碳源补充方式对潮汐流人工湿地生物蓄磷/磷移除的影响
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摘要
采用生物蓄磷/磷移除型潮汐流人工湿地(TFCW)试验装置,探究了周期性磷移除操作过程中3种不同的有机碳源补充方式对系统运行性能的影响.结果表明:碳源补充方式可显著影响TFCW的生物蓄磷/磷移除性能.碳源补充方式的优化有利于系统中聚磷菌群(PAOs)丰度及其活性的维持,可最大限度地提高PAOs在磷移除操作过程中的磷素释放量、胞内聚β-羟基丁酸(PHB)合成量及其对碳源的利用率,并可保障系统在生物蓄磷阶段高效且稳定的除磷效果.当生物蓄磷/磷移除(PB-PH)周期为30 d,且采用连续循环投加方式补充碳源时,TFCW在试验阶段的磷素截留量与磷素移除率分别为26994.88 mg和70.8%.典型PB-PH周期内系统在生物蓄磷过程中的磷素去除率为(91.4±2.1)%,系统中的PAOs在磷移除过程中的磷素释放量、胞内PHB合成量及其对碳源的利用率分别为(1563.72±127.84)mg、(4.52±0.39)mmol C·g-1VSS和(97.3±1.6)%.
We explored the effects of carbon supplementary modes on operational performance in a tidal flow constructed wetland( TFCW) utilizing phosphorus bioaccumulation/phosphorus harvesting( PB-PH) process. Three different carbon supplementary modes were adopted during the periodical phosphorus harvesting process. The results showed that the carbon supplementary mode significantly affected the performances of phosphorus bioaccumulation and phosphorus harvesting throughout the experiment. The optimization of carbon supplementary mode would facilitate the maintenance of abundance and activities of polyphosphate-accumulating organisms( PAOs). Subsequently,the amounts of phosphorus release and PHB synthesis in PAOs,as well as the utilization rate of supplementary carbon,could be effectively enhanced during the phosphorus harvesting process. The efficient and stable phosphorus removal effects of the TFCW could be ensured during the phosphorus bioaccumulation process. When the PB-PH cycle length was 30 d and using the continuous circulation carbon supplementary mode,the amount of phosphorus retained in the bed and the mean phosphorus harvesting efficiency of the TFCW were 26994.88 mg and 70.8%,respectively. Regarding a typical PB-PH cycle,the mean phosphorus removal efficiency of the TFCW could achieve( 91.4 ±2.1) % during the phosphorus bioaccumulation process. The amounts of phosphorus release and PHB synthesis in PAOs,and the utilization rate of supplementary carbon in the system could reach up to( 1563.72±127.84) mg,( 4.52±0.39) mmol C·g-1 VSS,and( 97.3±1.6) %,respectively.
We explored the effects of carbon supplementary modes on operational performance in a tidal flow constructed wetland( TFCW) utilizing phosphorus bioaccumulation/phosphorus harvesting( PB-PH) process. Three different carbon supplementary modes were adopted during the periodical phosphorus harvesting process. The results showed that the carbon supplementary mode significantly affected the performances of phosphorus bioaccumulation and phosphorus harvesting throughout the experiment. The optimization of carbon supplementary mode would facilitate the maintenance of abundance and activities of polyphosphate-accumulating organisms( PAOs). Subsequently,the amounts of phosphorus release and PHB synthesis in PAOs,as well as the utilization rate of supplementary carbon,could be effectively enhanced during the phosphorus harvesting process. The efficient and stable phosphorus removal effects of the TFCW could be ensured during the phosphorus bioaccumulation process. When the PB-PH cycle length was 30 d and using the continuous circulation carbon supplementary mode,the amount of phosphorus retained in the bed and the mean phosphorus harvesting efficiency of the TFCW were 26994.88 mg and 70.8%,respectively. Regarding a typical PB-PH cycle,the mean phosphorus removal efficiency of the TFCW could achieve( 91.4 ±2.1) % during the phosphorus bioaccumulation process. The amounts of phosphorus release and PHB synthesis in PAOs,and the utilization rate of supplementary carbon in the system could reach up to( 1563.72±127.84) mg,( 4.52±0.39) mmol C·g-1 VSS,and( 97.3±1.6) %,respectively.
