海洋放线菌天然活性产物的发掘
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摘要
海洋微生物生活在高盐、高压、低温、低营养或无光照等特殊生境,造就了其种类的多样性和特殊性,能产生海洋微生物所特有的结构新颖、作用特殊的生物活性物质。在海洋微生物中放线菌具有较为复杂的发育分化过程,还能产生大量次级代谢产物。该文综述了近年来从海洋放线菌中筛选天然活性化合物的有效方法,包括优化培养基,改进培养方法和检测技术,运用基因组挖掘技术等。这些有效方法的运用使得从海洋放线菌中筛选生物活性物质具有广阔的开发应用前景。
Marine microorganisms live in special habitats such as high salt, high pressure, low temperature, oligotrophic or no light, which resulted in the diversity and specificity of their species, and can produce the marine derived bioactive compounds with novel chemical structure and specific functions. Among marine microorganism, Actinomycetes have a relatively complex process of development and differentiation, and can also produce a great variety of secondary metabolites. The effective methods of screening natural active compounds from marine Actinomycetes in recent years, including optimizing the medium, improving the culture methods and detection technology, and applying genome mining technology were reviewed.The application of these effective methods made the screening of bioactive substances from marine Actinomycetes had a broad prospect of development and application.
Marine microorganisms live in special habitats such as high salt, high pressure, low temperature, oligotrophic or no light, which resulted in the diversity and specificity of their species, and can produce the marine derived bioactive compounds with novel chemical structure and specific functions. Among marine microorganism, Actinomycetes have a relatively complex process of development and differentiation, and can also produce a great variety of secondary metabolites. The effective methods of screening natural active compounds from marine Actinomycetes in recent years, including optimizing the medium, improving the culture methods and detection technology, and applying genome mining technology were reviewed.The application of these effective methods made the screening of bioactive substances from marine Actinomycetes had a broad prospect of development and application.
引文
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[8]程亮,罗明明,吴继纲,等.海洋放线菌Y12-26中抗真菌活性代谢产物的分离纯化与结构鉴定[J].中国抗生素杂志,2017,42(8):631-638.
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[10]姜钊,职晓阳,李文均.分离自印度洋深海沉积物的部分链霉菌分离株多位点序列分析[J].微生物学通报,2016,43(5):927-934.
[11]徐洪凯,闫克强,何燕斌,等.宏蛋白质组学信息分析的基本策略及其挑战[J].生物化学与生物物理进展,2018,45(1):23-35.
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[15]OBERHARDT M A,ZARECKI R,GRONOW S,et al.Harnessing the landscape of microbial culture media to predict new organism-media pairings[J].Nature Communicat,2015,6:8493.
[16]EBADA S S,FISCHER T,KLA魨譔EN S,et al.A newcytotoxic steroid from co-fermentation of two marine alga-derived micro-organisms[J].Nat Prod Res,2014,28(16):1241-1245.
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[20]EILERS H,PERNTHALER J,PEPLIES J,et al.Isolation of novel pelagic bacteria from the German bight and their seasonal contributions to surface picoplankton[J].Appl Environ Microbiol,2001,67(11):5134-5142.
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[22]SCHUT F,PRINS R A,GOTTSCHAL J C.Oligotrophy and pelagic marine bacteria:facts and fiction[J].Aquat Microb Ecol,1997,12(2):177-202.
[23]LIU W,KIM H J,LUCCHETTA E M,et al.Isolation,incubation,and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement[J].Lab on A Chip,2009,9(15):2153-2162.
[24]PORTER J,EDWARDS C,MORGAN J A,et al.Rapid,automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting[J].Appl Environ Microbiol,1993,59(10):3327-3333.
[25]KVIST T,AHRING B K,LASKEN R S,et al.Specific single-cell isolation and genomic amplification of uncultured microorganisms[J].Appl Microbiol Biot,2006,74(4):926-935.
[26]ESQUENAZI E,YANG Y L,WATROUS J,et al.Imaging mass spectrometry of natural products[J].Nat Prod Report,2009,26(12):1521-1534.
[27]BARGER S R,HOEFLER B C,CUBILLOSRUIZ A,et al.Imaging secondary metabolism of Streptomyces sp.Mg1 during cellular lysis and colony degradation of competing Bacillus subtilis[J].Anton Leeuw,2012,102(3):435-445.
[28]LIU W T,KERSTEN R D,YANG Y L,et al.Imaging mass spectrometry and genome mining via short sequence tagging identified the anti-infective agent arylomycin in Streptomyces roseosporus[J].J Am Chem Soc,2011,133(45):18010-18013.
[29]ZHAO X Q,JIAO W C,JIANG B,et al.Screening and identification of actinobacteria from marine sediments:Investigation of potential producers for antimicrobial agents and type I polyketides[J].World J Microb Biot,2009,25(5):859-866.
