泰山松花粉多糖体外抗鸡传染性法氏囊病病毒的效果
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摘要
鸡传染性法氏囊超强毒株(vvIBDV)可引起鸡群高死亡率及严重的免疫系统损伤,给养禽业带来巨大的经济损失。为探讨泰山松花粉多糖(TPPPS)体外抗vvIBDV感染鸡胚成纤维细胞(DF-1)作用机制,以DF-1细胞为宿主细胞,利巴韦林(Ribavirin)为抑病毒对照药物,从TPPPS对病毒的直接灭活作用、阻滞作用、抑制吸附及其复制4个方面探讨TPPPS抗vvIBDV机理。结果显示,在无毒质量浓度范围内TPPPS对vvIBDV的最高抑制率为92.4%,Ribavirin的最高抑制率仅为76.7%;在阻滞试验和抑病毒吸附试验中最高抑制率分别达到91.85%和78.36%;在抑制病毒复制试验和直接灭活病毒试验中抑制率很低,表明TPPPS不能抑制病毒复制和直接灭活病毒。结果表明,TPPPS在体外可有效抑制vvIBDV对DF-1细胞的感染,推测其抗病毒机制是TPPPS预处理DF-1细胞从而结合了病毒颗粒在细胞表面的吸附位点及病毒与多糖直接相互作用抑制病毒吸附细胞,或两种作用同时存在。提示TPPPS可作为预防及治疗超强鸡传染性法氏囊病病毒感染的药物。
Very virulent infectious bursal disease virus(vvIBDV)can cause high mortality and severe immunosuppression of chickens and bring huge economic losses to poultry industry.To investigate the antivital mechanism of Taishan Pinus massoniana pollen polysaccharide(TPPPS)on very virulent infectious bursal disease virus infected DF-1 cells,DF-1 cells were used as host cells,ribavirin as an antiviral control drug,the antivital mechanism of TPPPS was discussed from the aspects of TPPPS direct inactivation of virus,blocking effect,inhibition of adsorption and its replication.The result indicated that the inhibition rate of TPPPS against vvIBDV is up to 92.4% within non cytotoxic concentration,ribavirin's highest inhibition rate is only 76.7%.The inhibition rate separately are up to 91.85%and 78.36%in blocking effect and inhibiting adsorption assay.The inhibition rate is very low in the replication inhibition assay and direct inactivation assay,indicating that TPPPS can not inhibit the virus replication and direct inactivation.The study showed that Taishan pine pollen polysaccharide had good anti-vvIBDV activity in vitro,deducing that TPPPS may combine with the viral particles of the adsorption site at the cell surface and TPPPS inhibit virus adsorption cells by interaction,or both effects simultaneously.Suggesting that TPPPS can be used as a drug for the prevention and treatment of infectious bursal disease.
Very virulent infectious bursal disease virus(vvIBDV)can cause high mortality and severe immunosuppression of chickens and bring huge economic losses to poultry industry.To investigate the antivital mechanism of Taishan Pinus massoniana pollen polysaccharide(TPPPS)on very virulent infectious bursal disease virus infected DF-1 cells,DF-1 cells were used as host cells,ribavirin as an antiviral control drug,the antivital mechanism of TPPPS was discussed from the aspects of TPPPS direct inactivation of virus,blocking effect,inhibition of adsorption and its replication.The result indicated that the inhibition rate of TPPPS against vvIBDV is up to 92.4% within non cytotoxic concentration,ribavirin's highest inhibition rate is only 76.7%.The inhibition rate separately are up to 91.85%and 78.36%in blocking effect and inhibiting adsorption assay.The inhibition rate is very low in the replication inhibition assay and direct inactivation assay,indicating that TPPPS can not inhibit the virus replication and direct inactivation.The study showed that Taishan pine pollen polysaccharide had good anti-vvIBDV activity in vitro,deducing that TPPPS may combine with the viral particles of the adsorption site at the cell surface and TPPPS inhibit virus adsorption cells by interaction,or both effects simultaneously.Suggesting that TPPPS can be used as a drug for the prevention and treatment of infectious bursal disease.
