徽州地区菊花的指纹图谱鉴别及其评价研究
|
摘要
目的采用高效液相色谱法建立徽州地区菊花的指纹图谱,鉴别菊花不同品种类型,更好地评价徽州地区贡菊的质量。方法采用Topsil HPLC C18色谱柱(4.6mm×250mm,5μm),乙腈(A)-0.1%磷酸水(B)为流动相梯度洗脱,流速为1.0mL/min,检测波长为325nm,建立20批徽州地区菊花的指纹图谱,采用相似度评价、聚类分析及主成分分析方法对数据进行分析。结果 20批菊花样品之间相似度在0.703～0.989之间,不同品种菊花可分别聚为一类,主成分分析确定3个主成分,方差累积贡献率达82.401%。结论本实验建立的指纹图谱精密度、稳定性、重复性良好,可为徽州地区菊花的内在质量控制与品质评价提供初步的研究基础。
Objective To establish the fingerprint of chrysanthemum in Huizhou area,Anhui province,China by high-performance liquid chromatography(HPLC),to identify the different types of chrysanthemum,and to better evaluate the quality of florists'chrysanthemum in Huizhou.Methods HPLC was performed on a Topsil HPLC C18 column(4.6 mm×250 mm,5μm),with a mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B)for gradient elution,at a flow rate of 1.0 mL/min and a detection wavelength of 325 nm.The fingerprints of 20 batches of chrysanthemum in Huizhou area were established,and similarity evaluation,cluster analysis,and principal component analysis were used for data analysis.Results The similarity between the 20 batches of chrysanthemum samples ranged from 0.703 to0.989.Different varieties of chrysanthemum were clustered into one group.Three principal components were determined by the principal component analysis,and the cumulative contribution rate of variance was82.401%.Conclusion The fingerprints established in this experiment have good precision,stability,and repeatability,which provides a preliminary research basis for intrinsic quality control and quality evaluation of chrysanthemum in Huizhou area.
Objective To establish the fingerprint of chrysanthemum in Huizhou area,Anhui province,China by high-performance liquid chromatography(HPLC),to identify the different types of chrysanthemum,and to better evaluate the quality of florists'chrysanthemum in Huizhou.Methods HPLC was performed on a Topsil HPLC C18 column(4.6 mm×250 mm,5μm),with a mobile phase of acetonitrile(A)-0.1% phosphoric acid solution(B)for gradient elution,at a flow rate of 1.0 mL/min and a detection wavelength of 325 nm.The fingerprints of 20 batches of chrysanthemum in Huizhou area were established,and similarity evaluation,cluster analysis,and principal component analysis were used for data analysis.Results The similarity between the 20 batches of chrysanthemum samples ranged from 0.703 to0.989.Different varieties of chrysanthemum were clustered into one group.Three principal components were determined by the principal component analysis,and the cumulative contribution rate of variance was82.401%.Conclusion The fingerprints established in this experiment have good precision,stability,and repeatability,which provides a preliminary research basis for intrinsic quality control and quality evaluation of chrysanthemum in Huizhou area.
引文
[1]中华人民共和国药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:310-311.
[2]王德胜,黄艳梅,石岩,等.菊花化学成分及药理作用研究进展[J].安徽农业科学,2018,46(23):9-11.
[3]谢占芳,张倩倩,朱凌佳,等.菊花化学成分及药理活性研究进展[J].河南大学学报(医学版),2015,34(4):290-300.
[4]瞿璐,王涛,董勇喆,等.菊花化学成分与药理作用的研究进展[J].药物评价研究,2015,38(1):98-104.
[5]王珊,李友连,苏靖,等.中国药用菊花品种及加工方法变迁的研究[J].中国药学杂志,2017,52(7):539-542.
[6]歙县地方志编纂委员会.歙县志[M].合肥:黄山书社,2012:103-118.
[7]张学武.黄山贡菊生产现状及发展对策[J].现代农业科技,2019(8):87-89.
[8]王德群,张玲.我国药用菊花栽培品种和产地调查[J].皖西学院学报,2018,34(5):77-79,92.
[9]姜文青,张亚中,桂双英.贡菊的化学成分及分析方法研究进展[J].安徽医药,2015,19(2):213-218.
[10]屠万倩,刘晓苗,张留记,等.HPLC法同时测定不同产地不同品种菊花中8种成分的含量[J].中药材,2018,41(1):147-150.
[11]刘汉珍,史亚东,俞浩,等.不同品种菊花中4种黄酮类化合物的含量测定比较研究[J].中药材,2016,39(9):2046-2048.
[12]刘刚.霍山石斛生药学鉴别研究[D].合肥:安徽中医药大学,2018.
[13]姜文青,张亚中,金斌.HPLC法同时测定黄山贡菊和七月菊中的4种化学成分[J].中成药,2015,37(10):2211-2215.
[2]王德胜,黄艳梅,石岩,等.菊花化学成分及药理作用研究进展[J].安徽农业科学,2018,46(23):9-11.
[3]谢占芳,张倩倩,朱凌佳,等.菊花化学成分及药理活性研究进展[J].河南大学学报(医学版),2015,34(4):290-300.
[4]瞿璐,王涛,董勇喆,等.菊花化学成分与药理作用的研究进展[J].药物评价研究,2015,38(1):98-104.
[5]王珊,李友连,苏靖,等.中国药用菊花品种及加工方法变迁的研究[J].中国药学杂志,2017,52(7):539-542.
[6]歙县地方志编纂委员会.歙县志[M].合肥:黄山书社,2012:103-118.
[7]张学武.黄山贡菊生产现状及发展对策[J].现代农业科技,2019(8):87-89.
[8]王德群,张玲.我国药用菊花栽培品种和产地调查[J].皖西学院学报,2018,34(5):77-79,92.
[9]姜文青,张亚中,桂双英.贡菊的化学成分及分析方法研究进展[J].安徽医药,2015,19(2):213-218.
[10]屠万倩,刘晓苗,张留记,等.HPLC法同时测定不同产地不同品种菊花中8种成分的含量[J].中药材,2018,41(1):147-150.
[11]刘汉珍,史亚东,俞浩,等.不同品种菊花中4种黄酮类化合物的含量测定比较研究[J].中药材,2016,39(9):2046-2048.
[12]刘刚.霍山石斛生药学鉴别研究[D].合肥:安徽中医药大学,2018.
[13]姜文青,张亚中,金斌.HPLC法同时测定黄山贡菊和七月菊中的4种化学成分[J].中成药,2015,37(10):2211-2215.