[30]戴欣,周惠,陈月琴,等.中国南海南沙海区沉积物中细菌16S r DNA多样性的初步研究[J].自然科学进展,2002,12(5):479-484.
[31]BERNAL A,EAR U,KYRPIDES N.Genomes On Line Database(GOLD):a monitor of genome projects world-wide[J].Nucl Acid Res,2001,29(1):126-127.
[32]GALV魨￡O T C,MOHN W W,DE L V.Exploring the microbial biodegradation and biotransformation gene pool[J].Trend Biotechnol,2005,23(10):497-506.
[33]HORNUNG A,BERTAZZO M,DZIARNOWSKI A,et al.A genomic screening approach to the structure-guided identification of drug candidates from natural sources[J].Chem Biochem,2007,8(7):757-766.
[34]CHEN F F,LIN L,WANG Y G,et al.Distribution of d TDP-glucose-4,6-dehydratase gene and diversity of potential glycosylated natural products in marine sediment-derived bacteria[J].Appl Microbiol Biot,2011,90(4):1347-1359.
[35]黄胜,李娜,周俊,等.适用于链霉菌大片段基因组DNA克隆和异源表达的细菌人工染色体(BAC)载体的构建及应用[J].微生物学报,2012,52(1):30-37.
[36]BRADY S F,CHAO C J,HANDELSMAN J,et al.Cloning and heterologous expression of a natural product biosynthetic gene cluster from e DNA[J].Organ Lett,2001,3(13):1981-1984.
[37]SALOMON C E,MAGARVEY N A,SHERMAN D H.Merging the potential of microbial genetics with biological and chemical diversity:an even brighter future for marine natural product drug discovery[J].Nat Prod Report,2004,21(1):105-121.
[2]王艺颖,丛丽娜,王伟,等.产生物活性物质海洋放线菌的筛选和鉴定[J].工业微生物,2017,47(3):30-35.
[3]SUBRAMANI R,AALBERSBERG W.Culturable rare Actinomycetes:diversity,isolation and marine natural product discovery[J].Appl Microbiol Biotechnol,2013,97(21):9291-9321.
[4]NEWMAN D J,CRAGG G M.Current status of marine-derived compounds as warheads in anti-tumor drug candidates[J].Marine Drugs,2017,15(4):99.
[5]杜鹃,谢峻,郑颖城,等.《抗生素耐药:全球监测报告2014》解读与反思[J].华南国防医学杂志,2014,28(8):814-817.
[6]王磊,曹巍.全球抗生素耐药性现状分析及对策建议[J].军事医学,2017,41(5):329-333.
[7]穆大帅,卢德臣,郑维爽,等.我国海洋细菌新物种鉴定与资源研发进展[J].生物资源,2017,39(6):391-397.
[8]程亮,罗明明,吴继纲,等.海洋放线菌Y12-26中抗真菌活性代谢产物的分离纯化与结构鉴定[J].中国抗生素杂志,2017,42(8):631-638.
[9]GOODFELLOW M,FIEDLER H P.A guide to successful bioprospecting:informed by actinobacterial systematics[J].Anton Leeuw,2010,98(2):119-142.
[10]姜钊,职晓阳,李文均.分离自印度洋深海沉积物的部分链霉菌分离株多位点序列分析[J].微生物学通报,2016,43(5):927-934.
[11]徐洪凯,闫克强,何燕斌,等.宏蛋白质组学信息分析的基本策略及其挑战[J].生物化学与生物物理进展,2018,45(1):23-35.
[12]SCHLEIFER KH.Microbial diversity:facts,problems and prospects[J].Syst Appl Microbiol,2004,27(1):3-9.
[13]JENSEN P R,GONTANG E,MAFNAS C,et al.Culturable marine Actinomycete diversity from tropical Pacific Ocean sediments[J].Environ Microbiol,2010,7(7):1039-1048.
[14]BRUNS A,CYPIONKA H,OVERMANN J.Cyclic AMP and acyl homoserine lactones increase the cultivation efficiency of heterotrophic bacteria from the central baltic sea[J].Appl Environ Microbiol,2002,68(8):3978-3987.
[15]OBERHARDT M A,ZARECKI R,GRONOW S,et al.Harnessing the landscape of microbial culture media to predict new organism-media pairings[J].Nature Communicat,2015,6:8493.
[16]EBADA S S,FISCHER T,KLA魨譔EN S,et al.A newcytotoxic steroid from co-fermentation of two marine alga-derived micro-organisms[J].Nat Prod Res,2014,28(16):1241-1245.
