蜂蜜中氨基脲本底来源研究
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摘要
目的建立液相色谱-串联质谱法测定蜂蜜中的氨基脲本底来源的分析方法。方法采用氨基脲非衍生检测方法和蜂蜜中呋喃类药物代谢物测定法,分别对包装垫圈及模拟性实验中蜂蜜的氨基脲含量进行测定。结果蜂蜜包装垫圈中检测出氨基脲,其含量范围25～3108.9μg/kg;进行模拟性实验的蜂蜜中氨基脲含量明显升高,最大升高含量为38.8μg/kg。结论蜂蜜中氨基脲本底来源与其商品包装材料中内溶物的扩散溶出有关。
Objective To establish a method for the determination of semicarbazide background sources in honey by liquid chromatography-tandem mass spectrometry(LC-MS/MS). Methods The semicarbazide non-derivatization test method and the furan drug metabolite assay in honey were used to determine the semicarbazide content of honey in packaging gaskets and simulated experiments. Results Semicarbazide was detected in honey packaging gaskets, and its content ranges from 25 to 3108.9 μg/kg. The content of semicarbazide in honey in the simulated experiment was significantly increased, and the maximum increase was 38.8 μg/kg.Conclusion The source of semicarbazide in honey is related to the diffusion and dissolution of internal dissolved matter in its packaging materials.
Objective To establish a method for the determination of semicarbazide background sources in honey by liquid chromatography-tandem mass spectrometry(LC-MS/MS). Methods The semicarbazide non-derivatization test method and the furan drug metabolite assay in honey were used to determine the semicarbazide content of honey in packaging gaskets and simulated experiments. Results Semicarbazide was detected in honey packaging gaskets, and its content ranges from 25 to 3108.9 μg/kg. The content of semicarbazide in honey in the simulated experiment was significantly increased, and the maximum increase was 38.8 μg/kg.Conclusion The source of semicarbazide in honey is related to the diffusion and dissolution of internal dissolved matter in its packaging materials.
引文
[1]祝伟霞,刘亚,梁炜,等动物性食品中硝基呋喃类药物残留检测研究进展[J].动物医学进展2010,31(2):99-102.Zhu WX,Liu Y,Liang W,et al.Progress on nitrofuran drug residues in animal-borne food[J].Prog Vet Med,2010,31(2):99-102.
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[3]Leitner A,Zolner P,Lindner W.Determination of themetabolites of nitrofuran antibiotics in animal tissue byhigh-performance liquid chromatography-tandem mass spectrometry[J].J Chromatogr A,2001,939(1):49-58.
[4]Fortieth report of the Joint FAO/WHO Expert Committee on Food Additives(JECFA).Evaluation of certain veterinary drug residues in food[R].WHO Technical Report Series:WHO,1993:32-40.
[5]中华人民共和国农业部.第193号公告.食品动物禁用的兽药及其它化合物清单[R].2011.Ministry of Agriculture of People's Republic of China.193th Announcement.List of veterinary drugs and other compounds prohibited by food animals[R].2011.
[6]Vass M,Hruska K,Franek M.Nitrofuran antibiotics:A review on the application,prohibition and residual analysis[J].Veter Med,2008,53(9):469-500.
[7]程波,舒秀君,宋蓓,等.甲壳类水产品氨基脲残留来源研究进展[J].广东海洋大学学报,2018,38(5):93-98.Cheng B,Shu XJ,Song P,et al.Advance in research on other sources that are linked neither to the illegal use of nitrofurazone in crustacean aquatic products[J].J Guangdong Ocean Univ,2018,38(5):93-98.
[8]周萍,胡福良,章征天,等.雄蜂蛹中硝基呋喃类代谢物含量的测定及超标原因分析[J].中国蜂业,2008,(7):5-6,9.Zhou P,Hu FL,Zhang ZT,et al.Determination of nitrofuran metabolites in drone pupae and reasons for their exceeding the standards[J].Apic China,2008,(7):5-6,9.
[9]Maria BDLC,Elke A.Semicarbazide:Occurrence in food products and state-of-the-art in analytical methods used for its determination[J].Anal Bioanal Chem,2005,382(4):968-977.
[10]Scheilla VC,de Souza RG,Junqueira,et al.Analysis of semicarbazide in baby food by liquid chromatography tandem mass spectrometry(LC-MS-MS)-In-house method validation and Richard Ginn[J].JChromatogr,2005,1077(2):151-158.
[11]Levai G,Nyitrai Z,Meszlenyi G.The kinetics and mechanism of thethermal decomposition of azodicarbonamide in polyethylene.II.The effect of zinc oxide[J].ACH-Models Chem,1999,(135):885-990.
