玉米须口服液的研制及其抗疲劳功能评价
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摘要
以玉米须、沙棘、山楂、甘草为原料,通过正交试验确定口服液最佳配方,并对其抗疲劳功能进行评价。结果表明:口服液最佳配方为玉米须提取液55%,沙棘提取液25%,山楂提取液10%,甘草提取液6%,三氯蔗糖0.20 g/kg;有效成分含量:多糖15.01 mg/mL,黄酮0.1 mg/mL;口服液组对比玉米须提取液、沙棘提取液、阳性对照组及蒸馏水的小鼠负重游泳时间延长、肝糖原含量增加、血清尿素含量降低,具有较好的抗疲劳效果。所配口服液风味独特,清爽润滑,口感柔和,抗疲劳功能显著。
With the raw material of the extraction liquid from corn silk,sea buckthorn,hawthorn and liquorice,the processing of oral liquid was studied,the optimal formula was decided by orthogonal experiments and its anti-fatigue function was evaluated. The results showed that the best formula of oral liquid was 55 % corn silk extract,25 % sea buckthorn extract,10 % hawthorn extract,6 % of liquorice extract,0.20 g/kg sucralose,which the content of the active ingredient was polysaccharide 15.01 mg/mL and flavone 0.1 mg/mL. Compared with corn silk extract,sea buckthorn extract,positive control group and distilled water,the oral liquid group had better anti-fatigue effects,such as longer the time of weight-loading swimming,higher liver glycogen content and lower serum urea content,so it had a good anti-fatigue effect. The oral liquid was unique in flavor,refreshing and lubricated,with soft taste and anti-fatigue function was remarkable.
With the raw material of the extraction liquid from corn silk,sea buckthorn,hawthorn and liquorice,the processing of oral liquid was studied,the optimal formula was decided by orthogonal experiments and its anti-fatigue function was evaluated. The results showed that the best formula of oral liquid was 55 % corn silk extract,25 % sea buckthorn extract,10 % hawthorn extract,6 % of liquorice extract,0.20 g/kg sucralose,which the content of the active ingredient was polysaccharide 15.01 mg/mL and flavone 0.1 mg/mL. Compared with corn silk extract,sea buckthorn extract,positive control group and distilled water,the oral liquid group had better anti-fatigue effects,such as longer the time of weight-loading swimming,higher liver glycogen content and lower serum urea content,so it had a good anti-fatigue effect. The oral liquid was unique in flavor,refreshing and lubricated,with soft taste and anti-fatigue function was remarkable.
引文
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[23]周淼.柠檬-甘蔗饮料配方的研究[J].湖北农业科学,2014,53(23):5832-5835
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[26]Xi X,Wei X,Wang Y,et al.Determination of tea polysaccharides in Camellia sinensis by a modified phenol-sulfuric acid method[J].Archives of Biological Sciences,2010,62(3):669-676
[27]李富民,谭杰,聂少平,等.青钱柳总黄酮测定方法研究[J].江西食品工业,2006,21(4):34-37
[2]方敏.玉米须活性成分及其生物活性研究[D].武汉:武汉工业学院,2010
[3]Dong Jianwei,Cai Le,Zhu Xiufang,et al.Antioxidant activities and phenolic compounds of cornhusk,corncob and stigma maydis[J].Journal of the Brazilian Che-mical Society,2014,25(11):1956-1964
[4]张卫华.益肾和络汤治疗早期糖尿病肾病的临床观察[J].新疆中医药,2015,33(4):16-19
[5]赵文竹.玉米须功能因子活性评价及其降血糖机理研究[D].长春:吉林大学,2014
[6]郑瑞霞,杨峻山.中国沙棘果实化学成分的研究[J].中草药,2006,37(8):1154-1155
[7]Zhang W,Zhang X,Zou K,et al.Seabuckthorn berry polysaccharide protects against carbon tetrachloride-induced hepatotoxicity in mice via anti-oxidative and anti-inflammatory activities[J].Food&Function,2017,8(9):3130-3138
[8]崔亮,华二伟,薛洁.山楂对消化系统影响的研究进展[J].新疆中医药,2012,30(1):78-79
[9]王玲,吴军林,吴清平,等.山楂降血脂作用和机理研究进展[J].食品科学,2015,36(15):245-248
[10]Yang B,Liu P.Composition and health effects of phenolic compounds in hawthorn(Crataegus spp.)of different origins[J].Journal of the Science of Food&Agriculture,2012,92(8):1578-1590
[11]吕玲霞,辛立红,管仁伟,等.山楂多糖药理作用和提取工艺研究进展[J].现代药物与临床,2016(6):1081-1085
[12]庄步辉,张蕾.浅谈甘草的功效与用法[J].内蒙古中医药,2011,30(22):41
[13]Ji S,Wang Y,Su Z,et al.Ionic liquids-ultrasound based efficient extraction of flavonoid glycosides and triterpenoid saponins from licorice[J].RSC Advances,2018,8(25):13989-13996
[14]周鸿立,刘洋,张扬,等.一种玉米须抗疲劳口服液及其制备方法:CN 104172388 A[P].2014-12-03
[15]南星梅,顾健,谭睿,等.HPLC法同时测定藏药五味沙棘口服液中多指标成分含量[J].中药材,2015,38(3):615-617
[16]徐宁宁.正交试验法优化胃舒口服液中山楂的提取工艺研究[J].江苏理工学院学报,2015(2):60-64
[17]李英杰.复方甘草口服溶液中甘草酸和愈创甘油醚的含量考察[J].药物分析杂志,2012(3):541-544
[18]韩丽,魏春燕,魏博,等.探讨中药保健食品研发的思路[J].食品安全导刊,2015(9):62
[19]周鸿立,张扬,孙佳佳,等.玉米须饮料的研制及其抗氧化活性研究[J].河南工业大学学报(自然科学版),2016,37(3):61-64
[20]宋翔,李悦,魏文静,等.沙棘复合果蔬汁饮料配方研究[J].食品研究与开发,2015(8):43-45
[21]余永婷,王慕欣,谢琼,等.黑枸杞山楂银耳复合饮料工艺的研究[J].食品研究与开发,2018(7):107-113
[22]孙芝杨.淮山药荷叶复合饮料配方的研究[J].食品研究与开发,2017,38(4):71-75
[23]周淼.柠檬-甘蔗饮料配方的研究[J].湖北农业科学,2014,53(23):5832-5835
[24]Zu Y,Sun W,Zhao X,et al.Preparation and characterization of amorphous amphotericin B nanoparticles for oral administration through liquid antisolvent precipitation.[J].European Journal of Pharmaceutical Sciences,2014,53(1):109-117
[25]刘健影,王大为.正交试验优化银耳乳饮料配方[J].乳业科学与技术,2014,37(2):9-13
[26]Xi X,Wei X,Wang Y,et al.Determination of tea polysaccharides in Camellia sinensis by a modified phenol-sulfuric acid method[J].Archives of Biological Sciences,2010,62(3):669-676
[27]李富民,谭杰,聂少平,等.青钱柳总黄酮测定方法研究[J].江西食品工业,2006,21(4):34-37