蒙药圆柏的质量标准研究
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摘要
目的:建立蒙药圆柏的质量标准,为其质量控制提供参考。方法:收集内蒙古地区的圆柏药材,共9批。观察圆柏样品的性状并对其枝叶横切面和粉末进行显微鉴别;采用薄层色谱(TLC)法对圆柏样品进行定性分析;按2015年版《中国药典》(四部)通则方法测定圆柏样品中水分、总灰分、酸不溶性灰分含量和醇溶性浸出物含量;采用高效液相色谱法测定圆柏样品中槲皮苷和穗花杉双黄酮含量。结果:圆柏小枝呈圆柱形或四菱形,刺叶长短不等,呈深绿色或黄绿色;显微观察发现,鳞叶表皮细胞排列整齐、鳞叶中部有较大空腔、海绵组织细胞较大,表皮细胞类长方形,气孔较多,保卫细胞较大;TLC结果显示,在对照品(槲皮苷和芦丁)色谱相应位置上显相同颜色的荧光斑点;9批样品中水分含量为3.2%～5.6%,总灰分含量为3.4%～5.8%,酸不溶性灰分含量均不超过0.8%,醇溶性浸出物含量为28.0%～33.8%,槲皮苷含量为0.11%～0.28%,穗花杉双黄酮含量为0.15%～0.25%。结论:初步拟定圆柏中水分含量不得超过8.0%、总灰分含量不得超过7.0%、醇溶性浸出物含量不得低于24.0%、槲皮苷含量不得低于0.11%、穗花杉双黄酮含量不得低于0.15%,制订的标准可用于圆柏药材的质量控制。
OBJECTIVE:To establish the quality standard of Sabina vulgaris,and to provide reference for its quality control.METHODS:Totally 9 batches of S. vulgaris were collected from Inner Mongolia area. The characteristics of S. vulgaris samples were observed and microscopic identification was conducted for the transverse section and powder of its branches and leaves. TLC was used for qualitative analysis of S. vulgaris. The contents of water,total ash,acid-insoluble ash and alcohol-soluble extract in S.vulgaris were determined according to the method stated in 2015 edition of Chinese Pharmacopeia(part Ⅳ). The contents of quercetin and amentoflavone in S. vulgaris were determined by HPLC. RESULTS:The branchlets of S. vulgaris were cylindrical or rhombic,with varying length of thorns,dark green or yellowish green. The microscopic observation showed that the epidermal cells of scale leaves were arranged orderly;there were larger cavities in the middle of scale leaves,large cells in spongy tissue;epidermal cells were rectangular with more stomata and large guard cells. Results of TLC showed that the spots of the same color were found in the corresponding positions of chromatogram for substance control(dendrooside and rutin). In the 9 batch of samples,the contents of water were 3.2%-5.6%;the contents of total ash were 3.4%-5.8%;the contents of acid-insoluble ash were lower than 0.8%;the contents of alcohol-soluble extract were 28.0%-33.8%;the contents of quercetin were 0.11%-0.28%;the contents of amentoflavone were 0.15%-0.25%. CONCLUSIONS:It is preliminarily proposed that the content of water in S.vulgaris is not more than 8.0%;the content of total ash is not more than 7.0%;the content of alcohol-soluble extract is not less than 24.0%;the content of quercetin is not less than 0.11%;the content of amentoflavone is not less than 0.15%. Established standard can be used for the quality control of S. vulgaris.
