囊型包虫病患者Th9细胞相关因子mRNA表达特点研究
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摘要
目的通过检测囊型包虫病患者外周血中白细胞介素(IL)-9、嘌呤盒1(PU.1)和干扰素调节因子-4(IRF-4)和IL-4mRNA表达水平,初步探讨Th9细胞相关因子在细粒棘球蚴感染后机体免疫应答中所起的可能作用。方法收集已确诊为囊型包虫病未接受治疗的患者33例作为CE组、经过外科手术和阿苯达唑药物治疗成功的33例CE患者作为治疗后组(PCE组)、30例年龄和性别与患者匹配的健康对照组作为HC组,应用实时荧光定量PCR技术检测各组外周血中IL-9、PU.1、IRF-4和IL-4mRNA表达水平,并进行统计学分析。结果 CE组PU.1、IL-9的表达水平明显高于HC组,差异均有统计学意义(P<0.05)。PU.1在CE组的表达水平明显高于PCE组,差异有统计学意义(P<0.05),IL-9在CE组的表达水平稍高于PCE组,差异无统计学意义(P>0.05)。同时,PU.1、IL-9在PCE组和HC组的表达差异无统计学意义(P>0.05);IRF-4在CE组和PCE组的表达较HC组稍高,而且IRF-4在CE组、HC组、PCE组的表达水平比较,差异无统计学意义(P>0.05);IL-4在CE组的表达水平明显高于HC组和PCE组,差异有统计学意义(P<0.05)。IL-9与PU.1、IL-4之间存在明显的正相关(P<0.05),与IRF-4之间无相关性(P>0.05)。结论 Th9细胞相关因子IL-9、PU.1、IL-4可能参与了细粒棘球蚴感染过程中的免疫应答和调控机制。
Objective To investigate the possible role of Th9 cell-related factors in immune response after echinococcosis granulosus infection by detecting the expression levels of interleukin(IL)-9,purine box 1(PU.1),interferon regulatory factor-4(IRF-4)and IL-4 mRNA in peripheral blood of patients with cystic echinococcosis.Methods 33 patients diagnosed as cystic echinococcosis who did not receive treatment were collected as CE group,33 CE patients who were successfully treated with surgical operation and albendazole drugs as post-treatment group(PCE group),and 30 healthy control groups matched with patients in age and sex as HC group.The expression levels of IL-9,PU.1,IRF-4 and IL-4 mRNA in peripheral blood of each group were detected by real-time fluorescence quantitative PCR technology and statistically analyzed.Results The expression levels of PU.1 and IL-9 in CE group were significantly higher than those in HC group(P<0.05).The expression level of PU.1 in CE group was significantly higher than that in PCE group(P<0.05).The expression level of IL-9 in CE group was slightly higher than that in PCE group,the difference was not statistically significant(P>0.05).At the same time,there was no significant difference in the expression of PU.1 and IL-9 between PCE group and HC group(P>0.05).The expression of IRF-4 in CE group and PCE group was slightly higher than that of HC group,and IRF-4 There was no significant difference in the expression levels of CE group,HC group and PCE group(P>0.05).The expression level of IL-4 in CE group was significantly higher than that in HC group and PCE group.The difference was statistically significant(P<0.05).There was a significant positive correlation between IL-9 and PU.1,IL-4(P<0.05),and no correlation with IRF-4(P>0.05).Conclusion Th9 cell-associated factors IL-9,PU.1 and IL-4 may be involved in the immune response and regulation of Echinococcus granulosus infection.
Objective To investigate the possible role of Th9 cell-related factors in immune response after echinococcosis granulosus infection by detecting the expression levels of interleukin(IL)-9,purine box 1(PU.1),interferon regulatory factor-4(IRF-4)and IL-4 mRNA in peripheral blood of patients with cystic echinococcosis.Methods 33 patients diagnosed as cystic echinococcosis who did not receive treatment were collected as CE group,33 CE patients who were successfully treated with surgical operation and albendazole drugs as post-treatment group(PCE group),and 30 healthy control groups matched with patients in age and sex as HC group.The expression levels of IL-9,PU.1,IRF-4 and IL-4 mRNA in peripheral blood of each group were detected by real-time fluorescence quantitative PCR technology and statistically analyzed.Results The expression levels of PU.1 and IL-9 in CE group were significantly higher than those in HC group(P<0.05).The expression level of PU.1 in CE group was significantly higher than that in PCE group(P<0.05).The expression level of IL-9 in CE group was slightly higher than that in PCE group,the difference was not statistically significant(P>0.05).At the same time,there was no significant difference in the expression of PU.1 and IL-9 between PCE group and HC group(P>0.05).The expression of IRF-4 in CE group and PCE group was slightly higher than that of HC group,and IRF-4 There was no significant difference in the expression levels of CE group,HC group and PCE group(P>0.05).The expression level of IL-4 in CE group was significantly higher than that in HC group and PCE group.The difference was statistically significant(P<0.05).There was a significant positive correlation between IL-9 and PU.1,IL-4(P<0.05),and no correlation with IRF-4(P>0.05).Conclusion Th9 cell-associated factors IL-9,PU.1 and IL-4 may be involved in the immune response and regulation of Echinococcus granulosus infection.
引文
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[10]赵慧,庞楠楠,马海梅,等.泡球蚴感染小鼠Tregs与Th17细胞相关细胞因子的平衡变化[J].中国病原微生物学杂志,2012,2(7):129-131.
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[13]SHIK D,TOMAR S,LEE JB,et al.IL-9-producing cells in the development of IgE-mediated food allergy[J].Semin Immunopathol,2017,39(1):69-77.
