畜禽精液冷冻损伤保护研究进展
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摘要
精液冷冻过程中液体冰晶化造成的机械损伤和氧化自由基造成的氧化损伤是畜禽冷冻精液产业化的主要限制因素。在冻精中添加外源抗氧化剂维持氧化还原平衡状态以此减轻氧化损伤、通过添加抗冻剂和控制冷冻速率来减少冰晶产生和减轻机械损伤成为畜禽冻精技术研究的重点。本文从不同种类抗氧化剂的抗氧化机理及其在冻精中的应用现状、添加抗冻剂和控制冷冻速率等方面减少机械损伤进行综述,以期为畜禽精液冷冻技术提供理论和技术参考。
The oxidative damage caused by mechanical damage and oxidative free radical formation caused by liquid ice crystallization during freezing is the main limiting factor for the industrialization of livestock and poultry frozen semen Adding exogenous antioxidants to the frozen essence to maintain redox equilibrium can reduce oxidative damage.The reduction of ice crystal production and the reduction of mechanical damage by adding antifreeze and controlling the freezing rate have become the focus of research on livestock and frozen technology.In this paper,the anti-oxidation mechanism of different kinds of antioxidants and its application status in frozen sperm,the addition of cryoprotectants and the control of freezing rate are reviewed in order to provide theoretical and technical references for livestock and poultry semen freezing technology.
The oxidative damage caused by mechanical damage and oxidative free radical formation caused by liquid ice crystallization during freezing is the main limiting factor for the industrialization of livestock and poultry frozen semen Adding exogenous antioxidants to the frozen essence to maintain redox equilibrium can reduce oxidative damage.The reduction of ice crystal production and the reduction of mechanical damage by adding antifreeze and controlling the freezing rate have become the focus of research on livestock and frozen technology.In this paper,the anti-oxidation mechanism of different kinds of antioxidants and its application status in frozen sperm,the addition of cryoprotectants and the control of freezing rate are reviewed in order to provide theoretical and technical references for livestock and poultry semen freezing technology.
引文
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[17]Khiabani A B,Moghaddam G,Kia H D.Effects of adding different levels of Glutamine to modified Beltsville extender on the survival of frozen rooster semen[J].Anim Reprod Sci,2017,184:172-177.
[18]柴局.内蒙古绒山羊精液冷冻保存的研究[D].呼和浩特:内蒙古农业大学,2011.
[19]Partyka A,Rodak O,Bajzert J,et al.The Effect of L-carnitine,hypotaurine,and taurine supplementation on the quality of cryopreserved chicken semen[J].Biomed Res Int,2017,2017(3):1-8.
[20]刘亚伟.α-硫辛酸、维生素P和L-肉碱对猪精液常温和低温保存效果的研究[D].杨凌:西北农林科技大学,2014.
[21]谢东淇,刘琦,苏泽智,等.牛磺酸与抗生素复合剂对猪精液常温保存效果的影响[J].西北农业学报,2017,26(6):820-826.
[22]Fattah A,Sharafi M,Masoudi R,et al.L-carnitine in rooster semen cryopreservation:flow cytometric,biochemical and motion findings for frozen-thawed sperm[J].Cryobiology,2017,74:148-153.
[23]李伟,李克,商学军,等.肉毒碱体外对人精子活率及运动参数的影响[J].现代预防医学,2006,33(5):671-674.
[24]白志梅,李青旺,孙秀柱.L-肉碱对猪精液冷冻保存效果的研究[J].畜牧与兽医,2013,45(4):14-17.
[25]Longobardi V,Salzano A,Campanile G,et al.Carnitine supplementation decreases capacitation-likechanges of frozenthawed buffalo spermatozoa[J].Theriogenology,2017,88:236-243.
[26]康孟阳.四种抗氧化剂在关中驴精液冷冻保存中的应用效果研究[D].杨凌:西北农林科技大学,2016.
[27]南向斌.三种多糖和冷冻平衡时间对牛精液冷冻效果影响的研究[D].杨凌:西北农林科技大学,2010.
[28]韩毅冰,邹纯霞,秦鹏春,等.透明质酸对猪精子活率、获能、顶体反应及体外受精穿透率的影响[J].中国兽医学报,1997,17(6):83-88.
[29]权国波,吴国权,吕春荣,等.肌醇类冰晶抑制剂和海藻糖对山羊精子冷冻保存效果的影响[J].家畜生态学报,2017,38(5):亻备48-53.
[30]赵占强,周玉香,洪涛.不同浓度海藻糖对牛冷冻精液品质的影响[J].中国牛业科学,2017,43(1):27-29.
[31]何涛,赵娜.鸡精液稀释液中添加三种多糖对种蛋孵化率的影响[J].湖北畜牧兽医,2018,39(1):5-7.
[32]任子利,宋天增,张健,等.红景天多糖对藏猪精液冻后品质及精子基因组DNA甲基化的影响[J].中国兽医学报,2017,37(7):1385-1388,1393.
[33]刘颂东,王前.透明质酸、BSA和SOD在猪精液液态保存中对精子活力和受胎力的影响[J].畜牧与兽医,2003,35(8):16-17.
[34]张斌,纪康,武彩红,等.稀释液中添加谷胱甘肽和透明质酸对猪精液超低温保存效果的影响[J].畜牧与兽医,2014,46(12):29-33.
[35]Lotfi S,Mehri M,Sharafi M,et al.Hyaluronic acid improves frozen-thawed sperm quality and fertility potential in rooster[J].Anim Reprod Sci,2017,184:204-210.
[36]李崇阳,朱化彬,郝海生,等.抗氧化物质对提高家畜冻精受精能力的研究进展[J].中国畜牧杂志,2017,53(8):21-25.
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