引文
[1]Melia PM,Cundy AB,Sohi SP,et al.Trends in the recovery of phosphorus in bioavailable forms from wastewater.Chemosphere,2017,186:381-395
[2]Tian Q,Zhuang L,Ong SK,et al.Phosphorus(P)recovery coupled with increasing influent ammonium facilitated intracellular carbon source storage and simultaneous aerobic phosphorus&nitrogen removal.Water Research,2017,119:267-275
[3]Johansson S,Ruscalleda M,Colprim J.Phosphorus recovery through biologically induced precipitation by partial nitritation-anammox granular biomass.Chemical Engineering Journal,2017,327:881-888
[4]Zou H,Wang Y.Phosphorus removal and recovery from domestic wastewater in a novel process of enhanced biological phosphorus removal coupled with crystallization.Bioresource Technology,2016,211:87-92
[5]Zhang Z-J(张志剑),Zhou L-Q(周林强),Li H(李慧),et al.Effectivenesses and mechanism of enhanced biological phosphorus removal(EBPR)of municipal wastewater treatment plants.China Environmental Science(中国环境科学),2010,30(12):1614-1621(in Chinese)
[6]Kodera H,Hatamoto M,Abe K,et al.Phosphate recovery as concentrated solution from treated wastewater by a PAO-enriched biofilm reactor.Water Research,2013,47:2025-2032
[7]Zhou L(周律),Li G(李哿),SHINHang-sik,et al.Characteristics of nitrogen and phosphorus removal and mocrobial community structure in hybrid biofilm system.Acta Scientiae Circumstantiae(环境科学学报),2012,32(6):1312-1318(in Chinese)
[8]Wang F-R(王凤蕊),Zhang S(张顺),Tian Q(田晴),et al.Biofilm characteristics of an anaerobic/aerobic biofilter varied with carbon source regulation.Microbiology China(微生物学通报),2013,40(12):2227-2236(in Chinese)
[9]Wang Z(王振),Qi R(齐冉),Li Y-Y(李莹莹),et al.Improvement of phosphorus bioaccumulation in a tidal flow constructed wetland and its stability.China Environmental Science(中国环境科学),2017,37(2):534-542(in Chinese)
[10]Zhang C(张成),Qin H-X(秦华星),Wang K-W(王康伟),et al.Effects of carbon source supplement patterns on phosphorus bio-accumulation and harvesting from an alternating anaerobic/aerobic biofilter.Chinese Journal of Environmental Engineering(环境工程学报),2015,9(8):3602-3608(in Chinese)
[11]Ma F(马飞),Jiang L(蒋莉),Xiong J-Y(熊洁羽),et al.Changes of hydraulics of constructed wetlands after backwashing.Environmental Science&Technology(环境科学与技术),2011,34(7):46-49(in Chinese)
[12]Guo M-K(郭明昆),Wu C-Y(吴昌永),Zhou Y-X(周岳溪),et al.Optimization of backwashing conditions and sludge characteristics in enhanced phosphorus removal biological aerated filter.Research of Environmental Sciences(环境科学研究),2016,29(3):404-410(in Chinese)
[13]General Administration of Environmental Protection of the People’s Republic of China(国家环境保护总局).Standard Methods for the Examination of Water and Wastewater.Beijing:China Environmental Science Press,2002(in Chinese)
[14]Fu W(傅威),Ye J-F(叶建锋),Gu H(顾虹),et al.Analysis on accumulation and transformation of organic matter in vertical flow constructed wetlands.Environmental Pollution&Control(环境污染与防治),2010,32(3):55-59(in Chinese)
[15]Oehmen A,Keller-Lehmann B,Zeng RJ,et al.Optimisation of poly-β-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems.Journal of Chromatography A,2005,1070:131-136
[16]Crocetti GR,Hugenholtz P,Bond PL,et al.Identification of polyphosphate-accumulating organisms and design of 16S rRNA directed probes for their detection and quantitation.Applied and Environmental Microbiology,2000,66:1175-1182
[17]Kong Y,Xia Y,Nielsen JL,et al.Structure and function of the microbial community in a full-scale enhanced biological phosphorus removal plant.Microbiology,2007,153:4061-4073
