EGTA与有机酸联合施用对黄麻修复Cd污染土壤的影响
|
摘要
采用盆栽试验研究了柠檬酸(CA)和酒石酸(TA)分别与螯合剂乙二醇双(2-氨基乙基醚)四乙酸(EGTA)的配施对改进黄麻部分生理特性和Cd提取效率的影响。结果表明,EGTA与有机酸配施能促进黄麻光合色素的合成,增加抗氧化酶活性。各处理均提高了黄麻的株高、根长和各器官的干质量,T2E2处理的总干质量最大,为对照的2.69倍。EGTA与CA和TA的配施不同程度提高了黄麻各器官中的Cd含量和积累量,在E1C1处理中,黄麻具有最大的地上部Cd积累量643.02μg·plant-1。各处理地上部和地下部富集系数均有所提高,地上部增幅达1.30～2.14倍,地下部增幅达0.11～0.80倍。各处理的迁移系数分别较CK增加了0.64～1.66倍,其中,T1E2处理的迁移系数最高,达3.15。土壤净化率明显提高,在T2E2和C1E1处理中达最大值0.346%。综上所述,EGTA与TA或CA配施能显著改善黄麻对Cd的提取效率。
In this study, we focused on the effects of combined application of chelating agents and organic acid on Cd absorption and accumulation by Corchorus capsularis L. Pot experiments were conducted to investigate the effects of citric acid(CA)and tartaric acid(TA)combined with EGTA on physiological characteristics and Cd accumulation in C. capsularis. The results showed that the application of EG?TA and organic acids promoted the synthesis of photosynthetic pigments and increased the activity of antioxidant enzymes in C. capsularis.All treatments increased plant height, root length and biomass of C. capsularis, and the maximum biomass of plant when the EGTA and TAwere both at 2 mol·L-1 T2 E2 treatment(EGTA and TA were both at 2 mol·L-1)was 2.69 higher than that of the control. The combination ofEGTA with CA and TA increased the concentration and accumulation of Cd in different organs of C. capsularis, and the highest shoot Cd ac?cumulation reached 643.02 μg·plant-1 with the C1 E1 treatment(EGTA and CA were both at 1 mol·L-1). The bioconcentration factor of theroot and shoot improved after the treatments, with an increase of 1.30～2.14 times and 0.11～0.80 times, respectively. With the application ofEGTA and organic acid, the transportation factor increased by 0.64～1.66 times, and reached a maximum of 3.15. The Cd purification ratewas significantly increased, reaching a maximum of 0.346% in the T2 E2 and C1 E1 treatments, which 7.69 times higher than that of the control. The overall results demonstrate that the application of EGTA and organic acids together improve the phytoextraction in C. capsularis.
In this study, we focused on the effects of combined application of chelating agents and organic acid on Cd absorption and accumulation by Corchorus capsularis L. Pot experiments were conducted to investigate the effects of citric acid(CA)and tartaric acid(TA)combined with EGTA on physiological characteristics and Cd accumulation in C. capsularis. The results showed that the application of EG?TA and organic acids promoted the synthesis of photosynthetic pigments and increased the activity of antioxidant enzymes in C. capsularis.All treatments increased plant height, root length and biomass of C. capsularis, and the maximum biomass of plant when the EGTA and TAwere both at 2 mol·L-1 T2 E2 treatment(EGTA and TA were both at 2 mol·L-1)was 2.69 higher than that of the control. The combination ofEGTA with CA and TA increased the concentration and accumulation of Cd in different organs of C. capsularis, and the highest shoot Cd ac?cumulation reached 643.02 μg·plant-1 with the C1 E1 treatment(EGTA and CA were both at 1 mol·L-1). The bioconcentration factor of theroot and shoot improved after the treatments, with an increase of 1.30～2.14 times and 0.11～0.80 times, respectively. With the application ofEGTA and organic acid, the transportation factor increased by 0.64～1.66 times, and reached a maximum of 3.15. The Cd purification ratewas significantly increased, reaching a maximum of 0.346% in the T2 E2 and C1 E1 treatments, which 7.69 times higher than that of the control. The overall results demonstrate that the application of EGTA and organic acids together improve the phytoextraction in C. capsularis.
引文
[1]Chen H,Teng Y,Lu S,et al.Contamination features and health risk of soil heavy metals in China[J].Science of the Total Environment,2015,512-513:143-153.