OBJECTIVE:To establish the quality standard of Sabina vulgaris,and to provide reference for its quality control.METHODS:Totally 9 batches of S. vulgaris were collected from Inner Mongolia area. The characteristics of S. vulgaris samples were observed and microscopic identification was conducted for the transverse section and powder of its branches and leaves. TLC was used for qualitative analysis of S. vulgaris. The contents of water,total ash,acid-insoluble ash and alcohol-soluble extract in S.vulgaris were determined according to the method stated in 2015 edition of Chinese Pharmacopeia(part Ⅳ). The contents of quercetin and amentoflavone in S. vulgaris were determined by HPLC. RESULTS:The branchlets of S. vulgaris were cylindrical or rhombic,with varying length of thorns,dark green or yellowish green. The microscopic observation showed that the epidermal cells of scale leaves were arranged orderly;there were larger cavities in the middle of scale leaves,large cells in spongy tissue;epidermal cells were rectangular with more stomata and large guard cells. Results of TLC showed that the spots of the same color were found in the corresponding positions of chromatogram for substance control(dendrooside and rutin). In the 9 batch of samples,the contents of water were 3.2%-5.6%;the contents of total ash were 3.4%-5.8%;the contents of acid-insoluble ash were lower than 0.8%;the contents of alcohol-soluble extract were 28.0%-33.8%;the contents of quercetin were 0.11%-0.28%;the contents of amentoflavone were 0.15%-0.25%. CONCLUSIONS:It is preliminarily proposed that the content of water in S.vulgaris is not more than 8.0%;the content of total ash is not more than 7.0%;the content of alcohol-soluble extract is not less than 24.0%;the content of quercetin is not less than 0.11%;the content of amentoflavone is not less than 0.15%. Established standard can be used for the quality control of S. vulgaris.
引文
[1]中国科学院中国植物志编辑委员会.中国植物志:第七卷[M].北京:科学出版社,1978:359-360.
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[3]玛依拉·买买提依明,满尔哈巴·海如拉,于睿,等.新疆圆柏叶药材质量标准的探讨[J].时珍国医国药,2010,21(7):1686-1687.
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[7]李晨阳,高慧,陈燕,等. HPLC测定不同产地新疆圆柏中的槲皮苷[J].华西药学杂志,2015,30(6):720-721.
[8]李晨阳,高慧,陈燕,等.新疆圆柏叶中芦丁含量的测定[J].中国中医药信息杂志,2016,23(2):90-92.
[9]马志良,多杰.藏药悬钩木的质量标准研究[J].中国药房,2018,29(2):179-182.
[10]国家药典委员会.中华人民共和国药典:一部[S]. 2015年版.北京:中国医药科技出版社,2015:215-216.
[11]陈燕,李倩,李晨阳,等.不同产地、不同采收期新疆圆柏叶中穗花杉双黄酮的含量测定[J].新疆医科大学学报,2017,40(3):369-371.
[12]王彦志,张萌,刘阳,等.穗花杉双黄酮在大鼠体内的药动学研究[J].中成药,2015,11(37):2397-2401.
[13]陈燕,谭为,李晨阳,等.新疆圆柏叶的质量标准研究[J].中国民族民间医药,2016,25(16):14-16.
[2]内蒙古植物志编辑委员会.内蒙古植物志:第一卷[M].呼和浩特:内蒙古人民出版社,1985:146-147.
[3]玛依拉·买买提依明,满尔哈巴·海如拉,于睿,等.新疆圆柏叶药材质量标准的探讨[J].时珍国医国药,2010,21(7):1686-1687.
[4]国家药典委员会.中华人民共和国药典:四部[S]. 2015年版.北京:中国医药科技出版社,2015:103、202、204.
[5]赵军,闫明,黄毅,等.新疆圆柏黄酮类成分的研究[J].林产化学与工业,2008,43(19):33-37.
[6]武雪.藏药刺柏和圆柏药材质量标准的提高研究[D].兰州:兰州大学,2015:22-23.
[7]李晨阳,高慧,陈燕,等. HPLC测定不同产地新疆圆柏中的槲皮苷[J].华西药学杂志,2015,30(6):720-721.
[8]李晨阳,高慧,陈燕,等.新疆圆柏叶中芦丁含量的测定[J].中国中医药信息杂志,2016,23(2):90-92.
[9]马志良,多杰.藏药悬钩木的质量标准研究[J].中国药房,2018,29(2):179-182.
[10]国家药典委员会.中华人民共和国药典:一部[S]. 2015年版.北京:中国医药科技出版社,2015:215-216.
[11]陈燕,李倩,李晨阳,等.不同产地、不同采收期新疆圆柏叶中穗花杉双黄酮的含量测定[J].新疆医科大学学报,2017,40(3):369-371.
[12]王彦志,张萌,刘阳,等.穗花杉双黄酮在大鼠体内的药动学研究[J].中成药,2015,11(37):2397-2401.
[13]陈燕,谭为,李晨阳,等.新疆圆柏叶的质量标准研究[J].中国民族民间医药,2016,25(16):14-16.