[18]Wang Z(王振),Liu C-X(刘超翔),Li P-Y(李鹏宇),et al.Study on phosphorus removal capability of constructed wetlands filled with broken bricks.Environmental Science(环境科学),2012,33(12):4373-4379(in Chinese)
[19]General Administration of Environmental Protection of the People’s Republic of China(国家环境保护总局).Soil Agro-chemistrical Analysis.Beijing:China Environmental Science Press,1986(in Chinese)
[20]Wu S,Kuschk P,Brix H,et al.Development of constructed wetlands in performance intensifications for wastewater treatment:A nitrogen and organic matter targeted review.Water Research,2014,57:40-55
[21]Li L,He C,Ji G,et al.Nitrogen removal pathways in a tidal flow constructed wetland under flooded time constraints.Ecological Engineering,2015,81:266-271
[22]Wu S,Zhang D,Austin D,et al.Evaluation of a labscale tidal flow constructed wetland performance:Oxygen transfer capacity,organic matter and ammonium removal.Ecological Engineering,2011,37:1789-1795
[23]Liu M-H(柳明慧),Wu S-B(吴树彪),Ju X-X(鞠鑫鑫),et al.Progress and prospects of polluted water treatment of tidal flow constructed wetland.Technology of Water Treatment(水处理技术),2014,40(5):10-15(in Chinese)
[24]Fang Z-D(方振东),Xian G(仙光),Long X-Y(龙向宇),et al.Effect of phosphate species in extracellular polymer substances on biological phosphorus removal process.Acta Scientiae Circumstantiae(环境科学学报),2014,34(9):2205-2212(in Chinese)
[25]Roy ED.Phosphorus recovery and recycling with ecological engineering:A review.Ecological Engineering,2017,98:213-227
[26]Zheng X,Sun P,Han J,et al.Inhibitory factors affecting the process of enhanced biological phosphorus removal(EBPR):A mini-review.Process Biochemistry,2014,49:2207-2213
[27]Tang X-G(唐旭光),Wang S-Y(王淑莹),Peng Y-Z(彭永臻),et al.Effect of different modes of influent to anaerobic phosphorus release in EPBR.Environmental Engineering(环境工程),2008,26(Suppl.1):36-40(in Chinese)
[28]Peng Y-Z(彭永臻),Lyu D-M(吕冬梅),Wang S-Y(王淑莹).Effects of feed water COD and different adding ways of COD on denitrifying phosphorus removal of A2O-BAF process.Journal of Beijing University of Technology(北京工业大学学报),2016,42(9):1406-1413(in Chinese)
[29]Chen H-B(陈洪斌),Tang X-C(唐贤春),He Q-B(何群彪),et al.Phosphorus uptaking behavior of phosphorus accumulation organisms in reversed AAO Process.China Environmental Science(中国环境科学),2007,27(1):49-53(in Chinese)
[30]Sheng X-Y(盛欣英),Xiong H-L(熊惠磊),Sun R(孙润),et al.Polyhydroxyalkanoates production by Ralstonia eutropha H16 fed by waste sludge anaerobic fermentative liquid.China Environmental Science(中国环境科学),2012,32(11):2047-2052(in Chinese)
[31]Zhang S(张顺),Tian Q(田晴),Tang M-L(汤曼琳),et al.Effect of phosphorus recovery on phosphorous bioaccumulation/harvesting in an alternating anaerobic/aerobic biofilter system.Environmental Science(环境科学),2014,35(3):979-986(in Chinese)
[32]Feng C-J(冯翠杰),Wang S-M(王淑梅),Chen S-H(陈少华).Research progress of competition and control between PAOs and GAOs for different carbon sources.Chemical Industry and Engineering Progress(化工进展),2011,30(1):196-202(in Chinese)
[33]Liao X-D(廖新俤),Luo S-M(骆世明),Wu Y-B(吴银宝),et al.Comparison of nutrient removal ability between Cyperus alternifolius in constructed wetlands.Chinese Journal of Applied Ecology(应用生态学报),2005,16(1):156-160(in Chinese)
[34]Wang Q-H(王庆海),Duan L-S(段留生),Wu J-Y(武菊英),et al.Growth vitality and pollutants-removal ability of plants in constructed wetland in Beijing region.Chinese Journal of Applied Ecology(应用生态学报),2008,19(5):1131-1137(in Chinese)
[35]Saeed T,Sun G.A review on nitrogen and organics removal mechanisms in subsurface flow constructed wetlands:Dependency on environmental parameters,operating conditions and supporting media.Journal of Environmental Management,2012,112:429-448