[2]Ran X,Shuang W,Li R,et al.Soil heavy metal contamination and health risks associated with artisanal gold mining in Tongguan,Shaanxi,China[J].Ecotoxicology&Environmental Safety,2017,141:17-24.
[3]Jaishankar M,Tseten T,Anbalagan N,et al.Toxicity mechanism and health effects of some heavy metals[J].Interdisciplinary Toxicology,2014,7(2):60-72.
[4]孙鑫,娄燕宏,王会,等.重金属污染土壤的植物强化修复研究进展[J].土壤通报,2017,48(4):1008-1013.SUN Xin,LOU Yan-hong,WANG Hui,et al.Review on enhancing phytoremediation of soil contamination by heavy metals[J].Chinese Journal of Soil Science,2017,48(4):1008-1013.
[5]Sarwar N,Imran M,Shaheen M R,et al.Phytoremediation strategies for soils contaminated with heavy metals:Modifications and future perspectives[J].Chemosphere,2017,171:710-721.
[6]Cristaldi A,Conti G O,Jho E H,et al.Phytoremediation of contaminated soils by heavy metals and PAHs:A brief review[J].Environmental Technology&Innovation,2017,8:309-326.
[7]陈良华,徐睿,张健,等.螯合剂对香樟生理特征和Cd积累效率的影响[J].云南大学学报(自然科学版),2016,38(1):150-161.CHEN Liang-hua,XU Rui,ZHANG Jian,et al.The effects of chelator on physiological traits and Cd accumulation efficiency of Cinnamomum camphora[J].Journal of Yunnan University(Natural Science),2016,38(1):150-161.
[8]魏树和,周启星.重金属污染土壤植物修复基本原理及强化措施探讨[J].生态学杂志,2004,23(1):65-72.WEI Shu-he,ZHOU Qi-xing.Discussion on basic principles and strengthening measures for phytoremediation of soils contaminated by heavy metals[J].Chinese Journal of Ecology,2004,23(1):65-72.
[9]陈亚慧,李君,王明新,等.EGTA和酒石酸对蓖麻Cd胁迫与积累的调控作用[J].西北植物学报,2014,35(9):1025-1031.CHEN Ya-hui,LI Jun,WANG Ming-xin,et al.Regulation of EGTAand tartaric acid on Cd stress and accumunition in Ricinus communis L.[J].Acta Botanica Boreali-Occidentalia Sinica,2014,35(9):1025-1031.
[10]胡亚虎,魏树和,周启星,等.螯合剂在重金属污染土壤植物修复中的应用研究进展[J].农业环境科学学报,2010,29(11):2055-2063.HU Ya-hu,WEI Shu-he,ZHOU Qi-xing,et al.Application of chelator in phytoremediation of heavy metals contaminated soils:A review[J].Journal of Agro-Environment Science,2010,29(11):2055-2063.
[11]李君,葛跃,王明新,等.EGTA对Cd胁迫下蓖麻Cd积累和营养元素吸收的影响[J].西北植物学报,2015,35(9):1855-1860.LI Jun,GE Yue,WANG Ming-xin,et al.Regulation of EGTA on Cd and nutrient elements accumulation in Ricinus Communis under Cd stress[J].Acta Botanica Boreali-Occidentalia Sinica.2015,35(9):1855-1860.
[12]Hu Y H,Nan Z R,Su J Q,et al.Chelant-assisted uptake and accumulation of cd by poplar from calcareous arable soils around Baiyin nonferrous metal smelters,Northern China[J].Arid Soil Research&Rehabilitation,2014,28(3):340-354.
[13]Wang S,Liu J.The effectiveness and risk comparison of EDTA with EGTA in enhancing Cd phytoextraction by Mirabilis jalapa L.[J].Environmental Monitoring and Assessment,2014,186(2):751-759.
[14]Parmar P,Kumari N,Sharma V.Structural and functional alterations in photosynthetic apparatus of plants under cadmium stress[J].Botanical Studies,2013,54(1):45.
[15]Afshan S,Ali S,Bharwana S A,et al.Citric acid enhances the phytoextraction of chromium,plant growth,and photosynthesis by alleviating the oxidative damages in Brassica napus L.[J].Environmental Science and Pollution Research,2015,22(15):11679-11689.
