地黄多糖超声提取工艺优化及其抗氧化活性
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摘要
目的优化地黄多糖超声提取工艺,并评价其抗氧化活性。方法在单因素试验基础上,以料液比、提取时间、提取温度、醇沉浓度为影响因素,多糖得率为评价指标,正交试验优化超声提取工艺。然后,考察分步醇沉(50%、70%、80%、90%乙醇,相应部位分别命名为RGPS50、RGPS70、RGPS80、RGPS90)对6个品种中多糖含有量的影响,以及多糖对DPPH自由基的清除作用。结果最佳条件为料液比1∶30,提取时间100 min,提取温度50℃,醇沉浓度90%,多糖得率7. 75%。各品种中多糖含有量依次为85-5>星科>金九>沁怀>北京3号>怀丰。RGPS90中多糖含有量最高,抗氧化活性最弱; RGPS80中多糖含有量相对较低,抗氧化活性最强。结论该方法稳定可靠,可用于超声提取地黄多糖。不同品种、醇沉部位地黄中多糖含有量和抗氧化活性差异明显。
AIM To optimize the ultrasonic extraction process for polysaccharides from Rehmannia glutinosa Libosch.,and to evaluate the antioxidant activity. METHODS With solid-liquid ratio,extraction time,extraction temperature,ethanol precipitation concentration as influencing factors,polysaccharides yield as an evaluation index,orthogonal test was applied to optimizing the ultrasonic extraction process on the basis of single factor test.Subsequently the effects of stepwise ethanol precipitation(ethanol at concentrations of 50%,70%,80%,90%,whose corresponding fractions were named as RGPS50,RGPS70,RGPS80,RGPS90,respectively) on the contents of six polysaccharides were investigated,then the scavenging effect of polysaccharides on DPPH free radical was detected. RESULTS The optimal conditions were determined to be 1 ∶ 30 for solid-liquid ratio,100 min for extraction time,50 ℃ for extraction temperature,and 90% for ethanol precipitation concentration,the polysaccharides yield was 7. 75%. The contents of various polysaccharides were found in sequence of 85-5 > Xingke > Jinjiu > Qinhuai > Beijing No. 3 > Huaifeng. Yet the polysaccharides in RGPS90,with the highest content,displayed the weakest antioxidant activity; while the polysaccharides in RGPS80,with relatively low content,showed the strongest antioxidant activity. CONCLUSION This stable and reliable method can be used for the ultrasonic extraction for polysaccharides from R. glutinosa. Different varieties and ethanol precipitation fractions of R. glutinosa polysaccharides demonstrate obvious differences in contents and antioxidant activities.
AIM To optimize the ultrasonic extraction process for polysaccharides from Rehmannia glutinosa Libosch.,and to evaluate the antioxidant activity. METHODS With solid-liquid ratio,extraction time,extraction temperature,ethanol precipitation concentration as influencing factors,polysaccharides yield as an evaluation index,orthogonal test was applied to optimizing the ultrasonic extraction process on the basis of single factor test.Subsequently the effects of stepwise ethanol precipitation(ethanol at concentrations of 50%,70%,80%,90%,whose corresponding fractions were named as RGPS50,RGPS70,RGPS80,RGPS90,respectively) on the contents of six polysaccharides were investigated,then the scavenging effect of polysaccharides on DPPH free radical was detected. RESULTS The optimal conditions were determined to be 1 ∶ 30 for solid-liquid ratio,100 min for extraction time,50 ℃ for extraction temperature,and 90% for ethanol precipitation concentration,the polysaccharides yield was 7. 75%. The contents of various polysaccharides were found in sequence of 85-5 > Xingke > Jinjiu > Qinhuai > Beijing No. 3 > Huaifeng. Yet the polysaccharides in RGPS90,with the highest content,displayed the weakest antioxidant activity; while the polysaccharides in RGPS80,with relatively low content,showed the strongest antioxidant activity. CONCLUSION This stable and reliable method can be used for the ultrasonic extraction for polysaccharides from R. glutinosa. Different varieties and ethanol precipitation fractions of R. glutinosa polysaccharides demonstrate obvious differences in contents and antioxidant activities.