[12]王文枝,陈志锋,孙利.包装食品中氨基脲风险评估[J].食品工业科技,2009,30(3):282-283,303.Wang WZ,Chen ZF,Sun L.Risk assessment of semicarbazide in packaged foods[J].Sci Technol Food Ind,2009,30(3):282-283,303
[13]Additional advice on semicarbazide,in particular related to baby food.EFSA-European Food Safety Authority,Panel on food additives,favourings,processing aids and materials in contact with food.http://www.efsa.eu.int/p foodadd en.html.2003.
[14]EFSA-European Food Safety Authority.http://www.efsa.eu.int/pdf/pressreal20031015en.pdf.2003.
[15]Stadler RH,Mottier P,Guy P,et al.Semicarbazide is a minor thermal decomposition product of azodicarbonamide used in the gaskets of certain food jars[J].Analyst,2004,(129):276-281.
[2]蒋原,丁涛,徐锦忠,等.硝基呋喃类药物在克氏螯虾组织中消除规律的研究畜牧与兽药[J].2008,40(2):34-37.Jiang Y,Ding T,Xu JZ,et al.Dynamical changes of Nitrfuran metabolites in crawfish[J].Anim Husb Vet Med,2008,40(2):34-37.
[3]Leitner A,Zolner P,Lindner W.Determination of themetabolites of nitrofuran antibiotics in animal tissue byhigh-performance liquid chromatography-tandem mass spectrometry[J].J Chromatogr A,2001,939(1):49-58.
[4]Fortieth report of the Joint FAO/WHO Expert Committee on Food Additives(JECFA).Evaluation of certain veterinary drug residues in food[R].WHO Technical Report Series:WHO,1993:32-40.
[5]中华人民共和国农业部.第193号公告.食品动物禁用的兽药及其它化合物清单[R].2011.Ministry of Agriculture of People's Republic of China.193th Announcement.List of veterinary drugs and other compounds prohibited by food animals[R].2011.
[6]Vass M,Hruska K,Franek M.Nitrofuran antibiotics:A review on the application,prohibition and residual analysis[J].Veter Med,2008,53(9):469-500.
[7]程波,舒秀君,宋蓓,等.甲壳类水产品氨基脲残留来源研究进展[J].广东海洋大学学报,2018,38(5):93-98.Cheng B,Shu XJ,Song P,et al.Advance in research on other sources that are linked neither to the illegal use of nitrofurazone in crustacean aquatic products[J].J Guangdong Ocean Univ,2018,38(5):93-98.
[8]周萍,胡福良,章征天,等.雄蜂蛹中硝基呋喃类代谢物含量的测定及超标原因分析[J].中国蜂业,2008,(7):5-6,9.Zhou P,Hu FL,Zhang ZT,et al.Determination of nitrofuran metabolites in drone pupae and reasons for their exceeding the standards[J].Apic China,2008,(7):5-6,9.
[9]Maria BDLC,Elke A.Semicarbazide:Occurrence in food products and state-of-the-art in analytical methods used for its determination[J].Anal Bioanal Chem,2005,382(4):968-977.
[10]Scheilla VC,de Souza RG,Junqueira,et al.Analysis of semicarbazide in baby food by liquid chromatography tandem mass spectrometry(LC-MS-MS)-In-house method validation and Richard Ginn[J].JChromatogr,2005,1077(2):151-158.
[11]Levai G,Nyitrai Z,Meszlenyi G.The kinetics and mechanism of thethermal decomposition of azodicarbonamide in polyethylene.II.The effect of zinc oxide[J].ACH-Models Chem,1999,(135):885-990.
[12]王文枝,陈志锋,孙利.包装食品中氨基脲风险评估[J].食品工业科技,2009,30(3):282-283,303.Wang WZ,Chen ZF,Sun L.Risk assessment of semicarbazide in packaged foods[J].Sci Technol Food Ind,2009,30(3):282-283,303
[13]Additional advice on semicarbazide,in particular related to baby food.EFSA-European Food Safety Authority,Panel on food additives,favourings,processing aids and materials in contact with food.http://www.efsa.eu.int/p foodadd en.html.2003.
[14]EFSA-European Food Safety Authority.http://www.efsa.eu.int/pdf/pressreal20031015en.pdf.2003.
[15]Stadler RH,Mottier P,Guy P,et al.Semicarbazide is a minor thermal decomposition product of azodicarbonamide used in the gaskets of certain food jars[J].Analyst,2004,(129):276-281.