沉水植物穗花狐尾藻耐盐性与生长
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摘要
通过室内人工海水培养和杭州湾栽培实验,探究了穗花狐尾藻的耐盐能力及在低盐度水域中的生长情况。室内培养表明:穗花狐尾藻断枝可在盐度12以内的人工海水中正常生长;低盐度处理可促进不定根的生长;水体盐度达到15后,穗花狐尾藻叶绿素含量和最大光合效率Fv/Fm降低,生长停滞,大部分植株逐渐死亡。野外栽培表明:从2017年6月到2018年5月,穗花狐尾藻可在杭州湾水体盐度6.06～9.03,水温3.85～33.37℃条件存活,8—10月是穗花狐尾藻的快速生长期;穗花狐尾藻在杭州湾低盐度水体中可进行有性繁殖和无性繁殖,以无性繁殖为主。研究结果为穗花狐尾藻在低盐度水体中建立种群提供了依据。
Salt tolerance and growth properties of Myriophyllum spicatum were investigated by both artificial seawater culture and cultivation in Hangzhou Bay. Results from the indoor cultivation showed that the branch of M. spicatum could grow normally in artificial seawater with salinity less than 12. Low salinity treatment promoted the growth of adventitious roots. When salinity reached 15,the chlorophyll content and maximum photosynthetic efficiency( Fv/Fm) of M. spicatum decreased,the growth was stagnant,and most plants died gradually. Results from field cultivation showed that M. spicatum could survive in the salinity of 6.06-9.03 in Hangzhou Bay from June 2017 to May 2018,with water temperature of 3.85-33.37 ℃. The rapid growth period of M. spicatum was from August to October. M. spicatum can carry on sexual reproduction and vegetative reproduction in the low salinity water of Hangzhou Bay,mainly by vegetative reproduction. These results provide basis for the establishment of M. spicatum in waters with low salinity.
Salt tolerance and growth properties of Myriophyllum spicatum were investigated by both artificial seawater culture and cultivation in Hangzhou Bay. Results from the indoor cultivation showed that the branch of M. spicatum could grow normally in artificial seawater with salinity less than 12. Low salinity treatment promoted the growth of adventitious roots. When salinity reached 15,the chlorophyll content and maximum photosynthetic efficiency( Fv/Fm) of M. spicatum decreased,the growth was stagnant,and most plants died gradually. Results from field cultivation showed that M. spicatum could survive in the salinity of 6.06-9.03 in Hangzhou Bay from June 2017 to May 2018,with water temperature of 3.85-33.37 ℃. The rapid growth period of M. spicatum was from August to October. M. spicatum can carry on sexual reproduction and vegetative reproduction in the low salinity water of Hangzhou Bay,mainly by vegetative reproduction. These results provide basis for the establishment of M. spicatum in waters with low salinity.
引文
范国兰,李伟.2005.穗花狐尾藻(Myriophyllum spicatum L.)在不同程度富营养化水体中的营养积累特点及营养分配对策.植物科学学报,23(3):267-271.
付蓉.2016.玉米自交系盐胁迫响应miRNA及其靶基因的鉴定研究(硕士学位论文).南通:南通大学.
客涵.2014.南四湖沉水、浮叶植物群落结构与水环境因子相关研究(硕士学位论文).济南:山东大学.
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刘弘,马杰,李新峥,等.2012.不同营养液配方及其浓度对椒草的影响.贵州农业科学,40(12):44-46.
马剑敏,马顷,苏秀燕,等.2011.水绵对3种沉水植物的化感作用研究.河南农业科学,40(9):70-73.
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王祎,宋光丽,杨万年,等.2007.光周期对穗花狐尾藻生长、开花与种子形成的影响.水生生物学报,31(1):107-111.
温刘君,朴顺姬,易津.2009.4种小麦族牧草种子耐盐补偿生长特性研究.中国草地学报,31(6):30-32.
吴业颖,操瑜,郑志伟,等.2017.穗花狐尾藻在不同营养水平湖泊繁殖策略的比较研究.水生态学杂志,38(1):30-34.
徐鲜钧,沈宝川,祁建民.2007.植物耐盐性及其生理生化指标的研究进展.亚热带农业研究,3(4):275-280.
许雪,季延海,张广华,等.2015.不同营养液配方对黄瓜营养液育苗效果的影响.北方园艺,(11):44-48.
易文利,王圣瑞,杨苏文,等.2011.有机质腐解对穗花狐尾藻生长及生理的影响.中国环境科学,31(10):1718-1724.
张俊叶,张力君,赵青山,等.2012.几种豆科牧草种子萌发期的耐盐性.中国草地学报,34(4):116-120.
赵风斌,王丽卿,季高华,等.2012.盐胁迫对3种沉水植物生物学指标及叶片中丙二醛含量的影响.环境污染与防治,(10):40-44.