[16]杨艳,汪敏,刘雪云,等.三种有机酸对Cd胁迫下油菜生理特性的影响[J].安徽师范大学学报(自然科学版),2007(2):158-162.YANG Yan,WANG Min,LIU Xue-yun,et al.Effects of organic acids on the growth of rape under Cd stress[J].Journal of Anhui Normal University(Natural Science),2007(2):158-162.
[17]董袁媛,孙竹,杨洋,等.镉胁迫对黄麻光合作用及镉积累的影响[J].核农学报,2017,31(8):1640-1646.DONG Yuan-yuan,SUN Zhu,YANG Yang,et al.Effects of cadmium of photosynthesis and Cd accumulation of Corchorus capsularis L.[J].Journal of Nuclear Agricultural Sciences,2017,31(8):1640-1646.
[18]王玉富.黄麻、红麻在重金属污染耕地修复中的应用研究进展[J].湖南农业科学,2015(8):49-52.WANG Yu-fu.Review of jute and kenaf application in remediation of arable lands contaminated by heavy metals[J].Hunan Agricultural Sciences,2015(8):49-52.
[19]熊庆娥.植物生理学实验教程[M].成都:四川科学技术出版社,2003:52-124.XIONG Qing-e.Plant physiology[M].Chengdu:Sichuan Science and Technology Press,2003:52-124.
[20]刘凤枝,李玉浸.土壤监测分析技术[M].北京:化学工业出版社,2015:198-341.LIU Feng-zhi,LI Yu-jin.Soil monitoring and analysis technology[M].Beijing:Chemical Industry Press,2015:198-341.
[21]张朝阳,彭平安,宋建中,等.改进BCR法分析国家土壤标准物质中重金属化学形态[J].生态环境学报,2012,21(11):1881-1884.ZHANG Chao-yang,PENG Ping-an,SONG Jian-zhong,et al.Utilization of modified BCR procedure for the chemical speciation of heavy metals in Chinese soil reference material[J].Ecology&Environmental Sciences,2012,21(11):1881-1884.
[22]刘铭,刘凤枝,刘保峰.土壤中有效态铅和镉的测定[J].农业环境科学学报,2007,26(S1):300-302.LIU Ming,LIU Feng-zhi,LIU Bao-feng.Determination of available lead and cadmium in soil[J].Journal of Agro-Environment Science,2007,26(S1):300-302.
[23]徐小逊,董袁媛,邓玉兰,等.镉胁迫对豨莶生长及光合作用相关参数的影响[J].农业环境科学学报,2016,35(9):1672-1679.XU Xiao-xun,DONG Yuan-yuan,DENG Yu-lan,et al.Effects of cadmium stress on growth and photosynthetic parameters of Sigesbeckia orientalis L.[J].Journal of Agro-Environment Science,2016,35(9):1672-1679.
[24]Cay S,Uyanik A,Engin M S.EDTA supported phytoextraction of Cd from contaminated soil by four different ornamental plant species[J].Journal of Soil Contamination,2016,25(3):346-355.
[25]张磊.螯合剂强化棉花对镉污染土壤修复的初步研究[J].水土保持学报,2015,29(4):321-326.ZHANG Lei.Preliminary study on chelate-enhanced phytoremediation of cadmium-contami nated soil by cotton plants[J].Journal of Soil and Water Conservation,2015,29(4):321-326.
[26]陈英旭,林琦,陆芳,等.有机酸对铅、镉植株危害的解毒作用研究[J].环境科学学报,2000,20(4):467-472.CHEN Ying-xu,LIN Qi,LU Fang,et al.Study on detoxication of organic acid to raddish under the stress of Pb and Cd[J].Acta Scientiae Circumstantiae,2000,20(4):467-472.
[27]夏小燕,杨丽琴,翟福勤,等.有机酸对小麦幼苗镉毒的缓解作用[J].农业环境科学学报,2007,26(3):990-995.XIA Xiao-yan,YANG Li-qin,ZHAI Fu-qin,et al.Organic acids alleviating the toxicity of Cd to young wheat plants[J].Journal of AgroEnvironment Science,2007,26(3):990-995.
[28]Zaheer I E,Ali S,Rizwan M,et al.Citric acid assisted phytoremediation of copper by Brassica napus L.[J].Ecotoxicology and Environmental Safety,2015,120:310-317.
