中药miRNA研究进展
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摘要
miRNA是一类由18～24 nt核苷酸构成的非编码内源性小分子RNA,在真核生物细胞分化、增殖与凋亡等转录后水平调控表达,与机体代谢、免疫、肿瘤等生理病理过程有密切联系,为疾病的诊断与治疗带来新思路。中药中miRNA可通过消化道进入机体血液和组织器官,在疾病治疗中发挥重要作用,在创新中药新药方面的意义值得深入探索。可以通过small RNA建库测序或microRNA芯片技术对中药miRNA进行鉴定、分析和应用研究。
miRNAs are a group of non-coding endogenous small molecule RNAs,which are composed of 18 ～ 24 nt nucleotides. They are expressed in eukaryotic cells after transcriptional differentiation,proliferation and apoptosis. miRNAs are closely related to the pathophysiological processes such as metabolism,tumor,and immunity and so on. They also bring new intervention pathways and targets for the treatment and diagnosis of diseases.miRNAs can enter the body's blood,tissues and organs through the digestive tract and play an important role in the treatment of diseases,which are expected to become a new mechanism for the efficacy of traditional Chinese medicines. They are worthy of further exploration and analysis for innovating new drugs of traditional Chinese medicines. We can identify,analyze and utilize the miRNAs in traditional Chinese medicines through small RNA library sequencing or microRNA chip technology.
miRNAs are a group of non-coding endogenous small molecule RNAs,which are composed of 18 ～ 24 nt nucleotides. They are expressed in eukaryotic cells after transcriptional differentiation,proliferation and apoptosis. miRNAs are closely related to the pathophysiological processes such as metabolism,tumor,and immunity and so on. They also bring new intervention pathways and targets for the treatment and diagnosis of diseases.miRNAs can enter the body's blood,tissues and organs through the digestive tract and play an important role in the treatment of diseases,which are expected to become a new mechanism for the efficacy of traditional Chinese medicines. They are worthy of further exploration and analysis for innovating new drugs of traditional Chinese medicines. We can identify,analyze and utilize the miRNAs in traditional Chinese medicines through small RNA library sequencing or microRNA chip technology.
引文
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[27]向静,黄洁嫦,徐畅,等.甘草水提物中miRNA对人免疫细胞基因表达的影响[J].中国中药杂志,2017,42(9):1752-1756.
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[29]ZHU K,LIU M,FU Z,et al.Plant microRNAs in larval food regulate honeybee caste development[J].Plos Genetics,2017,13(8):e1006946.
[30]SHAHID S,KIM G,JOHNSON NR,et al.MicroRNAs from the parasitic plant Cuscutacampestris target host messenger RNAs[J].Nature,2018,553(7686):82-85.
[31]吴望军,陈杰,黄瑞华,等.高通量测序技术挖掘猪microRNA的研究进展[J].畜牧兽医学报,2013,44(12):1857-1866.
[32]ARATA H,KOMATSU H,HAN A,et al.Rapid microRNA detection using power-free microfluidic chip:coaxial stacking effect enhances the sandwich hybridization[J].Analyst,2012,137(14):3234-3237.
[2]陈凌燕,孙兰军,徐强.microRNA对心力衰竭调控作用的研究进展[J].中华中医药学刊,2014,32(7):1627-1630.
[3]JUNG M,SCHAEFER A,STEINER I,et al.Robust microRNAstability in degraded RNA preparations from human tissue and cell samples[J].Clin Chem,2010,56(6):998-1006.
[4]DRUMMOND MJ,MCCARTHY JJ,SINHA M,et al.Aging and microRNA expression in human skeletal muscle:a microarray and bioinformatics analysis[J].Physiological Genomics,2011,43(10):595.
[5]ABDELMOHSEN K,SRIKANTAN S,KANG MJ,et al.Regulation of senescence by microRNA biogenesis factors[J].Ageing Res Rev,2012,11(4):491-500.
[6]JIANG YW,CHEN LA.MicroRNAs as tumor inhibitors,oncogenes,biomarkers for drug efficacy and outcome predictors in lung cancer(Review)[J].Mol Med Report,2012,5(4):890-894.
[7]ZEN K,ZHANG CY.Circulating MicroRNAs:a novel class of biomarkers to diagnose and monitor human cancers[J].Med Res Rev,2012,32(2):326-348.
[8]YANG YH,CHEN XJ,CHEN JY,et al.Differential miRNAexpression in Rehmanniaglutinosa plants subjected to continuous cropping[J].BMC Plant Biology,2011,11(1):53-63.
