模拟大气污染暴露对小鼠多组织急性损伤及肺组织的氧化应激效应
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摘要
采用自主研发的气动雾化法装置,模拟自主呼吸条件下暴露于污染大气环境的真实状况,探讨大气可吸入颗粒物(空气动力学直径≤10μm)不同暴露时间对小鼠肺、脑、心、肝、肾、睾丸等靶器官组织损伤程度及可吸入颗粒物暴露导致小鼠的氧化应激效应.结果显示,可吸入颗粒物经气动雾化法暴露后导致小鼠肺、肾、肝、心等多组织发生病理性损伤,诱导肺组织发生明显的氧化应激,肺组织可作为可吸入颗粒物毒性机制研究的靶标组织.
A self-developed pneumatic atomization apparatus was used to simulate the real conditions of exposure to polluted atmospheric environment under the condition of autonomic respiration and the effects of inhalable particulate matter(aerodynamic diameter ≤10 μm) on lung, brain, heart and liver of mice were studied. The damage degrees of target organs such as kidney and testis and the oxidative stress effect caused by the inhalable particulate matter exposure in mice were examined. The results showed that inhalable particulates caused injury of the lung, kidney and liver in mice via pneumatic atomization.The pathological injury of heart and other tissues induced obvious oxidative stress in lung tissue, which can be used as the target tissue for the study of toxic mechanism of inhalable particles.
A self-developed pneumatic atomization apparatus was used to simulate the real conditions of exposure to polluted atmospheric environment under the condition of autonomic respiration and the effects of inhalable particulate matter(aerodynamic diameter ≤10 μm) on lung, brain, heart and liver of mice were studied. The damage degrees of target organs such as kidney and testis and the oxidative stress effect caused by the inhalable particulate matter exposure in mice were examined. The results showed that inhalable particulates caused injury of the lung, kidney and liver in mice via pneumatic atomization.The pathological injury of heart and other tissues induced obvious oxidative stress in lung tissue, which can be used as the target tissue for the study of toxic mechanism of inhalable particles.
引文
[1]严俊.兰州城区大气污染与居民呼吸,循环系统疾病日住院人数关系的时间序列研究[D].兰州:兰州大学,2010.
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[19]Guo Lin,Li Ben,Miao Juan-juan,et al.Seasonal variation in air particulate matter(PM10)exposure-induced ischemia-like injuries in the rat brain[J].Chemical Research in Toxicology,2015,28:431-439.
[20]Guo Lin,Zhu Nan,Guo Zhen,et al.Particulate matter(PM10)exposure induces endothelial dysfunction and inflammation in rat brain[J].Journal of Hazardous Materials,2012,213-214(4):28-37.
[21]Jurewicz J,Radwan M,Sobala W,et al.The relationship between exposure to air pollution and sperm disomy[J].Environmental and Molecular Mutagenesis,2015,56:50-59.
[22]Canova C,Minelli C,Dunster C,et al.PM10oxidative properties and asthma and COPD[J].Epidemiology,2014,25:467-468.
[23]Tao Chen,Zhang Ying-mei.Lung damage analyzed by machine vision on tissue sections of mice[J].Archives of Toxicology,2018,92(1):425-439.
[24]Tao Chen,Tang Yue,Zhang Lan,et al.Atomization method for verifying size effects of inhalable particles on lung damage of mice[J].Science of the Total Environment,2017,579(1):1476-1484.
[25]顾双,贾玉巧.可吸入颗粒物(PM10)不同组分与健康效应的关系及其毒性机制的研究进展[J].环境卫生学杂志,2016(6):446-450.
[26]Yitshak S,Kloog M,Liberty I,et al.The association between air pollution exposure and glucose and lipids levels[J].The Journal of Clinical Endocrinology and Metabolism,2016,101:2460-2467.
[2]Weinmayr G,Romeo E,De Sario M,et al.Short-term effects of PM2.5and NO2on respiratory health amongchildren with asthma or asthma-like symptoms:a systematic review and meta-analysis[J].Environmental Health Perspectives,2010,118:449-457.
[3]Zhu Rui-xia,Chen Ya-hong,Wu Shao-wei,et al.The relationship between particulate matter(PM10)and hospitalizations and mortality of chronic obstructive pulmonary disease:meta-analysis[J].Journal of Chronic Obstructive Pulmonary Disease,2013,10:307-315.
[4]Lu Feng,Xu Dong-qun,Cheng Yi-bin,et al.Systematic review and meta-analysis of the adverse health effects of ambient PM2.5and PM10pollution in the Chinese population[J].Environmental Research,2015,136:196-204.