[29]张玉芬,刘景辉,杨彦明,等.柠檬酸和EDTA对蓖麻生理特性和镉累积的影响[J].生态与农村环境学报,2015,31(5):760-766.ZHANG Yu-fen,LIU Jing-hui,YANG Yan-ming,et al.Effects of CA and EDTA on physiological characteristics and Cd accumulation of Ricinus communis[J].Journal of Ecology and Rural Environment,2015,31(5):760-766.
[30]胡国涛,杨兴,陈小米,等.速生树种竹柳对重金属胁迫的生理响应[J].环境科学学报,2016,36(10):3870-3875.HU Guo-tao,YANG Xing,CHEN Xiao-mi,et al.Physiological responses of bamboo-willow plants to heavy metal stress[J].Acta Scientiae Circumstantiae,2016,36(10):3870-3875.
[31]王思予,多立安,赵树兰.可降解螯合剂对草坪植物高羊茅发育及生理的影响[J].园艺学报,2017,44(11):2186-2194.WANG Si-yu,DUO Li-an,ZHAO Shu-lan.Effects of biodegradable chelator on growth and physiology of Festuca arundinacea seedlings[J].Acta Horticulturae Sinica,2017,44(11):2186-2194.
[32]Huang C,Lai C,Xu P,et al.Lead-induced oxidative stress and antioxidant response provide insight into the tolerance of Phanerochaete chrysosporium to lead exposure[J].Chemosphere,2017,187:70-77.
[33]Zhao Y,Mao G,Hu H,et al.Effects of EDTA and EDDS on heavy metal activation and accumulation of metals by soybean in alkaline soil[J].Journal of Soil Contamination,2015,24(4):353-367.
[34]Gao Y,Miao C,Xia J,et al.Effect of citric acid on phytoextraction and antioxidative defense in Solanum nigrum L.as a hyperaccumulator under Cd and Pb combined pollution[J].Environmental Earth Sciences,2012,65(7):1923-1932.
[35]Ehsan S,Ali S,Noureen S,et al.Citric acid assisted phytoremediation of cadmium by Brassica napus L.[J].Ecotoxicology&Environmental Safety,2014,106:164-172.
[36]熊国焕,潘义宏,何艳明,等.螯合剂对大叶井口边草Pb、Cd、As吸收性影响研究[J].土壤,2012,44(2):282-289.XIONG Guo-huan,PAN Yi-hong,HE Yan-ming,et al.Chelate assisted uptake of heavy metal of lead,cadmium and arsenic from soil with Pteris cretica var.nervosa[J].Soils,2012,44(2):282-289.
[37]张譞,李晔,胡进,等.三种螯合剂对土壤重金属Cd和Zn形态变化的研究[J].科学技术与工程,2013,13(21):6184-6188.ZHANG Xuan,LI Ye,HU Jin,et al.Effects of three chelate on the form distribution of Cd and Zn in soil[J].Science Technology and Engineering,2013,13(21):6184-6188.
[38]尹炳奎,黄满红,张大磊,等.菜籽饼施加对镉-铜污染土壤中重金属形态转化及其植物有效性的影响[J].环境工程学报,2017,11(6):3879-3883.YIN Bing-kui,HUANG Man-hong,ZHANG Da-lei,et al.Effects of rapeseed cake on cadmium and copper forms and its phytoavailability in heavy metals contaminated paddy soil[J].Chinese Journal of Environmental Engineering,2017,11(6):3879-3883.
[39]郑明霞,冯流,刘洁,等.螯合剂对土壤中镉赋存形态及其生物有效性的影响[J].环境化学,2007,26(5):606-609.ZHENG Ming-xia,FENG Liu,LIU Jie,et al.Effects of chelators on species and bioavailability of cadmium in soil[J].Environmental Chemistry,2007,26(5):606-609.
[40]李永涛,刘科学,张池,等.广东大宝山地区重金属污染水田土壤的Cu Pb Zn Cd全量与DTPA浸提态含量的相互关系研究[J].农业环境科学学报,2004,23(6):1110-1114.LI Yong-tao,LIU Ke-xue,ZHANG Chi,et al.Relationships between total and DTPA extractable contents of Cu,Pb,Zn,Cd in trace metalcontaminated paddy soils of Dabaoshan,Guangdong[J].Journal of Agro-environmental Science,2004,23(6):1110-1114.
[41]Luo J,Cai L,Qi S,et al.Improvement effects of cytokinin on EDTAassisted phytoremediation and the associated environmental risks[J].Chemosphere,2017,185:386-393.