周洁,王东.2012.狐尾藻属狭域种和广布种断枝的生长与再生能力比较研究.水生生物学报,36(2):316-322.
周璐璐,伏兵哲,许冬梅,等.2015.盐胁迫对沙芦草萌发特性影响及耐盐性评价.草业科学,32(8):1252-1259.
Brownell PF,Crossland CJ.1972.The requirement for sodium as a micronutrient by species having the C dicarboxylic photosynthetic pathway.Plant Physiology,49:794-797.
Fan XD,Wang JQ,Yang N,et al.2013.Gene expression profiling of soybean leaves and roots under salt,saline-alkali and drought stress by high-throughput Illumina sequencing.Gene,512:392-402.
Irmgard B,Maja B,Melanie H,et al.2008.Epiphytic bacterial community composition on two common submerged macrophytes in brackish water and freshwater.BMC Microbiology,8:58.
Jiang Y,Yang B,Harris NS,et al.2007.Comparative proteomic analysis of Na Cl stress-responsive proteins in Arabidopsis roots.Journal of Experimental Botany,58:3591-3607.
Johnson JA,Newman RM.2011.A comparison of two methods for sampling biomass of aquatic plants.Journal of Aquatic Plant Management,49:1-8.
Li F,Zhu L,Xie Y,et al.2015.Colonization by fragments of the submerged macrophyte Myriophyllum spicatum under different sediment type and density conditions.Scientific Reports,5:11821.
Li F,Zhu L,Xie Y,et al.2016.Fragment growth performance of the invasive submerged macrophyte Myriophyllum spicatum under conditions of different water depths and sediment types.Aquatic Ecology,50:727-734.
Liu Q,Sun B,Huo Y,et al.2018.Nutrient bioextraction and microalgae growth inhibition using submerged macrophyte Myriophyllum spicatum in a low salinity area of East China Sea.Marine Pollution Bulletin,127:67-72.
Peng Z,Wang M,Li F,et al.2009.A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat.Molecular&Cellular Proteomics,8:2676-2686.
Rengasamy P.2006.World salinization with emphasis on Australia.Journal of Experimental Botany,57:1017-1023.
Rodrigo MA,Rojo C,Alonso-Guillén JL,et al.2013.Restoration of two small Mediterranean lagoons:The dynamics of submerged macrophytes and factors that affect the success of revegetation.Ecological Engineering,54:1-15.
Umetsu CA,Thomaz SM.2012.The colonization,regeneration,and growth rates of macrophytes from fragments:A comparison between exotic and native submerged aquatic species.Aquatic Ecology,46:443-449.
Wu Z,Yu D,Wang Z,et al.2015.Great influence of geographic isolation on the genetic differentiation of Myriophyllum spicatum under a steep environmental gradient.Scientific Reports,5:15618.
Xie D,Yu D.2011.Size-related auto-fragment production and carbohydrate storage in auto-fragment of Myriophyllum spicatum L.in response to sediment nutrient and plant density.Hydrobiologia,658:221-231.
Yao D,Zhang X,Zhao X,et al.2011.Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton(Gossypium hirsutum L.).Genomics,98:47-55.
Zhang L,Tian LH,Zhao JF,et al.2009.Identification of an apoplastic protein involved in thei Initial phase of salt stress response in rice root by two-dimensional electrophoresis.Plant Signaling&Behavior,149:916-928.
Zrb C,Herbst R,Forreiter C,et al.2010.Short-term effects of salt exposure on the maize chloroplast protein pattern.Proteomics,9:4209-4220.
付蓉.2016.玉米自交系盐胁迫响应miRNA及其靶基因的鉴定研究(硕士学位论文).南通:南通大学.
客涵.2014.南四湖沉水、浮叶植物群落结构与水环境因子相关研究(硕士学位论文).济南:山东大学.
雷泽湘,徐德兰,黄沛生,等.2006.太湖沉水和浮叶植被及其水环境效应研究.生态环境学报,15(2):239-243.
刘弘,马杰,李新峥,等.2012.不同营养液配方及其浓度对椒草的影响.贵州农业科学,40(12):44-46.
马剑敏,马顷,苏秀燕,等.2011.水绵对3种沉水植物的化感作用研究.河南农业科学,40(9):70-73.
马剑敏,严国安,罗岳平,等.1997.武汉东湖受控生态系统中水生植被恢复结构优化及水质动态.湖泊科学,9(4):359-363.
苏倩,刘存歧,杨军,等.2012.小西淀穗花狐尾藻的生长特性与氮磷吸收规律研究.水生态学杂志,33(6):50-55.
王祎,宋光丽,杨万年,等.2007.光周期对穗花狐尾藻生长、开花与种子形成的影响.水生生物学报,31(1):107-111.