[5]唐靖媛,夏茵茵,唐旭,等.重庆市区不同污染水平地区大气可吸入颗粒物对儿童呼吸系统影响的抽样调查[J].第三军医大学学报,2015,37(21):2195-2198.
[6]田瑜,王金艳,李旭,等.兰州市大气污染对人体呼吸系统的影响[J].兰州大学学报:自然科学版,2017,53(3):415-420.
[7]张月琴,何艾浓,闫春良.可吸入颗粒物对呼吸系统疾病的影响[J].职业与健康,2014,30(24):3583-3586.
[8]杨振华,张月华,张全喜,等.沙尘天气可吸入颗粒物对多种心血管疾病日门诊人数的影响[J].中国环境科学,2015,35(1):277-284.
[9]何芳,黄志军,周玉庆,等.长沙市可吸入颗粒物与脑卒中急诊关系的病例交叉研究[J].现代生物医学进展,2014,14(24):4695-4698.
[10]Riva D R,Magalhes C B,Lopes A A,et al.Low dose of fine particulate-matter(PM2.5)can induce acute oxidative stress,inflammation and pulmonary impairment in healthy mice[J].Inhalation Toxicology,2011,23(5):257-267.
[11]Oh S M,Kim H R,Park Y J,et al.Organic extracts of urban air pollution particulate matter(PM2.5)-induced genotoxicity and oxidative stress in human lung bronchial epithelial cells(BEAS-2B cells)[J].Mutation Research,2011,723(2):142-151.
[12]唐悦,陶晨,张迎梅.气动雾化法吸入PM2.5对小鼠妊娠的影响[J].兰州大学学报:自然科学版,2018,54(4):543-547.
[13]Rizzo A M,Corsetto P A,Farina F,et al.Repeated intratracheal instillation of PM10induces lipid reshaping in lung parenchyma and in extra-pulmonary tissues[J].Plos One,2014,9(9):56-58.
[14]Wichmann H E,Spix C,Tuch T,et al.Daily mortality and fine and ultrafine particles in Erfurt,Germany part I:role of particle number and particle mass[J].Research Report(Health Effects Institute),2000,98:85-86.
[15]North M L,Amatullah H,Khanna N,et al.Augmentation of arginase 1 expression by exposure to air pollution exacerbates the airways hyperresponsiveness in murine models of asthma[J].Respiratory Research,2011,304(7):469-480.
[16]Low R B,Bielory L,Qureshi A I,et al.The relation of stroke admissions to recent weather,airborne allergens,air pollution,seasons,upper respiratory infections,and asthma incidence[J].Stroke,2006,37(4):951-957.
[17]Allen J L,Liu X,Weston D,et al.Developmental exposure to concentrated ambient ultrafine particulate matter air pollution in mice results in persistent and sex-dependent behavioral neurotoxicity and glial activation[J].Toxicol Science,2014,140:160-178.
[18]Allen J L,Conrad K,Oberdorster G,et al.Developmental exposure to concentrated ambient particles and preference for immediate reward in mice[J].Environmental Health Perspectives,2013,121:32-38.
[19]Guo Lin,Li Ben,Miao Juan-juan,et al.Seasonal variation in air particulate matter(PM10)exposure-induced ischemia-like injuries in the rat brain[J].Chemical Research in Toxicology,2015,28:431-439.
[20]Guo Lin,Zhu Nan,Guo Zhen,et al.Particulate matter(PM10)exposure induces endothelial dysfunction and inflammation in rat brain[J].Journal of Hazardous Materials,2012,213-214(4):28-37.
[21]Jurewicz J,Radwan M,Sobala W,et al.The relationship between exposure to air pollution and sperm disomy[J].Environmental and Molecular Mutagenesis,2015,56:50-59.
[22]Canova C,Minelli C,Dunster C,et al.PM10oxidative properties and asthma and COPD[J].Epidemiology,2014,25:467-468.
[23]Tao Chen,Zhang Ying-mei.Lung damage analyzed by machine vision on tissue sections of mice[J].Archives of Toxicology,2018,92(1):425-439.
[24]Tao Chen,Tang Yue,Zhang Lan,et al.Atomization method for verifying size effects of inhalable particles on lung damage of mice[J].Science of the Total Environment,2017,579(1):1476-1484.
[25]顾双,贾玉巧.可吸入颗粒物(PM10)不同组分与健康效应的关系及其毒性机制的研究进展[J].环境卫生学杂志,2016(6):446-450.
[26]Yitshak S,Kloog M,Liberty I,et al.The association between air pollution exposure and glucose and lipids levels[J].The Journal of Clinical Endocrinology and Metabolism,2016,101:2460-2467.