[9]王颖芳,王宇亮,韩彬,等.人参中miRNA的提取和鉴定分析[J].实用中医内科杂志,2012,26(9):7-8,10.
[10]王颖芳,陈艳琳,王文娟.适用于转录组测序的人参根总RNA提取方法的筛选[J].广东药科大学学报,2017,33(1):18-22.
[11]吕帝瑾,陈婧,钟扬,等.两种提取蒙古黄芪miRNA方法的比较[J].植物生理学报,2014(8):1255-1258
[12]KALINOWSKA E,CHODORSKA M,PADUCH-CICHAL E,et al.An improved method for RNA isolation from plants using commercial extraction kits[J].Acta Biochimica Polonica,2012,59(3):391-393.
[13]ACCERBIM,SCHMIDT SA,DE PE,et al.Methods for isolation of total RNA to recover miRNAs and other small RNAs from diverse species[J].Methods in Molecular Biology,2010,592:31-50.
[14]郑文岭,马文丽.消化道基因治疗:中医药治疗机制的探讨[J].科技导报,1994,12(12):8-11.
[15]ZHANG L,HOU D,CHEN X,et al.Exogenous plant MIR168a specifically targets mammalian LDLRAP1:evidence of crosskingdom regulation by microRNA[J].Cell Res,2012,22(1):107-126.
[16]YU B,YANG ZY,LI JJ,et al.Methylation as a crucial step in plant microRNA biogenesis[J].Science,2005,307(5711):932-935.
[17]HORWICH MD,LI C,MATRANGA C,et al.The drosophila RNA methyltransferase,Dm Hen1,modifies germline piRNAs and single-stranded siRNAs in RISC[J].Curr Biol,2007,17(14):1265-1272.
[18]SAITO K,SAKAGUCHI Y,SUZUKI T,et al.Pimet,the drosophila homolog of HEN1,mediates 2'-omethylation of piwi-interacting RNAs at their 3'ends[J].Genes Dev,2007,21(13):1603-1608.
[19]CHEN X,ZEN K,ZHANG CY.Reply to lack of detectable oral bioavailability of plant microRNAs after feeding in mice[J].Nat Biotechnol,2013,31(11):967-969.
[20]方杰,胡毅翔,应毕忠,等.米碎花药效微小核糖核酸的鉴定及其靶基因功能分析[J].中草药,2015,46(1):80-85.
[21]王宇亮,王颖芳,杨泽民,等.丹参、生地黄中miRNA在人体血液中分离、鉴定及表达分析[J].中国实验方剂学杂志,2012,18(19):121-124.
[22]ZHOU Z,LI X,LIU J,et al.Honeysuckle-encoded atypical microRNA2911 directly targets influenza A viruses[J].Cell Res,2015,25(1):39-49.
[23]ZHANG T,ZHAO D,WANG Q,et al.MicroRNA-1322 regulates ECRG2 allele specifically and acts as a potential biomarker in patients with esophageal squamous cell carcinoma[J].Mol Carcinog,2013,52(8):581-590.
[24]BOREL F,KONSTANTINOVA P,JANSEN PL.Diagnostic and therapeutic potential of miRNA signatures in patients with hepatocellular carcinoma[J].J Hepatol,2012,56(6):1371-1383.
[25]LU M,ZHANG Q,DENG M,et al.An analysis of human microRNA and disease associations[J].PLo S One,2008,3(10):e3420.
[26]沈朝斌,蔡红,郁兰.黄芪饮片miRNA谱及热力学的稳定性研究[J].中草药,2015,46(20):3079-3085.
[27]向静,黄洁嫦,徐畅,等.甘草水提物中miRNA对人免疫细胞基因表达的影响[J].中国中药杂志,2017,42(9):1752-1756.
[28]CHIN AR,FONG MY,SOMLO G,et al.Cross-kingdom inhibition of breast cancer growth by plant miR159[J].Cell Res,2016,26(2):217.
[29]ZHU K,LIU M,FU Z,et al.Plant microRNAs in larval food regulate honeybee caste development[J].Plos Genetics,2017,13(8):e1006946.
[30]SHAHID S,KIM G,JOHNSON NR,et al.MicroRNAs from the parasitic plant Cuscutacampestris target host messenger RNAs[J].Nature,2018,553(7686):82-85.
[31]吴望军,陈杰,黄瑞华,等.高通量测序技术挖掘猪microRNA的研究进展[J].畜牧兽医学报,2013,44(12):1857-1866.
[32]ARATA H,KOMATSU H,HAN A,et al.Rapid microRNA detection using power-free microfluidic chip:coaxial stacking effect enhances the sandwich hybridization[J].Analyst,2012,137(14):3234-3237.