[2]Ran X,Shuang W,Li R,et al.Soil heavy metal contamination and health risks associated with artisanal gold mining in Tongguan,Shaanxi,China[J].Ecotoxicology&Environmental Safety,2017,141:17-24.
[3]Jaishankar M,Tseten T,Anbalagan N,et al.Toxicity mechanism and health effects of some heavy metals[J].Interdisciplinary Toxicology,2014,7(2):60-72.
[4]孙鑫,娄燕宏,王会,等.重金属污染土壤的植物强化修复研究进展[J].土壤通报,2017,48(4):1008-1013.SUN Xin,LOU Yan-hong,WANG Hui,et al.Review on enhancing phytoremediation of soil contamination by heavy metals[J].Chinese Journal of Soil Science,2017,48(4):1008-1013.
[5]Sarwar N,Imran M,Shaheen M R,et al.Phytoremediation strategies for soils contaminated with heavy metals:Modifications and future perspectives[J].Chemosphere,2017,171:710-721.
[6]Cristaldi A,Conti G O,Jho E H,et al.Phytoremediation of contaminated soils by heavy metals and PAHs:A brief review[J].Environmental Technology&Innovation,2017,8:309-326.
[7]陈良华,徐睿,张健,等.螯合剂对香樟生理特征和Cd积累效率的影响[J].云南大学学报(自然科学版),2016,38(1):150-161.CHEN Liang-hua,XU Rui,ZHANG Jian,et al.The effects of chelator on physiological traits and Cd accumulation efficiency of Cinnamomum camphora[J].Journal of Yunnan University(Natural Science),2016,38(1):150-161.
[8]魏树和,周启星.重金属污染土壤植物修复基本原理及强化措施探讨[J].生态学杂志,2004,23(1):65-72.WEI Shu-he,ZHOU Qi-xing.Discussion on basic principles and strengthening measures for phytoremediation of soils contaminated by heavy metals[J].Chinese Journal of Ecology,2004,23(1):65-72.
[9]陈亚慧,李君,王明新,等.EGTA和酒石酸对蓖麻Cd胁迫与积累的调控作用[J].西北植物学报,2014,35(9):1025-1031.CHEN Ya-hui,LI Jun,WANG Ming-xin,et al.Regulation of EGTAand tartaric acid on Cd stress and accumunition in Ricinus communis L.[J].Acta Botanica Boreali-Occidentalia Sinica,2014,35(9):1025-1031.
[10]胡亚虎,魏树和,周启星,等.螯合剂在重金属污染土壤植物修复中的应用研究进展[J].农业环境科学学报,2010,29(11):2055-2063.HU Ya-hu,WEI Shu-he,ZHOU Qi-xing,et al.Application of chelator in phytoremediation of heavy metals contaminated soils:A review[J].Journal of Agro-Environment Science,2010,29(11):2055-2063.
[11]李君,葛跃,王明新,等.EGTA对Cd胁迫下蓖麻Cd积累和营养元素吸收的影响[J].西北植物学报,2015,35(9):1855-1860.LI Jun,GE Yue,WANG Ming-xin,et al.Regulation of EGTA on Cd and nutrient elements accumulation in Ricinus Communis under Cd stress[J].Acta Botanica Boreali-Occidentalia Sinica.2015,35(9):1855-1860.
[12]Hu Y H,Nan Z R,Su J Q,et al.Chelant-assisted uptake and accumulation of cd by poplar from calcareous arable soils around Baiyin nonferrous metal smelters,Northern China[J].Arid Soil Research&Rehabilitation,2014,28(3):340-354.
[13]Wang S,Liu J.The effectiveness and risk comparison of EDTA with EGTA in enhancing Cd phytoextraction by Mirabilis jalapa L.[J].Environmental Monitoring and Assessment,2014,186(2):751-759.
[14]Parmar P,Kumari N,Sharma V.Structural and functional alterations in photosynthetic apparatus of plants under cadmium stress[J].Botanical Studies,2013,54(1):45.
[15]Afshan S,Ali S,Bharwana S A,et al.Citric acid enhances the phytoextraction of chromium,plant growth,and photosynthesis by alleviating the oxidative damages in Brassica napus L.[J].Environmental Science and Pollution Research,2015,22(15):11679-11689.
