养阴舒肝颗粒对肝郁证模型小鼠行为学及脑内单胺类神经递质的影响
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摘要
目的探讨养阴舒肝颗粒对肝郁证模型小鼠行为学和脑内单胺类神经递质的影响。方法采用孤养和慢性不可预见性温和刺激法制备肝郁证模型,观察小鼠造模期间的一般情况、体质量变化,取造模成功小鼠72只,按照体质量随机分为模型组,养阴舒肝颗粒低、中、高剂量组(3、6、12 g·kg~(-1)),逍遥丸组及盐酸氟西汀组,另取12只正常小鼠作为对照组,连续灌胃28 d。采用悬尾实验观察各组小鼠悬尾不动时间;测定额叶皮质和海马的质量;以ELISA试剂盒测定皮质和海马内多巴胺(DA)、5-羟色胺(5-HT)的含量。结果与正常对照组比较,模型组小鼠悬尾不动时间明显延长,额叶皮质和海马内DA、5-HT含量均明显下降,差异有统计学意义(P<0.05,P<0.01)。与模型组比较,养阴舒肝颗粒高剂量组的小鼠悬尾不动时间明显缩短(P<0.05),小鼠额叶皮质DA含量明显升高(P<0.05);养阴舒肝颗粒中剂量组、高剂量组小鼠额叶皮质5-HT含量以及海马区DA、5-HT含量明显升高(P<0.05)。结论养阴舒肝颗粒可以有效改善模型小鼠的肝郁状态,其机制可能与调节肝郁证小鼠的神经递质,升高额叶皮质及海马内的DA、5-HT有关。
Objective To study the effects of Yangyin Shugan(YYSG) granule on praxeology and monoamineneurotransmitters in mice with liver depression syndrome. Methods The models of liver depression syndrome wereinduced by isolation and chronic unpredictable mild stimulation. Seventy-two ICR mice with liver depressionsyndrome were randomly divided into 6 groups:model group,YYSG low,medium and high dose groups(3,6,12 g·kg~(-1)),Xiaoyao pill group and fluoxetine group,another 12 mice were taken as the normal control group. After28 days of continuous gavage, the time of tail suspension, the weights of frontal cortex and hippocampus, thecontents of dopamine(DA) and 5-hydroxytryptamine(5-HT) in the cortex and hippocampus were observed inmice of every group. Results Compared with the blank control group,the time of tail suspension was significantlyprolonged,and the contents of DA and 5-HT in the frontal cortex and hippocampus decreased significantly in themodel group(P < 0.01,P < 0.05). Compared with the model group,the time of tail suspension in YYSG granulehigh dose group was significantly shorter, and the contents of DA in the frontal cortex and hippocampus weresignificantly higher(P < 0.05);the contents of 5-HT in the frontal cortex,and the contents of 5-HT and DA inthe hippocampus of medium and high dose YYSG groups were significantly higher(P < 0.05). Conclusion YYSG granule could effectively improve the state of liver depression,and its mechanism may be related to the regulation ofneurotransmitters,elevation the contents of DA and 5-HT in the frontal cortex and hippocampus.
Objective To study the effects of Yangyin Shugan(YYSG) granule on praxeology and monoamineneurotransmitters in mice with liver depression syndrome. Methods The models of liver depression syndrome wereinduced by isolation and chronic unpredictable mild stimulation. Seventy-two ICR mice with liver depressionsyndrome were randomly divided into 6 groups:model group,YYSG low,medium and high dose groups(3,6,12 g·kg~(-1)),Xiaoyao pill group and fluoxetine group,another 12 mice were taken as the normal control group. After28 days of continuous gavage, the time of tail suspension, the weights of frontal cortex and hippocampus, thecontents of dopamine(DA) and 5-hydroxytryptamine(5-HT) in the cortex and hippocampus were observed inmice of every group. Results Compared with the blank control group,the time of tail suspension was significantlyprolonged,and the contents of DA and 5-HT in the frontal cortex and hippocampus decreased significantly in themodel group(P < 0.01,P < 0.05). Compared with the model group,the time of tail suspension in YYSG granulehigh dose group was significantly shorter, and the contents of DA in the frontal cortex and hippocampus weresignificantly higher(P < 0.05);the contents of 5-HT in the frontal cortex,and the contents of 5-HT and DA inthe hippocampus of medium and high dose YYSG groups were significantly higher(P < 0.05). Conclusion YYSG granule could effectively improve the state of liver depression,and its mechanism may be related to the regulation ofneurotransmitters,elevation the contents of DA and 5-HT in the frontal cortex and hippocampus.
引文
[1]卢军,陈燕芬,杨翠玉,等.卵巢早衰患者中医体质分类与中医证型相关性研究[J].中国中医药现代远程教育,2018,16(4):57-60.
[2]孙松奇.补肾疏肝法治疗闭经[J].世界最新医学信息文摘,2017,17(88):128.
[3]刘小玉.更年期相关性疾病与中医优势[J].中医药导报,2017,23(2):1-4.
[4]卢艺,朱燕,吴冰玲.从“肝”浅谈不孕证治[J].现代中医药,2016,36(6):77-79.
[5]沈坚华,杨洪伟,杨俊雯,等.不孕症相关因素与中医证型相关性调查研究[J].新中医,2016,48(2):135-137.