引文
[1]张强,李明哲,杨宗让,等.绵马贯众可溶性粉对人工感染鸡传染性法氏囊病的疗效评价[J].中国畜牧兽医,2014,41(11):242-246.
[2]HAFEZ H M,PRUSAS C,RAUE R.Very virulent infectious bursal disease virus(vvIBDV)in vaccinated broiler flock:Course of the disease,identification and characterisation of isolated strain[J].Bmc Res Notes,2003,3(1):1-6.
[3]申海清.抗IBDV中药筛选、作用机制及其临床应用研究.[D].吉林长春:吉林大学,2005.
[4]陈春英,蒋岩,周井炎,等.箬叶多糖及其衍生物对小鼠艾滋病作用的研究[J].中国药理学通报,1999,15(4):336-340.
[5]郑民实,江惟苏,李文,等.牛膝多糖硫酸酯抗Ⅰ型单纯疱疹病毒的实验研究[J].中国医院药学杂志,1996,16(11):483-486.
[6]KALLON S,LI X,JI J,et al.Astragalus polysaccharide enhances immunity and inhibits H9N2avian influenza virus in vitro and in vivo[J].畜牧与兽医,2012,4(s1):325-335.
[7]申利红,王建森,李雅,等.植物多糖的研究及应用进展[J].中国农学通报,2011,27(2):349-352.
[8]魏凯,孙振红,谭燕玲,等.泰山松花粉多糖对小鼠免疫增强作用的研究[J].中国农业科学,2010,43(17):3645-3652.
[9]YU C,WEI K,LIU L,et al.Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity[J].Sci Rep,2017,7:44353.
[10]YANG S,WEI K,JIA F,et al.Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro[J].PloS One,2015,10(3):e0115638.
[11]CHEN M Z,XIE H G,YANG L W,et al.In vitro anti-influenza virus activities of sulfated polysaccharide fractions from gracilaria lemaneiformis[J].Virol Sin,2010,25(5):341.
[12]章振华,李林,景小冬,等.鸡传染性法氏囊病免疫复合物疫苗对SPF雏鸡的免疫效果[J].动物医学进展,2015(10):39-43.
[13]CHEN L,RAN M J,SHAN X X,et al.BAFF enhances B-cell-mediated immune response and vaccineprotection against a very virulent IBDV in chickens[J].Vaccine,2009,27(9):1393-1399.
[4]肖琛闻,季权安,赵凤英,等.传染性法氏囊病病毒免疫学研究进展[J].动物医学进展,2011,32(10):94-98.
[15]GAO Y,LIU W,GAO H,et al.Effective inhibition of infectious bursal disease virus replication in vitro by DNA vector-based RNA interference[J].Antiviral Research,2008,79(2):87.
[16]OOI V E,LIU F.Immunomodulation and anti-cancer activity of polysaccharide-protein complexes[J].Current Medicinal Chemistry,2000,7(7):715-729.
[17]张淑杰,康玉凡.天然活性多糖研究进展[J].食品工业科技,2017,38(2):379-382.
[18]EFFERTH T,ROMERO M R,WOLF D G,et al.The Antiviral Activities of Artemisinin and Artesunate[J].Clin Infect Dis,2008,47(6):804.
[19]SCHNEPF N,CORVO J,PORS S L,et al.Antiviral activity of ganciclovir and artesunate towards human cytomegalovirus in astrocytoma cells[J].Antiviral Res,2011,89(2):186-188.
[20]GRAVINA H D,TAFURI N F,SILVA J A,et al.In vitro assessment of the antiviral potential of trans-cinnamic acid,quercetin and morin against equid herpesvirus 1[J].Res Vet Sci,2011,91(3):158-162.
[21]闫静,孙长江,孙良文,等.黄芩苷体外抗传染性法氏囊病病毒作用[J].中国兽医学报,2014,34(5):807-810.
[22]宋美琴,牛丽,白元生,等.甘草酸二钾体外抗鸡传染性法氏囊炎病毒的作用机制[J].中国兽医科学,2012(6):642-646.