[17]CHO J Y,KIM M S.Induction of antifouling diterpene production by Streptomyces cinnabarinus PK209 in co-culture with marine-derived Alteromonas sp.KNS-16[J].J Agr Chem Soc Jpn,2012,76(10):1849-1854.
[18]戴欣,王保军,黄燕,等.普通和稀释培养基研究太湖沉积物可培养细菌的多样性[J].微生物学报,2005,45(2):161-165.
[19]AKSELBAND Y,CABRAL C,CASTOR T P,et al.Enrichment of slow-growing marine microorganisms from mixed cultures using gel microdrop(GMD)growth assay and fluorescence-activated cell sorting[J].J Exp Marine Biol Ecol,2006,329(2):196-205.
[20]EILERS H,PERNTHALER J,PEPLIES J,et al.Isolation of novel pelagic bacteria from the German bight and their seasonal contributions to surface picoplankton[J].Appl Environ Microbiol,2001,67(11):5134-5142.
[21]KAEBERLEIN T,LEWIS K,EPSTEIN SS.Isolating"uncultivable"microorganisms in pure culture in a simulated natural environment[J].Science,2002,296(5570):1127-1129.
[22]SCHUT F,PRINS R A,GOTTSCHAL J C.Oligotrophy and pelagic marine bacteria:facts and fiction[J].Aquat Microb Ecol,1997,12(2):177-202.
[23]LIU W,KIM H J,LUCCHETTA E M,et al.Isolation,incubation,and parallel functional testing and identification by FISH of rare microbial single-copy cells from multi-species mixtures using the combination of chemistrode and stochastic confinement[J].Lab on A Chip,2009,9(15):2153-2162.
[24]PORTER J,EDWARDS C,MORGAN J A,et al.Rapid,automated separation of specific bacteria from lake water and sewage by flow cytometry and cell sorting[J].Appl Environ Microbiol,1993,59(10):3327-3333.
[25]KVIST T,AHRING B K,LASKEN R S,et al.Specific single-cell isolation and genomic amplification of uncultured microorganisms[J].Appl Microbiol Biot,2006,74(4):926-935.
[26]ESQUENAZI E,YANG Y L,WATROUS J,et al.Imaging mass spectrometry of natural products[J].Nat Prod Report,2009,26(12):1521-1534.
[27]BARGER S R,HOEFLER B C,CUBILLOSRUIZ A,et al.Imaging secondary metabolism of Streptomyces sp.Mg1 during cellular lysis and colony degradation of competing Bacillus subtilis[J].Anton Leeuw,2012,102(3):435-445.
[28]LIU W T,KERSTEN R D,YANG Y L,et al.Imaging mass spectrometry and genome mining via short sequence tagging identified the anti-infective agent arylomycin in Streptomyces roseosporus[J].J Am Chem Soc,2011,133(45):18010-18013.
[29]ZHAO X Q,JIAO W C,JIANG B,et al.Screening and identification of actinobacteria from marine sediments:Investigation of potential producers for antimicrobial agents and type I polyketides[J].World J Microb Biot,2009,25(5):859-866.
[30]戴欣,周惠,陈月琴,等.中国南海南沙海区沉积物中细菌16S r DNA多样性的初步研究[J].自然科学进展,2002,12(5):479-484.
[31]BERNAL A,EAR U,KYRPIDES N.Genomes On Line Database(GOLD):a monitor of genome projects world-wide[J].Nucl Acid Res,2001,29(1):126-127.
[32]GALV魨￡O T C,MOHN W W,DE L V.Exploring the microbial biodegradation and biotransformation gene pool[J].Trend Biotechnol,2005,23(10):497-506.
[33]HORNUNG A,BERTAZZO M,DZIARNOWSKI A,et al.A genomic screening approach to the structure-guided identification of drug candidates from natural sources[J].Chem Biochem,2007,8(7):757-766.
[34]CHEN F F,LIN L,WANG Y G,et al.Distribution of d TDP-glucose-4,6-dehydratase gene and diversity of potential glycosylated natural products in marine sediment-derived bacteria[J].Appl Microbiol Biot,2011,90(4):1347-1359.
[35]黄胜,李娜,周俊,等.适用于链霉菌大片段基因组DNA克隆和异源表达的细菌人工染色体(BAC)载体的构建及应用[J].微生物学报,2012,52(1):30-37.
[36]BRADY S F,CHAO C J,HANDELSMAN J,et al.Cloning and heterologous expression of a natural product biosynthetic gene cluster from e DNA[J].Organ Lett,2001,3(13):1981-1984.
[37]SALOMON C E,MAGARVEY N A,SHERMAN D H.Merging the potential of microbial genetics with biological and chemical diversity:an even brighter future for marine natural product drug discovery[J].Nat Prod Report,2004,21(1):105-121.