完全随机设计多组比较秩和检验的多重比较[J].地方病通报,2007,22(2):27-29.
[8]李忠仁.实验针灸学[M].北京:中国中医药出版社,2007.
[9]崔承斌,王京京,吴中朝.从背俞穴与夹脊穴的关系论背俞功能带[J].中国针灸,2005,25(7):483-486.
[10]赫增才.论十四经穴中的第362个穴位——胰俞[J].中国针灸,2007,27(6):474.
[11]沈雪勇.经络腧穴学[M].北京:中国中医药出版社,2003.
[12]王媛,刘志诚,徐斌.针刺治疗女性2型糖尿病疗效分析[J].中国针灸,2014,34(1):21-24.
[13]高珊,李瑞,田环环,等.电针“胰俞”穴对2型糖尿病大鼠HPA轴相关激素的影响[J].中国针灸,2014,34(11):1099-1105.