[2]Tian Q,Zhuang L,Ong SK,et al.Phosphorus(P)recovery coupled with increasing influent ammonium facilitated intracellular carbon source storage and simultaneous aerobic phosphorus&nitrogen removal.Water Research,2017,119:267-275
[3]Johansson S,Ruscalleda M,Colprim J.Phosphorus recovery through biologically induced precipitation by partial nitritation-anammox granular biomass.Chemical Engineering Journal,2017,327:881-888
[4]Zou H,Wang Y.Phosphorus removal and recovery from domestic wastewater in a novel process of enhanced biological phosphorus removal coupled with crystallization.Bioresource Technology,2016,211:87-92
[5]Zhang Z-J(张志剑),Zhou L-Q(周林强),Li H(李慧),et al.Effectivenesses and mechanism of enhanced biological phosphorus removal(EBPR)of municipal wastewater treatment plants.China Environmental Science(中国环境科学),2010,30(12):1614-1621(in Chinese)
[6]Kodera H,Hatamoto M,Abe K,et al.Phosphate recovery as concentrated solution from treated wastewater by a PAO-enriched biofilm reactor.Water Research,2013,47:2025-2032
[7]Zhou L(周律),Li G(李哿),SHINHang-sik,et al.Characteristics of nitrogen and phosphorus removal and mocrobial community structure in hybrid biofilm system.Acta Scientiae Circumstantiae(环境科学学报),2012,32(6):1312-1318(in Chinese)
[8]Wang F-R(王凤蕊),Zhang S(张顺),Tian Q(田晴),et al.Biofilm characteristics of an anaerobic/aerobic biofilter varied with carbon source regulation.Microbiology China(微生物学通报),2013,40(12):2227-2236(in Chinese)
[9]Wang Z(王振),Qi R(齐冉),Li Y-Y(李莹莹),et al.Improvement of phosphorus bioaccumulation in a tidal flow constructed wetland and its stability.China Environmental Science(中国环境科学),2017,37(2):534-542(in Chinese)
[10]Zhang C(张成),Qin H-X(秦华星),Wang K-W(王康伟),et al.Effects of carbon source supplement patterns on phosphorus bio-accumulation and harvesting from an alternating anaerobic/aerobic biofilter.Chinese Journal of Environmental Engineering(环境工程学报),2015,9(8):3602-3608(in Chinese)
[11]Ma F(马飞),Jiang L(蒋莉),Xiong J-Y(熊洁羽),et al.Changes of hydraulics of constructed wetlands after backwashing.Environmental Science&Technology(环境科学与技术),2011,34(7):46-49(in Chinese)
[12]Guo M-K(郭明昆),Wu C-Y(吴昌永),Zhou Y-X(周岳溪),et al.Optimization of backwashing conditions and sludge characteristics in enhanced phosphorus removal biological aerated filter.Research of Environmental Sciences(环境科学研究),2016,29(3):404-410(in Chinese)
[13]General Administration of Environmental Protection of the People’s Republic of China(国家环境保护总局).Standard Methods for the Examination of Water and Wastewater.Beijing:China Environmental Science Press,2002(in Chinese)
[14]Fu W(傅威),Ye J-F(叶建锋),Gu H(顾虹),et al.Analysis on accumulation and transformation of organic matter in vertical flow constructed wetlands.Environmental Pollution&Control(环境污染与防治),2010,32(3):55-59(in Chinese)
[15]Oehmen A,Keller-Lehmann B,Zeng RJ,et al.Optimisation of poly-β-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems.Journal of Chromatography A,2005,1070:131-136
[16]Crocetti GR,Hugenholtz P,Bond PL,et al.Identification of polyphosphate-accumulating organisms and design of 16S rRNA directed probes for their detection and quantitation.Applied and Environmental Microbiology,2000,66:1175-1182
[17]Kong Y,Xia Y,Nielsen JL,et al.Structure and function of the microbial community in a full-scale enhanced biological phosphorus removal plant.Microbiology,2007,153:4061-4073
[18]Wang Z(王振),Liu C-X(刘超翔),Li P-Y(李鹏宇),et al.Study on phosphorus removal capability of constructed wetlands filled with broken bricks.Environmental Science(环境科学),2012,33(12):4373-4379(in Chinese)