[2]HAFEZ H M,PRUSAS C,RAUE R.Very virulent infectious bursal disease virus(vvIBDV)in vaccinated broiler flock:Course of the disease,identification and characterisation of isolated strain[J].Bmc Res Notes,2003,3(1):1-6.
[3]申海清.抗IBDV中药筛选、作用机制及其临床应用研究.[D].吉林长春:吉林大学,2005.
[4]陈春英,蒋岩,周井炎,等.箬叶多糖及其衍生物对小鼠艾滋病作用的研究[J].中国药理学通报,1999,15(4):336-340.
[5]郑民实,江惟苏,李文,等.牛膝多糖硫酸酯抗Ⅰ型单纯疱疹病毒的实验研究[J].中国医院药学杂志,1996,16(11):483-486.
[6]KALLON S,LI X,JI J,et al.Astragalus polysaccharide enhances immunity and inhibits H9N2avian influenza virus in vitro and in vivo[J].畜牧与兽医,2012,4(s1):325-335.
[7]申利红,王建森,李雅,等.植物多糖的研究及应用进展[J].中国农学通报,2011,27(2):349-352.
[8]魏凯,孙振红,谭燕玲,等.泰山松花粉多糖对小鼠免疫增强作用的研究[J].中国农业科学,2010,43(17):3645-3652.
[9]YU C,WEI K,LIU L,et al.Taishan Pinus massoniana pollen polysaccharide inhibits subgroup J avian leucosis virus infection by directly blocking virus infection and improving immunity[J].Sci Rep,2017,7:44353.
[10]YANG S,WEI K,JIA F,et al.Characterization and biological activity of Taishan Pinus massoniana pollen polysaccharide in vitro[J].PloS One,2015,10(3):e0115638.
[11]CHEN M Z,XIE H G,YANG L W,et al.In vitro anti-influenza virus activities of sulfated polysaccharide fractions from gracilaria lemaneiformis[J].Virol Sin,2010,25(5):341.
[12]章振华,李林,景小冬,等.鸡传染性法氏囊病免疫复合物疫苗对SPF雏鸡的免疫效果[J].动物医学进展,2015(10):39-43.
[13]CHEN L,RAN M J,SHAN X X,et al.BAFF enhances B-cell-mediated immune response and vaccineprotection against a very virulent IBDV in chickens[J].Vaccine,2009,27(9):1393-1399.
[4]肖琛闻,季权安,赵凤英,等.传染性法氏囊病病毒免疫学研究进展[J].动物医学进展,2011,32(10):94-98.
[15]GAO Y,LIU W,GAO H,et al.Effective inhibition of infectious bursal disease virus replication in vitro by DNA vector-based RNA interference[J].Antiviral Research,2008,79(2):87.
[16]OOI V E,LIU F.Immunomodulation and anti-cancer activity of polysaccharide-protein complexes[J].Current Medicinal Chemistry,2000,7(7):715-729.
[17]张淑杰,康玉凡.天然活性多糖研究进展[J].食品工业科技,2017,38(2):379-382.
[18]EFFERTH T,ROMERO M R,WOLF D G,et al.The Antiviral Activities of Artemisinin and Artesunate[J].Clin Infect Dis,2008,47(6):804.
[19]SCHNEPF N,CORVO J,PORS S L,et al.Antiviral activity of ganciclovir and artesunate towards human cytomegalovirus in astrocytoma cells[J].Antiviral Res,2011,89(2):186-188.
[20]GRAVINA H D,TAFURI N F,SILVA J A,et al.In vitro assessment of the antiviral potential of trans-cinnamic acid,quercetin and morin against equid herpesvirus 1[J].Res Vet Sci,2011,91(3):158-162.
[21]闫静,孙长江,孙良文,等.黄芩苷体外抗传染性法氏囊病病毒作用[J].中国兽医学报,2014,34(5):807-810.
[22]宋美琴,牛丽,白元生,等.甘草酸二钾体外抗鸡传染性法氏囊炎病毒的作用机制[J].中国兽医科学,2012(6):642-646.