原玻璃节杆菌Y1对磺胺吡啶抗药性及其生长动力学
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摘要
测定了原玻璃节杆菌(Arthrobacter protophormiae)Y1对磺胺吡啶(SPY)的抗药性,比较了无SPY压力不同接种量以及不同浓度SPY压力下该菌在LB培养基中的生长特性,建立了该菌不同条件下的一级生长模型,并比较其最大比生长速率(μmax)、延滞期(λ)及最大生长量(a)。结果表明,SPY对原玻璃节杆菌Y1的最小抑菌浓度为13mg/mL。SGompertz模型能较好地拟合了菌株Y1的生长曲线,拟合度较高,模型的误差较小。无SPY压力不同接种量下菌株Y1最大比生长速率(μmax=0.24830~0.29797)和最大生长量(a=2.03441~2.21512)无显著差异,但是延滞期(λ=6.12695~3.11051)随接种量增大而减小。当存在SPY压力时,最大比生长速率(μmax=0.24225~0.12034)以及延滞期(λ=4.71308~5.91191)均随着SPY浓度的提高而显著降低;当SPY浓度为3mg/mL时,菌株Y1的最大比生长速率(μmax=0.24225)和最大生物量(a=2.12467)与无SPY时相当,但其延滞期稍有降低(λ=4.71308)。
The resistance of Arthrobacter protophormiae Y1 against sulfapyridine(SPY)was determined.The growth characteristics of this strain in LB medium with and without antibiotics were compared under different inoculation doses.The first growth model was established and the maximum specific growth rate(μmax),the lag phage(λ)and the maximum biomass(a)were compared.The results showed that the minimum inhibitory concentration(MIC)of SPY against A.protophormiae Y1 was 13 mg/mL.SGompertz model could better fit the growth of strain Y1 with high fitting degree and small modeling error.No significant difference in the maximum specific growth rate(μmax=0.24830～0.29797)and the maximum biomass(a=2.03441～2.21512)was observed for strain Y1 in the absence of SPY,but the lag phase(λ=6.12695～3.11051)decreased with the increasing of inoculum size.In the presence of SPY,the maximum specific growth rate(μmax=0.24225～0.12034)and lag phase(λ=4.71308～5.91191)were significantly reduced with the increasing of SPY concentrations.The maximum specific growth rate(μmax=0.24225)and the maximum biomass(a=2.12467)of strain Y1 at 3 mg/mL SPY were similar to those without SPY,but with a slight decrease in lag phase(λ=4.71308).The study provided basic information for the engineering application and risk management of A.protophormiae Y1.
The resistance of Arthrobacter protophormiae Y1 against sulfapyridine(SPY)was determined.The growth characteristics of this strain in LB medium with and without antibiotics were compared under different inoculation doses.The first growth model was established and the maximum specific growth rate(μmax),the lag phage(λ)and the maximum biomass(a)were compared.The results showed that the minimum inhibitory concentration(MIC)of SPY against A.protophormiae Y1 was 13 mg/mL.SGompertz model could better fit the growth of strain Y1 with high fitting degree and small modeling error.No significant difference in the maximum specific growth rate(μmax=0.24830～0.29797)and the maximum biomass(a=2.03441～2.21512)was observed for strain Y1 in the absence of SPY,but the lag phase(λ=6.12695～3.11051)decreased with the increasing of inoculum size.In the presence of SPY,the maximum specific growth rate(μmax=0.24225～0.12034)and lag phase(λ=4.71308～5.91191)were significantly reduced with the increasing of SPY concentrations.The maximum specific growth rate(μmax=0.24225)and the maximum biomass(a=2.12467)of strain Y1 at 3 mg/mL SPY were similar to those without SPY,but with a slight decrease in lag phase(λ=4.71308).The study provided basic information for the engineering application and risk management of A.protophormiae Y1.
引文
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[3]王丽梅,罗义,毛大庆,等.抗生素抗性基因在环境中的传播扩散及抗性研究方法[J].应用生态学报,2010,21(4):1063~1069.
[4]周启星,罗义,王美娥.抗生素的环境残留、生态毒性及抗性基因污染[J].生态毒理学报,2007,2(3):243~251.
[5]Heuer H,Solehati Q,Zimmerling U,et al.Accumulation of sulfonamide resistance genes in arable soils due to repeated application of manure containing sulfadiazine[J].Applied and Environental Microbiology,2011,77:2527~2530.
[6]Exall K,Balakrishnan V K,Toito J,et al.Impact of selected wastewater constituents on the removal of sulfonamide antibiotics via ultrafiltration and micellar enhanced ultrafiltration[J].Science of the Total Environment,2013,461~462:371~376.
[7]Warman P R,Thomas R L.Chlortetracycline in soil amended with poultry manure[J].Canadian Journal of Soil Science,1981,61:161~163.
[8]Cengiz M,Balcioglu I,Oruc H H.Detection of Oxytetracycline and Chlortetracycline Residues in Agricultural Fields in Turkey[J].Journal of Biological&Environmental Sciences,2010,4:23~27.
[9]Hamscher G,Sczesny S,H9per H,et al.Determination of persistent tetracycline residues in soil fertilized with liquid manure by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry[J].Analytical Chemistry,2002,74:1509~1518.
[10]Xu W H,Zhang G,Zou S C,et al.A preliminary investigation on the occurrence and distribution of antibiotics in the Yellow River and its tributaries,China[J].Water Environment Research,2009,81:248~254.
[11]Huerta B,Marti E,Gros M,et al.Exploring the links between antibiotic occurrence,antibiotic resistance,and bacterial communities in water supply reservoirs[J].Science of the Total Environment,456~457:161~170.
[12]叶赛,张奎文,姚子伟,等.环渤海水域磺胺类药物的含量特征[J].大连海事大学学报,2007,33(2):71~74.
[13]徐维海,张干,邹世春,等.典型抗生素类药物在城市污水处理厂中的含量水平及其行为特征[J].环境科学,2007,28(8):1779~1783.
[14]Gulkowska A,Sander M,Hollender J,et al.Covalent Binding of Sulfamethazine to Natural and Synthetic Humic Acids:Assessing Laccase Catalysis and Covalent Bond Stability[J].Environmental Science&Technology,2013,47:6916~6924.
[15]Kopmann C,Jechalke S,Rosendahl I,et al.Abundance and transferability of antibiotic resistance as related to the fate of sulfadiazine in maize rhizosphere and bulk soil[J].FEMS Microbiology Ecology,2013,83:125~134.
[16]Pawelzick H T,H9per H,Nau H,et al.A survey of the occurrence of various tetracyclines and sulfamethazine in sandy soils in northwestern Germany fertilized with liquid manure[A].SETAC Euro 14th Annual meeting[C].Prague:Czech Republic,2004:18~22.
[17]ChatterjeeT,Chatterjee B K,Majumdar D,et al.Antibacterial effect of silver nanoparticles and the modeling of bacterial growth kinetics using a modified Gompertz model[J].Biochimica et Biophysica Acta(BBA)-General Subjects,2015,1850:299~306.