[19]General Administration of Environmental Protection of the People’s Republic of China(国家环境保护总局).Soil Agro-chemistrical Analysis.Beijing:China Environmental Science Press,1986(in Chinese)
[20]Wu S,Kuschk P,Brix H,et al.Development of constructed wetlands in performance intensifications for wastewater treatment:A nitrogen and organic matter targeted review.Water Research,2014,57:40-55
[21]Li L,He C,Ji G,et al.Nitrogen removal pathways in a tidal flow constructed wetland under flooded time constraints.Ecological Engineering,2015,81:266-271
[22]Wu S,Zhang D,Austin D,et al.Evaluation of a labscale tidal flow constructed wetland performance:Oxygen transfer capacity,organic matter and ammonium removal.Ecological Engineering,2011,37:1789-1795
[23]Liu M-H(柳明慧),Wu S-B(吴树彪),Ju X-X(鞠鑫鑫),et al.Progress and prospects of polluted water treatment of tidal flow constructed wetland.Technology of Water Treatment(水处理技术),2014,40(5):10-15(in Chinese)
[24]Fang Z-D(方振东),Xian G(仙光),Long X-Y(龙向宇),et al.Effect of phosphate species in extracellular polymer substances on biological phosphorus removal process.Acta Scientiae Circumstantiae(环境科学学报),2014,34(9):2205-2212(in Chinese)
[25]Roy ED.Phosphorus recovery and recycling with ecological engineering:A review.Ecological Engineering,2017,98:213-227
[26]Zheng X,Sun P,Han J,et al.Inhibitory factors affecting the process of enhanced biological phosphorus removal(EBPR):A mini-review.Process Biochemistry,2014,49:2207-2213
[27]Tang X-G(唐旭光),Wang S-Y(王淑莹),Peng Y-Z(彭永臻),et al.Effect of different modes of influent to anaerobic phosphorus release in EPBR.Environmental Engineering(环境工程),2008,26(Suppl.1):36-40(in Chinese)
[28]Peng Y-Z(彭永臻),Lyu D-M(吕冬梅),Wang S-Y(王淑莹).Effects of feed water COD and different adding ways of COD on denitrifying phosphorus removal of A2O-BAF process.Journal of Beijing University of Technology(北京工业大学学报),2016,42(9):1406-1413(in Chinese)
[29]Chen H-B(陈洪斌),Tang X-C(唐贤春),He Q-B(何群彪),et al.Phosphorus uptaking behavior of phosphorus accumulation organisms in reversed AAO Process.China Environmental Science(中国环境科学),2007,27(1):49-53(in Chinese)
[30]Sheng X-Y(盛欣英),Xiong H-L(熊惠磊),Sun R(孙润),et al.Polyhydroxyalkanoates production by Ralstonia eutropha H16 fed by waste sludge anaerobic fermentative liquid.China Environmental Science(中国环境科学),2012,32(11):2047-2052(in Chinese)
[31]Zhang S(张顺),Tian Q(田晴),Tang M-L(汤曼琳),et al.Effect of phosphorus recovery on phosphorous bioaccumulation/harvesting in an alternating anaerobic/aerobic biofilter system.Environmental Science(环境科学),2014,35(3):979-986(in Chinese)
[32]Feng C-J(冯翠杰),Wang S-M(王淑梅),Chen S-H(陈少华).Research progress of competition and control between PAOs and GAOs for different carbon sources.Chemical Industry and Engineering Progress(化工进展),2011,30(1):196-202(in Chinese)
[33]Liao X-D(廖新俤),Luo S-M(骆世明),Wu Y-B(吴银宝),et al.Comparison of nutrient removal ability between Cyperus alternifolius in constructed wetlands.Chinese Journal of Applied Ecology(应用生态学报),2005,16(1):156-160(in Chinese)
[34]Wang Q-H(王庆海),Duan L-S(段留生),Wu J-Y(武菊英),et al.Growth vitality and pollutants-removal ability of plants in constructed wetland in Beijing region.Chinese Journal of Applied Ecology(应用生态学报),2008,19(5):1131-1137(in Chinese)
[35]Saeed T,Sun G.A review on nitrogen and organics removal mechanisms in subsurface flow constructed wetlands:Dependency on environmental parameters,operating conditions and supporting media.Journal of Environmental Management,2012,112:429-448