引文
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[12]于雷,李晓坤,杨云,等.墨旱莲多糖的过氧化氢脱色方法研究[J].辽宁中医杂志, 2012, 39(9):1832-1834.
[13]程旺开,许月明,张冬冬.响应面优化黄秋葵叶多糖的提取工艺及其抗氧化活性考察[J].中国实验方剂学杂志,2017,23(4):38-42.
[14]熊磊,陈慧,胡文兵,等.黄金茶多糖超声提取工艺及体外抗氧化研究[J].江西农业大学学报,2017,39(4):801-809.
[15]董红敏,李素清,牛小勇,等.正交实验优化川明参多糖超声提取工艺[J].食品工业科技,2014,35(8):306-309,322.
[16]林洵,陈建福.小藜多糖的提取工艺研究[J].乐山师范学院学报,2016,31(8):18-23.
[17]胡伟莲,戴德慧.红参药渣中多糖的提取及其醇沉性质分析[J].食品研究与开发,2015,36(4):58-61.
[2] Tan W,Yu K Q,Liu Y Y,et al. Anti-fatigue activity of polysaccharides extract from Radix Rehmanniae Preparata[J]. Int J Biol Macromol,2012,50(1):59-62.
[3]王军,于震,李更生,等.地黄苷A对“阴虚”及免疫功能低下小鼠的药理作用[J].中国药学杂志,2002,37(1):20-22.
[4] Cui Y,Rong C L,Wang J M,et al. Mechanism-based antianxiety effects of polysaccharides extracted from Shudihuang(Radix Rehmanniae Preparata)by two-dimensional electrophoresis analysis in rat hippocampus proteins[J]. J Tradit Chin Med,2013,33(4):524-530.
[5]蔡春沉,王洪玺,王肃.地黄多糖对肥胖糖尿病大鼠模型的治疗作用及对血清中GLP-1、GIP水平的影响[J].中国老年学杂志,2013,33(18):4506-4507.
[6]许春鹃,薛进华,范启兰.地黄多糖提取分离技术的研究进展[J].赣南医学院学报,2012,32(2):315-316.
[7]李红霞,许闵,孟江,等.怀地黄多糖的含量测定[J].河南科学,2002,20(2):144-146.
[8]程旺开,许月明,张冬冬.响应面优化黄秋葵叶多糖的提取工艺及其抗氧化活性考察[J].中国实验方剂学杂志,2017,23(4):38-42.
[9]吴诗云,钟益宁,张焱,等.熟地黄多糖含量测定方法研究[J].安徽农业科学,2016,44(35):154-156,161.
[10]秦亚东,汪荣斌,周娟娟.白芍多糖脱色工艺研究[J].中成药,2015,37(12):2783-2787.
[11]李王武,王曦林,吕伟旗,等.不同种源玉竹多糖含量比较及相关性分析[J].中国实验方剂学杂志,2017,23(7):46-51.
[12]于雷,李晓坤,杨云,等.墨旱莲多糖的过氧化氢脱色方法研究[J].辽宁中医杂志, 2012, 39(9):1832-1834.
[13]程旺开,许月明,张冬冬.响应面优化黄秋葵叶多糖的提取工艺及其抗氧化活性考察[J].中国实验方剂学杂志,2017,23(4):38-42.
[14]熊磊,陈慧,胡文兵,等.黄金茶多糖超声提取工艺及体外抗氧化研究[J].江西农业大学学报,2017,39(4):801-809.
[15]董红敏,李素清,牛小勇,等.正交实验优化川明参多糖超声提取工艺[J].食品工业科技,2014,35(8):306-309,322.
[16]林洵,陈建福.小藜多糖的提取工艺研究[J].乐山师范学院学报,2016,31(8):18-23.
[17]胡伟莲,戴德慧.红参药渣中多糖的提取及其醇沉性质分析[J].食品研究与开发,2015,36(4):58-61.