[16]杨艳,汪敏,刘雪云,等.三种有机酸对Cd胁迫下油菜生理特性的影响[J].安徽师范大学学报(自然科学版),2007(2):158-162.YANG Yan,WANG Min,LIU Xue-yun,et al.Effects of organic acids on the growth of rape under Cd stress[J].Journal of Anhui Normal University(Natural Science),2007(2):158-162.
[17]董袁媛,孙竹,杨洋,等.镉胁迫对黄麻光合作用及镉积累的影响[J].核农学报,2017,31(8):1640-1646.DONG Yuan-yuan,SUN Zhu,YANG Yang,et al.Effects of cadmium of photosynthesis and Cd accumulation of Corchorus capsularis L.[J].Journal of Nuclear Agricultural Sciences,2017,31(8):1640-1646.
[18]王玉富.黄麻、红麻在重金属污染耕地修复中的应用研究进展[J].湖南农业科学,2015(8):49-52.WANG Yu-fu.Review of jute and kenaf application in remediation of arable lands contaminated by heavy metals[J].Hunan Agricultural Sciences,2015(8):49-52.
[19]熊庆娥.植物生理学实验教程[M].成都:四川科学技术出版社,2003:52-124.XIONG Qing-e.Plant physiology[M].Chengdu:Sichuan Science and Technology Press,2003:52-124.
[20]刘凤枝,李玉浸.土壤监测分析技术[M].北京:化学工业出版社,2015:198-341.LIU Feng-zhi,LI Yu-jin.Soil monitoring and analysis technology[M].Beijing:Chemical Industry Press,2015:198-341.
[21]张朝阳,彭平安,宋建中,等.改进BCR法分析国家土壤标准物质中重金属化学形态[J].生态环境学报,2012,21(11):1881-1884.ZHANG Chao-yang,PENG Ping-an,SONG Jian-zhong,et al.Utilization of modified BCR procedure for the chemical speciation of heavy metals in Chinese soil reference material[J].Ecology&Environmental Sciences,2012,21(11):1881-1884.
[22]刘铭,刘凤枝,刘保峰.土壤中有效态铅和镉的测定[J].农业环境科学学报,2007,26(S1):300-302.LIU Ming,LIU Feng-zhi,LIU Bao-feng.Determination of available lead and cadmium in soil[J].Journal of Agro-Environment Science,2007,26(S1):300-302.
[23]徐小逊,董袁媛,邓玉兰,等.镉胁迫对豨莶生长及光合作用相关参数的影响[J].农业环境科学学报,2016,35(9):1672-1679.XU Xiao-xun,DONG Yuan-yuan,DENG Yu-lan,et al.Effects of cadmium stress on growth and photosynthetic parameters of Sigesbeckia orientalis L.[J].Journal of Agro-Environment Science,2016,35(9):1672-1679.
[24]Cay S,Uyanik A,Engin M S.EDTA supported phytoextraction of Cd from contaminated soil by four different ornamental plant species[J].Journal of Soil Contamination,2016,25(3):346-355.
[25]张磊.螯合剂强化棉花对镉污染土壤修复的初步研究[J].水土保持学报,2015,29(4):321-326.ZHANG Lei.Preliminary study on chelate-enhanced phytoremediation of cadmium-contami nated soil by cotton plants[J].Journal of Soil and Water Conservation,2015,29(4):321-326.
[26]陈英旭,林琦,陆芳,等.有机酸对铅、镉植株危害的解毒作用研究[J].环境科学学报,2000,20(4):467-472.CHEN Ying-xu,LIN Qi,LU Fang,et al.Study on detoxication of organic acid to raddish under the stress of Pb and Cd[J].Acta Scientiae Circumstantiae,2000,20(4):467-472.
[27]夏小燕,杨丽琴,翟福勤,等.有机酸对小麦幼苗镉毒的缓解作用[J].农业环境科学学报,2007,26(3):990-995.XIA Xiao-yan,YANG Li-qin,ZHAI Fu-qin,et al.Organic acids alleviating the toxicity of Cd to young wheat plants[J].Journal of AgroEnvironment Science,2007,26(3):990-995.
[28]Zaheer I E,Ali S,Rizwan M,et al.Citric acid assisted phytoremediation of copper by Brassica napus L.[J].Ecotoxicology and Environmental Safety,2015,120:310-317.