[14]徐庆雷,朱宝林,王洪建,等.支气管哮喘患者外周血单个核细胞中IL-9mRNA检测及临床意义[J].国际检验医学杂志,2010,31(12):1345-1346.
[15]NEURATH MF,FINOTTO S.IL-9signaling as key driver of chronic inflammation in mucosal immunity[J].Cytokine Growth Factor Rev,2016(29):93-99.
[16]李志,杨婷婷,李文哲,等.支气管哮喘患儿外周血Th17和Th9细胞及其细胞因子的表达及临床意义研究[J].国际检验医学杂志,2017,38(23):3262-3264.
[17]NEURATH MF,KAPLAN MH.Th9cells in immunity and immunopathological diseases[J].Semin Immunopathol,2017,39(1):1-4.
[18]李艳华,马秀敏,魏琴,等.棘球蚴病患者血清中sPD-1与sPD-L1与相关细胞因子的水平变化[J].细胞与分子免疫学杂志,2014,30(4):421-424.
[19]PANG N,ZHANG F,MA X,et al.Th9/IL-9profile in human echinococcosis:their involvement in immune response during infection by Echinococcusgranuosus[J].Immunological,2014,60(1):49-52.
[20]庞楠楠,刘朝华,张峰波,等.Th9细胞和白介素-9在细粒棘球蚴感染患者中的变化特点及意义[J].免疫学杂志,2014,30(1):49-52.
[21]SCHMITT E,KLEIN M,BOPP T.Th9cells,new players in adaptiveimmunity[J].Trends Immunol,2014,35(2):61-68.
[2]VELDHOEN M,UYTTENHOVE C,SNICK VJ,et al.Transforming growth factorbeta′reprograms′the differentiation of T helper 2cells and promotes an interleukin9producing subset[J].Nat immunol,2008,9(12):1341-1346.
[3]DARDALHON V,AWASTHI A,KWON H,et al.IL-4inhibits TGFbetainduced Foxp3+T cells and,together with TGFbeta,generates IL-9+IL-10+Foxp3(-)effector T cells[J].Nat Immunol,2008,9(12):1347-1355.
[4]CICCIA F,GUGGINO G,FERRANTE A,et al.Interleukin-9 and T helper type 9 cells in rheumatic diseases[J].Clin Exp Immunol,2016,3(30):125-132.
[5]STAUDT V,BOTHUR E,KLEIN M,et al.Interferonregulatory factor 4isessential for The development program of T helper 9cells[J].Immunity,2010,33(2):192-202.
[6]STASSENM,SCHMITTE,BOPPT.From in terleukin-9 to T helper 9 cells[J].Ann N Y AcadSci,2012,47(1):56-58.
[7]WONG M T,YE J J,ALONSO M N,et al.Regulation of human Th9 differentiation by type I interferons and IL-21[J].Immunol Cell Biol,2010,88(6):624-631.
[8]辛燕,张峰波,李艳华,等.棘球蚴病患者血清中可溶性TIM-3/Galactin9的变化[J].免疫学杂志,2014,30(10):910-913.
[9]TUXUN T,APAER S,MA HZ,et al.The potential roel of th9 cell related cytokine and transcripyion factors in patients with hepatic alveolar echinococcosis[J].J Immunol Res,2015,10(5):895416.
[10]赵慧,庞楠楠,马海梅,等.泡球蚴感染小鼠Tregs与Th17细胞相关细胞因子的平衡变化[J].中国病原微生物学杂志,2012,2(7):129-131.
[11]Pang N,Zhang F,Ma X,et al.TGF-β/Smad signaling pathway regulates Th17/Treg balance during Echinococcus multilocularis infection[J].Int Immunopharmacol,2014,20(3),248-267.
[12]赵慧,张峰波,庞楠楠,等.Tim-3/Galectin-9与Th1/Th2相关因子在人细粒棘球蚴感染中的表达研究[J].新疆医科大学学报,2016,39(3):312-315.
[13]SHIK D,TOMAR S,LEE JB,et al.IL-9-producing cells in the development of IgE-mediated food allergy[J].Semin Immunopathol,2017,39(1):69-77.
[14]徐庆雷,朱宝林,王洪建,等.支气管哮喘患者外周血单个核细胞中IL-9mRNA检测及临床意义[J].国际检验医学杂志,2010,31(12):1345-1346.
[15]NEURATH MF,FINOTTO S.IL-9signaling as key driver of chronic inflammation in mucosal immunity[J].Cytokine Growth Factor Rev,2016(29):93-99.
[16]李志,杨婷婷,李文哲,等.支气管哮喘患儿外周血Th17和Th9细胞及其细胞因子的表达及临床意义研究[J].国际检验医学杂志,2017,38(23):3262-3264.
[17]NEURATH MF,KAPLAN MH.Th9cells in immunity and immunopathological diseases[J].Semin Immunopathol,2017,39(1):1-4.
[18]李艳华,马秀敏,魏琴,等.棘球蚴病患者血清中sPD-1与sPD-L1与相关细胞因子的水平变化[J].细胞与分子免疫学杂志,2014,30(4):421-424.
[19]PANG N,ZHANG F,MA X,et al.Th9/IL-9profile in human echinococcosis:their involvement in immune response during infection by Echinococcusgranuosus[J].Immunological,2014,60(1):49-52.
[20]庞楠楠,刘朝华,张峰波,等.Th9细胞和白介素-9在细粒棘球蚴感染患者中的变化特点及意义[J].免疫学杂志,2014,30(1):49-52.
[21]SCHMITT E,KLEIN M,BOPP T.Th9cells,new players in adaptiveimmunity[J].Trends Immunol,2014,35(2):61-68.