温刘君,朴顺姬,易津.2009.4种小麦族牧草种子耐盐补偿生长特性研究.中国草地学报,31(6):30-32.
吴业颖,操瑜,郑志伟,等.2017.穗花狐尾藻在不同营养水平湖泊繁殖策略的比较研究.水生态学杂志,38(1):30-34.
徐鲜钧,沈宝川,祁建民.2007.植物耐盐性及其生理生化指标的研究进展.亚热带农业研究,3(4):275-280.
许雪,季延海,张广华,等.2015.不同营养液配方对黄瓜营养液育苗效果的影响.北方园艺,(11):44-48.
易文利,王圣瑞,杨苏文,等.2011.有机质腐解对穗花狐尾藻生长及生理的影响.中国环境科学,31(10):1718-1724.
张俊叶,张力君,赵青山,等.2012.几种豆科牧草种子萌发期的耐盐性.中国草地学报,34(4):116-120.
赵风斌,王丽卿,季高华,等.2012.盐胁迫对3种沉水植物生物学指标及叶片中丙二醛含量的影响.环境污染与防治,(10):40-44.
周洁,王东.2012.狐尾藻属狭域种和广布种断枝的生长与再生能力比较研究.水生生物学报,36(2):316-322.
周璐璐,伏兵哲,许冬梅,等.2015.盐胁迫对沙芦草萌发特性影响及耐盐性评价.草业科学,32(8):1252-1259.
Brownell PF,Crossland CJ.1972.The requirement for sodium as a micronutrient by species having the C dicarboxylic photosynthetic pathway.Plant Physiology,49:794-797.
Fan XD,Wang JQ,Yang N,et al.2013.Gene expression profiling of soybean leaves and roots under salt,saline-alkali and drought stress by high-throughput Illumina sequencing.Gene,512:392-402.
Irmgard B,Maja B,Melanie H,et al.2008.Epiphytic bacterial community composition on two common submerged macrophytes in brackish water and freshwater.BMC Microbiology,8:58.
Jiang Y,Yang B,Harris NS,et al.2007.Comparative proteomic analysis of Na Cl stress-responsive proteins in Arabidopsis roots.Journal of Experimental Botany,58:3591-3607.
Johnson JA,Newman RM.2011.A comparison of two methods for sampling biomass of aquatic plants.Journal of Aquatic Plant Management,49:1-8.
Li F,Zhu L,Xie Y,et al.2015.Colonization by fragments of the submerged macrophyte Myriophyllum spicatum under different sediment type and density conditions.Scientific Reports,5:11821.
Li F,Zhu L,Xie Y,et al.2016.Fragment growth performance of the invasive submerged macrophyte Myriophyllum spicatum under conditions of different water depths and sediment types.Aquatic Ecology,50:727-734.
Liu Q,Sun B,Huo Y,et al.2018.Nutrient bioextraction and microalgae growth inhibition using submerged macrophyte Myriophyllum spicatum in a low salinity area of East China Sea.Marine Pollution Bulletin,127:67-72.
Peng Z,Wang M,Li F,et al.2009.A proteomic study of the response to salinity and drought stress in an introgression strain of bread wheat.Molecular&Cellular Proteomics,8:2676-2686.
Rengasamy P.2006.World salinization with emphasis on Australia.Journal of Experimental Botany,57:1017-1023.
Rodrigo MA,Rojo C,Alonso-Guillén JL,et al.2013.Restoration of two small Mediterranean lagoons:The dynamics of submerged macrophytes and factors that affect the success of revegetation.Ecological Engineering,54:1-15.
Umetsu CA,Thomaz SM.2012.The colonization,regeneration,and growth rates of macrophytes from fragments:A comparison between exotic and native submerged aquatic species.Aquatic Ecology,46:443-449.
Wu Z,Yu D,Wang Z,et al.2015.Great influence of geographic isolation on the genetic differentiation of Myriophyllum spicatum under a steep environmental gradient.Scientific Reports,5:15618.
Xie D,Yu D.2011.Size-related auto-fragment production and carbohydrate storage in auto-fragment of Myriophyllum spicatum L.in response to sediment nutrient and plant density.Hydrobiologia,658:221-231.
Yao D,Zhang X,Zhao X,et al.2011.Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of cotton(Gossypium hirsutum L.).Genomics,98:47-55.
Zhang L,Tian LH,Zhao JF,et al.2009.Identification of an apoplastic protein involved in thei Initial phase of salt stress response in rice root by two-dimensional electrophoresis.Plant Signaling&Behavior,149:916-928.
Zrb C,Herbst R,Forreiter C,et al.2010.Short-term effects of salt exposure on the maize chloroplast protein pattern.Proteomics,9:4209-4220.