[6]曹晓静,王小云.养阴舒肝胶囊对POI患者卵巢功能的影响及机制探讨[J].中国实验方剂学杂志,2017,23(4):188-192.
[7]饶玲铭.王小云教授学术经验总结及滋肾养阴疏肝法治疗月经过少的临床研究[D].广州:广州中医药大学,2016.
[8]曹晓静.养阴舒肝胶囊治疗肾虚肝郁型卵巢功能不全的临床研究[D].广州:广州中医药大学,2015.
[9]陈玲,王小云.养阴舒肝胶囊对绝经过渡期妇女月经失调影响临床研究[J].新中医,2013,45(8):118-120.
[10]陈玲.养阴舒肝胶囊对卵巢功能低下影响的临床研究[D].广州:广州中医药大学,2013.
[11]张晓龙.肝郁证动物模型实验研究评述[J].中医学报,2016,31(3):398-401.
[12]王成龙,王林元,王景霞,等.芍药内酯苷及芍药苷对慢性束缚应激配合孤养肝郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响[J].中华中医药杂志,2017,32(7):3121-3125.
[13]石亮.基于大鼠肝郁证模型优化和疏肝解郁功效的柴胡效-毒关联评价[D].济南:山东中医药大学,2017.
[14]孙晓卉,张量.柴胡药理作用的研究进展[J].中国医药导报,2017,14(10):52-55.
[15]WILLNER P,SCHEEL-KRUGER J,BELZUNG C.The neurobiology of depression and antidepressant action[J].Neurosci Biobehav Rev,2013,37(10):2331-2371.
[16]JOCA S,MOREIRA F,WEGENER G.Atypical neurotransmitters and the neurobiology of depression[J].CNS Neurol Disord Drug Targets,2015,14(8):1001.
[17]PATERSON N,MARKOU A.Animal models and treatments for addiction and depression co-morbidity[J].Neurotox Res,2007,11(1):1-32.
[18]李聪.肝郁证模型大鼠高泌乳素及神经-内分泌-免疫机制和相关方药的作用[D].北京:北京中医药大学,2017.
[19]BORROW A,CAMERON N.Estrogenic mediation of serotonergic and neurotrophic systems:implications for female mood disorders[J].Prog Neuropsychopharmacol Biol Psychiatry,2014,54:13-25.
[2]孙松奇.补肾疏肝法治疗闭经[J].世界最新医学信息文摘,2017,17(88):128.
[3]刘小玉.更年期相关性疾病与中医优势[J].中医药导报,2017,23(2):1-4.
[4]卢艺,朱燕,吴冰玲.从“肝”浅谈不孕证治[J].现代中医药,2016,36(6):77-79.
[5]沈坚华,杨洪伟,杨俊雯,等.不孕症相关因素与中医证型相关性调查研究[J].新中医,2016,48(2):135-137.
[6]曹晓静,王小云.养阴舒肝胶囊对POI患者卵巢功能的影响及机制探讨[J].中国实验方剂学杂志,2017,23(4):188-192.
[7]饶玲铭.王小云教授学术经验总结及滋肾养阴疏肝法治疗月经过少的临床研究[D].广州:广州中医药大学,2016.
[8]曹晓静.养阴舒肝胶囊治疗肾虚肝郁型卵巢功能不全的临床研究[D].广州:广州中医药大学,2015.
[9]陈玲,王小云.养阴舒肝胶囊对绝经过渡期妇女月经失调影响临床研究[J].新中医,2013,45(8):118-120.
[10]陈玲.养阴舒肝胶囊对卵巢功能低下影响的临床研究[D].广州:广州中医药大学,2013.
[11]张晓龙.肝郁证动物模型实验研究评述[J].中医学报,2016,31(3):398-401.
[12]王成龙,王林元,王景霞,等.芍药内酯苷及芍药苷对慢性束缚应激配合孤养肝郁模型大鼠行为学及下丘脑-垂体-肾上腺轴的影响[J].中华中医药杂志,2017,32(7):3121-3125.
[13]石亮.基于大鼠肝郁证模型优化和疏肝解郁功效的柴胡效-毒关联评价[D].济南:山东中医药大学,2017.
[14]孙晓卉,张量.柴胡药理作用的研究进展[J].中国医药导报,2017,14(10):52-55.
[15]WILLNER P,SCHEEL-KRUGER J,BELZUNG C.The neurobiology of depression and antidepressant action[J].Neurosci Biobehav Rev,2013,37(10):2331-2371.
[16]JOCA S,MOREIRA F,WEGENER G.Atypical neurotransmitters and the neurobiology of depression[J].CNS Neurol Disord Drug Targets,2015,14(8):1001.
[17]PATERSON N,MARKOU A.Animal models and treatments for addiction and depression co-morbidity[J].Neurotox Res,2007,11(1):1-32.
[18]李聪.肝郁证模型大鼠高泌乳素及神经-内分泌-免疫机制和相关方药的作用[D].北京:北京中医药大学,2017.
[19]BORROW A,CAMERON N.Estrogenic mediation of serotonergic and neurotrophic systems:implications for female mood disorders[J].Prog Neuropsychopharmacol Biol Psychiatry,2014,54:13-25.