[29]张玉芬,刘景辉,杨彦明,等.柠檬酸和EDTA对蓖麻生理特性和镉累积的影响[J].生态与农村环境学报,2015,31(5):760-766.ZHANG Yu-fen,LIU Jing-hui,YANG Yan-ming,et al.Effects of CA and EDTA on physiological characteristics and Cd accumulation of Ricinus communis[J].Journal of Ecology and Rural Environment,2015,31(5):760-766.
[30]胡国涛,杨兴,陈小米,等.速生树种竹柳对重金属胁迫的生理响应[J].环境科学学报,2016,36(10):3870-3875.HU Guo-tao,YANG Xing,CHEN Xiao-mi,et al.Physiological responses of bamboo-willow plants to heavy metal stress[J].Acta Scientiae Circumstantiae,2016,36(10):3870-3875.
[31]王思予,多立安,赵树兰.可降解螯合剂对草坪植物高羊茅发育及生理的影响[J].园艺学报,2017,44(11):2186-2194.WANG Si-yu,DUO Li-an,ZHAO Shu-lan.Effects of biodegradable chelator on growth and physiology of Festuca arundinacea seedlings[J].Acta Horticulturae Sinica,2017,44(11):2186-2194.
[32]Huang C,Lai C,Xu P,et al.Lead-induced oxidative stress and antioxidant response provide insight into the tolerance of Phanerochaete chrysosporium to lead exposure[J].Chemosphere,2017,187:70-77.
[33]Zhao Y,Mao G,Hu H,et al.Effects of EDTA and EDDS on heavy metal activation and accumulation of metals by soybean in alkaline soil[J].Journal of Soil Contamination,2015,24(4):353-367.
[34]Gao Y,Miao C,Xia J,et al.Effect of citric acid on phytoextraction and antioxidative defense in Solanum nigrum L.as a hyperaccumulator under Cd and Pb combined pollution[J].Environmental Earth Sciences,2012,65(7):1923-1932.
[35]Ehsan S,Ali S,Noureen S,et al.Citric acid assisted phytoremediation of cadmium by Brassica napus L.[J].Ecotoxicology&Environmental Safety,2014,106:164-172.
[36]熊国焕,潘义宏,何艳明,等.螯合剂对大叶井口边草Pb、Cd、As吸收性影响研究[J].土壤,2012,44(2):282-289.XIONG Guo-huan,PAN Yi-hong,HE Yan-ming,et al.Chelate assisted uptake of heavy metal of lead,cadmium and arsenic from soil with Pteris cretica var.nervosa[J].Soils,2012,44(2):282-289.
[37]张譞,李晔,胡进,等.三种螯合剂对土壤重金属Cd和Zn形态变化的研究[J].科学技术与工程,2013,13(21):6184-6188.ZHANG Xuan,LI Ye,HU Jin,et al.Effects of three chelate on the form distribution of Cd and Zn in soil[J].Science Technology and Engineering,2013,13(21):6184-6188.
[38]尹炳奎,黄满红,张大磊,等.菜籽饼施加对镉-铜污染土壤中重金属形态转化及其植物有效性的影响[J].环境工程学报,2017,11(6):3879-3883.YIN Bing-kui,HUANG Man-hong,ZHANG Da-lei,et al.Effects of rapeseed cake on cadmium and copper forms and its phytoavailability in heavy metals contaminated paddy soil[J].Chinese Journal of Environmental Engineering,2017,11(6):3879-3883.
[39]郑明霞,冯流,刘洁,等.螯合剂对土壤中镉赋存形态及其生物有效性的影响[J].环境化学,2007,26(5):606-609.ZHENG Ming-xia,FENG Liu,LIU Jie,et al.Effects of chelators on species and bioavailability of cadmium in soil[J].Environmental Chemistry,2007,26(5):606-609.
[40]李永涛,刘科学,张池,等.广东大宝山地区重金属污染水田土壤的Cu Pb Zn Cd全量与DTPA浸提态含量的相互关系研究[J].农业环境科学学报,2004,23(6):1110-1114.LI Yong-tao,LIU Ke-xue,ZHANG Chi,et al.Relationships between total and DTPA extractable contents of Cu,Pb,Zn,Cd in trace metalcontaminated paddy soils of Dabaoshan,Guangdong[J].Journal of Agro-environmental Science,2004,23(6):1110-1114.
[41]Luo J,Cai L,Qi S,et al.Improvement effects of cytokinin on EDTAassisted phytoremediation and the associated environmental risks[J].Chemosphere,2017,185:386-393.